1. Enhanced cell proliferation and osteogenic differentiation in electrospun PLGA/hydroxyapatite nanofibre scaffolds incorporated with graphene oxide
- Author
-
Jianan Li, Yunshen Bai, Jiaqi Zhu, Zhihao Niu, Chuan Fu, Xiao-Yu Yang, Haotian Bai, and Yu Wang
- Subjects
Polymers ,Nanofibers ,Gene Expression ,lcsh:Medicine ,02 engineering and technology ,Bone tissue ,Physical Chemistry ,01 natural sciences ,Mice ,chemistry.chemical_compound ,Polylactic Acid-Polyglycolic Acid Copolymer ,Tissue engineering ,Osteogenesis ,Nanotechnology ,lcsh:Science ,Multidisciplinary ,Tissue Scaffolds ,Cell Differentiation ,3T3 Cells ,Adhesion ,021001 nanoscience & nanotechnology ,Electrospinning ,Extracellular Matrix ,Chemistry ,PLGA ,medicine.anatomical_structure ,Macromolecules ,Physical Sciences ,Sorption ,Engineering and Technology ,Graphite ,Cellular Structures and Organelles ,0210 nano-technology ,Research Article ,Biotechnology ,Biocompatibility ,Materials by Structure ,Materials Science ,Material Properties ,Bioengineering ,010402 general chemistry ,Tensile Strength ,Genetics ,medicine ,Mechanical Properties ,Animals ,Lactic Acid ,Materials by Attribute ,Nanomaterials ,Cell Proliferation ,Tissue Engineering ,lcsh:R ,technology, industry, and agriculture ,Biology and Life Sciences ,Cell Biology ,Polymer Chemistry ,0104 chemical sciences ,Durapatite ,chemistry ,Chemical engineering ,Nanofiber ,lcsh:Q ,Adsorption ,Polyglycolic Acid ,Developmental Biology ,Protein adsorption - Abstract
One of the goals of bone tissue engineering is to mimic native ECM in architecture and function, creating scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties. The aim of this study was to fabricate nanofibrous matrices by electrospinning a blend of poly (L-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and grapheme oxide (GO) as a favourable platform for bone tissue engineering. The morphology, biocompatibility, mechanical properties, and biological activity of all nanofibrous matrices were compared. The data indicate that the hydrophilicity and protein adsorption rate of the fabricated matrices were significantly increased by blending with a small amount of HA and GO. Furthermore, GO significantly boosted the tensile strength of the nanofibrous matrices, and the PLGA/GO/HA nanofibrous matrices can serve as mechanically stable scaffolds for cell growth. For further test in vitro, MC3T3-E1 cells were cultured on the PLGA/HA/GO nanofbrous matrices to observe various cellular activities and cell mineralization. The results indicated that the PLGA/GO/HA nanofibrous matrices significantly enhanced adhesion, and proliferation in MCET3-E1 cells and functionally promoted alkaline phosphatase (ALP) activity, the osteogenesis-related gene expression and mineral deposition. Therefore, the PLGA/HA/GO composite nanofibres are excellent and versatile scaffolds for applications in bone tissue regeneration.
- Published
- 2017