9 results on '"Huijie, Zhang"'
Search Results
2. Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells.
- Author
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Jinyu Li, Xiaoying Meng, Changzhi Wang, Huijie Zhang, Hening Chen, Peiying Deng, Juan Liu, Meiyier Huandike, Jie Wei, and Limin Chai
- Subjects
Medicine ,Science - Abstract
Coptidis alkaloids are the primary active components of Coptis chinensis Franch. Clinical and pharmacodynamic studies have confirmed that Coptidis alkaloids have multiple therapeutic effects including anti-inflammatory, antioxidant and antitumor effects, and they are usually used to treat various inflammatory disorders and related diseases. Mouse bone marrow cells (BMCs) were isolated from BALB/c mice. Immune-mediated destruction of BMCs was induced by interferon (IFN) -γ. High-performance liquid chromatography-electrospray ionization/ mass spectrometry was used to analyze the ingredients of the aqueous extract from Coptis chinensis Franch. The results confirmed that Coptidis alkaloids were the predominant ingredients in the aqueous extract from Coptis chinensis. The functional mechanism of Coptidis alkaloids in inhibiting immune-mediated destruction of BMCs was studied in vitro. After Coptidis alkaloid treatment, the percentages of apoptotic BMCs and the proliferation and differentiation of helper T (Th) cells and regulatory T (Treg) cells were measured by flow cytometry. The expression and distribution of T-bet in BMCs were observed by immunofluorescence. Western blotting analysis was used to assay the expression of key molecules in the Fas apoptosis and Jak/Stats signaling pathways in BMCs. We identified five alkaloids in the aqueous extract of Coptis chinensis. The apoptotic ratios of BMCs induced by IFN-γ were decreased significantly after Coptidis alkaloid treatment. The levels of key molecules (Fas, Caspase-3, cleaved Caspase-3, Caspase-8 and Caspase-8) in Fas apoptosis signaling pathways also decreased significantly after treatment with low concentrations of Coptidis alkaloids. Coptidis alkaloids were also found to inhibit the proliferation of Th1 and Th17 cells and induce the differentiation of Th2 and Treg cells; further, the distribution of T-bet in BMCs was decreased significantly. In addition, the levels of Stat-1, phospho-Stat-1 and phospho-Stat-3 were also reduced after Coptidis alkaloid treatment. These results indicate that Coptidis alkaloids extracted by water decoction from Coptis chinensis Franch could inhibit the proliferation and differentiation of T lymphocytes, attenuate the apoptosis of BMCs, and suppress the immune-mediated destruction of the BMCs induced by pro-inflammatory cytokines.
- Published
- 2020
- Full Text
- View/download PDF
3. Enhancing organic and inorganic carbon sequestration in calcareous soil by the combination of wheat straw and wood ash and/or lime.
- Author
-
Huili Zhao, Huijie Zhang, Abdul Ghaffar Shar, Jifei Liu, Yanlong Chen, Songjie Chu, and Xiaohong Tian
- Subjects
Medicine ,Science - Abstract
Increasing organic carbon sequestration in agricultural soils is important for improving soil fertility and mitigating climate change. Wood ash is generally applied as a potassium fertilizer, but the effects of simultaneous incorporation of wood ash and crop straw on the turnover of soil organic carbon (SOC) and inorganic carbon (SIC) are not well understood. In this study, a 118-day lab incubation experiment was conducted using a calcareous soil (with 10 years of continuous maize cropping history) to study the effects of adding wheat straw, wood ash and lime. Our study showed that straw addition led to an increase in both SOC (19%) and SIC (3%). Wood ash and lime addition decreased CO2 emission by 182 and 1210 mg kg-1 and increased SIC by 125 and 1001 mg kg-1 during the incubation, respectively, which was due to supply of CaO from wood ash and lime. The increase of SOC content was 2.4% due to the addition of lime. In addition to straw addition enhanced straw-derived OC content, the addition of lime also increased straw-derived OC content by 34.5%. This study demonstrated that lime was more effective in reducing CO2 emission and and enhancing SOC than wood ash. In conclusion, adding lime to calcareous soil might be an effective method of enhancing carbon sequestration and slowing climate change.
