21 results on '"Jia Zhu"'
Search Results
2. Role of post-chemotherapy radiation in the management of children and adolescents with primary advanced malignant mediastinal germ cell tumors.
- Author
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Junting Huang, Yuting Tan, Zijun Zhen, Suying Lu, Feifei Sun, Jia Zhu, Juan Wang, Ru Liao, and Xiaofei Sun
- Subjects
Medicine ,Science - Abstract
Primary malignant mediastinal germ cell tumors (MMGCTs) are rare in children and adolescents and have a poorer prognosis than their gonadal counterparts. We report a single institutional experience of a 10-year period of primary advanced MMGCTs treated with chemotherapy, followed by radiotherapy in those who had residual mass.Children and adolescents with primary advanced MMGCTs between 2005 and 2014 were identified from the Cancer Center, Sun Yat-Sen University. Medical records were reviewed for clinicopathological characteristics, treatments, and outcomes.Twenty-four children and adolescents with either stage III or IV primary advanced MMGCTs met the inclusion criteria. There were 23 males and one female with a median age of 16 (range 10-18). Seven cases were seminomas (29.2%); four (16.7%) yolk sac tumors (YST); three (12.5%) choriocarcinomas; and ten (41.6%) nonteratomatous combined germ cell tumors (CGCTs). All patients were treated with first-line cisplatin-based chemotherapy regimens (PEB: 19, VIP: 5). Thirteen (54.2%) and Twelve (50%) patients received surgery and radiotherapy, respectively. With a median follow-up of 46.2 months (range 9.6-124.8 months), a total of five (20%) patients died of disease progression; the five-year overall survival (OS) and disease-free survival (DFS) rates were 82.3% and 64.9%, respectively.-Seven patients with seminoma GCTs received post-chemotherapy irradiation were alive with sustained CR (5-year OS and DFS, 100%, respectively). Five patients with NSGCTs were administered irradiation and one relapsed 35 months later and died of metastasis (5-year OS, 100%; 5-year DFS 66.7%). Univariate analysis identified histology and stage were prognostic factors.Multimodality treatment approach of chemotherapy followed by radiation consolidation ensured long-term survival in primary advanced MMGCTs. Further research is warranted to improve the prognosis of children with primary advanced MMGCTs.
- Published
- 2017
- Full Text
- View/download PDF
3. In Situ Staining and Laser Capture Microdissection of Lymph Node Residing SIV Gag-Specific CD8+ T cells--A Tool to Interrogate a Functional Immune Response Ex Vivo.
- Author
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Annelie Tjernlund, Adam Burgener, Jessica M Lindvall, Tao Peng, Jia Zhu, Lars Öhrmalm, Louis J Picker, Kristina Broliden, M Juliana McElrath, and Lawrence Corey
- Subjects
Medicine ,Science - Abstract
While a plethora of data describes the essential role of systemic CD8+ T cells in the control of SIV replication little is known about the local in situ CD8+ T cell immune responses against SIV at the intact tissue level, due to technical limitations. In situ staining, using GagCM9 Qdot 655 multimers, were here combined with laser capture microdissection to detect and collect SIV Gag CM9 specific CD8+ T cells in lymph node tissue from SIV infected rhesus macaques. CD8+ T cells from SIV infected and uninfected rhesus macaques were also collected and compared to the SIV GagCM9 specific CD8+ T cells. Illumina bead array and transcriptional analyses were used to assess the transcriptional profiles and the three different CD8+ T cell populations displayed unique transcriptional patterns. This pilot study demonstrates that rapid and specific immunostaining combined with laser capture microdissection in concert with transcriptional profiling may be used to elucidate phenotypic differences between CD8+ T cells in SIV infection. Such technologies may be useful to determine differences in functional activities of HIV/SIV specific T cells.
- Published
- 2016
- Full Text
- View/download PDF
4. PFTK1 Promotes Gastric Cancer Progression by Regulating Proliferation, Migration and Invasion.
- Author
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Lei Yang, Jia Zhu, Hua Huang, Qichang Yang, Jing Cai, Qiuhong Wang, Junya Zhu, Mengting Shao, Jinzhang Xiao, Jie Cao, Xiaodan Gu, Shusen Zhang, and Yingying Wang
- Subjects
Medicine ,Science - Abstract
PFTK1, also known as PFTAIRE1, CDK14, is a novel member of Cdc2-related serine/threonine protein kinases. Recent studies show that PFTK1 is highly expressed in several malignant tumors such as hepatocellular carcinoma, esophageal cancer, breast cancer, and involved in regulation of cell cycle, tumors proliferation, migration, and invasion that further influence the prognosis of tumors. However, the expression and physiological significance of PFTK1 in gastric cancer remain unclear. In this study, we analyzed the expression and clinical significance of PFTK1 by Western blot in 8 paired fresh gastric cancer tissues, nontumorous gastric mucosal tissues and immunohistochemistry on 161 paraffinembedded slices. High PFTK1 expression was correlated with the tumor grade, lymph node invasion as well as Ki-67. Through Cell Counting Kit (CCK)-8 assay, flow cytometry, colony formation, wound healing and transwell assays, the vitro studies demonstrated that PFTK1 overexpression promoted proliferation, migration and invasion of gastric cancer cells, while PFTK1 knockdown led to the opposite results. Our findings for the first time supported that PFTK1 might play an important role in the regulation of gastric cancer proliferation, migration and would provide a novel promising therapeutic strategy against human gastric cancer.
