1. Fine mapping and candidate gene analysis of qTAC8, a major quantitative trait locus controlling tiller angle in rice (Oryza sativa L.)
- Author
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Fenglin Huang, Zhonghua Sheng, Xiangjin Wei, Peisong Hu, Gaoneng Shao, Shaoqing Tang, and Jiwai He
- Subjects
0106 biological sciences ,0301 basic medicine ,Candidate gene ,lcsh:Medicine ,Gene Expression ,Gene Sequencing ,Tiller (botany) ,Artificial Gene Amplification and Extension ,01 natural sciences ,Biochemistry ,Polymerase Chain Reaction ,Sequencing techniques ,Mobile Genetic Elements ,DNA sequencing ,lcsh:Science ,Genetics ,education.field_of_study ,Multidisciplinary ,food and beverages ,Chromosome Mapping ,Genomics ,Plants ,Crop Production ,Retrotransposons ,Experimental Organism Systems ,Candidate Gene Analysis ,Research Article ,Population ,Quantitative Trait Loci ,Biology ,Quantitative trait locus ,Oryza ,Genes, Plant ,Real-Time Polymerase Chain Reaction ,Research and Analysis Methods ,03 medical and health sciences ,Open Reading Frames ,Genetic Elements ,Plant and Algal Models ,DNA-binding proteins ,Gene Regulation ,Plant breeding ,Grasses ,education ,Molecular Biology Techniques ,Gene Prediction ,Molecular Biology ,Oryza sativa ,lcsh:R ,fungi ,Organisms ,Transposable Elements ,Biology and Life Sciences ,Proteins ,Computational Biology ,biology.organism_classification ,Genome Analysis ,Regulatory Proteins ,Plant Breeding ,030104 developmental biology ,Agronomy ,Genetic Loci ,lcsh:Q ,Rice ,010606 plant biology & botany ,Transcription Factors - Abstract
Rice tiller angle is an important agronomic trait that contributes to crop production and plays a vital role in high yield breeding. In this study, a recombinant inbred line (RIL) population derived from the cross of a glabrous tropical japonica rice D50 and an indica rice HB277, was used to investigate quantitative trait loci (QTLs) controlling rice tiller angle. Two major QTLs, qTAC8 and qTAC9, were detected. While qTAC9 mapped with a previously identified gene (TAC1), using a BC2F2 population qTAC8 was mapped to a 16.5 cM region between markers RM7049 and RM23175. Position of qTAC8 was narrowed to a 92 kb DNA region by two genetic segregating populations. Finally, one opening reading frame (ORF) was regarded as a candidate gene according to genomic sequencing and qRT-PCR analysis. In addition, a set of four near isogenic lines (NILs) were created to investigate the genetic relationship between those two QTLs, and one line carrying qTAC8 and qTAC9 presented additive effect of tiller angle, suggesting that these QTLs are involved in different genetic pathways. Our results provide a foundation for the cloning of qTAC8 and genetic improvement of the rice plant architecture.
- Published
- 2017