1. In vitro evaluations on canine monocyte-derived dendritic cells of a nanoparticles delivery system for vaccine antigen against Echinococcus granulosus
- Author
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Eyad Almouazen, Samira Azzouz-Maache, Nadège Milhau, Anne-Françoise Pétavy, T. Marchal, Imène Hellel-Bourtal, Sylvie E Bouteille, and Uruguaysito Benavides
- Subjects
0301 basic medicine ,CD4-Positive T-Lymphocytes ,Protozoan Vaccines ,medicine.medical_treatment ,Flatworms ,Monophosphoryl Lipid A ,02 engineering and technology ,Tropomyosin ,Lymphocyte Activation ,Monocytes ,Major Histocompatibility Complex ,Immunogenicity, Vaccine ,Antigen Encapsulation ,Animal Cells ,Medicine and Health Sciences ,Nanotechnology ,Public and Occupational Health ,Drug Delivery System Preparation ,Echinococcus granulosus ,Cells, Cultured ,Mammals ,Drug Carriers ,Vaccines, Synthetic ,Vaccines ,Multidisciplinary ,biology ,Chemistry ,Pharmaceutics ,Eukaryota ,Cell Differentiation ,021001 nanoscience & nanotechnology ,Vaccination and Immunization ,Lipid A ,Infectious Diseases ,Vertebrates ,Engineering and Technology ,Medicine ,Cellular Types ,0210 nano-technology ,Adjuvant ,Research Article ,Infectious Disease Control ,Polyesters ,Immune Cells ,Science ,Primary Cell Culture ,Immunology ,Antigen-Presenting Cells ,Antigens, Protozoan ,Major histocompatibility complex ,03 medical and health sciences ,Dogs ,Antigen ,Adjuvants, Immunologic ,Echinococcosis ,Helminths ,Vaccine Development ,parasitic diseases ,medicine ,Toxicity Tests, Acute ,Animals ,Cell Proliferation ,CD86 ,CD40 ,Pharmaceutical Processing Technology ,Cestodes ,Organisms ,Biology and Life Sciences ,Dendritic Cells ,Cell Biology ,biology.organism_classification ,Molecular biology ,Invertebrates ,030104 developmental biology ,Amniotes ,biology.protein ,Nanoparticles ,Clinical Immunology ,Preventive Medicine ,Clinical Medicine ,CD80 - Abstract
Since dogs play a central role in the contamination of humans and livestock with Echinococcus granulosus, the development of an effective vaccine for dogs is essential to control the disease caused by this parasite. For this purpose, a formulation based on biodegradable polymeric nanoparticles (NPs) as delivery system of recombinant Echinococcus granulosus antigen (tropomyosin EgTrp) adjuved with monophosphoryl lipid A (MPLA) has been developed. The obtained nanoparticles had a size of approximately 200 nm in diameter into which the antigen was correctly preserved and encapsulated. The efficiency of this system to deliver the antigen was evaluated in vitro on canine monocyte-derived dendritic cells (cMoDCs) generated from peripheral blood monocytes. After 48 h of contact between the formulations and cMoDCs, we observed no toxic effect on the cells but a strong internalization of the NPs, probably through different pathways depending on the presence or not of MPLA. An evaluation of cMoDCs activation by flow cytometry showed a stronger expression of CD80, CD86, CD40 and MHCII by cells treated with any of the tested formulations or with LPS (positive control) in comparison to cells treated with PBS (negative control). A higher activation was observed for cells challenged with EgTrp-NPs-MPLA compared to EgTrp alone. Formulations with MPLA, even at low ratio of MPLA, give better results than formulations without MPLA, proving the importance of the adjuvant in the nanoparticles structure. Moreover, autologous T CD4+ cell proliferation observed in presence of cMoDCs challenged with EgTrp-NPs-MPLA was higher than those observed after challenged with EgTrp alone (p
- Published
- 2020