254 results on '"Li, Ye"'
Search Results
2. Measuring intangible cultural heritage image: A scale development.
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Liu, Yuqing, Li, Ye, Tao, Wenjie, and Wang, Qingsheng
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CULTURAL property , *LITERATURE reviews , *QUANTITATIVE research , *SUSTAINABLE tourism , *QUALITATIVE research , *EXPLORATORY factor analysis - Abstract
Although an increasing number of studies have examined issues relating to the preservation and development of intangible cultural heritage (ICH), there has been limited research on how tourists perceive ICH. Moreover, UNESCO asserts that the concept of "authenticity" is not applicable to ICH, and so far, no valid instrument for measuring tourists' subjective perceptions of ICH has been developed, even though their perceptions play a very important role in the preservation and development of ICH. Therefore, this paper aims to develop a measurement scale for ICH image, using both qualitative and quantitative research methods. Participant observation, semi-structured in-depth interviews, secondary data collection, and a literature review were conducted to generate the initial scale items, and then the main surveys were conducted to collect data for the model tests. Four dimensions were extracted by exploratory factor analysis: transmission, localization, vitality, and association. The reliability and validity of the measurement model were demonstrated through confirmatory factor analysis. We further determined that the transmission, vitality, and association of ICH image have a positive impact on tourists' revisit intention. The paper highlights the crucial role of ICH image in sustainable tourism development. The theoretical and managerial implications of the study are discussed, followed by suggestions for future research. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Sex difference in driving speed management: The mediation effect of impulse control
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Pan, Chunyang, primary, Ma, Jinfei, additional, Li, Ye, additional, Lu, Yang, additional, Shan, Lixin, additional, and Chang, Ruosong, additional
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- 2023
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4. High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China.
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Yu-Jie Wei, Yun Wu, Yin-Zhuo Yan, Wan Zou, Jie Xue, Wen-Rui Ma, Wei Wang, Ge Tian, and Li-Ye Wang
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Medicine ,Science - Abstract
In this study Illumina MiSeq was performed to investigate microbial diversity in soil, leaves, grape, grape juice and wine. A total of 1,043,102 fungal Internal Transcribed Spacer (ITS) reads and 2,422,188 high quality bacterial 16S rDNA sequences were used for taxonomic classification, revealed five fungal and eight bacterial phyla. At the genus level, the dominant fungi were Ascomycota, Sordariales, Tetracladium and Geomyces in soil, Aureobasidium and Pleosporaceae in grapes leaves, Aureobasidium in grape and grape juice. The dominant bacteria were Kaistobacter, Arthrobacter, Skermanella and Sphingomonas in soil, Pseudomonas, Acinetobacter and Kaistobacter in grape and grapes leaves, and Oenococcus in grape juice and wine. Principal coordinate analysis showed structural separation between the composition of fungi and bacteria in all samples. This is the first study to understand microbiome population in soil, grape, grapes leaves, grape juice and wine in Xinjiang through High-throughput Sequencing and identify microorganisms like Saccharomyces cerevisiae and Oenococcus spp. that may contribute to the quality and flavor of wine.
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- 2018
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5. Differential induction of apoptosis and autophagy by pyrrolizidine alkaloid clivorine in human hepatoma Huh-7.5 cells and its toxic implication.
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Wenju Liu, Xu Li, Bo Zhou, Shoucai Fang, Wenzhe Ho, Hui Chen, Hao Liang, Li Ye, and Jun Tang
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Medicine ,Science - Abstract
Growing evidence suggests that the pyrrolizidine alkaloids (PAs)-induced hepatotoxicity is mediated by multiple cell death/defence modalities. However, the detailed mechanisms are still lacking. In this study, the hepatotoxic effects of four PAs including three retronecine-type ones (senecionine, seneciphylline and monocrotaline) and one otonecine-type (clivorine) on the proliferation of Huh-7.5 cells and the possible mechanisms were investigated. The results showed that all the PAs could inhibit cell proliferation and induce apoptosis in a concentration-dependent manner. Among them clivorine was the most significant one. In addition to its effect on apoptosis, clivorine treatment could promote autophagy in Huh-7.5 cells, as evidenced by the accumulation of autophagosomes, the enhancement of LC3B expression at the concentrations close to its IC0 value, and the increased conversion of LC3B-I to LC3B-II in the presence of lysosomal inhibitor (chloroquine) and decreased formation of green fluorescent protein (GFP)-LC3 positive puncta in the presence of autophagic sequestration inhibitor (3-methyladenine). Among the other tested PAs, senecionine and seneciphylline also activated autophagy at the same concentrations used for clivorine but monocrotaline did not. Furthermore, our study demonstrated that suppression or enhancement of autophagy resulted in the remarkable enhancement or suppression of senecionine, seneciphylline and clivorine-induced apoptosis at the concentration close to the IC10 for clivorine, respectively, indicating a protective role of autophagy against the PA-induced apoptosis at the low level of exposure. Collectively, our data suggest that PAs in different structures may exert different toxic disturbances on the liver cells. Apoptosis may be one of the most common models of the PA-induced cytotoxicity, while autophagy may be a structure-dependent defence model in the early stage of PA intoxication. Differential induction of apoptosis and autophagy probably depending on the concentration is essential for the cytotoxic potency of clivorine.
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- 2017
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6. Identifying the changing age distribution of opioid-related mortality with high-frequency data
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Paul, Lauren A., primary, Li, Ye, additional, Leece, Pamela, additional, Gomes, Tara, additional, Bayoumi, Ahmed M., additional, Herring, Jeremy, additional, Murray, Regan, additional, and Brown, Patrick, additional
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- 2022
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7. Benefits and Challenges with Applying Unique Molecular Identifiers in Next Generation Sequencing to Detect Low Frequency Mutations.
