Search

Your search keyword '"Adam Nopora"' showing total 2 results
Start Over Author "Adam Nopora" Journal plos one
2 results on '"Adam Nopora"'

1. Targeting Tumor Cells with Anti-CD44 Antibody Triggers Macrophage-Mediated Immune Modulatory Effects in a Cancer Xenograft Model.

Author

Daniela Maisel, Fabian Birzele, Edgar Voss, Adam Nopora, Sabine Bader, Thomas Friess, Bernhard Goller, Daphna Laifenfeld, Stefan Weigand, and Valeria Runza

Subjects
Medicine, Science
Abstract

CD44, a transmembrane receptor reported to be involved in various cellular functions, is overexpressed in several cancer types and supposed to be involved in the initiation, progression and prognosis of these cancers. Since the sequence of events following the blockage of the CD44-HA interaction has not yet been studied in detail, we profiled xenograft tumors by RNA Sequencing to elucidate the mode of action of the anti-CD44 antibody RG7356. Analysis of tumor and host gene-expression profiles led us to the hypothesis that treatment with RG7356 antibody leads to an activation of the immune system. Using cytokine measurements we further show that this activation involves the secretion of chemo-attractants necessary for the recruitment of immune cells (i.e. macrophages) to the tumor site. We finally provide evidence for antibody-dependent cellular phagocytosis (ADCP) of the malignant cells by macrophages.

Published
2016
Full Text
View/download PDF

2. Targeting Tumor Cells with Anti-CD44 Antibody Triggers Macrophage-Mediated Immune Modulatory Effects in a Cancer Xenograft Model

Author

Edgar Voss, Adam Nopora, Bernhard Goller, Daphna Laifenfeld, Thomas Friess, Sabine Bader, Valeria Runza, Daniela Maisel, Stefan Weigand, and Fabian Birzele

Subjects
0301 basic medicine, Physiology, Molecular biology, medicine.medical_treatment, Cancer Treatment, lcsh:Medicine, Mice, SCID, Mice, White Blood Cells, Sequencing techniques, Animal Cells, Immune Physiology, Medicine and Health Sciences, Macrophage, lcsh:Science, Immune Response, Innate Immune System, Multidisciplinary, RNA sequencing, Cell biology, Cytokine, Hyaluronan Receptors, Oncology, Cell Processes, Cytokines, Antibody, Cellular Types, Research Article, Immune Cells, Immunology, Biology, Antibodies, Monoclonal, Humanized, 03 medical and health sciences, Immune system, Extraction techniques, Antigen, Phagocytosis, Cell Line, Tumor, medicine, Animals, Humans, Blood Cells, Macrophages, CD44, lcsh:R, Lymphokine, Cancer, Biology and Life Sciences, Neoplasms, Experimental, Cell Biology, Molecular Development, medicine.disease, RNA extraction, Research and analysis methods, 030104 developmental biology, Molecular biology techniques, Immune System, biology.protein, lcsh:Q, Developmental Biology
Abstract

CD44, a transmembrane receptor reported to be involved in various cellular functions, is overexpressed in several cancer types and supposed to be involved in the initiation, progression and prognosis of these cancers. Since the sequence of events following the blockage of the CD44-HA interaction has not yet been studied in detail, we profiled xenograft tumors by RNA Sequencing to elucidate the mode of action of the anti-CD44 antibody RG7356. Analysis of tumor and host gene-expression profiles led us to the hypothesis that treatment with RG7356 antibody leads to an activation of the immune system. Using cytokine measurements we further show that this activation involves the secretion of chemo-attractants necessary for the recruitment of immune cells (i.e. macrophages) to the tumor site. We finally provide evidence for antibody-dependent cellular phagocytosis (ADCP) of the malignant cells by macrophages.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results