1. Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum
- Author
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Xiao-xue Lu, Jianping Xie, Yu-yang Feng, Xin-li Ye, Zhu-jun Zhang, Qian Li, Xuhu Mao, Guo-dong Dun, Ya-nan Tong, Bin Tang, and Dong-zhu Zeng
- Subjects
0301 basic medicine ,Male ,Microarrays ,Cell Lines ,Artificial Gene Amplification and Extension ,Biochemistry ,Polymerase Chain Reaction ,Transcriptome ,0302 clinical medicine ,Medicine and Health Sciences ,Regulation of gene expression ,Multidisciplinary ,biology ,Gene Ontologies ,Genomics ,Middle Aged ,CREB-Binding Protein ,Enzymes ,Gene Expression Regulation, Neoplastic ,Nucleic acids ,Bioassays and Physiological Analysis ,Oncology ,030220 oncology & carcinogenesis ,Medicine ,Female ,Biological Cultures ,Colorectal Neoplasms ,Oxidoreductases ,Luciferase ,Signal Transduction ,Research Article ,Adult ,Science ,Research and Analysis Methods ,03 medical and health sciences ,Young Adult ,stomatognathic system ,microRNA ,medicine ,Genetics ,Humans ,CREB-binding protein ,Non-coding RNA ,Molecular Biology Techniques ,Molecular Biology ,Colorectal Cancer ,Natural antisense transcripts ,Fusobacterium nucleatum ,Biology and life sciences ,Microarray analysis techniques ,Cancer ,Cancers and Neoplasms ,Proteins ,Computational Biology ,Reverse Transcriptase-Polymerase Chain Reaction ,biology.organism_classification ,medicine.disease ,Genome Analysis ,Gene regulation ,MicroRNAs ,stomatognathic diseases ,030104 developmental biology ,Case-Control Studies ,biology.protein ,Gene chip analysis ,Cancer research ,Fusobacterium Infections ,Enzymology ,RNA ,Gene expression ,Caco-2 Cells - Abstract
Colorectal cancer (CRC) is a common and highly lethal form of cancer. Although the etiologic role of Fusobacterium nucleatum (F. nucleatum) in the development of CRC has been elucidated, the specific tumor molecules involved in the progression of CRC induced by F. nucleatum have not been identified. This study investigated several miRNAs and genes involved in the progression of F. nucleatum-induced CRC by Affymetrix miRNA microarray technology and GeneChip Human Transcriptome Array 2.0. The results suggest that miR-4474 and miR-4717 are up-regulated in CRC tissues in response to F. nucleatum infection, compared with the control group (paracancerous tissues), while other genes associated with signaling pathways in cancer, including CREB-binding protein (CREBBP), STAT1, PRKACB, CAMK2B, JUN, TP53 and EWSR1, were dysregulated. Bioinformatic analysis identified CREBBP as the primary aberrantly expressed gene in F. nucleatum-induced CRC. Consistent with the microarray analysis results, real-time RT-PCR analysis demonstrated that the expression of miR-4474/4717 was upregulated while that of CREBBP mRNA was downregulated in CRC patients infected with F. nucleatum. Additionally, CREBBP was identified as a novel target of miR-4474/4717. The results of this study suggest that miR-4474 and miR-4717 are involved in the progression of F. nucleatum-induced CRC by posttranscriptionally regulating the target gene CREBBP.
- Published
- 2019