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Ruqin Kou, Ham Lam, Hairong Duan, Li Ye, Narisra Jongkam, Weizhi Chen, Shifang Zhang, and Shihong Li
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Medicine ,Science - Abstract
Indexing individual template molecules with a unique identifier (UID) before PCR and deep sequencing is promising for detecting low frequency mutations, as true mutations could be distinguished from PCR errors or sequencing errors based on consensus among reads sharing same index. In an effort to develop a robust assay to detect from urine low-abundant bladder cancer cells carrying well-documented mutations, we have tested the idea first on a set of mock templates, with wild type and known mutants mixed at defined ratios. We have measured the combined error rate for PCR and Illumina sequencing at each nucleotide position of three exons, and demonstrated the power of a UID in distinguishing and correcting errors. In addition, we have demonstrated that PCR sampling bias, rather than PCR errors, challenges the UID-deep sequencing method in faithfully detecting low frequency mutation.
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- 2016
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8. Effect of Pioglitazone in Preventing In-Stent Restenosis after Percutaneous Coronary Intervention in Patients with Type 2 Diabetes: A Meta-Analysis.
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Shi-Jie Zhao, Zhao-Shuang Zhong, Guo-Xian Qi, Li-Ye Shi, Ling Chen, and Wen Tian
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Medicine ,Science - Abstract
BACKGROUND:The benefits of pioglitazone in patients with type 2 diabetes mellitus (T2DM) after percutaneous coronary intervention (PCI) is unclear. OBJECTIVES:To evaluate the effect of pioglitazone on prevention of in-stent restenosis (ISR) in patients with T2DM after PCI. METHODS:All full-text published relevant studies compared the effect of pioglitazone with control group (placebo or no pioglitazone treatment) on ISR in patients with T2DM after PCI were identified by searching the databases including PubMed, EMBASE, Cochrane Library and ISI Web of Science through October 2015. The endpoints were defined as the rate of ISR, late lumen loss, in-stent neointimal volume, target lesion revascularization (TLR) and major adverse cardiac events (MACE). RESULTS:Six studies (5 RCTs and 1 retrospective study), comprising 503 patients, were included into this meta-analysis. In the pioglitazone group, as compared with the control group, the risk ratio for ISR was 0.48 (I2 = 14.5%, P = 0.322; 95%CI 0.35 to 0.68, P
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- 2016
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9. Application of a Combined Model with Autoregressive Integrated Moving Average (ARIMA) and Generalized Regression Neural Network (GRNN) in Forecasting Hepatitis Incidence in Heng County, China.
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Wudi Wei, Junjun Jiang, Hao Liang, Lian Gao, Bingyu Liang, Jiegang Huang, Ning Zang, Yanyan Liao, Jun Yu, Jingzhen Lai, Fengxiang Qin, Jinming Su, Li Ye, and Hui Chen
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Medicine ,Science - Abstract
BACKGROUND:Hepatitis is a serious public health problem with increasing cases and property damage in Heng County. It is necessary to develop a model to predict the hepatitis epidemic that could be useful for preventing this disease. METHODS:The autoregressive integrated moving average (ARIMA) model and the generalized regression neural network (GRNN) model were used to fit the incidence data from the Heng County CDC (Center for Disease Control and Prevention) from January 2005 to December 2012. Then, the ARIMA-GRNN hybrid model was developed. The incidence data from January 2013 to December 2013 were used to validate the models. Several parameters, including mean absolute error (MAE), root mean square error (RMSE), mean absolute percentage error (MAPE) and mean square error (MSE), were used to compare the performance among the three models. RESULTS:The morbidity of hepatitis from Jan 2005 to Dec 2012 has seasonal variation and slightly rising trend. The ARIMA(0,1,2)(1,1,1)12 model was the most appropriate one with the residual test showing a white noise sequence. The smoothing factor of the basic GRNN model and the combined model was 1.8 and 0.07, respectively. The four parameters of the hybrid model were lower than those of the two single models in the validation. The parameters values of the GRNN model were the lowest in the fitting of the three models. CONCLUSIONS:The hybrid ARIMA-GRNN model showed better hepatitis incidence forecasting in Heng County than the single ARIMA model and the basic GRNN model. It is a potential decision-supportive tool for controlling hepatitis in Heng County.
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- 2016
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10. The roles of endoplasmic reticulum overload response induced by HCV and NS4B protein in human hepatocyte viability and virus replication.
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Lingbao Kong, Shanshan Li, Mingjie Huang, Ying Xiong, Qinghua Zhang, Li Ye, Jing Liu, Xiangdong Zhu, Ruina Sun, and Yunli Guo
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Medicine ,Science - Abstract
Hepatitis C virus (HCV) replication is associated with endoplasmic reticulum (ER) and its infection triggers ER stress. In response to ER stress, ER overload response (EOR) can be activated, which involves the release of Ca2+ from ER, production of reactive oxygen species (ROS) and activation of nuclear factor κB (NF-κB). We have previously reported that HCV NS4B expression activates NF-κB via EOR-Ca2+-ROS pathway. Here, we showed that NS4B expression and HCV infection activated cancer-related NF-κB signaling pathway and induced the expression of cancer-related NF-κB target genes via EOR-Ca2+-ROS pathway. Moreover, we found that HCV-activated EOR-Ca2+-ROS pathway had profound effects on host cell viability and HCV replication. HCV infection induced human hepatocyte death by EOR-Ca2+-ROS pathway, whereas activation of EOR-Ca2+-ROS-NF-κB pathway increased the cell viability. Meanwhile, EOR-Ca2+-ROS-NF-κB pathway inhibited acute HCV replication, which could alleviate the detrimental effect of HCV on cell viability and enhance chronic HCV infection. Together, our findings provide new insights into the functions of EOR-Ca2+-ROS-NF-κB pathway in natural HCV replication and pathogenesis.
