1. Cardiosphere-Derived Cells Facilitate Heart Repair by Modulating M1/M2 Macrophage Polarization and Neutrophil Recruitment
- Author
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Al Shaimaa Hasan, Shinji Goto, Tao-Sheng Li, Shouhua Zhang, Yoshishige Urata, Safwat A. Mangoura, Chen Yan, Mahmoud H. Abdel-Raheem, Lan Luo, and Tian-Xia Zhang
- Subjects
0301 basic medicine ,Male ,Neutrophils ,Myocardial Infarction ,lcsh:Medicine ,Apoptosis ,030204 cardiovascular system & hematology ,Pathology and Laboratory Medicine ,White Blood Cells ,Mice ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Myocardial infarction ,lcsh:Science ,Immune Response ,Cells, Cultured ,Staining ,Multidisciplinary ,Cell Death ,Cell Differentiation ,Heart ,M2 Macrophage ,Specimen preparation and treatment ,Neutrophil Infiltration ,Infarction ,Cell Processes ,Animal studies ,Cellular Types ,Anatomy ,Stem cell ,medicine.symptom ,Mannose Receptor ,Research Article ,Immune Cells ,Immunology ,Cardiology ,Inflammation ,Receptors, Cell Surface ,Biology ,Mesenchymal Stem Cell Transplantation ,Andrology ,03 medical and health sciences ,Signs and Symptoms ,Diagnostic Medicine ,medicine ,Animals ,Regeneration ,Lectins, C-Type ,CD86 ,Blood Cells ,Macrophages ,Myocardium ,lcsh:R ,DAPI staining ,Biology and Life Sciences ,Correction ,Mesenchymal Stem Cells ,Cell Biology ,Fibroblasts ,medicine.disease ,Research and analysis methods ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,Mannose-Binding Lectins ,Culture Media, Conditioned ,Nuclear staining ,Cardiovascular Anatomy ,lcsh:Q ,B7-2 Antigen ,Immunostaining - Abstract
Cardiosphere-derived cells (CDCs), one of the promising stem cell sources for myocardial repair, have been tested in clinical trials and resulted in beneficial effects; however, the relevant mechanisms are not fully understood. In this study, we examined the hypothesis that CDCs favor heart repair by switching the macrophages from a pro-inflammatory phenotype (M1) into a regulatory anti-inflammatory phenotype (M2). Macrophages from mice were cultured with CDCs-conditioned medium or with fibroblasts-conditioned medium as a control. Immunostaining showed that CDCs-conditioned medium significantly enhanced the expression of CD206 (a marker for M2 macrophages), but decreased the expression of CD86 (a marker for M1 macrophages) 3 days after culture. For animal studies, we used an acute myocardial infarction model of mice. We injected CDCs, fibroblasts, or saline only into the border zone of infarction. Then we collected the heart tissues for histological analysis 5 and 14 days after treatment. Compared with control animals, CDCs treatment significantly decreased M1 macrophages and neutrophils but increased M2 macrophages in the infarcted heart. Furthermore, CDCs-treated mice had reduced infarct size and fewer apoptotic cells compared to the controls. Our data suggest that CDCs facilitate heart repair by modulating M1/M2 macrophage polarization and neutrophil recruitment, which may provide a new insight into the mechanisms of stem cell-based myocardial repair., PLOS ONE, 11(10), e0165255; 2016
- Published
- 2016