8 results on '"Jiasen Cheng"'
Search Results
2. A secretory protein of necrotrophic fungus Sclerotinia sclerotiorum that suppresses host resistance.
- Author
-
Wenjun Zhu, Wei Wei, Yanping Fu, Jiasen Cheng, Jiatao Xie, Guoqing Li, Xianhong Yi, Zhensheng Kang, Martin B Dickman, and Daohong Jiang
- Subjects
Medicine ,Science - Abstract
SSITL (SS1G_14133) of Sclerotinia sclerotiorum encodes a protein with 302 amino acid residues including a signal peptide, its secretion property was confirmed with immunolocalization and immunofluorescence techniques. SSITL was classified in the integrin alpha N-terminal domain superfamily, and its 3D structure is similar to those of human integrin α4-subunit and a fungal integrin-like protein. When S. sclerotiorum was inoculated to its host, high expression of SSITL was detected during the initial stages of infection (1.5-3.0 hpi). Targeted silencing of SSITL resulted in a significant reduction in virulence; on the other hand, inoculation of SSITL silenced transformant A10 initiated strong and rapid defense response in Arabidopsis, the highest expressions of defense genes PDF1.2 and PR-1 appeared at 3 hpi which was 9 hr earlier than that time when plants were inoculated with the wild-type strain of S. sclerotiorum. Systemic resistance induced by A10 was detected by analysis of the expression of PDF1.2 and PR-1, and confirmed following inoculation with Botrytis cinerea. A10 induced much larger lesions on Arabidopsis mutant ein2 and jar1, and slightly larger lesions on mutant pad4 and NahG in comparison with the wild-type plants. Furthermore, both transient and constitutive expression of SSITL in Arabidopsis suppressed the expression of PDF1.2 and led to be more susceptible to A10 and the wild-type strain of S. sclerotiorum and B. cinerea. Our results suggested that SSITL is an effector possibly and plays significant role in the suppression of jasmonic/ethylene (JA/ET) signal pathway mediated resistance at the early stage of infection.
- Published
- 2013
- Full Text
- View/download PDF
3. Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum.
- Author
-
Yang Yu, Daohong Jiang, Jiatao Xie, Jiasen Cheng, Guoqing Li, Xianhong Yi, and Yanping Fu
- Subjects
Medicine ,Science - Abstract
The sclerotium is an important dormant body for many plant fungal pathogens. Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant homology with any protein of known function. Ss-Sl2 contains two putative PAN modules which were found in other proteins with diverse adhesion functions. Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced strains were not significantly different from the wild strain. Ss-Sl2 silenced strains could form interwoven hyphal masses at the initial stage of sclerotial development, but the interwoven hyphae could not consolidate and melanize. Hyphae in these interwoven bodies were thin-walled, and arranged loosely. Co-immunoprecipitation and yeast two-hybrid experiments showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Woronin body major protein (Hex1) and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced strains. Hex1-knockdown strains showed similar impairment in maintenance of hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 functions in both sclerotial development and cellular integrity of S. sclerotiorum.
- Published
- 2012
- Full Text
- View/download PDF
4. Discovery of novel dsRNA viral sequences by in silico cloning and implications for viral diversity, host range and evolution.
