6 results on '"Fengxiang Zhang"'
Search Results
2. Genetic polymorphisms in ZFHX3 are associated with atrial fibrillation in a Chinese Han population.
- Author
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Yaowu Liu, Bixian Ni, Yuan Lin, Xin-guang Chen, Zhen Fang, Liyan Zhao, Zhibin Hu, and Fengxiang Zhang
- Subjects
Medicine ,Science - Abstract
The gene zinc finger homeobox 3 (ZFHX3) encodes a transcription factor with cardiac expression and its genetic variants are associated with atrial fibrillation (AF). We aimed to explore the associations between single nucleotide polymorphisms (SNPs) of ZFHX3 and the risk of AF in a Chinese Han population.We genotyped eight SNPs, including seven potentially functional SNPs and one previously reported SNP by using the middle-throughput iPLEX Sequenom MassARRAY platform. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated in logistic regression models.We enrolled a total of 1,593 Chinese Han origin individuals in the study, including 597 AF patients and 996 non-AF controls. Logistic regression analyses revealed that potentially functional SNPs rs6499600 and rs16971436 were associated with a decreased risk of AF (adjusted OR = 0.73, 95% CI: 0.63-0.86, P = 1.07×10-4; adjusted OR = 0.74, 95% CI: 0.56-0.98, P = 0.039, respectively). In addition, rs2106261 showed a robust association with an increased risk of AF (adjusted OR = 1.71, 95% CI: 1.46-2.00, P = 1.85×10-11). After multiple comparisons, rs16971436 conferred a borderline significant association with the risk of AF. Stratification analysis indicated that the risks of AF were statistically different among subgroups of age for rs2106261, and the effect for rs16971436 was more evident in subgroups of patients with coronary artery disease.In summary, our study investigated the role of genetic variants of ZFHX3 in AF and two SNPs (rs2106261, rs6499600) showed significant associations while rs16971436 conferred a borderline significant association with AF risk in Chinese Han populations. However, further large and functional studies are warranted to confirm our findings.
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- 2014
- Full Text
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3. Intramyocardial Injection of Pig Pluripotent Stem Cells Improves Left Ventricular Function and Perfusion: A Study in a Porcine Model of Acute Myocardial Infarction.
- Author
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Xiaorong Li, Fengxiang Zhang, Guixian Song, Weijuan Gu, Minglong Chen, Bing Yang, Dianfu Li, Daowu Wang, and Kejiang Cao
- Subjects
Medicine ,Science - Abstract
Induced pluripotent stem (iPS) cells have the potential to differentiate to various types of cardiovascular cells to repair an injured heart. The potential therapeutic benefits of iPS cell based treatment have been established in small-animal models of myocardial infarction (MI). We hypothesize that porcine iPS (piPS) cell transplantation may be an effective treatment for MI. After a 90-minute occlusion of the left anterior descending artery in a porcine model, undifferentiated piPS cells or PBS were injected into the ischemic myocardium. Cardiac function, myocardial perfusion and cell differentiation were investigated. One week after piPS cell delivery, global left ventricular ejection fraction (LVEF) significantly decreased in both the iPS group and the PBS group compared to the Sham group (p
- Published
- 2013
- Full Text
- View/download PDF
4. Intramyocardial transplantation of undifferentiated rat induced pluripotent stem cells causes tumorigenesis in the heart.
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Yuzhen Zhang, Dan Wang, Minglong Chen, Bing Yang, Fengxiang Zhang, and Kejiang Cao
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Medicine ,Science - Abstract
BACKGROUND: Induced pluripotent stem cells (iPSCs) are a novel candidate for use in cardiac stem cell therapy. However, their intrinsic tumorigenicity requires further investigation prior to use in a clinical setting. In this study we investigated whether undifferentiated iPSCs are tumorigenic after intramyocardial transplantation into immunocompetent allogeneic recipients. METHODOLOGY/PRINCIPAL FINDINGS: We transplanted 2 × 10(4), 2 × 10(5), or 2 × 10(6) cells from the established rat iPSC line M13 intramyocardially into intact or infarcted hearts of immunocompetent allogeneic rats. Transplant duration was 2, 4, or 6 weeks. Histological examination with hematoxylin-eosin staining confirmed that undifferentiated rat iPSCs could generate heterogeneous tumors in both intracardiac and extracardiac sites. Furthermore, tumor incidence was independent of cell dose, transplant duration, and the presence or absence of myocardial infarction. CONCLUSIONS/SIGNIFICANCE: Our study demonstrates that allogeneic iPSC transplantation in the heart will likely result in in situ tumorigenesis, and that cells leaked from the beating heart are a potential source of tumor spread, underscoring the importance of evaluating the safety of future iPSC therapy for cardiac disease.
