1. Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy
- Author
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Qing Huang, Qianqian Zhang, Da Wei, Ma Yuhan, Zhu Chen, He Huaqi, Mingguang Kong, Zhao Yan, and Qifu Zhang
- Subjects
0106 biological sciences ,0301 basic medicine ,Fungal Structure ,Plasma Gases ,Spectrophotometry, Infrared ,Ganoderma ,Molecular biology ,Glycobiology ,Mutagenesis (molecular biology technique) ,lcsh:Medicine ,Infrared Spectroscopy ,Mycology ,Polysaccharide ,Research and Analysis Methods ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,Spectrum Analysis Techniques ,Polysaccharides ,010608 biotechnology ,Genetics ,Food science ,Biomass ,lcsh:Science ,Mycelium ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Strain (chemistry) ,lcsh:R ,Biology and Life Sciences ,near-Infrared Spectroscopy ,Absorption Spectroscopy ,Protoplast ,biology.organism_classification ,RAPD ,Random Amplified Polymorphic DNA Technique ,Mutant Strains ,Artificial gene amplification and extension ,030104 developmental biology ,Molecular biology techniques ,chemistry ,Mutagenesis ,Mutation ,Fermentation ,lcsh:Q ,Research Article - Abstract
As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.
- Published
- 2018