- Published
- 2015
- Full Text
- View/download PDF
5. Insights into the cellular function of YhdE, a nucleotide pyrophosphatase from Escherichia coli.
- Author
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Jin Jin, Ruijuan Wu, Jia Zhu, Shaoyuan Yang, Zhen Lei, Nan Wang, Vinay K Singh, Jimin Zheng, and Zongchao Jia
- Subjects
Medicine ,Science - Abstract
YhdE, a Maf-like protein in Escherichia coli, exhibits nucleotide pyrophosphatase (PPase) activity, yet its cellular function remains unknown. Here, we characterized the PPase activity of YhdE on dTTP, UTP and TTP and determined two crystal structures of YhdE, revealing 'closed' and 'open' conformations of an adaptive active site. Our functional studies demonstrated that YhdE retards cell growth by prolonging the lag and log phases, particularly under stress conditions. Morphology studies showed that yhdE-knockout cells transformed the normal rod shape of wild-type cells to a more spherical form, and the cell wall appeared to become more flexible. In contrast, YhdE overexpression resulted in filamentous cells. This study reveals the previously unknown involvement of YhdE in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance, highlighting YhdE's important role in E. coli cell-cycle checkpoints.
- Published
- 2015
- Full Text
- View/download PDF
6. Altered fronto-striatal and fronto-cerebellar circuits in heroin-dependent individuals: a resting-state FMRI study.
- Author
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Yarong Wang, Jia Zhu, Qiang Li, Wei Li, Ning Wu, Ying Zheng, Haifeng Chang, Jiajie Chen, and Wei Wang
- Subjects
Medicine ,Science - Abstract
BACKGROUND: The formation of compulsive pattern of drug use is related to abnormal regional neural activity and functional reorganization in the heroin addicts' brain, but the relationship between heroin-use-induced disrupted local neural activity and its functional organization pattern in resting-state is unknown. METHODOLOGY/PRINCIPAL FINDINGS: With fMRI data acquired during resting state from 17 male heroin dependent individuals (HD) and 15 matched normal controls (NC), we analyzed the changes of amplitude of low frequency fluctuation (ALFF) in brain areas, and its relationship with history of heroin use. Then we investigated the addiction related alteration in functional connectivity of the brain regions with changed ALFF using seed-based correlation analysis. Compared with NC, the ALFF of HD was obviously decreased in the right caudate, right dorsal anterior cingulate cortex (dACC), right superior medial frontal cortex and increased in the bilateral cerebellum, left superior temporal gyrus and left superior occipital gyrus. Of the six regions, only the ALFF value of right caudate had a negative correlation with heroin use. Setting the six regions as "seeds", we found the functional connectivity between the right caudate and dorsolateral prefrontal cortex (dlPFC) was reduced but that between the right caudate and cerebellum was enhanced. Besides, an abnormal lateral PFC-dACC connection was also observed in HD. CONCLUSIONS: The observations of dysfunction of fronto-striatal and fronto-cerebellar circuit in HD implicate an altered balance between local neuronal assemblies activity and their integrated network organization pattern which may be involved in the process from voluntary to habitual and compulsive drug use.
- Published
- 2013
- Full Text
- View/download PDF
7. Assessing cue-induced brain response as a function of abstinence duration in heroin-dependent individuals: an event-related fMRI study.
- Author
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Qiang Li, Yarong Wang, Yi Zhang, Wei Li, Jia Zhu, Ying Zheng, Jiajie Chen, Liyan Zhao, Zhenyu Zhou, Yijun Liu, Wei Wang, and Jie Tian
- Subjects
Medicine ,Science - Abstract
The brain activity induced by heroin-related cues may play a role in the maintenance of heroin dependence. Whether the reinforcement or processing biases construct an everlasting feature of heroin addiction remains to be resolved. We used an event-related fMRI paradigm to measure brain activation in response to heroin cue-related pictures versus neutral pictures as the control condition in heroin-dependent patients undergoing short-term and long-term abstinence. The self-reported craving scores were significantly increased after cue exposure in the short-term abstinent patients (t = 3.000, P = 0.008), but no increase was found in the long-term abstinent patients (t = 1.510, P = 0.149). However, no significant differences in cue-induced craving changes were found between the two groups (t = 1.193, P = 0.850). Comparing between the long-term abstinence and short-term abstinence groups, significant decreases in brain activation were detected in the bilateral anterior cingulated cortex, left medial prefrontal cortex, caudate, middle occipital gyrus, inferior parietal lobule and right precuneus. Among all of the heroin dependent patients, the abstinence duration was negatively correlated with brain activation in the left medial prefrontal cortex and left inferior parietal lobule. These findings suggest that long-term abstinence may be useful for heroin-dependent patients to diminish their saliency value of heroin-related cues and possibly lower the relapse vulnerability to some extent.
