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Your search keyword '"Li, Jinhui"' showing total 7 results
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7 results on '"Li, Jinhui"'

1. Involvement of the JNK/FOXO3a/Bim Pathway in Neuronal Apoptosis after Hypoxic-Ischemic Brain Damage in Neonatal Rats.

Author

Li, Deyuan, Li, Xihong, Wu, Jinlin, Li, Jinhui, Zhang, Li, Xiong, Tao, Tang, Jun, Qu, Yi, and Mu, Dezhi

Subjects
Brain, Neurons, Cell Nucleus, Animals, Animals, Newborn, Rats, Rats, Sprague-Dawley, Hypoxia-Ischemia, Brain, Acetonitriles, JNK Mitogen-Activated Protein Kinases, Membrane Proteins, Proto-Oncogene Proteins, Phosphoproteins, Apoptosis, MAP Kinase Signaling System, Gene Expression Regulation, Enzymologic, Up-Regulation, Active Transport, Cell Nucleus, Phosphorylation, Female, Male, Apoptosis Regulatory Proteins, Forkhead Transcription Factors, Benzothiazoles, Bcl-2-Like Protein 11, Forkhead Box Protein O3, Active Transport, Newborn, Gene Expression Regulation, Enzymologic, Hypoxia-Ischemia, Sprague-Dawley, General Science & Technology
Abstract

c-Jun N-terminal kinase (JNK) plays a key role in the regulation of neuronal apoptosis. Previous studies have revealed that forkhead transcription factor (FOXO3a) is a critical effector of JNK-mediated tumor suppression. However, it is not clear whether the JNK/FOXO3a pathway is involved in neuronal apoptosis in the developing rat brain after hypoxia-ischemia (HI). In this study, we generated an HI model using postnatal day 7 rats. Fluorescence immunolabeling and Western blot assays were used to detect the distribution and expression of total and phosphorylated JNK and FOXO3a and the pro-apoptotic proteins Bim and CC3. We found that JNK phosphorylation was accompanied by FOXO3a dephosphorylation, which induced FOXO3a translocation into the nucleus, resulting in the upregulation of levels of Bim and CC3 proteins. Furthermore, we found that JNK inhibition by AS601245, a specific JNK inhibitor, significantly increased FOXO3a phosphorylation, which attenuated FOXO3a translocation into the nucleus after HI. Moreover, JNK inhibition downregulated levels of Bim and CC3 proteins, attenuated neuronal apoptosis and reduced brain infarct volume in the developing rat brain. Our findings suggest that the JNK/FOXO3a/Bim pathway is involved in neuronal apoptosis in the developing rat brain after HI. Agents targeting JNK may offer promise for rescuing neurons from HI-induced damage.

Published
2015

4. Rapid and sensitive detection of Senecavirus A by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick method

Author

Yu Li, Zhou Xia, Dongsheng He, Li Jinhui, Jian Shi, Bowen Liu, Shuaifei Xu, Jingfeng Xiong, Si Guangbin, and Liang Weifang

Subjects
0301 basic medicine, Swine, Artificial Gene Amplification and Extension, Picornaviridae, Polymerase Chain Reaction, Biochemistry, Polymerases, Gel Electrophoresis, Mammals, Multidisciplinary, Chemistry, Eukaryota, Molecular Diagnostic Techniques, Agarose gel electrophoresis, Vertebrates, Engineering and Technology, RNA, Viral, Medicine, RNA extraction, Nucleic Acid Amplification Techniques, Research Article, Biotechnology, Cognitive Neuroscience, Science, 030106 microbiology, Agarose Gel Electrophoresis, Loop-mediated isothermal amplification, Bioengineering, DNA polymerase, Research and Analysis Methods, Sensitivity and Specificity, 03 medical and health sciences, Electrophoretic Techniques, Extraction techniques, DNA-binding proteins, Reaction Time, Animals, Molecular Biology Techniques, Reverse Transcription Loop-mediated Isothermal Amplification, Molecular Biology, Detection limit, Organisms, Biology and Life Sciences, Proteins, Nucleic acid amplification technique, Dipstick, Reverse Transcriptase-Polymerase Chain Reaction, Reverse Transcription, Molecular biology, Reverse transcriptase, 030104 developmental biology, Amniotes, Cognitive Science, Neuroscience
Abstract

Senecavirus A (SVA) is a critical pathogen causing vesicular lesions in sows and acute death of newborn piglets, resulting in very large economic losses in the pig industry. To restrict the transmission of SVA, an establishment of an effective diagnostic method is crucial for the prevention and control of the disease. However, traditional detection methods often have many drawbacks. In this study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was combined with a lateral flow dipstick (LFD) to detect SVA. The resulting RT-LAMP-LFD assay was performed at 60°C for 50 min and then directly judged on an LFD visualization strip. This method shows high specificity and sensitivity to SVA. The detection limit of RT-LAMP was 4.56x10-8 ng/μL RNA, approximately 11 copies/μL RNA, and it was 10 times more sensitive than RT-PCR. This detection method’s positive rate for clinical samples is comparable to that of RT-PCR. This method is time saving and highly efficient and is thus expected to be used to diagnose SVA infections in this field.

Published
2019

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