1. Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells
- Author
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Hildenbrand, Georg, Metzler, Philipp, Pilarczyk, Götz, Bobu, Vladimir, Kriz, Wilhelm, Hosser, Hiltraud, Fleckenstein, Jens, Krufczik, Matthias, Bestvater, Felix, Wenz, Frederik, and Hausmann, Michael
- Subjects
Cytoplasm ,Cell Enumeration Techniques ,Metal Nanoparticles ,lcsh:Medicine ,Breast Neoplasms ,Research and Analysis Methods ,Endoplasmic Reticulum ,Cell Line, Tumor ,Nanotechnology ,Humans ,Electron Microscopy ,RNA, Neoplasm ,lcsh:Science ,Cell Nucleus ,Microscopy ,Secretory Pathway ,Physics ,lcsh:R ,Magnetism ,Biology and Life Sciences ,Cell Biology ,Condensed Matter Physics ,Probability Theory ,Magnetic Fields ,Cell Processes ,Physical Sciences ,Engineering and Technology ,Nanoparticles ,Transmission Electron Microscopy ,lcsh:Q ,Gold ,Cellular Structures and Organelles ,Mathematics ,Research Article ,Statistical Distributions - Abstract
Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles ("SmartFlares") can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both-SmartFlares and unmodified GNPs-lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles.
- Published
- 2018