- Published
- 2013
- Full Text
- View/download PDF
8. Identification, design and bio-evaluation of novel Hsp90 inhibitors by ligand-based virtual screening.
- Author
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JianMin Jia, XiaoLi Xu, Fang Liu, XiaoKe Guo, MingYe Zhang, MengChen Lu, LiLi Xu, JinLian Wei, Jia Zhu, ShengLie Zhang, ShengMiao Zhang, HaoPeng Sun, and QiDong You
- Subjects
Medicine ,Science - Abstract
Heat shock protein 90 (Hsp90), whose inhibitors have shown promising activity in clinical trials, is an attractive anticancer target. In this work, we first explored the significant pharmacophore features needed for Hsp90 inhibitors by generating a 3D-QSAR pharmacophore model. It was then used to virtually screen the SPECS databases, identifying 17 hits. Compound S1 and S13 exhibited the most potent inhibitory activity against Hsp90, with IC50 value 1.61±0.28 μM and 2.83±0.67 μM, respectively. Binding patterns analysis of the two compounds with Hsp90 revealed reasonable interaction modes. Further evaluation showed that the compounds exhibited good anti-proliferative effects against a series of cancer cell lines with high expression level of Hsp90. Meanwhile, S13 induced cell apoptosis in a dose-dependent manner in different cell lines. Based on the consideration of binding affinities, physicochemical properties and toxicities, 24 derivatives of S13 were designed, leading to the more promising compound S40, which deserves further optimization.
- Published
- 2013
- Full Text
- View/download PDF
9. Chronic zinc exposure decreases the surface expression of NR2A-containing NMDA receptors in cultured hippocampal neurons.
- Author
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Jia Zhu, Chong-Yu Shao, Wei Yang, Xiao-Min Zhang, Zhen-Yong Wu, Liang Zhou, Xin-Xin Wang, Yun-Hong Li, Jun Xia, Jian-Hong Luo, and Ying Shen
- Subjects
Medicine ,Science - Abstract
Zinc distributes widely in the central nervous system, especially in the hippocampus, amygdala and cortex. The dynamic balance of zinc is critical for neuronal functions. Zinc modulates the activity of N-methyl-D-aspartate receptors (NMDARs) through the direct inhibition and various intracellular signaling pathways. Abnormal NMDAR activities have been implicated in the aetiology of many brain diseases. Sustained zinc accumulation in the extracellular fluid is known to link to pathological conditions. However, the mechanism linking this chronic zinc exposure and NMDAR dysfunction is poorly understood.We reported that chronic zinc exposure reduced the numbers of NR1 and NR2A clusters in cultured hippocampal pyramidal neurons. Whole-cell and synaptic NR2A-mediated currents also decreased. By contrast, zinc did not affect NR2B, suggesting that chronic zinc exposure specifically influences NR2A-containg NMDARs. Surface biotinylation indicated that zinc exposure attenuated the membrane expression of NR1 and NR2A, which might arise from to the dissociation of the NR2A-PSD-95-Src complex.Chronic zinc exposure perturbs the interaction of NR2A to PSD-95 and causes the disorder of NMDARs in hippocampal neurons, suggesting a novel action of zinc distinct from its acute effects on NMDAR activity.