- Author
-
Huiquan Liu, Yanping Fu, Jiatao Xie, Jiasen Cheng, Said A Ghabrial, Guoqing Li, Xianhong Yi, and Daohong Jiang
- Subjects
Medicine ,Science - Abstract
Genome sequence of viruses can contribute greatly to the study of viral evolution, diversity and the interaction between viruses and hosts. Traditional molecular cloning methods for obtaining RNA viral genomes are time-consuming and often difficult because many viruses occur in extremely low titers. DsRNA viruses in the families, Partitiviridae, Totiviridae, Endornaviridae, Chrysoviridae, and other related unclassified dsRNA viruses are generally associated with symptomless or persistent infections of their hosts. These characteristics indicate that samples or materials derived from eukaryotic organisms used to construct cDNA libraries and EST sequencing might carry these viruses, which were not easily detected by the researchers. Therefore, the EST databases may include numerous unknown viral sequences. In this study, we performed in silico cloning, a procedure for obtaining full or partial cDNA sequence of a gene by bioinformatics analysis, using known dsRNA viral sequences as queries to search against NCBI Expressed Sequence Tag (EST) database. From this analysis, we obtained 119 novel virus-like sequences related to members of the families, Endornaviridae, Chrysoviridae, Partitiviridae, and Totiviridae. Many of them were identified in cDNA libraries of eukaryotic lineages, which were not known to be hosts for these viruses. Furthermore, comprehensive phylogenetic analysis of these newly discovered virus-like sequences with known dsRNA viruses revealed that these dsRNA viruses may have co-evolved with respective host supergroups over a long evolutionary time while potential horizontal transmissions of viruses between different host supergroups also is possible. We also found that some of the plant partitiviruses may have originated from fungal viruses by horizontal transmissions. These findings extend our knowledge of the diversity and possible host range of dsRNA viruses and offer insight into the origin and evolution of relevant viruses with their hosts.
- Published
- 2012
- Full Text
- View/download PDF
5. Endosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae
- Author
-
Zhixiao Gao, Daohong Jiang, Huiquan Liu, Jiatao Xie, Jiasen Cheng, Yanping Fu, Kai Bi, Tao Chen, Ying Zhao, and Binnian Tian
- Subjects
0301 basic medicine ,Biological pest control ,Brassica ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Plant Science ,Biochemistry ,Polymerase Chain Reaction ,Plant Roots ,Database and Informatics Methods ,Cluster Analysis ,lcsh:Science ,education.field_of_study ,Multidisciplinary ,biology ,Microbiota ,food and beverages ,Eukaryota ,Genomics ,Nucleic acids ,Ribosomal RNA ,Medical Microbiology ,medicine.symptom ,Sequence Analysis ,Research Article ,Cell biology ,Cellular structures and organelles ,Bioinformatics ,Population ,Sequence Databases ,Microbial Genomics ,Research and Analysis Methods ,Real-Time Polymerase Chain Reaction ,Asymptomatic ,Microbiology ,Clubroot ,03 medical and health sciences ,medicine ,Genetics ,Microbiome ,Internal transcribed spacer ,education ,Non-coding RNA ,Molecular Biology Techniques ,Cercozoa ,Molecular Biology ,Biology and life sciences ,Bacteria ,lcsh:R ,Brassica napus ,Organisms ,Fungi ,Plant Pathology ,medicine.disease ,biology.organism_classification ,16S ribosomal RNA ,030104 developmental biology ,Biological Databases ,RNA ,lcsh:Q ,Ribosomes - Abstract
Clubroot caused by Plasmodiophora brassicae, is a severe disease of cruciferous crops that causes large hypertrophic galls in the roots. The plant microbiome is important for growth promotion and disease suppression. In this study, using 16S rRNA and internal transcribed spacer (ITS) sequencing techniques, we compared the endosphere microbiome of symptomatic and asymptomatic B. napus roots infected with P. brassicae collected from the same natural clubroot field. The results showed that the microbial population and its relative abundance in the asymptomatic roots was far higher than that in the symptomatic roots, and that many microorganisms in asymptomatic roots have biological control and plant growth promotion functions that may be related to clubroot symptoms. These results suggest the importance of the endosphere microbiome in clubroot disease and provide potential bio-control resources for its prevention.
