8 results on '"Chengchao Zheng"'
Search Results
2. Overexpression of Mitochondrial Phosphate Transporter 3 Severely Hampers Plant Development through Regulating Mitochondrial Function in Arabidopsis.
- Author
-
Fengjuan Jia, Xiaomin Wan, Wei Zhu, Dan Sun, Chengchao Zheng, Pei Liu, and Jinguang Huang
- Subjects
Medicine ,Science - Abstract
Mitochondria are abundant and important organelles present in nearly all eukaryotic cells, which maintain metabolic communication with the cytosol through mitochondrial carriers. The mitochondrial membrane localized phosphate transporter (MPT) plays vital roles in diverse development and signaling processes, especially the ATP biosynthesis. Among the three MPT genes in Arabidopsis genome, AtMPT3 was proven to be a major member, and its overexpression gave rise to multiple developmental defects including curly leaves with deep color, dwarfed stature, and reduced fertility. Transcript profiles revealed that genes involved in plant metabolism, cellular redox homeostasis, alternative respiration pathway, and leaf and flower development were obviously altered in AtMPT3 overexpression (OEMPT3) plants. Moreover, OEMPT3 plants also accumulated higher ATP content, faster respiration rate and more reactive oxygen species (ROS) than wild type plants. Overall, our studies showed that AtMPT3 was indispensable for Arabidopsis normal growth and development, and provided new sights to investigate its possible regulation mechanisms.
- Published
- 2015
- Full Text
- View/download PDF
3. Genome-wide comparative in silico analysis of the RNA helicase gene family in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza sativa.
- Author
-
Ruirui Xu, Shizhong Zhang, Jinguang Huang, and Chengchao Zheng
- Subjects
Medicine ,Science - Abstract
RNA helicases are enzymes that are thought to unwind double-stranded RNA molecules in an energy-dependent fashion through the hydrolysis of NTP. RNA helicases are associated with all processes involving RNA molecules, including nuclear transcription, editing, splicing, ribosome biogenesis, RNA export, and organelle gene expression. The involvement of RNA helicase in response to stress and in plant growth and development has been reported previously. While their importance in Arabidopsis and Oryza sativa has been partially studied, the function of RNA helicase proteins is poorly understood in Zea mays and Glycine max. In this study, we identified a total of RNA helicase genes in Arabidopsis and other crop species genome by genome-wide comparative in silico analysis. We classified the RNA helicase genes into three subfamilies according to the structural features of the motif II region, such as DEAD-box, DEAH-box and DExD/H-box, and different species showed different patterns of alternative splicing. Secondly, chromosome location analysis showed that the RNA helicase protein genes were distributed across all chromosomes with different densities in the four species. Thirdly, phylogenetic tree analyses identified the relevant homologs of DEAD-box, DEAH-box and DExD/H-box RNA helicase proteins in each of the four species. Fourthly, microarray expression data showed that many of these predicted RNA helicase genes were expressed in different developmental stages and different tissues under normal growth conditions. Finally, real-time quantitative PCR analysis showed that the expression levels of 10 genes in Arabidopsis and 13 genes in Zea mays were in close agreement with the microarray expression data. To our knowledge, this is the first report of a comparative genome-wide analysis of the RNA helicase gene family in Arabidopsis, Oryza sativa, Zea mays and Glycine max. This study provides valuable information for understanding the classification and putative functions of the RNA helicase gene family in crop growth and development.
- Published
- 2013
- Full Text
- View/download PDF
4. The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana.
- Author
-
Wei Zhu, Qing Miao, Dan Sun, Guodong Yang, Changai Wu, Jinguang Huang, and Chengchao Zheng
- Subjects
Medicine ,Science - Abstract
The mitochondrial phosphate transporter (MPT) plays crucial roles in ATP production in plant cells. Three MPT genes have been identified in Arabidopsis thaliana. Here we report that the mRNA accumulations of AtMPTs were up-regulated by high salinity stress in A. thaliana seedlings. And the transgenic lines overexpressing AtMPTs displayed increased sensitivity to salt stress compared with the wild-type plants during seed germination and seedling establishment stages. ATP content and energy charge was higher in overexpressing plants than those in wild-type A. thaliana under salt stress. Accordingly, the salt-sensitive phenotype of overexpressing plants was recovered after the exogenous application of atractyloside due to the change of ATP content. Interestingly, Genevestigator survey and qRT-PCR analysis indicated a large number of genes, including those related to gibberellin synthesis could be regulated by the energy availability change under stress conditions in A. thaliana. Moreover, the exogenous application of uniconazole to overexpressing lines showed that gibberellin homeostasis was disturbed in the overexpressors. Our studies reveal a possible link between the ATP content mediated by AtMPTs and gibberellin metabolism in responses to high salinity stress in A. thaliana.