- Published
- 2012
- Full Text
- View/download PDF
10. Role of post-chemotherapy radiation in the management of children and adolescents with primary advanced malignant mediastinal germ cell tumors
- Author
-
Xiaofei Sun, Yuting Tan, Zijun Zhen, Junting Huang, Ru Liao, Suying Lu, Feifei Sun, Juan Wang, and Jia Zhu
- Subjects
Oncology ,Male ,medicine.medical_treatment ,Cancer Treatment ,lcsh:Medicine ,Metastasis ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Neoplasm Metastasis ,lcsh:Science ,Child ,Univariate analysis ,Multidisciplinary ,Pharmaceutics ,Neoplasms, Germ Cell and Embryonal ,Tumor Resection ,Combined Modality Therapy ,Surgical Oncology ,030220 oncology & carcinogenesis ,Female ,Cellular Types ,medicine.drug ,Research Article ,Clinical Oncology ,medicine.medical_specialty ,Adolescent ,Radiation Therapy ,Antineoplastic Agents ,Surgical and Invasive Medical Procedures ,Mediastinal Neoplasms ,Disease-Free Survival ,03 medical and health sciences ,Cancer Chemotherapy ,Drug Therapy ,Diagnostic Medicine ,Internal medicine ,medicine ,Cancer Detection and Diagnosis ,Humans ,Chemotherapy ,Cisplatin ,Surgical Resection ,business.industry ,lcsh:R ,Biology and Life Sciences ,Seminoma ,Cell Biology ,medicine.disease ,Mediastinal Neoplasm ,Surgery ,Radiation therapy ,Germ Cells ,lcsh:Q ,Germ cell tumors ,Clinical Medicine ,business ,030215 immunology - Abstract
Objective Primary malignant mediastinal germ cell tumors (MMGCTs) are rare in children and adolescents and have a poorer prognosis than their gonadal counterparts. We report a single institutional experience of a 10-year period of primary advanced MMGCTs treated with chemotherapy, followed by radiotherapy in those who had residual mass. Methods Children and adolescents with primary advanced MMGCTs between 2005 and 2014 were identified from the Cancer Center, Sun Yat-Sen University. Medical records were reviewed for clinicopathological characteristics, treatments, and outcomes. Results Twenty-four children and adolescents with either stage III or IV primary advanced MMGCTs met the inclusion criteria. There were 23 males and one female with a median age of 16 (range 10–18). Seven cases were seminomas (29.2%); four (16.7%) yolk sac tumors (YST); three (12.5%) choriocarcinomas; and ten (41.6%) nonteratomatous combined germ cell tumors (CGCTs). All patients were treated with first-line cisplatin-based chemotherapy regimens (PEB: 19, VIP: 5). Thirteen (54.2%) and Twelve (50%) patients received surgery and radiotherapy, respectively. With a median follow-up of 46.2 months (range 9.6–124.8 months), a total of five (20%) patients died of disease progression; the five-year overall survival (OS) and disease-free survival (DFS) rates were 82.3% and 64.9%, respectively.—Seven patients with seminoma GCTs received post-chemotherapy irradiation were alive with sustained CR (5-year OS and DFS, 100%, respectively). Five patients with NSGCTs were administered irradiation and one relapsed 35 months later and died of metastasis (5-year OS, 100%; 5-year DFS 66.7%). Univariate analysis identified histology and stage were prognostic factors. Conclusion Multimodality treatment approach of chemotherapy followed by radiation consolidation ensured long-term survival in primary advanced MMGCTs. Further research is warranted to improve the prognosis of children with primary advanced MMGCTs.
- Published
- 2016
11. Insights into the cellular function of YhdE, a nucleotide pyrophosphatase from Escherichia coli
- Author
-
Ruijuan Wu, Jimin Zheng, Jin Jin, Zhen Lei, Jia Zhu, Vinay K. Singh, Shaoyuan Yang, Nan Wang, and Zongchao Jia
- Subjects
Cell cycle checkpoint ,lcsh:Medicine ,Bacillus subtilis ,Biology ,medicine.disease_cause ,Cell wall ,chemistry.chemical_compound ,Escherichia coli ,medicine ,Pyrophosphatases ,lcsh:Science ,Multidisciplinary ,Cell growth ,Escherichia coli Proteins ,lcsh:R ,Active site ,Cell Cycle Checkpoints ,biology.organism_classification ,Cell biology ,chemistry ,Biochemistry ,Gene Knockdown Techniques ,biology.protein ,lcsh:Q ,Growth inhibition ,Function (biology) ,Research Article - Abstract
YhdE, a Maf-like protein in Escherichia coli, exhibits nucleotide pyrophosphatase (PPase) activity, yet its cellular function remains unknown. Here, we characterized the PPase activity of YhdE on dTTP, UTP and TTP and determined two crystal structures of YhdE, revealing ‘closed’ and ‘open’ conformations of an adaptive active site. Our functional studies demonstrated that YhdE retards cell growth by prolonging the lag and log phases, particularly under stress conditions. Morphology studies showed that yhdE-knockout cells transformed the normal rod shape of wild-type cells to a more spherical form, and the cell wall appeared to become more flexible. In contrast, YhdE overexpression resulted in filamentous cells. This study reveals the previously unknown involvement of YhdE in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance, highlighting YhdE’s important role in E. coli cell-cycle checkpoints.
