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Your search keyword '"Wei, Jin"' showing total 4 results
4 results on '"Wei, Jin"'

1. Inhibition of Snail Family Transcriptional Repressor 2 (SNAI2) Enhances Multidrug Resistance of Hepatocellular Carcinoma Cells

Author

Lei Huang, Lei Li, Ke-Wen Zhao, Yaping Lv, Xiao-Bo Wang, Guo-Qiang Chen, Wei Jin, Rong-Jie Fu, Xin-Yu Zhao, and Chao Meng

Subjects
0301 basic medicine, Transcription, Genetic, Carcinogenesis, Molecular biology, Cancer Treatment, Gene Expression, lcsh:Medicine, ATP-binding cassette transporter, Mice, Animal Cells, Cancer Stem Cells, Medicine and Health Sciences, Neoplasm Metastasis, RNA, Small Interfering, lcsh:Science, Multidisciplinary, Pharmaceutics, Liver Diseases, Stem Cells, Liver Neoplasms, Drug Resistance, Multiple, Up-Regulation, Oncology, Optical Equipment, Neoplastic Stem Cells, Engineering and Technology, Female, Efflux, Cellular Types, Liver cancer, Research Article, Clinical Oncology, Carcinoma, Hepatocellular, Epithelial-Mesenchymal Transition, DNA transcription, Equipment, Gastroenterology and Hepatology, Biology, DNA construction, Carcinomas, 03 medical and health sciences, Drug Therapy, Cell Line, Tumor, Gastrointestinal Tumors, medicine, Genetics, Gene silencing, Chemotherapy, Animals, Humans, Epithelial–mesenchymal transition, Gene Silencing, Transcription factor, Cell Proliferation, lcsh:R, Cancer, Cancers and Neoplasms, Biology and Life Sciences, Prisms, Hepatocellular Carcinoma, Cell Biology, medicine.disease, Research and analysis methods, SNAI2, 030104 developmental biology, Molecular biology techniques, Verapamil, Plasmid Construction, Cancer research, ATP-Binding Cassette Transporters, lcsh:Q, Snail Family Transcription Factors, Clinical Medicine
Abstract

China accounts for almost half of the total number of liver cancer cases and deaths worldwide, and hepatocellular carcinoma (HCC) is the most primary liver cancer. Snail family transcriptional repressor 2 (SNAI2) is known as an epithelial to mesenchymal transition-inducing transcription factor that drives neoplastic epithelial cells into mesenchymal phenotype. However, the roles of endogenous SNAI2 remain controversial in different types of malignant tumors. Herein, we surprisingly identify that anchorage-independent growth, including the formation of tumor sphere and soft agar colony, is significantly increased when SNAI2 expression is inhibited by shRNAs in HCC cells. Suppression of SNAI2 suffices to up-regulate several cancer stem genes. Although unrelated to the metastatic ability, SNAI2 inhibition does increase the efflux of Hoechst 33342 and enhance multidrug resistance in vitro and in vivo. In agreement with this data, we demonstrate for the first time that decreasing SNAI2 level can transcriptionally upregulate several ATP binding cassette (ABC) transporter genes such as ABCB1. Moreover, ABC transporters' inhibitor verapamil can rescue the multidrug resistance induced by SNAI2 inhibition. Our results implicate that SNAI2 behaves as a tumor suppressor by inhibiting multidrug resistance via suppressing ABC transporter genes in HCC cells.

Published
2016

2. Application of carbon nanoparticles in laparoscopic sentinel lymph node detection in patients with early-stage cervical cancer.

Author

Lu, Yan, Wei, Jin-Ying, Yao, De-Sheng, Pan, Zhong-Mian, and Yao, Yao

Subjects
*SENTINEL lymph nodes, *NANOPARTICLES, *CERVICAL cancer patients, *IMMUNOSTAINING, *ARTIFICIAL palates
Abstract

Objective: To investigate the value of carbon nanoparticles in identifying sentinel lymph nodes in early-stage cervical cancer. Methods: From January 2014 to January 2016, 40 patients with cervical cancer stage IA2–IIA, based on the International Federation of Gynecology and Obstetrics (FIGO) 2009 criteria, were included in this study. The normal cervix around the tumor was injected with a total of 1 mL of carbon nanoparticles (CNP)at 3 and 9 o'clock. All patients then underwent laparoscopic pelvic lymph node dissection and radical hysterectomy. The black-dyed sentinel lymph nodes were removed for routine pathological examination and immunohistochemical staining. Results: Among the 40 patients, 38 patients had at least one sentinel lymph node (SLN). The detection rate was 95% (38/40). One hundred seventy-three SLNs were detected with an average of 3.9 SLNs per side. 25 positive lymph nodes, which included 21 positive SLNs, were detected in 8 (20%) patients. Sentinel lymph nodes were localized in the obturator (47.97%), internal lilac (13.87%), external lilac (26.59%), parametrial (1.16%), and common iliac (8.67%) regions. The sensitivity of the SLN detection was 100% (5/5), the accuracy was 97.37% (37/38), and the negative predictive value was 100. 0% and the false negative rate was 0%. Conclusions: Sentinel lymph nodes can be used to accurately predict the pathological state of pelvic lymph nodes in early cervical cancer. The detection rates and accuracy of sentinel lymph node were high. Carbon nanoparticles can be used to trace the sentinel lymph node in early cervical cancer. [ABSTRACT FROM AUTHOR]

