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10 results on '"Koschmieder, Steffen"'

4. Identification of the Adapter Molecule MTSS1 as a Potential Oncogene-Specific Tumor Suppressor in Acute Myeloid Leukemia

Author

Schemionek, Mirle, primary, Kharabi Masouleh, Behzad, additional, Klaile, Yvonne, additional, Krug, Utz, additional, Hebestreit, Katja, additional, Schubert, Claudia, additional, Dugas, Martin, additional, Büchner, Thomas, additional, Wörmann, Bernhard, additional, Hiddemann, Wolfgang, additional, Berdel, Wolfgang E., additional, Brümmendorf, Tim H., additional, Müller-Tidow, Carsten, additional, and Koschmieder, Steffen, additional

Published
2015
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5. Dissecting Genomic Aberrations in Myeloproliferative Neoplasms by Multiplex-PCR and Next Generation Sequencing

Author

Kirschner, Martin M. J., primary, Schemionek, Mirle, additional, Schubert, Claudia, additional, Chatain, Nicolas, additional, Sontag, Stephanie, additional, Isfort, Susanne, additional, Ortiz-Brüchle, Nadina, additional, Schmitt, Karla, additional, Krüger, Luisa, additional, Zerres, Klaus, additional, Zenke, Martin, additional, Brümmendorf, Tim H., additional, and Koschmieder, Steffen, additional

Published
2015
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6. Feasibility of Azacitidine Added to Standard Chemotherapy in Older Patients with Acute Myeloid Leukemia — A Randomised SAL Pilot Study

Author

Krug, Utz, primary, Koschmieder, Anja, additional, Schwammbach, Daniela, additional, Gerss, Joachim, additional, Tidow, Nicola, additional, Steffen, Björn, additional, Bug, Gesine, additional, Brandts, Christian H., additional, Schaich, Markus, additional, Röllig, Christoph, additional, Thiede, Christian, additional, Noppeney, Richard, additional, Stelljes, Matthias, additional, Büchner, Thomas, additional, Koschmieder, Steffen, additional, Dührsen, Ulrich, additional, Serve, Hubert, additional, Ehninger, Gerhard, additional, Berdel, Wolfgang E., additional, and Müller-Tidow, Carsten, additional

Published
2012
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7. Mutations of the EPHB6 Receptor Tyrosine Kinase Induce a Pro-Metastatic Phenotype in Non-Small Cell Lung Cancer

Author

Bulk, Etmar, primary, Yu, Jun, additional, Hascher, Antje, additional, Koschmieder, Steffen, additional, Wiewrodt, Rainer, additional, Krug, Utz, additional, Timmermann, Bernd, additional, Marra, Alessandro, additional, Hillejan, Ludger, additional, Wiebe, Karsten, additional, Berdel, Wolfgang E., additional, Schwab, Albrecht, additional, and Müller-Tidow, Carsten, additional

Published
2012
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10. Construction and Application of an Inducible System for Homogenous Expression Levels in Bulk Cell Lines.

Author

Jun Yu, Mller, Helena, Hehn, Sina, Koschmieder, Steffen, Kai Schönig, Berdel, Wolfgang E., Serve, Hubert, and Müller-Tidow, Carsten

Subjects
CELL culture, CELL lines, CELLS, GENE expression, GENETIC regulation, LUNG cancer, GREEN fluorescent protein, GENETIC engineering, HEREDITY
Abstract

Stringently controlled conditional expressing systems are crucial for the functional characterization of genes. Currently, screening of multiple clones to identify the tightly controlled ones is necessary but time-consuming. Here, we describe a system fusing Tet (tetracycline)-inducible elements, BAC (bacterial artificial chromosome) and Gateway technology together to allow tight control of gene expression in BAC-transfected eukaryotic bulk cell cultures. Recombinase cloning into the shuttle vector and the BAC facilitates vector construction. An EGFP (enhanced green fluorescent protein) allows FACS (fluorescence activated cell sorting) and the BAC technology ensures tight control of gene expression that is independent of the integrating site. In the current first application, our gene of interest encodes a β-catenin-ERα fusion protein. Tested by luciferase assay and western blotting, in HTB56 lung cancer cells the final BAC E11-IGR-β-catenin-ERα vector demonstrated sensitive inducibility by Tet or Dox (doxycycline) in a dose-dependent manner with low background, and the EGFP was an effective selection marker by FACS in bulk culture HTB56 and myeloblastic 32D cells. This is a highly efficient tool for the rapid generation of stringently controlled Tet-inducible systems in cell lines. [ABSTRACT FROM AUTHOR]

Published
2009
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