- Published
- 2015
12. Hepatocyte-Specific Arid1a Deficiency Initiates Mouse Steatohepatitis and Hepatocellular Carcinoma
- Author
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Fang, Jia-Zhu, primary, Li, Chong, additional, Liu, Xiao-Yan, additional, Hu, Tao-Tao, additional, Fan, Zu-Sen, additional, and Han, Ze-Guang, additional
- Published
- 2015
- Full Text
- View/download PDF
13. Assessing Cue-Induced Brain Response as a Function of Abstinence Duration in Heroin-Dependent Individuals: An Event-Related fMRI Study
- Author
-
Yarong Wang, Liyan Zhao, Ying Zheng, Yi Zhang, Jia Zhu, Yijun Liu, Qiang Li, Zhenyu Zhou, Jie Tian, Wei Wang, Jiajie Chen, and Wei Li
- Subjects
Adult ,Male ,medicine.medical_specialty ,Time Factors ,Brain activity and meditation ,media_common.quotation_subject ,lcsh:Medicine ,Craving ,Biology ,Audiology ,Amygdala ,Cohort Studies ,Young Adult ,Cortex (anatomy) ,mental disorders ,medicine ,Humans ,Prefrontal cortex ,lcsh:Science ,media_common ,Behavior ,Multidisciplinary ,medicine.diagnostic_test ,Heroin Dependence ,lcsh:R ,Brain ,Inferior parietal lobule ,Abstinence ,Middle Aged ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Medicine ,lcsh:Q ,Female ,medicine.symptom ,Cues ,Functional magnetic resonance imaging ,Radiology ,Research Article - Abstract
The brain activity induced by heroin-related cues may play a role in the maintenance of heroin dependence. Whether the reinforcement or processing biases construct an everlasting feature of heroin addiction remains to be resolved. We used an event-related fMRI paradigm to measure brain activation in response to heroin cue-related pictures versus neutral pictures as the control condition in heroin-dependent patients undergoing short-term and long-term abstinence. The self-reported craving scores were significantly increased after cue exposure in the short-term abstinent patients (t = 3.000, P = 0.008), but no increase was found in the long-term abstinent patients (t = 1.510, P = 0.149). However, no significant differences in cue-induced craving changes were found between the two groups (t = 1.193, P = 0.850). Comparing between the long-term abstinence and short-term abstinence groups, significant decreases in brain activation were detected in the bilateral anterior cingulated cortex, left medial prefrontal cortex, caudate, middle occipital gyrus, inferior parietal lobule and right precuneus. Among all of the heroin dependent patients, the abstinence duration was negatively correlated with brain activation in the left medial prefrontal cortex and left inferior parietal lobule. These findings suggest that long-term abstinence may be useful for heroin-dependent patients to diminish their saliency value of heroin-related cues and possibly lower the relapse vulnerability to some extent.
- Published
- 2013
14. Hepatocyte-Specific Arid1a Deficiency Initiates Mouse Steatohepatitis and Hepatocellular Carcinoma
- Author
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Tao-Tao Hu, Chong Li, Ze-Guang Han, Zusen Fan, Xiao Yan Liu, and Jia-Zhu Fang
- Subjects
Lipopolysaccharides ,Genetically modified mouse ,Carcinoma, Hepatocellular ,ARID1A ,Tumor suppressor gene ,Carcinogenesis ,lcsh:Medicine ,Biology ,medicine.disease_cause ,Hepatitis ,medicine ,Animals ,Diethylnitrosamine ,lcsh:Science ,Mice, Knockout ,Multidisciplinary ,lcsh:R ,Liver Neoplasms ,Fatty liver ,Nuclear Proteins ,Interleukin ,medicine.disease ,Immunity, Innate ,DNA-Binding Proteins ,Fatty Liver ,Liver ,Organ Specificity ,Hepatocytes ,Cancer research ,Cytokines ,lcsh:Q ,Tumor necrosis factor alpha ,Inflammation Mediators ,Steatohepatitis ,Transcription Factors ,Research Article - Abstract
ARID1A, encoding a subunit of chromatin remodeling SWI/SNF complexes, has recently been considered as a new type of tumor suppressor gene for its somatic mutations frequently found in various human tumors, including hepatocellular carcinoma (HCC). However, the role and mechanism of inactivated ARID1A mutations in tumorigenesis remain unclear. To investigate the role of ARID1A inactivation in HCC pathogenesis, we generated hepatocyte-specific Arid1a knockout (Arid1a LKO) mice by crossing mice carrying loxP-flanked Arid1a exon 8 alleles (Arid1a f/f) with albumin promoter-Cre transgenic mice. Significantly, the hepatocyte-specific Arid1a deficiency results in mouse steatohepatitis and HCC development. In Arid1a LKO mice, we found that innate immune cells, including F4/80+ macrophages and CD11c+ neutrophil cells, infiltrate into the liver parenchyma, accompanied by the increased tumor necrosis factor (TNF)-α and interleukin (IL)-6, and activation of STAT3 and NF-κB pathways. In conclusion, hepatocyte-specific Arid1a deficiency could lead to mouse steatohepatitis and HCC development. This study provides an alternative mechanism by which Arid1a deficiency contributes to HCC tumorigenesis.
