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15 results on '"Camussi, Giovanni"'

1. Extracellular vesicles from human plasma for biomarkers discovery: Impact of anticoagulants and isolation techniques

Author

Bettio, Valentina, primary, Mazzucco, Eleonora, additional, Antona, Annamaria, additional, Cracas, Silvia, additional, Varalda, Marco, additional, Venetucci, Jacopo, additional, Bruno, Stefania, additional, Chiabotto, Giulia, additional, Venegoni, Chiara, additional, Vasile, Alessandra, additional, Chiocchetti, Annalisa, additional, Quaglia, Marco, additional, Camussi, Giovanni, additional, Cantaluppi, Vincenzo, additional, Panella, Massimiliano, additional, Rolla, Roberta, additional, Manfredi, Marcello, additional, and Capello, Daniela, additional

Published
2023
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2. Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in FSGS

Author

Doublier, Sophie, primary, Zennaro, Cristina, additional, Musante, Luca, additional, Spatola, Tiziana, additional, Candiano, Giovanni, additional, Bruschi, Maurizio, additional, Besso, Luca, additional, Cedrino, Massimo, additional, Carraro, Michele, additional, Ghiggeri, Gian Marco, additional, Camussi, Giovanni, additional, and Lupia, Enrico, additional

Published
2017
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3. Stem Cell-Derived, microRNA-Carrying Extracellular Vesicles: A Novel Approach to Interfering with Mesangial Cell Collagen Production in a Hyperglycaemic Setting

Author

Gallo, Sara, primary, Gili, Maddalena, additional, Lombardo, Giusy, additional, Rossetti, Alberto, additional, Rosso, Arturo, additional, Dentelli, Patrizia, additional, Togliatto, Gabriele, additional, Deregibus, Maria Chiara, additional, Taverna, Daniela, additional, Camussi, Giovanni, additional, and Brizzi, Maria Felice, additional

Published
2016
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5. Plasma concentration of platelet-derived microparticles is related to painful vaso-occlusive phenotype severity in sickle cell anemia

Author

Nebor, Danitza, Bowers, Andre, Connes, Philippe, Hardy-Dessources, Marie-Dominique, Knight-Madden, Jennifer, Cumming, Vanessa, Reid, Marvin, Romana, Marc, Camussi, Giovanni, Protéines de la membrane érythrocytaire et homologues non-érythroides, Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Basic Medical Sciences (Physiology Section), Faculty of Medical Sciences, Adaptations au Climat Tropical, Exercice et Santé (ACTES), Université des Antilles et de la Guyane (UAG), Université des Antilles (Pôle Guadeloupe), Université des Antilles (UA), Sickle Cell Unit, Tropical Medicine Research Institute, and Università degli studi di Torino (UNITO)

Subjects
Male, Erythrocytes, Epidemiology, [SDV]Life Sciences [q-bio], lcsh:Medicine, Gastroenterology, Severity of Illness Index, Biochemistry, Plasma Cell Disorders, Cell-Derived Microparticles, Blood plasma, Platelet, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Multidisciplinary, medicine.diagnostic_test, Hematology, Middle Aged, Flow Cytometry, Sickle cell anemia, Phenotype, Biomarker (medicine), Medicine, Female, Research Article, Adult, Blood Platelets, Platelets, medicine.medical_specialty, congenital, hereditary, and neonatal diseases and abnormalities, Adolescent, Clinical Research Design, Pain, macromolecular substances, Anemia, Sickle Cell, Flow cytometry, Internal medicine, Severity of illness, medicine, Humans, Particle Size, Biology, Sickle Cell Disease, Plasma Proteins, business.industry, lcsh:R, Proteins, medicine.disease, Hemoglobinopathies, Biomarker Epidemiology, Immunology, lcsh:Q, Hemoglobin, business, Biomarkers
Abstract

High plasma level of microparticles (MPs) deriving mainly from erythrocytes and platelets has been detected in sickle cell anemia (SCA) patients. Flow cytometry was used to determine the concentration of MPs in two groups of SCA patients exhibiting marked differences in painful vaso-occlusive crisis rates [a non-severe group (n = 17) and a severe group (n = 12)], and in a control group composed of healthy subjects (n = 20). A 3- to 4-fold increase of total MP plasma concentration was detected in SCA patients. Higher platelet-derived MPs concentration was detected in the severe SCA group while erythrocyte-derived MPs concentration was increased in the non-severe SCA patient group only. Our results suggest that plasma concentration of MPs shed by platelets is a biomarker of the vaso-occlusive phenotype-related severity.

