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Your search keyword '"Giuliano, Giovanni"' showing total 11 results
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11 results on '"Giuliano, Giovanni"'

3. Tomato R2R3-MYB proteins SlANT1 and SlAN2: Same protein activity, different roles

Author

Kiferle, Claudia, Fantini, Elio, Bassolino, Laura, Povero, Giovanni, Spelt, Cornelis, Buti, Sara, Giuliano, Giovanni, Quattrocchio, Francesca, Koes, Ronald, Perata, Pierdomenico, Gonzali, Silvia, Sub Plant Ecophysiology, Dep Biologie, Plant Ecophysiology, Sub Plant Ecophysiology, Dep Biologie, Plant Ecophysiology, and Plant Development & (Epi)Genetics (SILS, FNWI)

Subjects
Genotype, Light, Science, Regulator, lcsh:Medicine, R2R3-MYB, Genetically modified crops, Biology, tomato, Real-Time Polymerase Chain Reaction, Biochemistry, Solanum lycopersicum, Gene Expression Regulation, Plant, Gene expression, Botany, Arabidopsis thaliana, MYB, Genetically modified tomato, RNA, Messenger, Promoter Regions, Genetic, lcsh:Science, Gene, Transcription factor, Phylogeny, Plant Proteins, Medicine(all), Multidisciplinary, Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Reverse Transcriptase Polymerase Chain Reaction, lcsh:R, fungi, food and beverages, biology.organism_classification, Plants, Genetically Modified, anthocyanins, Cold Temperature, carbohydrates (lipids), Phenotype, Medicine, lcsh:Q, Research Article, Transcription Factors, Genetics and Molecular Biology(all)
Abstract

Anthocyanins are water-soluble polyphenolic compounds with a high nutraceutical value. Despite the fact that cultivated tomato varieties do not accumulate anthocyanins in the fruit, the biosynthetic pathway can be activated in the vegetative organs by several environmental stimuli. Little is known about the molecular mechanisms regulating anthocyanin synthesis in tomato. Here, we carried out a molecular and functional characterization of two genes, SlAN2 and SlANT1, encoding two R2R3-MYB transcription factors. We show that both can induce ectopic anthocyanin synthesis in transgenic tomato lines, including the fruit. However, only SlAN2 acts as a positive regulator of anthocyanin synthesis in vegetative tissues under high light or low temperature conditions.

Published
2015

7. First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach

Author

Vitulo, Nicola, primary, Albiero, Alessandro, additional, Forcato, Claudio, additional, Campagna, Davide, additional, Dal Pero, Francesca, additional, Bagnaresi, Paolo, additional, Colaiacovo, Moreno, additional, Faccioli, Primetta, additional, Lamontanara, Antonella, additional, Šimková, Hana, additional, Kubaláková, Marie, additional, Perrotta, Gaetano, additional, Facella, Paolo, additional, Lopez, Loredana, additional, Pietrella, Marco, additional, Gianese, Giulio, additional, Doležel, Jaroslav, additional, Giuliano, Giovanni, additional, Cattivelli, Luigi, additional, Valle, Giorgio, additional, and Stanca, A. Michele, additional

Published
2011
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11. A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection.

Author

Demurtas, Olivia C., Massa, Silvia, Ferrante, Paola, Venuti, Aldo, Franconi, Rosella, and Giuliano, Giovanni

Subjects
CHLAMYDOMONAS, PAPILLOMAVIRUSES, VIRAL vaccines, VIRAL proteins, CANCER cells, VIRAL antigens, GENE expression in viruses
Abstract

Background: The E7 protein of the Human Papillomavirus (HPV) type 16, being involved in malignant cellular transformation, represents a key antigen for developing therapeutic vaccines against HPV-related lesions and cancers. Recombinant production of this vaccine antigen in an active form and in compliance with good manufacturing practices (GMP) plays a crucial role for developing effective vaccines. E7-based therapeutic vaccines produced in plants have been shown to be active in tumor regression and protection in pre-clinical models. However, some drawbacks of in whole-plant vaccine production encouraged us to explore the production of the E7-based therapeutic vaccine in Chlamydomonas reinhardtii, an organism easy to grow and transform and fully amenable to GMP guidelines. Methodology/Principal Findings: An expression cassette encoding E7GGG, a mutated, attenuated form of the E7 oncoprotein, alone or as a fusion with affinity tags (His6 or FLAG), under the control of the C. reinhardtii chloroplast psbD 5′ UTR and the psbA 3′ UTR, was introduced into the C. reinhardtii chloroplast genome by homologous recombination. The protein was mostly soluble and reached 0.12% of total soluble proteins. Affinity purification was optimized and performed for both tagged forms. Induction of specific anti-E7 IgGs and E7-specific T-cell proliferation were detected in C57BL/6 mice vaccinated with total Chlamydomonas extract and with affinity-purified protein. High levels of tumor protection were achieved after challenge with a tumor cell line expressing the E7 protein. Conclusions: The C. reinhardtii chloroplast is a suitable expression system for the production of the E7GGG protein, in a soluble, immunogenic form. The production in contained and sterile conditions highlights the potential of microalgae as alternative platforms for the production of vaccines for human uses. [ABSTRACT FROM AUTHOR]

Published
2013
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