- Published
- 2018
- Full Text
- View/download PDF
4. Association of serum irisin with metabolic syndrome in obese Chinese adults.
- Author
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Bing Yan, Xiulin Shi, Huijie Zhang, Lingling Pan, Zhimin Ma, Suhuan Liu, Yongwen Liu, Xiaoying Li, Shuyu Yang, and Zhibin Li
- Subjects
Medicine ,Science - Abstract
Irisin, a recently identified novel myokine, drives brown-fat-like conversion of white adipose tissues and has been proposed to mediate beneficial effects of exercise on metabolism. Circulating irisin was significantly reduced in type 2 diabetes patients; however, no evidence is available about its association with metabolic syndrome (MetS) and effects of adiposity and muscle mass on circulating irisin have been controversial. Cross-sectional data on socio-demographic, lifestyle, clinical characteristics and serum irisin were collected for 1,115 community-living Chinese adults with central obesity. Associations of serum irisin with MetS (central obesity plus any two of the following four factors (raised blood pressure (BP), raised fasting plasma glucose (FPG), raised triglyceride (TG), and reduced HDL cholesterol) and each component of MetS were analyzed using multivariable logistic regression. Among the 1,115 obese Chinese adults with a mean age of 53.2(±7.2) years, serum irisin levels (log-transformed) were significantly reduced in subjects with MetS and raised FPG than their control groups (p = 0.034 and 0.041, respectively). After adjustment for potential confounders, serum irisin was significantly associated with reduced risks of MetS and raised FPG, with odds ratios (ORs) (95% CI) per standard deviation of log-transformed irisin of 0.796 (0.505-0.959, p = 0.027) and 0.873 (0.764-0.998, p = 0.046), respectively. Associations of irisin with raised BP, raised TG and reduced HDL were not statistically significant ((ORs) (95% CI): 0.733(0.454-1.182, p = 0.202), 0.954(0.838-1.086, p = 0.478) and 1.130(0.980-1.302, p = 0.092), respectively). Stepwise multivariable linear regression analysis showed that fasting insulin, HbA1c and albumin/globulin ratio were negatively associated with serum irisin level with statistical significance (all p-values
- Published
- 2014
- Full Text
- View/download PDF
5. Impaired lung function is associated with increased carotid intima-media thickness in middle-aged and elderly Chinese.
- Author
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Zhimin Ma, Yu Liu, Yu Xu, Yun Huang, Min Xu, Xiaolin Zhu, Huijie Zhang, Baihui Xu, Fei Huang, Zhi Yang, Xiaoying Li, Weiqing Wang, and Yufang Bi
- Subjects
Medicine ,Science - Abstract
BACKGROUND: Impairment of lung function was reported to be associated with cardiovascular disease (CVD). The aim of the present study is to evaluate the relationship between lung function and carotid intima-media thickness (cIMT) in participants without chronic pulmonary disease. METHODOLOGY AND PRINCIPAL FINDINGS: A total of 6,423 participants aged 40 years and above were recruited from Jiading District, Shanghai, China. Lung function, evaluated by forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) was measured with standard spirometry. CIMT was measured with high-resolution ultrasonography by trained physicians. Mean values of FVC (% pred) and FEV1 (% pred) in participants with elevated cIMT were significantly lower than in those without (0.92±0.20 vs. 0.99±0.19, 0.83±0.24 vs. 0.90±0.22; both p-values < 0.0001). The levels of cIMT in the lowest quartile of FVC (% pred) and FEV1 (% pred) were markedly higher than in the second, third and fourth quartile, respectively (p < 0.0001 for all). The lowest quartile of FVC (% pred) and FEV1 (% pred) was associated with increased odds of elevated cIMT, with the fully adjusted odds ratio of 1.34 and 1.41 (95% confidence interval (CI) 1.09-1.65, p = 0.006, 95% CI 1.15-1.72, p = 0.0008), respectively. CONCLUSIONS AND SIGNIFICANCE: Impaired lung function is associated with elevated cIMT in middle-aged and elderly Chinese. These findings suggest the need to screen impairment of lung function in people without respiratory disease for the presence of subclinical atherosclerosis in CVD prevention.
