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Your search keyword '"Huang, Shu"' showing total 45 results
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45 results on '"Huang, Shu"'

1. Design and characterization of hyperactive mutants of the Agrobacterium tumefaciens telomere resolvase, TelA.

Author

Huang, Shu Hui, Abrametz, Kayla, McGrath, Siobhan L., and Kobryn, Kerri

Subjects
*AGROBACTERIUM tumefaciens, *TELOMERES, *DNA structure, *CATALYTIC domains, *HAIRPIN (Genetics), *HOLLIDAY junctions
Abstract

Telomere resolvases are a family of DNA cleavage and rejoining enzymes that produce linear DNAs terminated by hairpin telomeres from replicated intermediates in bacteria that possess linear replicons. The telomere resolvase of Agrobacterium tumefaciens, TelA, has been examined at the structural and biochemical level. The N-terminal domain of TelA, while not required for telomere resolution, has been demonstrated to play an autoinhibitory role in telomere resolution, conferring divalent metal responsiveness on the reaction. The N-terminal domain also inhibits the competing reactions of hp telomere fusion and recombination between replicated telomere junctions. Due to the absence of the N-terminal domain from TelA/DNA co-crystal structures we produced an AlphaFold model of a TelA monomer. The AlphaFold model suggested the presence of two inhibitory interfaces; one between the N-terminal domain and the catalytic domain and a second interface between the C-terminal helix and the N-core domain of the protein. We produced mutant TelA's designed to weaken these putative interfaces to test the validity of the modeled interfaces. While our analysis did not bear out the details of the predicted interfaces the model was, nonetheless, extremely useful in guiding design of mutations that, when combined, demonstrated an additive activation of TelA exceeding 250-fold. For some of these hyperactive mutants stimulation of telomere resolution has also been accompanied by activation of competing reactions. However, we have also characterized hyperactive TelA mutants that retain enough autoinhibition to suppress the competing reactions. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Gender disparity between first and senior authors on liver cancer research in the top journals of Gastroenterology and Hepatology.

Author

Shi, Huiqin, Xu, Huan, Huang, Shu, Tan, Zhenju, Ma, Xinyue, Zhang, Han, Zhang, Wei, Shi, Lei, Zhong, Xiaolin, Lü, Muhan, Chen, Xia, and Tang, Xiaowei

Subjects
LIVER cancer, GENDER inequality, WOMEN authors, MALE authors, CANCER research, LIVER
Abstract

Background: Gender disparity is pervasive in academic medicine. This study aimed to assess the disparity between men and women with regard to first and senior author positions in primary studies on liver cancer over the last two decades. Methods: We conducted a review of articles published in high-impact factor journals of the field of Gastroenterology and Hepatology in 2005, 2010, 2015 and 2020. First and senior authors of all ages were considered as the study population. The authors' genders were determined using the online artificial intelligence tool genderize.io (https://genderize.io/). The disparity between men and women authors was assessed using the linear-by-linear association test. Results: 665 original articles from 10 journals were reviewed. The point prevalence of first women authors was 25.0% compared with 75.0% for men. The point prevalence of senior women authors was 16.3% compared with 83.7% for men. From 2000 to 2020, the proportion of first women authors increased 14.4% to 26.8% compared with 85.6%-73.2% for men (P = 0.009), and the proportion of senior women authors increased from 7.4% to 19.5%, compared with 92.6%-80.5% for men (P = 0.035). The factor independently associated with a reduced representation of women among first authors was the region of author. The factor independently associated with a reduced representation of women among senior authors was the impact factor of journals. Conclusion: The findings indicated a remarkable increase in the proportion of women, both first and senior authors, over the past two decades in the field of liver cancers. However, the representation of women authors in this area is far less than that of men. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor

Author

Wang, Bi-Juan, primary, Huang, Shih-Han, additional, Kao, Cheng-Li, additional, Muller, Christo J. F., additional, Wang, Ya-Pei, additional, Chang, Kai-Hsiung, additional, Wen, Hui-Chin, additional, Yeh, Chien-Chih, additional, Shih, Li-Jane, additional, Kao, Yung-Hsi, additional, Huang, Shu-Pin, additional, Li, Chia-Yang, additional, and Chuu, Chih-Pin, additional

Published
2022
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21. Prefrontal Lobe Brain Reserve Capacity with Resistance to Higher Global Amyloid Load and White Matter Hyperintensity Burden in Mild Stage Alzheimer’s Disease

Author

Chang, Ya-Ting, primary, Huang, Chi-Wei, additional, Chen, Nai-Ching, additional, Lin, Kun-Ju, additional, Huang, Shu-Hua, additional, Chang, Yen-Hsiang, additional, Hsu, Shih-Wei, additional, Chang, Wen-Neng, additional, Lui, Chun-Chung, additional, Hsu, Che-Wei, additional, and Chang, Chiung-Chih, additional

Published
2016
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23. Concurrent Preoperative Presence of Hydronephrosis and Flank Pain Independently Predicts Worse Outcome of Upper Tract Urothelial Carcinoma

Author

Yeh, Hsin-Chih, primary, Jan, Hau-Chern, additional, Wu, Wen-Jeng, additional, Li, Ching-Chia, additional, Li, Wei-Ming, additional, Ke, Hung-Lung, additional, Huang, Shu-Pin, additional, Liu, Chia-Chu, additional, Lee, Yung-Chin, additional, Yang, Sheau-Fang, additional, Liang, Peir-In, additional, and Huang, Chun-Nung, additional