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- 2015
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11. Multiple Genetic Modifiers of Bilirubin Metabolism Involvement in Significant Neonatal Hyperbilirubinemia in Patients of Chinese Descent.
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Hui Yang, Qian Wang, Lei Zheng, Min Lin, Xiang-bin Zheng, Fen Lin, and Li-Ye Yang
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Medicine ,Science - Abstract
The potential for genetic variation to modulate neonatal hyperbilirubinemia risk is increasingly being recognized. A case-control study was designed to assess comprehensive contributions of the multiple genetic modifiers of bilirubin metabolism on significant neonatal hyperbilirubinemia in Chinese descendents. Eleven common mutations and polymorphisms across five bilirubin metabolism genes, namely those encoding UGT1A1, HMOX1, BLVRA, SLCO1B1 and SLCO1B3, were determined using the high resolution melt (HRM) assay or PCR-capillary electrophoresis analysis. A total of 129 hyperbilirubinemic infants and 108 control subjects were evaluated. Breastfeeding and the presence of the minor A allele of rs4148323 (UGTA*6) were correlated with an increased risk of hyperbilirubinemia (OR=2.17, P=0.02 for breastfeeding; OR=9.776, P=0.000 for UGTA*6 homozygote; OR=3.151, P=0.000 for UGTA*6 heterozygote); whereas, increasing gestational age and the presence of -TA7 repeat variant of UGT1A1 decreased the risk (OR=0.721, P=0.003 for gestational age; OR=0.313, P=0.002 for heterozygote TA6/TA7). In addition, the SLCO1B1 and SLCO1B3 polymorphisms also contributed to an increased risk of hyperbilirubinemia. This detailed analysis revealed the impact of multiple genetic modifiers on neonatal hyperbilirubinemia. This may support the use of genetic tests for clinical risk assessment. Furthermore, the established HRM assay can serve as an effective method for large-scale investigation.
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- 2015
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12. Seroprevalence of Human Immunodeficiency Virus, Hepatitis B Virus, Hepatitis C Virus, and Treponema pallidum Infections among Blood Donors on Bioko Island, Equatorial Guinea.
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Dong-De Xie, Jian Li, Jiang-Tao Chen, Urbano Monsuy Eyi, Rocio Apicante Matesa, Maximo Miko Ondo Obono, Carlos Sala Ehapo, Li-Ye Yang, Hui Yang, Hui-Tian Yang, and Min Lin
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Medicine ,Science - Abstract
Regular screening of transfusion-transmissible infections (TTIs), such as human immunodeficiency virus (HIV), hepatitis B and hepatitis C virus (HBV and HCV, respectively), and Treponema pallidum, in blood donors is essential to guaranteeing clinical transfusion safety. This study aimed to determine the seroprevalence of four TTIs among blood donors on Bioko Island, Equatorial Guinea (EG).A retrospective survey of blood donors from January 2011 to April 2013 was conducted to assess the presence of HIV, HBV, HCV and T. pallidum. The medical records were analyzed to verify the seroprevalence of these TTIs among blood donations stratified by gender, age and geographical region.Of the total 2937 consecutive blood donors, 1098 (37.39%) had a minimum of one TTI and 185 (6.29%) harbored co-infections. The general seroprevalence of HIV, HBV, HCV and T. pallidum were 7.83%, 10.01%, 3.71% and 21.51%, respectively. The most frequent TTI co-infections were HBV-T. pallidum 60 (2.04%) and HIV-T. pallidum 46 (1.57%). The seroprevalence of HIV, HBV, HCV and T. pallidum were highest among blood donors 38 to 47 years, 18 to 27 years and ≥ 48 years age, respectively (P
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- 2015
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13. Acceptability of Male Circumcision among College Students in Medical Universities in Western China: A Cross-Sectional Study.
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Junjun Jiang, Jinming Su, Xiaobo Yang, Mingbo Huang, Wei Deng, Jiegang Huang, Bingyu Liang, Bo Qin, Halmurat Upur, Chaohui Zhong, Qianqiu Wang, Qian Wang, Yuhua Ruan, Li Ye, and Hao Liang
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Medicine ,Science - Abstract
Male circumcision (MC) has been shown to reduce the risk of female to male transmission of HIV. The goal of this survey was to explore MC's acceptability and the factors associated with MC among college students in medical universities in western China.A cross-sectional study was carried out in three provinces in western China (Guangxi, Chongqing and Xinjiang) to assess the acceptability of MC as well as to discover factors associated with the acceptability among college students in medical universities. A total of 1,790 uncircumcised male students from three medical universities were enrolled in this study. In addition, 150 students who had undergone MC were also enrolled in the survey, and they participated in in-depth interviews.Of all the uncircumcised participants (n = 1,790), 55.2% (n = 988) were willing to accept MC. Among those who accepted MC, 67.3% thought that MC could improve their sexual partners' hygiene, 46.3% believed that HIV and sexually transmitted diseases (STDs) could be partially prevented by MC. The multivariable logistic regression indicates that MC's acceptability was associated with three factors: the redundant foreskin (OR = 10.171, 95% CI = 7.629-13.559), knowing the hazard of having a redundant foreskin (OR = 1.597, 95% CI = 1.097-2.323), and enhancing sexual pleasure (OR = 1.628, 95% CI = 1.312-2.021). The in-depth interviews for subjects who had undergone MC showed that the major reason for having MC was the redundant foreskin (87.3%), followed by the benefits and the fewer complications of having MC done. In addition, most of these participants (65.3%) said that the MC could enhance sexual satisfaction.MC's acceptance among college students in medical universities is higher than it is among other populations in western China. An implementation of an MC programme among this population is feasible in the future.
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- 2015
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14. G6PD Deficiency and Hemoglobinopathies: Molecular Epidemiological Characteristics and Healthy Effects on Malaria Endemic Bioko Island, Equatorial Guinea.