- Published
- 2013
- Full Text
- View/download PDF
6. Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells
- Author
-
Jie Wei, Peiying Deng, Meiyier Huandike, Jinyu Li, Juan Liu, Hening Chen, Xiaoying Meng, Changzhi Wang, Limin Chai, and Huijie Zhang
- Subjects
0301 basic medicine ,Cell signaling ,Physiology ,Anti-Inflammatory Agents ,Apoptosis ,Pharmacology ,Signal transduction ,Infographics ,T-Lymphocytes, Regulatory ,Fas ligand ,Antioxidants ,White Blood Cells ,Mice ,0302 clinical medicine ,Interferon ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,heterocyclic compounds ,Cells, Cultured ,Data Management ,Innate Immune System ,Mice, Inbred BALB C ,Multidisciplinary ,biology ,medicine.diagnostic_test ,Cell Death ,Chemistry ,T Cells ,Alkaloid ,Cell Differentiation ,Regulatory T cells ,T-Lymphocytes, Helper-Inducer ,Charts ,STAT signaling ,medicine.anatomical_structure ,Cell Processes ,030220 oncology & carcinogenesis ,Physical Sciences ,Cytokines ,Medicine ,Cellular Types ,medicine.drug ,Research Article ,Computer and Information Sciences ,Immune Cells ,Science ,Immunology ,Bone Marrow Cells ,Flow cytometry ,03 medical and health sciences ,Alkaloids ,medicine ,Animals ,Cell Proliferation ,Blood Cells ,Plant Extracts ,Data Visualization ,Chemical Compounds ,Biology and Life Sciences ,Cell Biology ,Coptis chinensis ,Molecular Development ,biology.organism_classification ,In vitro ,030104 developmental biology ,Immune System ,Bone marrow ,Developmental Biology ,Coptis ,Drugs, Chinese Herbal - Abstract
Coptidis alkaloids are the primary active components of Coptis chinensis Franch. Clinical and pharmacodynamic studies have confirmed that Coptidis alkaloids have multiple therapeutic effects including anti-inflammatory, antioxidant and antitumor effects, and they are usually used to treat various inflammatory disorders and related diseases. Mouse bone marrow cells (BMCs) were isolated from BALB/c mice. Immune-mediated destruction of BMCs was induced by interferon (IFN) -γ. High-performance liquid chromatography-electrospray ionization/ mass spectrometry was used to analyze the ingredients of the aqueous extract from Coptis chinensis Franch. The results confirmed that Coptidis alkaloids were the predominant ingredients in the aqueous extract from Coptis chinensis. The functional mechanism of Coptidis alkaloids in inhibiting immune-mediated destruction of BMCs was studied in vitro. After Coptidis alkaloid treatment, the percentages of apoptotic BMCs and the proliferation and differentiation of helper T (Th) cells and regulatory T (Treg) cells were measured by flow cytometry. The expression and distribution of T-bet in BMCs were observed by immunofluorescence. Western blotting analysis was used to assay the expression of key molecules in the Fas apoptosis and Jak/Stats signaling pathways in BMCs. We identified five alkaloids in the aqueous extract of Coptis chinensis. The apoptotic ratios of BMCs induced by IFN-γ were decreased significantly after Coptidis alkaloid treatment. The levels of key molecules (Fas, Caspase-3, cleaved Caspase-3, Caspase-8 and Caspase-8) in Fas apoptosis signaling pathways also decreased significantly after treatment with low concentrations of Coptidis alkaloids. Coptidis alkaloids were also found to inhibit the proliferation of Th1 and Th17 cells and induce the differentiation of Th2 and Treg cells; further, the distribution of T-bet in BMCs was decreased significantly. In addition, the levels of Stat-1, phospho-Stat-1 and phospho-Stat-3 were also reduced after Coptidis alkaloid treatment. These results indicate that Coptidis alkaloids extracted by water decoction from Coptis chinensis Franch could inhibit the proliferation and differentiation of T lymphocytes, attenuate the apoptosis of BMCs, and suppress the immune-mediated destruction of the BMCs induced by pro-inflammatory cytokines.
