1. Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells
- Author
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Wei Gao, Jin-Yu Zhang, Xinyao Feng, Chang-Jiang Wang, Ze-Wen Yu, Jian Chen, Jin-Ping Hu, Hai-Xiang Guo, Bao Yuan, and Wen-Zhi Ren
- Subjects
Male ,0301 basic medicine ,Pituitary gland ,Physiology ,Peptide Hormones ,Gene Expression ,Apoptosis ,Nervous System ,Biochemistry ,Rats, Sprague-Dawley ,0302 clinical medicine ,Gene expression ,Medicine and Health Sciences ,3' Untranslated Regions ,Multidisciplinary ,Cell Death ,Transfection ,Growth hormone secretion ,Cell biology ,Nucleic acids ,medicine.anatomical_structure ,Cell Processes ,Pituitary Gland ,030220 oncology & carcinogenesis ,Medicine ,Anatomy ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,endocrine system ,Science ,Primary Cell Culture ,Endocrine System ,DNA construction ,Biology ,Research and Analysis Methods ,Cell Line ,03 medical and health sciences ,Anterior pituitary ,Pituitary Gland, Anterior ,Pituitary Hormones, Anterior ,microRNA ,Genetics ,medicine ,Animals ,Secretion ,RNA, Messenger ,Non-coding RNA ,Molecular Biology Techniques ,Molecular Biology ,Natural antisense transcripts ,Biology and Life Sciences ,Cell Biology ,Hormones ,Gene regulation ,Rats ,Neuroanatomy ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,Cell culture ,Growth Hormone ,Plasmid Construction ,RNA ,Physiological Processes ,Neuroscience - Abstract
Growth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.
- Published
- 2019