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Min Lin, Li Ye Yang, Dong De Xie, Jiang Tao Chen, Santiago-m Monte Nguba, Carlos Sala Ehapo, Xiao Fen Zhan, Juan Urbano Monsuy Eyi, Rocio Apicante Matesa, Maximo Miko Ondo Obono, Hui Yang, Hui Tian Yang, and Ji Dong Cheng
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Medicine ,Science - Abstract
Glucose-6-phosphate dehydrogenase (G6PD) deficiency and hemoglobinopathies were the inherited conditions found mostly in African. However, few epidemiological data of these disorders was reported in Equatorial Guinea (EQG). This study aimed to assess the prevalence and healthy effects of G6PD deficiency and hemoglobinopathies among the people on malaria endemic Bioko Island, EQG.Blood samples from 4,144 unrelated subjects were analyzed for G6PD deficiency by fluorescence spot test (FST), high-resolution melting assay and PCR-DNA sequencing. In addition, 1,186 samples were randomly selected from the 4,144 subjects for detection of hemoglobin S (HbS), HbC, and α-thalassemia deletion by complete blood count, PCR-DNA sequencing and reverse dot blot (RDB).The prevalence of malaria and anemia was 12.6% (522/4,144) and 32.8% (389/1,186), respectively. Overall, 8.7% subjects (359/4,144) were G6PD-deficient by FST, including 9.0% (249/2,758) males and 7.9% (110/1,386) females. Among the 359 G6PD-deficient individuals molecularly studied, the G6PD A- (G202A/A376G) were detected in 356 cases (99.2%), G6PD Betica (T968C/A376G) in 3 cases. Among the 1,186 subjects, 201 cases were HbS heterozygotes, 35 cases were HbC heterozygotes, and 2 cases were HbCS double heterozygotes; 452 cases showed heterozygous α-thalassemia 3.7 kb deletion (-α3.7 kb deletion) and 85 homozygous - α3.7 kb deletion. The overall allele frequencies were HbS 17.1% (203/1186); HbC, 3.1% (37/1186); and -α3.7 kb deletion 52.4% (622/1186), respectively.High G6PD deficiency in this population indicate that diagnosis and management of G6PD deficiency is necessary on Bioko Island. Obligatory newborn screening, prenatal screening and counseling for these genetic disorders, especially HbS, are needed on the island.
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- 2015
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15. Molecular epidemiological characterization and health burden of thalassemia in Jiangxi Province, P. R. China.
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Min Lin, Tian-Yu Zhong, Yi-Guo Chen, Jian-Zhong Wang, Jiao-Ren Wu, Fen Lin, Xin Tong, Hui-Tian Yang, Xiao-Mei Hu, Rong Hu, Xiao-Fen Zhan, Hui Yang, Zhao-Yun Luo, Wen-Yu Li, and Li-Ye Yang
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Medicine ,Science - Abstract
BACKGROUND: Thalassemia is the most common inherited disease in southern China. However, this disorder is usually ignored by Jiangxi provincial health system and government due to lack of epidemiological data. MATERIALS AND METHODS: A total of 9489 samples from Hakka Han and Gan-speaking Han in three geographical areas of Jiangxi Province were analyzed for both complete blood cell (CBC) count and reverse dot blot (RDB) gene chip for thalassemia. RESULTS: 1182 cases of suspected thalassemia carriers with microcytosis (MCV
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- 2014
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16. Pesticide exposure as a risk factor for myelodysplastic syndromes: a meta-analysis based on 1,942 cases and 5,359 controls.
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Jie Jin, Mengxia Yu, Chao Hu, Li Ye, Lili Xie, Jin Jin, Feifei Chen, and Hongyan Tong
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Medicine ,Science - Abstract
Pesticide exposure has been linked to increased risk of cancer at several sites, but its association with risk of myelodysplastic syndromes (MDS) is still unclear. A meta-analysis of studies published through April, 2014 was performed to investigate the association of pesticide exposure with the risk of MDS.Studies were identified by searching the Web of Science, Cochrane Library and PubMed databases. Summary odds ratios (ORs) with corresponding 95% confidence intervals (CIs) were calculated using random- or fixed-effect models.This meta-analysis included 11 case-control studies, all of which demonstrated a correlation between pesticide exposure and a statistically significant increased risk of MDS (OR=1.95, 95% CI 1.23-3.09). In subgroup analyses, patients with pesticide exposure had increased risk of developing MDS if they were living in the Europe or Asia and had refractory anemia (RA) or RA with ringed sideroblasts (RARS). Moreover, in the analysis by specific pesticides, increased risk was associated with exposure to insecticides (OR=1.71, 95% CI 1.22-2.40) but not exposure to herbicides or fungicides.This meta-analysis supports the hypothesis that exposure to pesticides increases the risk of developing MDS. Further prospective cohort studies are warranted to verify the association and guide clinical practice in MDS prevention.
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- 2014
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17. HIV pre-exposure prophylaxis interest among female sex workers in Guangxi, China.