Published
2013
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6. Neutrophil Gelatinase Associated Lipocalin Is an Early and Accurate Biomarker of Graft Function and Tissue Regeneration in Kidney Transplantation from Extended Criteria Donors

Author

Cantaluppi, Vincenzo, primary, Dellepiane, Sergio, additional, Tamagnone, Michela, additional, Medica, Davide, additional, Figliolini, Federico, additional, Messina, Maria, additional, Manzione, Ana Maria, additional, Gai, Massimo, additional, Tognarelli, Giuliana, additional, Ranghino, Andrea, additional, Dolla, Caterina, additional, Ferrario, Silvia, additional, Tetta, Ciro, additional, Segoloni, Giuseppe Paolo, additional, Camussi, Giovanni, additional, and Biancone, Luigi, additional

Published
2015
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9. Isolation, Characterization and Potential Role in Beta Cell-Endothelium Cross-Talk of Extracellular Vesicles Released from Human Pancreatic Islets

Author

Figliolini, Federico, primary, Cantaluppi, Vincenzo, additional, De Lena, Michela, additional, Beltramo, Silvia, additional, Romagnoli, Renato, additional, Salizzoni, Mauro, additional, Melzi, Raffaella, additional, Nano, Rita, additional, Piemonti, Lorenzo, additional, Tetta, Ciro, additional, Biancone, Luigi, additional, and Camussi, Giovanni, additional

Published
2014
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13. Urinary CD133+ Extracellular Vesicles Are Decreased in Kidney Transplanted Patients with Slow Graft Function and Vascular Damage.

Author

Dimuccio, Veronica, Ranghino, Andrea, Praticò Barbato, Loredana, Fop, Fabrizio, Biancone, Luigi, Camussi, Giovanni, and Bussolati, Benedetta

Subjects
CD antigens, VESICLES (Cytology), KIDNEY transplantation, BLOOD vessels, URINALYSIS, NEPHRONS
Abstract

Extracellular vesicles (EVs) present in the urine are mainly released from cells of the nephron and can therefore provide information on kidney function. We here evaluated the presence of vesicles expressing the progenitor marker CD133 in the urine of normal subjects and of patients undergoing renal transplant. We found that EV expressing CD133 were present in the urine of normal subjects, but not of patients with end stage renal disease. The first day after transplant, urinary CD133+ EVs were present at low levels, to increase thereafter (at day 7). Urinary CD133+ EVs significantly increased in patients with slow graft function in respect to those with early graft function. In patients with a severe pre-transplant vascular damage of the graft, CD133+ EVs did not increase at day 7. At variance, the levels of EVs expressing the renal exosomal marker CD24 did not vary in the urine of patients with end stage renal disease or in transplanted patients in respect to controls. Sorted CD133+ EVs were found to express glomerular and proximal tubular markers. These data indicate that urinary CD133+ EVs are continuously released during the homeostatic turnover of the nephron and may provide information on its function or regenerative potential. [ABSTRACT FROM AUTHOR]

Published
2014
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14. Bone Marrow-Derived Mesenchymal Stem Cells Repaired but Did Not Prevent Gentamicin-Induced Acute Kidney Injury through Paracrine Effects in Rats.

Author

Reis, Luciana A., Borges, Fernanda T., Simões, Manuel J., Borges, Andrea A., Sinigaglia-Coimbra, Rita, Schor, Nestor, and Camussi, Giovanni

Subjects
BONE marrow, MESENCHYMAL stem cells, MULTIPOTENT stem cells, GENTAMICIN, ACUTE kidney failure, KIDNEY injuries, PARACRINE mechanisms
Abstract