- Published
- 2017
6. A secretory protein of necrotrophic fungus Sclerotinia sclerotiorum that suppresses host resistance
- Author
-
Guoqing Li, Xianhong Yi, Zhensheng Kang, Wei Wei, Jiasen Cheng, Yanping Fu, Daohong Jiang, Jiatao Xie, Martin B. Dickman, and Wenjun Zhu
- Subjects
Signal peptide ,Models, Molecular ,Integrins ,Protein Conformation ,Fungal Physiology ,Science ,Molecular Sequence Data ,Plant Pathogens ,Arabidopsis ,Mycology ,Pathogenesis ,Plant Science ,Microbiology ,Fungal Proteins ,Plant Microbiology ,Ascomycota ,Cell Wall ,Gene Expression Regulation, Fungal ,Arabidopsis thaliana ,Humans ,Amino Acid Sequence ,Gene Silencing ,Fungal Biochemistry ,Biology ,Botrytis cinerea ,Disease Resistance ,Plant Diseases ,Fungal protein ,Multidisciplinary ,biology ,Effector ,Sclerotinia sclerotiorum ,Fungal genetics ,Botany ,Fungal Diseases ,Plant Pathology ,biology.organism_classification ,Host-Pathogen Interaction ,Infectious Diseases ,Phenotype ,Host-Pathogen Interactions ,Medicine ,Extracellular Space ,Research Article ,HeLa Cells - Abstract
SSITL (SS1G_14133) of Sclerotinia sclerotiorum encodes a protein with 302 amino acid residues including a signal peptide, its secretion property was confirmed with immunolocalization and immunofluorescence techniques. SSITL was classified in the integrin alpha N-terminal domain superfamily, and its 3D structure is similar to those of human integrin α4-subunit and a fungal integrin-like protein. When S. sclerotiorum was inoculated to its host, high expression of SSITL was detected during the initial stages of infection (1.5–3.0 hpi). Targeted silencing of SSITL resulted in a significant reduction in virulence; on the other hand, inoculation of SSITL silenced transformant A10 initiated strong and rapid defense response in Arabidopsis, the highest expressions of defense genes PDF1.2 and PR-1 appeared at 3 hpi which was 9 hr earlier than that time when plants were inoculated with the wild-type strain of S. sclerotiorum. Systemic resistance induced by A10 was detected by analysis of the expression of PDF1.2 and PR-1, and confirmed following inoculation with Botrytis cinerea. A10 induced much larger lesions on Arabidopsis mutant ein2 and jar1, and slightly larger lesions on mutant pad4 and NahG in comparison with the wild-type plants. Furthermore, both transient and constitutive expression of SSITL in Arabidopsis suppressed the expression of PDF1.2 and led to be more susceptible to A10 and the wild-type strain of S. sclerotiorum and B. cinerea. Our results suggested that SSITL is an effector possibly and plays significant role in the suppression of jasmonic/ethylene (JA/ET) signal pathway mediated resistance at the early stage of infection.
- Published
- 2013
7. Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial development and cellular integrity of Sclerotinia sclerotiorum
- Author
-
Jiasen Cheng, Yanping Fu, Daohong Jiang, Xianhong Yi, Guoqing Li, Jiatao Xie, and Yang Yu
- Subjects
Hyphal growth ,Sclerotium ,Hypha ,Science ,Molecular Sequence Data ,Hyphae ,Plant Pathogens ,Mycology ,Plant Science ,Real-Time Polymerase Chain Reaction ,Microbiology ,Fungal Proteins ,Peptide Elongation Factor 1 ,Plant Microbiology ,Woronin body ,Ascomycota ,Cell Wall ,Tandem Mass Spectrometry ,Two-Hybrid System Techniques ,Genetics ,Immunoprecipitation ,Fungal Biochemistry ,Biology ,Microbial Pathogens ,Mycelium ,Melanins ,Fungal protein ,Multidisciplinary ,biology ,Base Sequence ,Virulence ,Reverse Transcriptase Polymerase Chain Reaction ,Sclerotinia sclerotiorum ,Glyceraldehyde-3-Phosphate Dehydrogenases ,Sequence Analysis, DNA ,Plant Pathology ,biology.organism_classification ,Medicine ,RNA Interference ,Gene Function ,Polyketide Synthases ,Sequence Alignment ,Chromatography, Liquid ,Research Article - Abstract
The sclerotium is an important dormant body for many plant fungal pathogens. Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant homology with any protein of known function. Ss-Sl2 contains two putative PAN modules which were found in other proteins with diverse adhesion functions. Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced strains were not significantly different from the wild strain. Ss-Sl2 silenced strains could form interwoven hyphal masses at the initial stage of sclerotial development, but the interwoven hyphae could not consolidate and melanize. Hyphae in these interwoven bodies were thin-walled, and arranged loosely. Co-immunoprecipitation and yeast two-hybrid experiments showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Woronin body major protein (Hex1) and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced strains. Hex1-knockdown strains showed similar impairment in maintenance of hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 functions in both sclerotial development and cellular integrity of S. sclerotiorum.