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Li Ye, Suosu Wei, Yunfeng Zou, Xiaobo Yang, Abu S Abdullah, Xiaoni Zhong, Yuhua Ruan, Xinqin Lin, Mingqiang Li, Deren Wu, Junjun Jiang, Peiyan Xie, Jiegang Huang, Bingyu Liang, Bo Zhou, Jinming Su, Hao Liang, and Ailong Huang
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Medicine ,Science - Abstract
OBJECTIVES: Acceptability of pre-exposure prophylaxis (PrEP) and willingness to participate in a clinical trial for both safety and efficacy of PrEP were investigated among female sex workers (FSWs) in Guangxi, China. METHODS: A cross-sectional study was performed in three cities in Guangxi. Structured, self-administered questionnaires were used to assess the acceptability of PrEP and the willingness to participate in a clinical trial. Multivariable logistic regression models were fitted to identify predictors. RESULTS: Among 405 participants, 15.1% had heard of PrEP. If PrEP was deemed to be effective, safe and provided for free, 85.9% reported that they would accept it, and 54.3% of those who accepted PrEP said that they would participate in a clinical trial. The increased acceptability of PrEP was associated with working in male dominated venues, higher income, a poor family relationship, better HIV/AIDS knowledge, not realizing HIV risk from unfamiliar clients, not being forced to use condoms by the gatekeepers, consistent use of condoms, and use of drugs to prevent STD infection. The increased willingness to participate in a clinical trial was associated with a poor family relationship, better HIV/AIDS knowledge, not realizing HIV risk from unfamiliar clients, a willingness to adhere to daily PreP use, and not being concerned about discrimination by others. The main reason for rejecting PrEP or participating in a clinical trial was the concern about the side effects of PrEP. CONCLUSIONS: Acceptability of PrEP among Guangxi FSWs is relatively high, indicating that PrEP intervention programs may be feasible for Chinese FSWs. Given the fact that most of the participants had never heard of PrEP before, and that family, gatekeepers, and social discrimination could significantly affect its acceptability, a comprehensive mix of multiple interventions is necessary for the successful implementation of a PrEP program among this population in Guangxi.
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- 2014
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18. Repeatability of corneal and epithelial thickness measurements with anterior segment optical coherence tomography in keratoconus
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Li, Ye, primary, Gokul, Akilesh, additional, McGhee, Charles, additional, and Ziaei, Mohammed, additional
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- 2021
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19. Repeatability and agreement of biometric measurements using spectral domain anterior segment optical coherence tomography and Scheimpflug tomography in keratoconus
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Li, Ye, primary, Gokul, Akilesh, additional, McGhee, Charles, additional, and Ziaei, Mohammed, additional
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- 2021
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20. Combined abdominal heterotopic heart and aorta transplant model in mice
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Yuehui Zhu, Hao Dun, Li Ye, and Brian W. Wong
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Male ,Transplantation, Heterotopic ,Cardiovascular Procedures ,medicine.medical_treatment ,030204 cardiovascular system & hematology ,030230 surgery ,Mice ,0302 clinical medicine ,Medicine and Health Sciences ,Aorta, Abdominal ,Aorta ,Heart transplantation ,Mice, Inbred BALB C ,Multidisciplinary ,Abdominal aorta ,Drugs ,Heart ,Cardiac Transplantation ,Immunosuppressives ,surgical procedures, operative ,medicine.vein ,Abdominal Surgery ,cardiovascular system ,Medicine ,Female ,Anatomy ,Research Article ,medicine.medical_specialty ,Science ,Immunology ,Surgical and Invasive Medical Procedures ,Anastomosis ,Inferior vena cava ,03 medical and health sciences ,Transplantation Immunology ,medicine.artery ,medicine ,Animals ,Renal artery ,Ligature ,Survival rate ,Pharmacology ,Transplantation ,business.industry ,Transplant Rejection ,Biology and Life Sciences ,Organ Transplantation ,Surgery ,Cardiovascular Anatomy ,Heart Transplantation ,Blood Vessels ,Clinical Immunology ,Clinical Medicine ,business ,Abdominal surgery - Abstract
BackgroundAllograft vasculopathy (AV) remains a major obstacle to long-term allograft survival. While the mouse aortic transplantation model has been proven as a useful tool for study of the pathogenesis of AV, simultaneous transplantation of the aorta alongside the transplantation of another organ may reveal more clinically relevant mechanisms that contribute to the pathogenesis of chronic allograft rejection. Therefore, we developed a combined abdominal heart and aorta transplantation model in mice which benefits from reducing animal and drug utilization, while providing an improved model to study the progressive nature of AV.MethodsThe middle of the infrarenal aorta of the recipient mouse was ligatured between the renal artery and its bifurcation. Proximal and distal aortotomies were performed at this site above and below the ligature, respectively, for the subsequent anastomoses of the donor aorta and heart grafts to the recipient infrarenal aorta in an end-to-side fashion. The distal anastomotic site of the recipient infrarenal aorta was connected with the outlet of the donor aorta. Uniquely, the proximal anastomotic site on the recipient infrarenal aorta was shared to connect with both the inlet of the donor aorta and the inflow tract to the donor heart. The outflow tract from the donor heart was connected to the recipient inferior vena cava (IVC).ResultsThe median times for harvesting the heart graft, aorta graft, recipient preparation and anastomosis were 11.5, 8.0, 9.0 and 40.5 min, respectively, resulting in a total median ischemic time of 70 min. The surgery survival rate was more than 96% (29/30). Both the syngeneic C57Bl/6 aorta and heart grafts survived more than 90 days in 29 C57Bl/6 recipients. Further, Balb/c to C57Bl/6 allografts treated with anti-CD40L and CTLA4.Ig survived more than 90 days with a 100% (3/3) survival rate. (3/3).ConclusionsThis model is presented as a new tool for researchers to investigate transplant immunology and assess immunosuppressive strategies. It is possible to share a common anastomotic stoma on the recipient abdominal aorta to reconstruct both the aorta graft entrance and heart graft inflow tract. This allows for the study of allogeneic effects on both the aorta and heart from the same animal in a single survival surgery.