Published
2015
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33. Correction: Uremic Pruritus, Dialysis Adequacy, and Metabolic Profiles in Hemodialysis Patients: A Prospective 5-Year Cohort Study

Author

Ko, Mei-Ju, primary, Wu, Hon-Yen, additional, Chen, Hung-Yuan, additional, Chiu, Yen-Ling, additional, Hsu, Shih-Ping, additional, Pai, Mei-Fen, additional, Yang, Chun-Fu, additional, Lu, Hui-Min, additional, Huang, Shu-Chen, additional, Yang, Shao-Yu, additional, Wen, Su-Ying, additional, Chiu, Hsien-Ching, additional, Hu, Fu-Chang, additional, Peng, Yu-Sen, additional, and Jee, Shiou-Hwa, additional

Published
2013
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44. Molecular Epidemiology and Antimicrobial Resistance of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolates in Taiwan, 2010.

Author

Chen, Chih-Jung, Huang, Yhu-Chering, Su, Lin-Hui, Wu, Tsu-Lan, Huang, Shu-Huan, Chien, Chun-Chih, Chen, Po-Yen, Lu, Min-Chi, and Ko, Wen-Chien

Subjects
MOLECULAR epidemiology, ANTI-infective agents, METHICILLIN-resistant staphylococcus aureus, HOSPITAL care, LACTAMS, MULTIVARIATE analysis
Abstract

The information of molecular characteristics and antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus (MRSA) is essential for control and treatment of diseases caused by this medically important pathogen. A total of 577 clinical MRSA bloodstream isolates from six major hospitals in Taiwan were determined for molecular types, carriage of Panton-Valentine leukocidin (PVL) and sasX genes and susceptibilities to 9 non-beta-lactam antimicrobial agents. A total of 17 genotypes were identified in 577 strains by pulsotyping. Five major pulsotypes, which included type A (26.2%, belonging to sequence type (ST) 239, carrying type III staphylococcal chromosomal cassette mec (SCCmec), type F (18.9%, ST5-SCCmecII), type C (18.5%, ST59-SCCmecIV), type B (12.0%, ST239-SCCmecIII) and type D (10.9%, ST59-SCCmecVT/IV), prevailed in each of the six sampled hospitals. PVL and sasX genes were respectively carried by ST59-type D strains and ST239 strains with high frequencies (93.7% and 99.1%, respectively) but rarely detected in strains of other genotypes. Isolates of different genotypes and from different hospitals exhibited distinct antibiograms. Multi-resistance to ≥3 non-beta-lactams was more common in ST239 isolates (100%) than in ST5 isolates (97.2%, P = 0.0347) and ST59 isolates (8.2%, P<0.0001). Multivariate analysis further indicated that the genotype, but not the hospital, was an independent factor associated with muti-resistance of the MRSA strains. In conclusion, five common MRSA clones with distinct antibiograms prevailed in the major hospitals in Taiwan in 2010. The antimicrobial susceptibility pattern of invasive MRSA was mainly determined by the clonal distribution. [ABSTRACT FROM AUTHOR]

Published
2014
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45. Uremic Pruritus, Dialysis Adequacy, and Metabolic Profiles in Hemodialysis Patients: A Prospective 5-Year Cohort Study.

Author

Ko, Mei-Ju, Wu, Hon-Yen, Chen, Hung-Yuan, Chiu, Yen-Ling, Hsu, Shih-Ping, Pai, Mei-Fen, Ju-YehYang, Lai, Chun-Fu, Lu, Hui-Min, Huang, Shu-Chen, Yang, Shao-Yu, Wen, Su-Yin, Chiu, Hsien-Ching, Hu, Fu-Chang, Peng, Yu-Sen, and Jee, Shiou-Hwa

Subjects
KIDNEY diseases, HEMODIALYSIS patients, COHORT analysis, METABOLIC disorders, REGRESSION analysis, DEMOGRAPHY, CLINICAL trials
Abstract

Background: Uremic pruritus is a common and intractable symptom in patients on chronic hemodialysis, but factors associated with the severity of pruritus remain unclear. This study aimed to explore the associations of metabolic factors and dialysis adequacy with the aggravation of pruritus. Methods: We conducted a 5-year prospective cohort study on patients with maintenance hemodialysis. A visual analogue scale (VAS) was used to assess the intensity of pruritus. Patient demographic and clinical characteristics, laboratory parameters, dialysis adequacy (assessed by Kt/V), and pruritus intensity were recorded at baseline and follow-up. Change score analysis of the difference score of VAS between baseline and follow-up was performed using multiple linear regression models. The optimal threshold of Kt/V, which is associated with the aggravation of uremic pruritus, was determined by generalized additive models and receiver operating characteristic analysis. Results: A total of 111 patients completed the study. Linear regression analysis showed that lower Kt/V and use of low-flux dialyzer were significantly associated with the aggravation of pruritus after adjusting for the baseline pruritus intensity and a variety of confounding factors. The optimal threshold value of Kt/V for pruritus was 1.5 suggested by both generalized additive models and receiver operating characteristic analysis. Conclusions: Hemodialysis with the target of Kt/V ≥1.5 and use of high-flux dialyzer may reduce the intensity of pruritus in patients on chronic hemodialysis. Further clinical trials are required to determine the optimal dialysis dose and regimen for uremic pruritus. [ABSTRACT FROM AUTHOR]

Published
2013
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