- Published
- 2012
- Full Text
- View/download PDF
5. Genome-Wide Comparative In Silico Analysis of the RNA Helicase Gene Family in Zea mays and Glycine max: A Comparison with Arabidopsis and Oryza sativa
- Author
-
Chengchao Zheng, Jinguang Huang, Shizhong Zhang, and Ruirui Xu
- Subjects
Arabidopsis ,lcsh:Medicine ,Zea mays ,Gene Expression Regulation, Plant ,Transcription (biology) ,Gene expression ,lcsh:Science ,Phylogeny ,Genetics ,Multidisciplinary ,biology ,lcsh:R ,Intron ,Correction ,Helicase ,RNA ,food and beverages ,Oryza ,Genomics ,Non-coding RNA ,RNA Helicase A ,Organ Specificity ,RNA editing ,biology.protein ,lcsh:Q ,Soybeans ,RNA Helicases ,Research Article - Abstract
RNA helicases are enzymes that are thought to unwind double-stranded RNA molecules in an energy-dependent fashion through the hydrolysis of NTP. RNA helicases are associated with all processes involving RNA molecules, including nuclear transcription, editing, splicing, ribosome biogenesis, RNA export, and organelle gene expression. The involvement of RNA helicase in response to stress and in plant growth and development has been reported previously. While their importance in Arabidopsis and Oryza sativa has been partially studied, the function of RNA helicase proteins is poorly understood in Zea mays and Glycine max. In this study, we identified a total of RNA helicase genes in Arabidopsis and other crop species genome by genome-wide comparative in silico analysis. We classified the RNA helicase genes into three subfamilies according to the structural features of the motif II region, such as DEAD-box, DEAH-box and DExD/H-box, and different species showed different patterns of alternative splicing. Secondly, chromosome location analysis showed that the RNA helicase protein genes were distributed across all chromosomes with different densities in the four species. Thirdly, phylogenetic tree analyses identified the relevant homologs of DEAD-box, DEAH-box and DExD/H-box RNA helicase proteins in each of the four species. Fourthly, microarray expression data showed that many of these predicted RNA helicase genes were expressed in different developmental stages and different tissues under normal growth conditions. Finally, real-time quantitative PCR analysis showed that the expression levels of 10 genes in Arabidopsis and 13 genes in Zea mays were in close agreement with the microarray expression data. To our knowledge, this is the first report of a comparative genome-wide analysis of the RNA helicase gene family in Arabidopsis, Oryza sativa, Zea mays and Glycine max. This study provides valuable information for understanding the classification and putative functions of the RNA helicase gene family in crop growth and development.
- Published
- 2013
6. The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana
- Author
-
Qing Miao, Jinguang Huang, Dan Sun, Chengchao Zheng, Guodong Yang, Wei Zhu, and Changai Wu
- Subjects
Environmental Impacts ,Agricultural Biotechnology ,Arabidopsis Thaliana ,Arabidopsis ,lcsh:Medicine ,Plant Science ,Mitochondrion ,Sodium Chloride ,Stress Signaling Cascade ,chemistry.chemical_compound ,Adenosine Triphosphate ,Agricultural Production ,Model Organisms ,Gene Expression Regulation, Plant ,Plant and Algal Models ,Botany ,Molecular Cell Biology ,Arabidopsis thaliana ,Phosphate Transport Proteins ,Energy charge ,lcsh:Science ,Biology ,Cellular Stress Responses ,Multidisciplinary ,biology ,Arabidopsis Proteins ,fungi ,lcsh:R ,food and beverages ,Agriculture ,biology.organism_classification ,Plant cell ,Gibberellins ,Signaling Cascades ,Cell biology ,chemistry ,Seedling ,Gibberellin ,lcsh:Q ,Adenosine triphosphate ,Research Article ,Signal Transduction - Abstract
The mitochondrial phosphate transporter (MPT) plays crucial roles in ATP production in plant cells. Three MPT genes have been identified in Arabidopsis thaliana. Here we report that the mRNA accumulations of AtMPTs were up-regulated by high salinity stress in A. thaliana seedlings. And the transgenic lines overexpressing AtMPTs displayed increased sensitivity to salt stress compared with the wild-type plants during seed germination and seedling establishment stages. ATP content and energy charge was higher in overexpressing plants than those in wild-type A. thaliana under salt stress. Accordingly, the salt-sensitive phenotype of overexpressing plants was recovered after the exogenous application of atractyloside due to the change of ATP content. Interestingly, Genevestigator survey and qRT-PCR analysis indicated a large number of genes, including those related to gibberellin synthesis could be regulated by the energy availability change under stress conditions in A. thaliana. Moreover, the exogenous application of uniconazole to overexpressing lines showed that gibberellin homeostasis was disturbed in the overexpressors. Our studies reveal a possible link between the ATP content mediated by AtMPTs and gibberellin metabolism in responses to high salinity stress in A. thaliana.