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- 2020
21. Morphine suppresses IFN signaling pathway and enhances AIDS virus infection.
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Yizhong Wang, Xu Wang, Li Ye, Jieliang Li, Li Song, Nilija Fulambarkar, and Wenzhe Ho
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Medicine ,Science - Abstract
Opioids exert a profound influence on immunomodulation and enhance HIV infection and replication. However, the mechanism(s) of their action remains to be determined. We thus investigated the impact of morphine on the intracellular innate antiviral immunity.Seven-day-cultured macrophages were infected with equal amounts of cell-free HIV Bal or SIV Delta(B670) for 2 h at 37°C after 24 h of treatment with or without morphine. Effect of morphine on HIV/SIV infection and replication was evaluated by HIV/SIV RT activity assay and indirect immunofluorescence for HIV p24 or SIV p28 antigen. The mRNA expression of cellular factors suppressed or induced by morphine treatment was analyzed by the real-time RT-PCR. We demonstrated that morphine treatment of human blood monocyte-derived macrophages significantly inhibited the expression of interferons (IFN-α, IFN-β and IFN-λ) and IFN-inducible genes (APOBEC3C/3F/3G and 3H). The further experiments showed that morphine suppressed the expression of several key elements (RIG-I and IRF-7) in IFN signaling pathway. In addition, morphine treatment induced the expression of suppressor of cytokine signaling protein-1, 2, 3 (SOCS-1, 2, 3) and protein inhibitors of activated STAT-1, 3, X, Y (PIAS-1, 3, X, Y), the key negative regulators of IFN signaling pathway.These findings indicate that morphine impairs intracellular innate antiviral mechanism(s) in macrophages, contributing to cell susceptibility to AIDS virus infection.
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- 2012
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22. IFN-λ3 inhibits HIV infection of macrophages through the JAK-STAT pathway.
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Man-Qing Liu, Dun-Jin Zhou, Xu Wang, Wang Zhou, Li Ye, Jie-Liang Li, Yi-Zhong Wang, and Wen-Zhe Ho
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Medicine ,Science - Abstract
BACKGROUND:Interferon lambda 3 (IFN-λ3) is a newly identified cytokine with antiviral activity, and its single nucleotide polymorphisms are strongly associated with the treatment effectiveness and development of chronic hepatitis C virus infection. We thus examined the potential of IFN-λ3 to inhibit HIV replication and the possible mechanisms of the anti-HIV action by IFN-λ3 in human macrophages. PRINCIPAL FINDINGS:Under different conditions (before, during, and after HIV infection), IFN-λ3 significantly inhibited viral replication in macrophages, which was associated with the induction of multiple antiviral cellular factors (ISG56, MxA, OAS-1, A3G/F and tetherin) and IFN regulatory factors (IRF-1, 3, 5, 7 and 9). This anti-HIV action of IFN-λ3 could be compromised by the JAK-STAT inhibitor. In addition, IFN-λ3 treatment of macrophages induced the expression of toll-like receptor 3 (TLR3) and two key adaptors (MyD88 and TRIF) in type I IFN pathway activation. However, HIV infection compromised IFN-λ3-mediated induction of the key elements in JAK-STAT signaling pathway. CONCLUSIONS:These data indicate that IFN-λ3 exerts its anti-HIV function by activating JAK-STAT pathway-mediated innate immunity in macrophages. Future in vivo studies are necessary in order to explore the potential for developing IFN-λ3-based therapy for HIV disease.
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- 2012
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23. Upregulation of SOCS-3 and PIAS-3 impairs IL-12-mediated interferon-gamma response in CD56 T cells in HCV-infected heroin users.
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Li Ye, Xu Wang, David S Metzger, Eric Riedel, Luis J Montaner, and Wenzhe Ho
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Medicine ,Science - Abstract
CD56(+) T cells are abundant in liver and play an important role in host innate immunity against viral infections, including hepatitis C virus (HCV) infection, a common infection among heroin abusers. We thus investigated the in vivo impact of heroin use or heroin use plus HCV infection on the CD56(+) T cell frequency and function.A total of 37 heroin users with (17) or without (20) HCV infection and 17 healthy subjects were included in the study. Although there was no significant difference in CD56(+) T cell frequency in PBMCs among three study groups, CD56(+) T cells isolated from the heroin users had significantly lower levels of constitutive interferon-gamma (IFN-gamma) expression than those from the normal subjects. In addition, when stimulated by interleukin (IL)-12, CD56(+) natural T cells from HCV-infected heroin users produced significantly lower levels of IFN-gamma than those from the normal subjects. This diminished ability to produce IFN-gamma by CD56(+) T cells was associated with the increased plasma HCV viral loads in the HCV-infected heroin users. Investigation of the mechanisms showed that although heroin use or heroin use plus HCV infection had little impact on the expression of the key positive regulators (IL-12 receptors, STAT-1, 3, 4, 5, JAK-2, and TYK-2) in IL-12 pathway, heroin use or heroin use plus HCV infection induced the expression of suppressor of cytokine signaling protein-3 (SOCS-3) and protein inhibitors of activated STAT-3 (PIAS-3), two key inhibitors of IL-12 pathway.These findings provide compelling in vivo evidence that heroin use or heroin use plus HCV infection impairs CD56(+) T cell-mediated innate immune function, which may account for HCV infection and persistence in liver.