- Published
- 2012
8. Discovery of Novel dsRNA Viral Sequences by In Silico Cloning and Implications for Viral Diversity, Host Range and Evolution
- Author
-
Guoqing Li, Said A. Ghabrial, Daohong Jiang, Jiatao Xie, Huiquan Liu, Xianhong Yi, Jiasen Cheng, and Yanping Fu
- Subjects
Evolutionary Genetics ,RNA viruses ,Evolutionary Processes ,Sequence analysis ,viruses ,Fungal Viruses ,lcsh:Medicine ,Forms of Evolution ,Microbiology ,Divergent Evolution ,Viral Evolution ,Evolution, Molecular ,Genome Analysis Tools ,Viral classification ,Virology ,Animals ,Cloning, Molecular ,lcsh:Science ,Biology ,Phylogeny ,Gene Library ,RNA, Double-Stranded ,Genetics ,Evolutionary Biology ,Expressed sequence tag ,Multidisciplinary ,biology ,lcsh:R ,Computational Biology ,Genomics ,Biodiversity ,Comparative Genomics ,Partitiviridae ,biology.organism_classification ,Organismal Evolution ,Endornaviridae ,Viral replication ,Viral evolution ,Microbial Evolution ,Host-Pathogen Interactions ,Totiviridae ,lcsh:Q ,Sequence Analysis ,Research Article ,Coevolution - Abstract
Genome sequence of viruses can contribute greatly to the study of viral evolution, diversity and the interaction between viruses and hosts. Traditional molecular cloning methods for obtaining RNA viral genomes are time-consuming and often difficult because many viruses occur in extremely low titers. DsRNA viruses in the families, Partitiviridae, Totiviridae, Endornaviridae, Chrysoviridae, and other related unclassified dsRNA viruses are generally associated with symptomless or persistent infections of their hosts. These characteristics indicate that samples or materials derived from eukaryotic organisms used to construct cDNA libraries and EST sequencing might carry these viruses, which were not easily detected by the researchers. Therefore, the EST databases may include numerous unknown viral sequences. In this study, we performed in silico cloning, a procedure for obtaining full or partial cDNA sequence of a gene by bioinformatics analysis, using known dsRNA viral sequences as queries to search against NCBI Expressed Sequence Tag (EST) database. From this analysis, we obtained 119 novel virus-like sequences related to members of the families, Endornaviridae, Chrysoviridae, Partitiviridae, and Totiviridae. Many of them were identified in cDNA libraries of eukaryotic lineages, which were not known to be hosts for these viruses. Furthermore, comprehensive phylogenetic analysis of these newly discovered virus-like sequences with known dsRNA viruses revealed that these dsRNA viruses may have co-evolved with respective host supergroups over a long evolutionary time while potential horizontal transmissions of viruses between different host supergroups also is possible. We also found that some of the plant partitiviruses may have originated from fungal viruses by horizontal transmissions. These findings extend our knowledge of the diversity and possible host range of dsRNA viruses and offer insight into the origin and evolution of relevant viruses with their hosts.
- Published
- 2012
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