- Published
- 2020
7. Enhancing organic and inorganic carbon sequestration in calcareous soil by the combination of wheat straw and wood ash and/or lime
- Author
-
Xiaohong Tian, Songjie Chu, Yanlong Chen, Jifei Liu, Abdul Ghaffar Shar, Huili Zhao, and Huijie Zhang
- Subjects
Atmospheric Science ,010504 meteorology & atmospheric sciences ,lcsh:Medicine ,Plant Science ,Soil pH ,01 natural sciences ,Soil ,Plant Products ,Agricultural Soil Science ,lcsh:Science ,Triticum ,Lime ,Multidisciplinary ,Plant Stems ,Plant Anatomy ,Straw ,Eukaryota ,Soil chemistry ,Agriculture ,04 agricultural and veterinary sciences ,Hydrogen-Ion Concentration ,Plants ,Wood ,Chemistry ,Physical Sciences ,Wheat ,Engineering and Technology ,Calcareous ,Research Article ,Crops, Agricultural ,Carbon Sequestration ,Environmental Engineering ,Soil Science ,Crops ,engineering.material ,Zea mays ,Calcium Carbonate ,Greenhouse Gases ,Environmental Chemistry ,Grasses ,0105 earth and related environmental sciences ,Analysis of Variance ,Ecology and Environmental Sciences ,lcsh:R ,Chemical Compounds ,Organisms ,Biology and Life Sciences ,Wood ash ,Soil carbon ,Carbon Dioxide ,Carbon ,Agronomy ,Atmospheric Chemistry ,Earth Sciences ,040103 agronomy & agriculture ,engineering ,0401 agriculture, forestry, and fisheries ,Environmental science ,lcsh:Q ,Soil fertility ,Crop Science - Abstract
Increasing organic carbon sequestration in agricultural soils is important for improving soil fertility and mitigating climate change. Wood ash is generally applied as a potassium fertilizer, but the effects of simultaneous incorporation of wood ash and crop straw on the turnover of soil organic carbon (SOC) and inorganic carbon (SIC) are not well understood. In this study, a 118-day lab incubation experiment was conducted using a calcareous soil (with 10 years of continuous maize cropping history) to study the effects of adding wheat straw, wood ash and lime. Our study showed that straw addition led to an increase in both SOC (19%) and SIC (3%). Wood ash and lime addition decreased CO2 emission by 182 and 1210 mg kg-1 and increased SIC by 125 and 1001 mg kg-1 during the incubation, respectively, which was due to supply of CaO from wood ash and lime. The increase of SOC content was 2.4% due to the addition of lime. In addition to straw addition enhanced straw-derived OC content, the addition of lime also increased straw-derived OC content by 34.5%. This study demonstrated that lime was more effective in reducing CO2 emission and and enhancing SOC than wood ash. In conclusion, adding lime to calcareous soil might be an effective method of enhancing carbon sequestration and slowing climate change.
- Published
- 2018
8. Impaired lung function is associated with increased carotid intima-media thickness in middle-aged and elderly Chinese
- Author
-
Huijie Zhang, Yufang Bi, Baihui Xu, Xiaolin Zhu, Weiqing Wang, Zhi Yang, Xiaoying Li, Fei Huang, Min Xu, Zhimin Ma, Yun Huang, Yu Xu, and Yu Liu
- Subjects
Male ,Vital capacity ,Health Screening ,Epidemiology ,Vital Capacity ,lcsh:Medicine ,Cardiovascular ,Carotid Intima-Media Thickness ,Diagnostic Radiology ,Forced Expiratory Volume ,Clinical Epidemiology ,lcsh:Science ,Lung ,Ultrasonography ,Multidisciplinary ,medicine.diagnostic_test ,Respiratory disease ,Middle Aged ,respiratory system ,Carotid Arteries ,Quartile ,Cardiovascular Diseases ,Cardiology ,Observational Studies ,Medicine ,Female ,Public Health ,Radiology ,Research Article ,Spirometry ,medicine.medical_specialty ,Clinical Research Design ,FEV1/FVC ratio ,Internal medicine ,medicine ,Humans ,cardiovascular diseases ,Cardiovascular Disease Epidemiology ,Aged ,business.industry ,lcsh:R ,Odds ratio ,medicine.disease ,Atherosclerosis ,Confidence interval ,Surgery ,respiratory tract diseases ,Cross-Sectional Studies ,Intima-media thickness ,lcsh:Q ,business - Abstract
Background Impairment of lung function was reported to be associated with cardiovascular disease (CVD). The aim of the present study is to evaluate the relationship between lung function and carotid intima-media thickness (cIMT) in participants without chronic pulmonary disease. Methodology and Principal Findings A total of 6,423 participants aged 40 years and above were recruited from Jiading District, Shanghai, China. Lung function, evaluated by forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) was measured with standard spirometry. CIMT was measured with high-resolution ultrasonography by trained physicians. Mean values of FVC (% pred) and FEV1 (% pred) in participants with elevated cIMT were significantly lower than in those without (0.92±0.20 vs. 0.99±0.19, 0.83±0.24 vs. 0.90±0.22; both p-values < 0.0001). The levels of cIMT in the lowest quartile of FVC (% pred) and FEV1 (% pred) were markedly higher than in the second, third and fourth quartile, respectively (p < 0.0001 for all). The lowest quartile of FVC (% pred) and FEV1 (% pred) was associated with increased odds of elevated cIMT, with the fully adjusted odds ratio of 1.34 and 1.41 (95% confidence interval (CI) 1.09–1.65, p = 0.006, 95% CI 1.15–1.72, p = 0.0008), respectively. Conclusions and Significance Impaired lung function is associated with elevated cIMT in middle-aged and elderly Chinese. These findings suggest the need to screen impairment of lung function in people without respiratory disease for the presence of subclinical atherosclerosis in CVD prevention.