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- 2011
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5. Hepatocyte Growth Factor Modification Enhances the Anti-Arrhythmic Properties of Human Bone Marrow-Derived Mesenchymal Stem Cells
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Lin-Lin Wang, Wei Du, Fengxiang Zhang, Xiaorong Li, Yi-Long Man, Weijuan Gu, Yi-Chao Yu, Jian Zhang, Weizhu Ju, Kejiang Cao, Chu-Tse Wu, Yan Chen, Hua Wang, and Zidun Wang
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Male ,Cell Transplantation ,Swine ,Myocardial Infarction ,lcsh:Medicine ,Infarction ,Apoptosis ,Sudden Cardiac Death ,Neovascularization ,Animal Cells ,Medicine and Health Sciences ,Cardiac Arrest ,Blood and Lymphatic System Procedures ,Myocardial infarction ,lcsh:Science ,Regulation of gene expression ,Multidisciplinary ,Hepatocyte Growth Factor ,Stem Cells ,Stem Cell Therapy ,Bioassays and Physiological Analysis ,cardiovascular system ,Hepatocyte growth factor ,medicine.symptom ,Cellular Types ,medicine.drug ,Research Article ,Cell Survival ,Heart Ventricles ,Cardiology ,Neovascularization, Physiologic ,Surgical and Invasive Medical Procedures ,Bone Marrow Cells ,Research and Analysis Methods ,Autonomic Nervous System ,Mesenchymal Stem Cell Transplantation ,medicine ,Anti arrhythmic ,Animals ,Humans ,cardiovascular diseases ,Clinical Genetics ,Transplantation ,business.industry ,lcsh:R ,Mesenchymal stem cell ,Electrophysiological Techniques ,Biology and Life Sciences ,Mesenchymal Stem Cells ,Arrhythmias, Cardiac ,Cell Biology ,medicine.disease ,Electric Stimulation ,Gene Expression Regulation ,Cancer research ,lcsh:Q ,Cardiac Pacing ,Cardiac Electrophysiology ,business ,Stem Cell Transplantation - Abstract
BACKGROUND/AIMS: Chronic myocardial infarction (MI) results in the formation of arrhythmogenic substrates, causing lethal ventricular arrhythmia (VA). We aimed to determine whether mesenchymal stem cells (MSCs) carrying a hepatocyte growth factor (HGF) gene modification (HGF-MSCs) decrease the levels of arrhythmogenic substrates and reduce the susceptibility to developing VA compared with unmodified MSCs and PBS in a swine infarction model. METHODS: The left descending anterior artery was balloon-occluded to establish an MI model. Four weeks later, the randomly grouped pigs were administered MSCs, PBS or HGF-MSCs via thoracotomy. After an additional four weeks, dynamic electrocardiography was performed to assess heart rate variability, and programmed electrical stimulation was conducted to evaluate the risk for VA. Then, the pigs were euthanized for morphometric, immunofluorescence and western blot analyses. RESULTS: The HGF-MSC group displayed the highest vessel density and Cx43 expression levels, and the lowest levels of apoptosis, and tyrosine hydroxylase (TH) and growth associated protein 43 (GAP43) expression. Moreover, the HGF-MSC group exhibited a decrease in the number of sympathetic nerve fibers, substantial decreases in the low frequency and the low-/high- frequency ratio and increases in the root mean square of successive differences (rMSSD) and the percentage of successive normal sinus R-R intervals longer than 50 ms (pNN50), compared with the other two groups. Finally, the HGF-MSC group displayed the lowest susceptibility to developing VA. CONCLUSION: HGF-MSCs displayed potent antiarrhythmic effects, reducing the risk for VA.
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- 2014
6. Intramyocardial Injection of Pig Pluripotent Stem Cells Improves Left Ventricular Function and Perfusion: A Study in a Porcine Model of Acute Myocardial Infarction
- Author
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Daowu Wang, Kejiang Cao, Bing Yang, Fengxiang Zhang, Xiaorong Li, Guixian Song, Dianfu Li, Weijuan Gu, and Minglong Chen
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Male ,Swine ,Cellular differentiation ,Myocardial Infarction ,lcsh:Medicine ,Cardiovascular ,Ventricular Function, Left ,Molecular Cell Biology ,Myocardial infarction ,Induced pluripotent stem cell ,lcsh:Science ,Multidisciplinary ,Ejection fraction ,Stem Cells ,Statistics ,Cell Differentiation ,Heart ,Animal Models ,Cardiovascular physiology ,medicine.anatomical_structure ,Acute Disease ,Cardiology ,cardiovascular system ,Medicine ,Female ,Cellular Types ,Perfusion ,Research Article ,Artery ,Cardiac function curve ,medicine.medical_specialty ,Histology ,Clinical Research Design ,Immunology ,Induced Pluripotent Stem Cells ,Biostatistics ,Immunocompromised Host ,Model Organisms ,Internal medicine ,medicine ,Animals ,Regeneration ,Animal Models of Disease ,Biology ,Tomography, Emission-Computed, Single-Photon ,Transplantation ,business.industry ,Myocardium ,lcsh:R ,Immunologic Subspecialties ,medicine.disease ,Actins ,Surgery ,Disease Models, Animal ,Clinical Immunology ,lcsh:Q ,business ,Mathematics ,Developmental Biology - Abstract
Induced pluripotent stem (iPS) cells have the potential to differentiate to various types of cardiovascular cells to repair an injured heart. The potential therapeutic benefits of iPS cell based treatment have been established in small-animal models of myocardial infarction (MI). We hypothesize that porcine iPS (piPS) cell transplantation may be an effective treatment for MI. After a 90-minute occlusion of the left anterior descending artery in a porcine model, undifferentiated piPS cells or PBS were injected into the ischemic myocardium. Cardiac function, myocardial perfusion and cell differentiation were investigated. One week after piPS cell delivery, global left ventricular ejection fraction (LVEF) significantly decreased in both the iPS group and the PBS group compared to the Sham group (p
- Published
- 2013
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