- Published
- 2012
7. Overexpression of Mitochondrial Phosphate Transporter 3 Severely Hampers Plant Development through Regulating Mitochondrial Function in Arabidopsis
- Author
-
Jinguang Huang, Fengjuan Jia, Chengchao Zheng, Xiaomin Wan, Dan Sun, Pei Liu, and Wei Zhu
- Subjects
Alternative respiration ,Cellular respiration ,Blotting, Western ,Arabidopsis ,lcsh:Medicine ,Mitochondrion ,Real-Time Polymerase Chain Reaction ,Mitochondrial Membrane Transport Proteins ,Adenosine Triphosphate ,Gene Expression Regulation, Plant ,Botany ,Phosphate Transport Proteins ,Arabidopsis thaliana ,RNA, Messenger ,lcsh:Science ,Inner mitochondrial membrane ,Multidisciplinary ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,lcsh:R ,Wild type ,food and beverages ,Plants, Genetically Modified ,biology.organism_classification ,Mitochondria ,Cell biology ,Cytosol ,lcsh:Q ,Reactive Oxygen Species ,Research Article - Abstract
Mitochondria are abundant and important organelles present in nearly all eukaryotic cells, which maintain metabolic communication with the cytosol through mitochondrial carriers. The mitochondrial membrane localized phosphate transporter (MPT) plays vital roles in diverse development and signaling processes, especially the ATP biosynthesis. Among the three MPT genes in Arabidopsis genome, AtMPT3 was proven to be a major member, and its overexpression gave rise to multiple developmental defects including curly leaves with deep color, dwarfed stature, and reduced fertility. Transcript profiles revealed that genes involved in plant metabolism, cellular redox homeostasis, alternative respiration pathway, and leaf and flower development were obviously altered in AtMPT3 overexpression (OEMPT3) plants. Moreover, OEMPT3 plants also accumulated higher ATP content, faster respiration rate and more reactive oxygen species (ROS) than wild type plants. Overall, our studies showed that AtMPT3 was indispensable for Arabidopsis normal growth and development, and provided new sights to investigate its possible regulation mechanisms.
- Published
- 2015
8. NFYA1 Is Involved in Regulation of Postgermination Growth Arrest Under Salt Stress in Arabidopsis
- Author
-
Ya-Ru Fu, Jinguang Huang, Chengchao Zheng, Changai Wu, Yan-Jie Li, and Yi Fang
- Subjects
Arabidopsis ,Gene Expression ,lcsh:Medicine ,Plant Science ,Plant Genetics ,chemistry.chemical_compound ,Molecular cell biology ,DNA amplification ,Gene Expression Regulation, Plant ,Gene expression ,Arabidopsis thaliana ,lcsh:Science ,Abscisic acid ,Regulation of gene expression ,Multidisciplinary ,Ecology ,biology ,Salt Tolerance ,Signaling in Selected Disciplines ,Plants, Genetically Modified ,Phenotype ,Cell biology ,Nucleic acids ,Basic-Leucine Zipper Transcription Factors ,RNA Interference ,Signal transduction ,Signal Transduction ,Research Article ,Arabidopsis Thaliana ,Model Organisms ,Stress, Physiological ,Plant and Algal Models ,Plant Signaling ,Plant-Environment Interactions ,Botany ,Biology ,Transcription factor ,Arabidopsis Proteins ,Plant Ecology ,lcsh:R ,DNA ,biology.organism_classification ,Biosynthetic Pathways ,CCAAT-Binding Factor ,chemistry ,Mutation ,lcsh:Q ,Abscisic Acid ,Transcription Factors - Abstract
The nuclear factor Y (NF-Y), which is a ubiquitous transcription factor found in eukaryotes, is composed of three distinct subunits, namely, NF-YA, NF-YB, and NF-YC. Here, we firstly characterized the detailed function of the Arabidopsis NFYA1 factor. It is found that the 35S::AtNFYA1-overexpressed lines were hypersensitive to salt stress and Abscisic acid (ABA) during the early-postgermination growth stages. The transgenic lines exhibited a severe postgermination growth arrest compared with the wild-type (WT) under salt stress and ABA treatment. Interestingly, sodium tungstate, which is an ABA synthesis inhibitor, restored the salt-sensitive phenotype of the 35S::AtNFYA1 lines. Results of the qRT-PCR analysis showed that the mRNA levels of ABI3 and ABI5, as well as their downstream genes AtEM1 and AtEM6, were more greatly upregulated under salt stress during seed germination in the transgenic lines compared with those in WT. On the other hand, the NFYA1-RNAi lines were found to be insensitive to salt stress and exhibited decreased levels of ABI3, ABI5, EM1, and EM6 transcripts. Our results provide clear evidence supporting a role of AtNFYA1 in regulating postgermination growth arrest under salt stress.
- Published
- 2013
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