- Published
- 2015
15. PFTK1 Promotes Gastric Cancer Progression by Regulating Proliferation, Migration and Invasion
- Author
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Junya Zhu, Jia Zhu, Jie Cao, Shusen Zhang, Yingying Wang, Hua Huang, Mengting Shao, Xiaodan Gu, Qichang Yang, Qiuhong Wang, Lei Yang, Jinzhang Xiao, and Jing Cai
- Subjects
Male ,Pathology ,medicine.medical_specialty ,lcsh:Medicine ,Biology ,Breast cancer ,Cell Movement ,Stomach Neoplasms ,Cyclin-dependent kinase ,Cell Line, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,RNA, Small Interfering ,lcsh:Science ,Lymph node ,Cell Proliferation ,Multidisciplinary ,Kinase ,lcsh:R ,Stomach ,Cancer ,Middle Aged ,Cell cycle ,Esophageal cancer ,Prognosis ,medicine.disease ,Cyclin-Dependent Kinases ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,Cancer cell ,Disease Progression ,biology.protein ,lcsh:Q ,Female ,RNA Interference ,Research Article - Abstract
PFTK1, also known as PFTAIRE1, CDK14, is a novel member of Cdc2-related serine/threonine protein kinases. Recent studies show that PFTK1 is highly expressed in several malignant tumors such as hepatocellular carcinoma, esophageal cancer, breast cancer, and involved in regulation of cell cycle, tumors proliferation, migration, and invasion that further influence the prognosis of tumors. However, the expression and physiological significance of PFTK1 in gastric cancer remain unclear. In this study, we analyzed the expression and clinical significance of PFTK1 by Western blot in 8 paired fresh gastric cancer tissues, nontumorous gastric mucosal tissues and immunohistochemistry on 161 paraffinembedded slices. High PFTK1 expression was correlated with the tumor grade, lymph node invasion as well as Ki-67. Through Cell Counting Kit (CCK)-8 assay, flow cytometry, colony formation, wound healing and transwell assays, the vitro studies demonstrated that PFTK1 overexpression promoted proliferation, migration and invasion of gastric cancer cells, while PFTK1 knockdown led to the opposite results. Our findings for the first time supported that PFTK1 might play an important role in the regulation of gastric cancer proliferation, migration and would provide a novel promising therapeutic strategy against human gastric cancer.
- Published
- 2015
16. Identification, Design and Bio-Evaluation of Novel Hsp90 Inhibitors by Ligand-Based Virtual Screening
- Author
-
Xiao-Li Xu, Jia Zhu, Jinlian Wei, Shengmiao Zhang, Meng-Chen Lu, Jian-Min Jia, Fang Liu, Li-Li Xu, Guo Xiaoke, Shenglie Zhang, Qidong You, Ming-Ye Zhang, and Haopeng Sun
- Subjects
Models, Molecular ,Databases, Factual ,Drug Evaluation, Preclinical ,Molecular Conformation ,lcsh:Medicine ,Quantitative Structure-Activity Relationship ,Plasma protein binding ,Pharmacology ,Ligands ,Biochemistry ,Computer Applications ,Computational Chemistry ,Molecular Cell Biology ,lcsh:Science ,Multidisciplinary ,biology ,Chemistry ,Hsp90 ,Computer-Aided Design ,Pharmacophore ,Protein Binding ,Research Article ,Signal Transduction ,Computer Modeling ,Quantitative structure–activity relationship ,Antineoplastic Agents ,Inhibitory Concentration 50 ,Cell Line, Tumor ,Heat shock protein ,Chemical Biology ,Humans ,Computer Simulation ,HSP90 Heat-Shock Proteins ,Binding site ,Theoretical Chemistry ,Biology ,Virtual screening ,Binding Sites ,Ligand ,lcsh:R ,Organic Chemistry ,Proteins ,Computational Biology ,Acute Phase Proteins ,Combinatorial chemistry ,Drug Design ,Computer Science ,biology.protein ,lcsh:Q ,Medicinal Chemistry - Abstract
Heat shock protein 90 (Hsp90), whose inhibitors have shown promising activity in clinical trials, is an attractive anticancer target. In this work, we first explored the significant pharmacophore features needed for Hsp90 inhibitors by generating a 3D-QSAR pharmacophore model. It was then used to virtually screen the SPECS databases, identifying 17 hits. Compound S1 and S13 exhibited the most potent inhibitory activity against Hsp90, with IC50 value 1.61±0.28 μM and 2.83±0.67 μM, respectively. Binding patterns analysis of the two compounds with Hsp90 revealed reasonable interaction modes. Further evaluation showed that the compounds exhibited good anti-proliferative effects against a series of cancer cell lines with high expression level of Hsp90. Meanwhile, S13 induced cell apoptosis in a dose-dependent manner in different cell lines. Based on the consideration of binding affinities, physicochemical properties and toxicities, 24 derivatives of S13 were designed, leading to the more promising compound S40, which deserves further optimization.