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- 2010
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24. The effect of platelet-rich plasma on the fusion rate and clinical outcome of spinal fusion surgery: A systematic review and meta-analysis
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Cai, Ying-feng, primary, Tian, Tian-Zhao, additional, Chen, Li-Ye, additional, Liu, Bao-Xin, additional, Zhou, Jian-Peng, additional, Shi, Min, additional, and Liang, Hao-Dong, additional
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- 2020
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25. Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum
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Xiao-xue Lu, Jianping Xie, Yu-yang Feng, Xin-li Ye, Zhu-jun Zhang, Qian Li, Xuhu Mao, Guo-dong Dun, Ya-nan Tong, Bin Tang, and Dong-zhu Zeng
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0301 basic medicine ,Male ,Microarrays ,Cell Lines ,Artificial Gene Amplification and Extension ,Biochemistry ,Polymerase Chain Reaction ,Transcriptome ,0302 clinical medicine ,Medicine and Health Sciences ,Regulation of gene expression ,Multidisciplinary ,biology ,Gene Ontologies ,Genomics ,Middle Aged ,CREB-Binding Protein ,Enzymes ,Gene Expression Regulation, Neoplastic ,Nucleic acids ,Bioassays and Physiological Analysis ,Oncology ,030220 oncology & carcinogenesis ,Medicine ,Female ,Biological Cultures ,Colorectal Neoplasms ,Oxidoreductases ,Luciferase ,Signal Transduction ,Research Article ,Adult ,Science ,Research and Analysis Methods ,03 medical and health sciences ,Young Adult ,stomatognathic system ,microRNA ,medicine ,Genetics ,Humans ,CREB-binding protein ,Non-coding RNA ,Molecular Biology Techniques ,Molecular Biology ,Colorectal Cancer ,Natural antisense transcripts ,Fusobacterium nucleatum ,Biology and life sciences ,Microarray analysis techniques ,Cancer ,Cancers and Neoplasms ,Proteins ,Computational Biology ,Reverse Transcriptase-Polymerase Chain Reaction ,biology.organism_classification ,medicine.disease ,Genome Analysis ,Gene regulation ,MicroRNAs ,stomatognathic diseases ,030104 developmental biology ,Case-Control Studies ,biology.protein ,Gene chip analysis ,Cancer research ,Fusobacterium Infections ,Enzymology ,RNA ,Gene expression ,Caco-2 Cells - Abstract
Colorectal cancer (CRC) is a common and highly lethal form of cancer. Although the etiologic role of Fusobacterium nucleatum (F. nucleatum) in the development of CRC has been elucidated, the specific tumor molecules involved in the progression of CRC induced by F. nucleatum have not been identified. This study investigated several miRNAs and genes involved in the progression of F. nucleatum-induced CRC by Affymetrix miRNA microarray technology and GeneChip Human Transcriptome Array 2.0. The results suggest that miR-4474 and miR-4717 are up-regulated in CRC tissues in response to F. nucleatum infection, compared with the control group (paracancerous tissues), while other genes associated with signaling pathways in cancer, including CREB-binding protein (CREBBP), STAT1, PRKACB, CAMK2B, JUN, TP53 and EWSR1, were dysregulated. Bioinformatic analysis identified CREBBP as the primary aberrantly expressed gene in F. nucleatum-induced CRC. Consistent with the microarray analysis results, real-time RT-PCR analysis demonstrated that the expression of miR-4474/4717 was upregulated while that of CREBBP mRNA was downregulated in CRC patients infected with F. nucleatum. Additionally, CREBBP was identified as a novel target of miR-4474/4717. The results of this study suggest that miR-4474 and miR-4717 are involved in the progression of F. nucleatum-induced CRC by posttranscriptionally regulating the target gene CREBBP.
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- 2019
26. HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer
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Xue-Ying Jin, Jian-Fang Wang, Jian-Guo Zhao, Wan-Li Ye, and Jiang-Feng Feng
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Male ,0301 basic medicine ,Lung Neoplasms ,Databases, Factual ,Colorectal cancer ,Gene Expression ,Lung and Intrathoracic Tumors ,0302 clinical medicine ,Cell Signaling ,Cell Movement ,Carcinoma, Non-Small-Cell Lung ,Adenocarcinomas ,Gene expression ,Medicine and Health Sciences ,Membrane Glycoproteins ,Multidisciplinary ,Adenocarcinoma of the Lung ,Transfection ,Middle Aged ,Cancer Cell Migration ,Cell Motility ,Oncology ,030220 oncology & carcinogenesis ,Medicine ,Female ,Research Article ,Signal Transduction ,Science ,Down-Regulation ,Cell Migration ,Biology ,Carcinomas ,03 medical and health sciences ,Malignant Tumors ,Gentamicin protection assay ,Cell Line, Tumor ,Genetics ,medicine ,Adenocarcinoma of the lung ,Carcinoma ,Humans ,Lung cancer ,neoplasms ,Cell Proliferation ,Neoplasm Staging ,Cell growth ,Cancers and Neoplasms ,Biology and Life Sciences ,Cell Biology ,medicine.disease ,Non-Small Cell Lung Cancer ,respiratory tract diseases ,030104 developmental biology ,Hedgehog Signaling ,Cancer research ,Secondary Lung Tumors ,Carrier Proteins ,Developmental Biology - Abstract
ObjectiveThe primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).MethodsFirstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP.ResultsHHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (PConclusionOverall, the above results indicated that HHIP could regulate proliferation, migration and invasion, and could be used as a judging criterion for identifying NSCLC classification and stage.
