1. Alcohol Dehydrogenase Protects against Endoplasmic Reticulum Stress-Induced Myocardial Contractile Dysfunction via Attenuation of Oxidative Stress and Autophagy: Role of PTEN-Akt-mTOR Signaling
- Author
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Rui Guo, Nathan D. Fuller, Nan Hu, Jeremy M. Henion, Jiaojiao Pang, Linzi A. Barton, Jun Ren, and Yuguo Chen
- Subjects
0301 basic medicine ,lcsh:Medicine ,Cardiovascular Medicine ,medicine.disease_cause ,Endoplasmic Reticulum ,Biochemistry ,Mice ,Ventricular Dysfunction, Left ,Cell Signaling ,Medicine and Health Sciences ,Transgenes ,Phosphorylation ,Post-Translational Modification ,lcsh:Science ,Ultrasonography ,Multidisciplinary ,Secretory Pathway ,Cell Death ,TOR Serine-Threonine Kinases ,Tunicamycin ,Physics ,Classical Mechanics ,Animal Models ,Endoplasmic Reticulum Stress ,Signaling Cascades ,Cell biology ,Cell Processes ,Cardiovascular Diseases ,Physical Sciences ,Mechanical Stress ,Signal transduction ,Cellular Structures and Organelles ,Signal Transduction ,Research Article ,MAP Kinase Signaling System ,Recombinant Fusion Proteins ,Autophagic Cell Death ,ATG5 ,Mice, Inbred Strains ,Mice, Transgenic ,Mouse Models ,Biology ,Research and Analysis Methods ,Stress Signaling Cascade ,03 medical and health sciences ,Model Organisms ,medicine ,Autophagy ,Animals ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Sirolimus ,Endoplasmic reticulum ,Adenine ,Myocardium ,lcsh:R ,Alcohol Dehydrogenase ,PTEN Phosphohydrolase ,Biology and Life Sciences ,Proteins ,Cell Biology ,Myocardial Contraction ,Oxidative Stress ,030104 developmental biology ,Unfolded protein response ,lcsh:Q ,Calcium ,Protein Processing, Post-Translational ,Proto-Oncogene Proteins c-akt ,Oxidative stress - Abstract
Background The endoplasmic reticulum (ER) plays an essential role in ensuring proper folding of the newly synthesized proteins. Aberrant ER homeostasis triggers ER stress and development of cardiovascular diseases. ADH is involved in catalyzing ethanol to acetaldehyde although its role in cardiovascular diseases other than ethanol metabolism still remains elusive. This study was designed to examine the impact of ADH on ER stress-induced cardiac anomalies and underlying mechanisms involved using cardiac-specific overexpression of alcohol dehydrogenase (ADH). Methods ADH and wild-type FVB mice were subjected to the ER stress inducer tunicamycin (1 mg/kg, i.p., for 48 hrs). Myocardial mechanical and intracellular Ca(2+) properties, ER stress, autophagy and associated cell signaling molecules were evaluated. Results ER stress compromised cardiac contractile function (evidenced as reduced fractional shortening, peak shortening, maximal velocity of shortening/relengthening, prolonged relengthening duration and impaired intracellular Ca(2+) homeostasis), oxidative stress and upregulated autophagy (increased LC3B, Atg5, Atg7 and p62), along with dephosphorylation of PTEN, Akt and mTOR, all of which were attenuated by ADH. In vitro study revealed that ER stress-induced cardiomyocyte anomaly was abrogated by ADH overexpression or autophagy inhibition using 3-MA. Interestingly, the beneficial effect of ADH was obliterated by autophagy induction, inhibition of Akt and mTOR. ER stress also promoted phosphorylation of the stress signaling ERK and JNK, the effect of which was unaffected by ADH transgene. Conclusions Taken together, these findings suggested that ADH protects against ER stress-induced cardiac anomalies possibly via attenuation of oxidative stress and PTEN/Akt/mTOR pathway-regulated autophagy.
- Published
- 2015