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5. Spontaneous production of immunoglobulin M in human epithelial cancer cells

Author

Li Geng, Wenwei Shao, Qinyuan Liao, Jie Zheng, Fanlei Hu, Teng Ma, Jing Huang, Ling Zhao, Xiaoyan Qiu, Li Zhang, and Cheng Cameron Yin

Subjects
Antibodies, Neoplasm, Immune Cells, Immunology, Immunoglobulins, Receptors, Antigen, B-Cell, lcsh:Medicine, Autoantigens, HT29 Cells, Antigen, Cell Line, Tumor, Neoplasms, Molecular Cell Biology, Basic Cancer Research, Humans, Antibody-Producing Cells, lcsh:Science, Biology, B-Lymphocytes, Multidisciplinary, biology, HEK 293 cells, lcsh:R, Epithelial Cells, Chloroquine, Immunohistochemistry, Molecular biology, Raji cell, B-1 cell, Oncology, Immunoglobulin M, Oligodeoxyribonucleotides, Tissue Array Analysis, Cell culture, Gene Knockdown Techniques, Toll-Like Receptor 9, Myeloid Differentiation Factor 88, biology.protein, Medicine, lcsh:Q, Cellular Types, Antibody, Research Article
Abstract

It is well known that B-1 B cells are the main cell type that is responsible for the production of natural immunoglobulin M (IgM) and can respond to infection by increasing IgM secretion. However, we unexpectedly found that some epithelial cells also can express rearranged IgM transcript that has natural IgM characteristics, such as germline-encoded and restricted rearrangement patterns. Here we studied IgM expression in human non-B cells and found that IgM was frequently expressed by many human epithelial cancer cells as well as non-cancer epithelial cells. Moreover, CD79A and CD79B, two molecules that are physically linked to membranous IgM on the surface of B cells to form the B cell antigen receptor complex, were also expressed on the cell surface of epithelial cancer cells and co-located with IgM. Like the natural IgM, the epithelial cancer cell-derived IgM recognized a series of microbial antigens, such as single-stranded DNA, double-stranded DNA, lipopolysaccharide, and the HEp-2 cell antigen. More important, stimulation of the toll-like receptor 9 (TLR9), which mimics bacterial infection, substantially increased the secretion of IgM in human epithelial cancer cells. These findings indicate that human epithelial cancer cells as well as non-cancer epithelial cells can spontaneously produce IgM with natural antibody activity.

Published
2012

10. Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells.

Author

Fanlei Hu, Li Zhang, Jie Zheng, Ling Zhao, Jing Huang, Wenwei Shao, Qinyuan Liao, Teng Ma, Li Geng, C. Cameron Yin, and Xiaoyan Qiu

Subjects
CARCINOMA, IMMUNOGLOBULIN M, GERM cells, DNA, ANTIGEN receptors, B cells
Abstract

It is well known that B-1 B cells are the main cell type that is responsible for the production of natural immunoglobulin M (IgM) and can respond to infection by increasing IgM secretion. However, we unexpectedly found that some epithelial cells also can express rearranged IgM transcript that has natural IgM characteristics, such as germline-encoded and restricted rearrangement patterns. Here we studied IgM expression in human non-B cells and found that IgM was frequently expressed by many human epithelial cancer cells as well as non-cancer epithelial cells. Moreover, CD79A and CD79B, two molecules that are physically linked to membranous IgM on the surface of B cells to form the B cell antigen receptor complex, were also expressed on the cell surface of epithelial cancer cells and co-located with IgM. Like the natural IgM, the epithelial cancer cell-derived IgM recognized a series of microbial antigens, such as single-stranded DNA, double-stranded DNA, lipopolysaccharide, and the HEp-2 cell antigen. More important, stimulation of the toll-like receptor 9 (TLR9), which mimics bacterial infection, substantially increased the secretion of IgM in human epithelial cancer cells. These findings indicate that human epithelial cancer cells as well as non-cancer epithelial cells can spontaneously produce IgM with natural antibody activity. [ABSTRACT FROM AUTHOR]

Published
2012
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