Published
2017
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3. Autophagy inhibition enhances daunorubicin-induced apoptosis in K562 cells

Author

Kaifeng Wang, Da Li, Qin Pan, Hongming Pan, Yan Chen, Lifeng Feng, Wei Jin, Weidong Han, Jie Sun, Xian Wang, and Hongchuan Jin

Subjects
Cancer Treatment, lcsh:Medicine, Apoptosis, Signal transduction, ERK signaling cascade, Hematologic Cancers and Related Disorders, Molecular cell biology, Neoplasms, Basic Cancer Research, RNA, Small Interfering, Extracellular Signal-Regulated MAP Kinases, lcsh:Science, health care economics and organizations, Antibiotics, Antineoplastic, Microscopy, Confocal, Multidisciplinary, Signaling cascades, Myeloid leukemia, Chloroquine, Drug Synergism, Hematology, humanities, Leukemia, Oncology, Leukemia, Myeloid, Antirheumatic Agents, Medicine, Research Article, medicine.drug, Acute Myeloid Leukemia, Programmed cell death, Daunorubicin, ATG5, Chronic Myeloid Leukemia, Biology, Microscopy, Electron, Transmission, Leukemias, Autophagy, medicine, Humans, Myeloproliferative Disorders, lcsh:R, Cancers and Neoplasms, Chemotherapy and Drug Treatment, medicine.disease, Cancer cell, Cancer research, lcsh:Q, K562 Cells, K562 cells
Abstract

Anthracycline daunorubicin (DNR) is one of the major antitumor agents widely used in the treatment of myeloid leukemia. Unfortunately, the clinical efficacy of DNR was limited because of its cytotoxity at high dosage. As a novel cytoprotective mechanism for tumor cell to survive under unfavorable conditions, autophagy has been proposed to play a role in drug resistance of tumor cells. Whether DNR can activate to impair the sensitivity of cancer cells remains unknown. Here, we first report that DNR can induce a high level of autophagy, which was associated with the activation of extracellular signal-regulated kinase 1/2 (ERK1/2). Moreover, cell death induced by DNR was greatly enhanced after autophagy inhibition by the pharmacological inhibitor chloroquine (CQ) and siRNAs targeting Atg5 and Atg7, the most important components for the formation of autophagosome. In conclusion, we found that DNR can induce cytoprotective autophagy by activation of ERK in myeloid leukemia cells. Autophagy inhibition thus represents a promising approach to improve the efficacy of DNR in the treatment of patients with myeloid leukemia.

Published
2011

4. Multidisciplinary Modalities Achieve Encouraging Long-Term Survival in Resectable Limited-Disease Esophageal Small Cell Carcinoma.

Author

Hou, Xue, Wei, Jin-Chang, Wu, Jing-Xun, Wang, Xin, Fu, Jian-Hua, Lin, Peng, and Yang, Hao-Xian

Subjects
*LONG-term care facilities, *SMALL cell carcinoma, *SURGICAL excision, *DISEASE management, *TREATMENT of esophageal cancer, *ESOPHAGEAL cancer patients
Abstract

Background: The management of limited-disease esophageal small cell carcinoma is not well defined, and the role of surgery is still controversial. We aim to determine the optimal treatment strategy in limited-disease of esophageal small cell carcinoma. Methods and Findings: We conducted a retrospective review of 141 patients with limited-disease esophageal small cell carcinoma from 3 institutions in China who underwent treatment between July 1994 and September 2008, July 1994 and July 2011, and June 2004 and December 2010, respectively. The survival rate was calculated by the Kaplan-Meier method, and the log-rank test was used to assess the survival differences between the groups. Cox proportional hazards model were used to further determine the independent factors impacting overall survival. The median survival time was 16.1 months for the entire cohort of patients, with a 5-year survival rate of 6.7%. The median survival times for surgery alone, surgery combined with chemotherapy, surgery combined with radiotherapy, surgery combined with chemotherapy and radiotherapy, chemotherapy plus radiotherapy, and chemotherapy alone were 18.0 months, 15.0 months, 23.0 months, 25.0 months, 17.1 months, and 6.1 months, respectively; the corresponding 5-year survival rates were 0%, 15.4%, 0%, 38.9%, 0%, and 0%, respectively. For the 105 patients who underwent R0 resection, the median disease-free survival time was 12.0 months, with a 95% confidence interval of 9.5 months to 14.5 months. The multivariate Cox regression analysis demonstrated that advanced pathological staging (p = 0.003), and pure esophageal small cell carcinoma (p = 0.035) were independent factors decreasing overall survival. Conclusions: Our data suggested that multidisciplinary modalities achieved encouraging long-term survival in patients with resectable limited-disease of esophageal small cell carcinoma. [ABSTRACT FROM AUTHOR]

Published
2013
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