- Published
- 2013
17. Altered Fronto-Striatal and Fronto-Cerebellar Circuits in Heroin-Dependent Individuals: A Resting-State fMRI Study
- Author
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Jiajie Chen, Jia Zhu, Ning Wu, Wei Wang, Qiang Li, Wei Li, Ying Zheng, Yarong Wang, and Haifeng Chang
- Subjects
Male ,Cerebellum ,Anatomy and Physiology ,Nerve net ,lcsh:Medicine ,Social and Behavioral Sciences ,Diagnostic Radiology ,Psychology ,lcsh:Science ,Psychiatry ,Multidisciplinary ,medicine.diagnostic_test ,Heroin Dependence ,Substance Abuse ,Magnetic Resonance Imaging ,Frontal Lobe ,Mental Health ,medicine.anatomical_structure ,Neurology ,Frontal lobe ,Medicine ,medicine.symptom ,Radiology ,Research Article ,Adult ,medicine.medical_specialty ,Behavioral addiction ,Rest ,Neuroimaging ,Neurological System ,Neuropsychology ,mental disorders ,medicine ,Humans ,Biology ,Anterior cingulate cortex ,Demography ,Resting state fMRI ,business.industry ,lcsh:R ,Corpus Striatum ,Dorsolateral prefrontal cortex ,Neuroanatomy ,nervous system ,Case-Control Studies ,lcsh:Q ,Nerve Net ,Functional magnetic resonance imaging ,business ,Neuroscience - Abstract
Background The formation of compulsive pattern of drug use is related to abnormal regional neural activity and functional reorganization in the heroin addicts’ brain, but the relationship between heroin-use-induced disrupted local neural activity and its functional organization pattern in resting-state is unknown. Methodology/Principal Findings With fMRI data acquired during resting state from 17 male heroin dependent individuals (HD) and 15 matched normal controls (NC), we analyzed the changes of amplitude of low frequency fluctuation (ALFF) in brain areas, and its relationship with history of heroin use. Then we investigated the addiction related alteration in functional connectivity of the brain regions with changed ALFF using seed-based correlation analysis. Compared with NC, the ALFF of HD was obviously decreased in the right caudate, right dorsal anterior cingulate cortex (dACC), right superior medial frontal cortex and increased in the bilateral cerebellum, left superior temporal gyrus and left superior occipital gyrus. Of the six regions, only the ALFF value of right caudate had a negative correlation with heroin use. Setting the six regions as “seeds”, we found the functional connectivity between the right caudate and dorsolateral prefrontal cortex (dlPFC) was reduced but that between the right caudate and cerebellum was enhanced. Besides, an abnormal lateral PFC-dACC connection was also observed in HD. Conclusions The observations of dysfunction of fronto-striatal and fronto-cerebellar circuit in HD implicate an altered balance between local neuronal assemblies activity and their integrated network organization pattern which may be involved in the process from voluntary to habitual and compulsive drug use.
- Published
- 2013
18. Chronic Zinc Exposure Decreases the Surface Expression of NR2A-Containing NMDA Receptors in Cultured Hippocampal Neurons
- Author
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Zhen-Yong Wu, Liang Zhou, Wei Yang, Jun Xia, Xin-Xin Wang, Ying Shen, Chong-Yu Shao, Jianhong Luo, Xiao-min Zhang, Yunhong Li, and Jia Zhu
- Subjects
Anatomy and Physiology ,lcsh:Medicine ,Gene Expression ,Tetrazolium Salts ,Hippocampus ,Hippocampal formation ,Membrane Potentials ,Rats, Sprague-Dawley ,Molecular Cell Biology ,lcsh:Science ,Receptor ,Neurons ,Cerebral Cortex ,Multidisciplinary ,Cell Death ,musculoskeletal, neural, and ocular physiology ,Intracellular Signaling Peptides and Proteins ,Brain ,Amygdala ,Mitochondria ,Cell biology ,Electrophysiology ,Zinc ,medicine.anatomical_structure ,Biochemistry ,Cerebral cortex ,NMDA receptor ,Cellular Types ,Disks Large Homolog 4 Protein ,psychological phenomena and processes ,Research Article ,Cell Survival ,Surface Properties ,Central nervous system ,chemistry.chemical_element ,Biology ,Signaling Pathways ,Models, Biological ,Receptors, N-Methyl-D-Aspartate ,Neurological System ,Quinoxalines ,mental disorders ,medicine ,Animals ,Biotinylation ,Dose-Response Relationship, Drug ,lcsh:R ,Membrane Proteins ,Rats ,Cortex (botany) ,Thiazoles ,nervous system ,chemistry ,Cellular Neuroscience ,Synapses ,lcsh:Q ,Nimodipine ,Molecular Neuroscience ,Neuroscience - Abstract
Background Zinc distributes widely in the central nervous system, especially in the hippocampus, amygdala and cortex. The dynamic balance of zinc is critical for neuronal functions. Zinc modulates the activity of N-methyl-D-aspartate receptors (NMDARs) through the direct inhibition and various intracellular signaling pathways. Abnormal NMDAR activities have been implicated in the aetiology of many brain diseases. Sustained zinc accumulation in the extracellular fluid is known to link to pathological conditions. However, the mechanism linking this chronic zinc exposure and NMDAR dysfunction is poorly understood. Methodology/Principal Findings We reported that chronic zinc exposure reduced the numbers of NR1 and NR2A clusters in cultured hippocampal pyramidal neurons. Whole-cell and synaptic NR2A-mediated currents also decreased. By contrast, zinc did not affect NR2B, suggesting that chronic zinc exposure specifically influences NR2A-containg NMDARs. Surface biotinylation indicated that zinc exposure attenuated the membrane expression of NR1 and NR2A, which might arise from to the dissociation of the NR2A-PSD-95-Src complex. Conclusions Chronic zinc exposure perturbs the interaction of NR2A to PSD-95 and causes the disorder of NMDARs in hippocampal neurons, suggesting a novel action of zinc distinct from its acute effects on NMDAR activity.