This study evaluated the effects of bone marrow-derived mesenchymal stem cells (BMSCs) or their conditioned medium (CM) on the repair and prevention of Acute Kidney Injury (AKI) induced by gentamicin (G). Animals received daily injections of G up to 20 days. On the 10th day, injections of BMSCs, CM, CM+trypsin, CM+RNase or exosome-like microvesicles extracted from the CM were administered. In the prevention groups, the animals received the BMSCs 24 h before or on the 5th day of G treatment. Creatinine (Cr), urea (U), FENa and cytokines were quantified. The kidneys were evaluated using hematoxylin/eosin staining and immunohystochemistry. The levels of Cr, U and FENa increased during all the periods of G treatment. The BMSC transplantation, its CM or exosome injections inhibited the increase in Cr, U, FENa, necrosis, apoptosis and also increased cell proliferation. The pro-inflammatory cytokines decreased while the anti-inflammatory cytokines increased compared to G. When the CM or its exosomes were incubated with RNase (but not trypsin), these effects were blunted. The Y chromosome was not observed in the 24-h prevention group, but it persisted in the kidney for all of the periods analyzed, suggesting that the injury is necessary for the docking and maintenance of BMSCs in the kidney. In conclusion, the BMSCs and CM minimized the G-induced renal damage through paracrine effects, most likely through the RNA carried by the exosome-like microvesicles. The use of the CM from BMSCs can be a potential therapeutic tool for this type of nephrotoxicity, allowing for the avoidance of cell transplantations. [ABSTRACT FROM AUTHOR]

Published
2012
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15. Isolation, characterization and potential role in beta cell-endothelium cross-talk of extracellular vesicles released from human pancreatic islets

Author

Vincenzo Cantaluppi, Giovanni Camussi, Luigi Biancone, Ciro Tetta, Renato Romagnoli, Michela De Lena, Silvia Beltramo, Raffaella Melzi, Lorenzo Piemonti, Rita Nano, Mauro Salizzoni, Federico Figliolini, Figliolini, Federico, Cantaluppi, Vincenzo, De Lena, Michela, Beltramo, Silvia, Romagnoli, Renato, Salizzoni, Mauro, Melzi, Raffaella, Nano, Rita, Piemonti, Lorenzo, Tetta, Ciro, Biancone, Luigi, Camussi, Giovanni, and Pathology/molecular and cellular medicine

Subjects
Angiogenesis, Physiology, Cell Communication, Insulin-Secreting Cells, Medicine and Health Sciences, Endothelial Cell, Multidisciplinary, geography.geographical_feature_category, Reverse Transcriptase Polymerase Chain Reaction, Research Support, Non-U.S. Gov't, Medicine (all), MicroRNA, Islet, Flow Cytometry, Blot, Vascular endothelial growth factor A, medicine.anatomical_structure, Transport Vesicle, Medicine, Beta cell, Anatomy, extracellular vesicles, Research Article, Human, Cell signaling, Cell Physiology, endocrine system, Science, Blotting, Western, Angiogenesis Inducing Agent, Endocrine System, Biology, Cellule beta pancreatiche, medicine, Journal Article, Humans, RNA, Messenger, Transport Vesicles, Pancreas, geography, Biochemistry, Genetics and Molecular Biology (all), Pancreatic islets, Biology and Life Sciences, Endothelial Cells, Cell Biology, Receptor Cross-Talk, Molecular biology, Transplantation, MicroRNAs, Agricultural and Biological Sciences (all), Insulin-Secreting Cell, Angiogenesis Inducing Agents, Developmental Biology
Abstract

The cross-talk between beta cells and endothelium plays a key role in islet physiopathology and in the revascularization process after islet transplantation. However, the molecular mechanisms involved in this cross-talk are not fully elucidated. Extracellular vesicles (EVs) are secreted membrane nanoparticles involved in inter-cellular communication through the transfer of proteins and nucleic acids. The aims of this study were: 1) isolation and characterization of EVs from human islets; 2) evaluation of the pro-angiogenic effect of islet-derived EVs on human islet endothelial cells (IECs). EVs were isolated by ultracentrifugation from conditioned medium of human islets and characterized by nanotrack analysis (Nanosight), FACS, western blot, bioanalyzer, mRNA/microRNA RT-PCR array. On IECs, we evaluated EV-induced insulin mRNA transfer, proliferation, resistance to apoptosis, in vitro angiogenesis, migration, gene and protein profiling. EVs sized 236±54 nm, expressed different surface molecules and islet-specific proteins (insulin, C-peptide, GLP1R) and carried several mRNAs (VEGFa, eNOS) and microRNAs (miR-27b, miR-126, miR-130 and miR-296) involved in beta cell function, insulin secretion and angiogenesis. Purified EVs were internalized into IECs inducing insulin mRNA expression, protection from apoptosis and enhancement of angiogenesis. Human islets release biologically active EVs able to shuttle specific mRNAs and microRNAs (miRNAs) into target endothelial cells. These results suggest a putative role for islet-derived EVs in beta cell-endothelium cross-talk and in the neoangiogenesis process which is critical for engraftment of transplanted islets. © 2014 Figliolini et al.

Published
2014

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