- Published
- 2013
9. Association of Serum Irisin with Metabolic Syndrome in Obese Chinese Adults
- Author
-
Yongwen Liu, Suhuan Liu, Shuyu Yang, Xiulin Shi, Ling-Ling Pan, Huijie Zhang, Bing Yan, Xiaoying Li, Zhibin Li, and Zhimin Ma
- Subjects
Adult ,Male ,medicine.medical_specialty ,Epidemiology ,Clinical Research Design ,medicine.medical_treatment ,Systems biology ,lcsh:Medicine ,Type 2 diabetes ,Research and Analysis Methods ,Endocrinology ,Insulin resistance ,Autosomal Recessive Diseases ,Asian People ,Internal medicine ,Genetics ,Medicine and Health Sciences ,medicine ,Humans ,Clinical Epidemiology ,Obesity ,lcsh:Science ,Adiposity ,Diabetic Endocrinology ,Metabolic Syndrome ,Molecular Epidemiology ,Multidisciplinary ,Population Biology ,business.industry ,Insulin ,lcsh:R ,Biology and Life Sciences ,Chinese adults ,Type 2 Diabetes Mellitus ,Middle Aged ,medicine.disease ,Fibronectins ,Cross-Sectional Studies ,Research Design ,Metabolic Disorders ,Female ,lcsh:Q ,Glycogen Storage Diseases ,Metabolic syndrome ,business ,Genetic Dominance ,Research Article - Abstract
Irisin, a recently identified novel myokine, drives brown-fat-like conversion of white adipose tissues and has been proposed to mediate beneficial effects of exercise on metabolism. Circulating irisin was significantly reduced in type 2 diabetes patients; however, no evidence is available about its association with metabolic syndrome (MetS) and effects of adiposity and muscle mass on circulating irisin have been controversial. Cross-sectional data on socio-demographic, lifestyle, clinical characteristics and serum irisin were collected for 1,115 community-living Chinese adults with central obesity. Associations of serum irisin with MetS (central obesity plus any two of the following four factors (raised blood pressure (BP), raised fasting plasma glucose (FPG), raised triglyceride (TG), and reduced HDL cholesterol) and each component of MetS were analyzed using multivariable logistic regression. Among the 1,115 obese Chinese adults with a mean age of 53.2(±7.2) years, serum irisin levels (log-transformed) were significantly reduced in subjects with MetS and raised FPG than their control groups (p = 0.034 and 0.041, respectively). After adjustment for potential confounders, serum irisin was significantly associated with reduced risks of MetS and raised FPG, with odds ratios (ORs) (95% CI) per standard deviation of log-transformed irisin of 0.796 (0.505–0.959, p = 0.027) and 0.873 (0.764–0.998, p = 0.046), respectively. Associations of irisin with raised BP, raised TG and reduced HDL were not statistically significant ((ORs) (95% CI): 0.733(0.454–1.182, p = 0.202), 0.954(0.838–1.086, p = 0.478) and 1.130(0.980–1.302, p = 0.092), respectively). Stepwise multivariable linear regression analysis showed that fasting insulin, HbA1c and albumin/globulin ratio were negatively associated with serum irisin level with statistical significance (all p-values
- Published
- 2014
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