- Published
- 2012
19. αA Crystallin May Protect against Geographic Atrophy--Meta-Analysis of Cataract vs. Cataract Surgery for Geographic Atrophy and Experimental Studies.
- Author
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Peng Zhou, Hong-Fei Ye, Yong-Xiang Jiang, Jin Yang, Xiang-Jia Zhu, Xing-Huai Sun, Yi Luo, Guo-Rui Dou, Yu-Sheng Wang, Yi Lu, and Vavvas, Demetrios
- Subjects
RETINAL diseases ,RETINAL degeneration ,META-analysis ,CATARACT surgery ,IMMUNOHISTOCHEMISTRY ,OXIDATIVE stress ,DEGENERATION (Pathology) - Abstract
Background: Cataract and geographic atrophy (GA, also called advanced "dry" age-related macular degeneration) are the two major causes of visual impairment in the developed world. The association between cataract surgery and the development of GA was controversial in previous studies. Methods/Principal Findings: We performed a meta-analysis by pooling the current evidence in literature and found that cataract is associated with an increased risk of geographic atrophy with a summary odds ratio (OR) of 3.75 (95% CI: 95% CI: 1.84- 7.62). However, cataract surgery is not associated with the risk of geographic atrophy (polled OR = 3.23, 95% CI: 0.6316.47). Further experiments were performed to analyze how the αA-crystallin, the major component of the lens, influences the development of GA in a mouse model. We found that the αA-crystallin mRNA and protein expression increased after oxidative stress induced by NalO
3 in immunohistochemistry of retinal section and western blot of posterior eyecups. Both functional and histopathological evidence confirmed that GA is more severe in αA-crystallin knockout mice compared to wild-type mice. Conclusions: Therefore, αA-crystallin may protect against geographic atrophy. This study provides a better understanding of the relationship between cataract, cataract surgery, and GA. [ABSTRACT FROM AUTHOR]- Published
- 2012
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20. Hepatocyte-Specific Arid1a Deficiency Initiates Mouse Steatohepatitis and Hepatocellular Carcinoma.
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Jia-Zhu Fang, Chong Li, Xiao-Yan Liu, Tao-Tao Hu, Zu-Sen Fan, and Ze-Guang Han
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Medicine ,Science - Abstract
ARID1A, encoding a subunit of chromatin remodeling SWI/SNF complexes, has recently been considered as a new type of tumor suppressor gene for its somatic mutations frequently found in various human tumors, including hepatocellular carcinoma (HCC). However, the role and mechanism of inactivated ARID1A mutations in tumorigenesis remain unclear. To investigate the role of ARID1A inactivation in HCC pathogenesis, we generated hepatocyte-specific Arid1a knockout (Arid1aLKO) mice by crossing mice carrying loxP-flanked Arid1a exon 8 alleles (Arid1af/f) with albumin promoter-Cre transgenic mice. Significantly, the hepatocyte-specific Arid1a deficiency results in mouse steatohepatitis and HCC development. In Arid1aLKO mice, we found that innate immune cells, including F4/80+ macrophages and CD11c+ neutrophil cells, infiltrate into the liver parenchyma, accompanied by the increased tumor necrosis factor (TNF)-α and interleukin (IL)-6, and activation of STAT3 and NF-κB pathways. In conclusion, hepatocyte-specific Arid1a deficiency could lead to mouse steatohepatitis and HCC development. This study provides an alternative mechanism by which Arid1a deficiency contributes to HCC tumorigenesis.
- Published
- 2015
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21. Epigenetic regulation of αA-crystallin in high myopia-induced dark nuclear cataract.
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Xiang-Jia Zhu, Peng Zhou, Ke-Ke Zhang, Jin Yang, Yi Luo, and Yi Lu
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Medicine ,Science - Abstract
PURPOSE: To assess the etiology of early-onset dark nucleus in high-myopic patients and its relationship with the epigenetic regulation of αA-crystallin (CRYAA). METHODS: We reviewed clinical data from patients who underwent cataract surgery at our center in 2012. Lens epithelial samples were collected during capsulorhexis, whereas young lens epithelium was donated. Cataract type and severity were graded according to the Lens Opacity Classification System III (LOCS III). DNA methylation was analyzed by pyrosequencing the CpG islands of the CRYAA promoter in the following groups: Age-Related Cataract (ARC) Nuclear Color (NC) 2-3; High-Myopic Cataract (HMC) NC2-3; ARC NC5-6; HMC NC5-6; and in young lenses graded NC1. We analyzed CRYAA expression by real-time polymerase chain reaction (PCR), reverse transcription PCR, and immunohistochemistry. RESULTS: The odds ratio of dark nucleus in high-myopic patients was 5.16 (95% confidence interval: 3.98-6.69; p
- Published
- 2013
- Full Text
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