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- 2019
27. Accuracy of rapid diagnosis of Talaromyces marneffei: A systematic review and meta-analysis
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Ning Zang, Chuanyi Ning, Cunwei Cao, Jingzhen Lai, Bo Zhou, Jiegang Huang, Hao Liang, Li Ye, Wudi Wei, Hui Chen, Bingyu Liang, Junjun Jiang, and Yanyan Liao
- Subjects
0301 basic medicine ,Pulmonology ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Cochrane Library ,Polymerase Chain Reaction ,Serology ,Mathematical and Statistical Techniques ,Medicine and Health Sciences ,Medicine ,Case Series ,Enzyme-Linked Immunoassays ,lcsh:Science ,Talaromyces marneffei ,Multidisciplinary ,Eukaryota ,Fungus culture ,Infectious Diseases ,Research Design ,Meta-analysis ,Cohort ,Physical Sciences ,Statistics (Mathematics) ,Research Article ,medicine.medical_specialty ,Clinical Research Design ,030106 microbiology ,MEDLINE ,Opportunistic Infections ,Research and Analysis Methods ,03 medical and health sciences ,Diagnostic Medicine ,Internal medicine ,Humans ,Serologic Tests ,Statistical Methods ,Molecular Biology Techniques ,Immunoassays ,Molecular Biology ,business.industry ,lcsh:R ,Organisms ,Fungi ,Biology and Life Sciences ,Serum samples ,Mycoses ,Talaromyces ,Respiratory Infections ,Immunologic Techniques ,lcsh:Q ,business ,Mathematics ,Meta-Analysis - Abstract
Background To examine the accuracy of Rapid Diagnosis of Talaromyces marneffei (RDTM) in order to improve diagnosis and treatment for clinical measures and reduce the mortality due to associated infections. Methods In this systematic review and meta-analysis, we screened PubMed, Ovid (Cochrane library) and Web of Science, Chinese database CNKI and Wanfang for articles published between 1956 and December, 2017. Data were taken from cross-sectional studies as well as from baseline measurements in longitudinal studies with clinical follow-up. Articles were excluded if they did not contain a cohort with T. marneffei and a control cohort or a cohort with standard fungus culture. Data were extracted by two authors and checked by three for accuracy. For quality assessment, modified QUADAS-2 criteria were used. Results The 26 included diagnostic studies enrolled 5,594 objectives in 632 patients with T. marneffei infections and 2,612 negative controls between 1996 and 2017 in Thailand, Vietnam and China. The total combined sensitivity and specificity of rapid diagnosis of T. marneffei was 0.82 (95% CI: 0.68–0.90) and 0.99 (95% CI: 0.98–1.00). According to the experimental method, the included studies can be divided into three subgroups, including PCR-based, ELISA-based and others. The results showed these three subgroups had a highly pooled specificity of 1.00 (95% CI: 0.99–1.00), 0.99 (0.98–1.00) and 0.97 (95% CI: 0.91–1.00), respectively, while combined sensitivity was 0.84 (95% CI: 0.37–0.98), 0.82 (95% CI: 0.64–0.92) and 0.77 (95% CI: 0.54–0.91), respectively. Conclusions Although serological methods with a high specificity is essential for potential rapid diagnostic, false-negative results can be obtained in the serum samples, there is no suitable rapid serological test to refer to as is the case with TM infection.
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- 2018
28. High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China
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Yin-zhuo Yan, Jie Xue, Tian Ge, Wan Zou, Yu-jie Wei, Wei Wang, Li-ye Wang, Wen-rui Ma, and Wu Yun
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0301 basic medicine ,Leaves ,Microorganism ,lcsh:Medicine ,Aureobasidium ,Wine ,Artificial Gene Amplification and Extension ,Plant Science ,Polymerase Chain Reaction ,Agricultural Soil Science ,Medicine and Health Sciences ,Vitis ,lcsh:Science ,Soil Microbiology ,education.field_of_study ,Multidisciplinary ,biology ,Ecology ,Alcoholic Beverages ,Plant Anatomy ,food and beverages ,Eukaryota ,High-Throughput Nucleotide Sequencing ,Agriculture ,Plants ,Fruit and Vegetable Juices ,Horticulture ,Pleosporaceae ,Soil microbiology ,Oenococcus ,Research Article ,China ,Grapes ,Ecological Metrics ,Population ,Microbial Consortia ,Soil Science ,Research and Analysis Methods ,Fruits ,Beverages ,03 medical and health sciences ,Food microbiology ,education ,Molecular Biology Techniques ,Molecular Biology ,Nutrition ,Bacteria ,lcsh:R ,fungi ,Ecology and Environmental Sciences ,Organisms ,Fungi ,Biology and Life Sciences ,Species Diversity ,biology.organism_classification ,Diet ,030104 developmental biology ,Food Microbiology ,lcsh:Q - Abstract
In this study Illumina MiSeq was performed to investigate microbial diversity in soil, leaves, grape, grape juice and wine. A total of 1,043,102 fungal Internal Transcribed Spacer (ITS) reads and 2,422,188 high quality bacterial 16S rDNA sequences were used for taxonomic classification, revealed five fungal and eight bacterial phyla. At the genus level, the dominant fungi were Ascomycota, Sordariales, Tetracladium and Geomyces in soil, Aureobasidium and Pleosporaceae in grapes leaves, Aureobasidium in grape and grape juice. The dominant bacteria were Kaistobacter, Arthrobacter, Skermanella and Sphingomonas in soil, Pseudomonas, Acinetobacter and Kaistobacter in grape and grapes leaves, and Oenococcus in grape juice and wine. Principal coordinate analysis showed structural separation between the composition of fungi and bacteria in all samples. This is the first study to understand microbiome population in soil, grape, grapes leaves, grape juice and wine in Xinjiang through High-throughput Sequencing and identify microorganisms like Saccharomyces cerevisiae and Oenococcus spp. that may contribute to the quality and flavor of wine.
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- 2018
29. Clinical manifestations of reported Lyme disease cases in Ontario, Canada: 2005–2014
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Johnson, Karen O., primary, Nelder, Mark P., additional, Russell, Curtis, additional, Li, Ye, additional, Badiani, Tina, additional, Sander, Beate, additional, Sider, Douglas, additional, and Patel, Samir N., additional
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- 2018
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30. Under-reporting of pertussis in Ontario: A Canadian Immunization Research Network (CIRN) study using capture-recapture
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Crowcroft, Natasha S., primary, Johnson, Caitlin, additional, Chen, Cynthia, additional, Li, Ye, additional, Marchand-Austin, Alex, additional, Bolotin, Shelly, additional, Schwartz, Kevin, additional, Deeks, Shelley L., additional, Jamieson, Frances, additional, Drews, Steven, additional, Russell, Margaret L., additional, Svenson, Lawrence W., additional, Simmonds, Kimberley, additional, Mahmud, Salaheddin M., additional, and Kwong, Jeffrey C., additional
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- 2018
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31. Impaired visual, working, and verbal memory in first-episode, drug-naive patients with major depressive disorder in a Chinese population
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Chen, Ce, primary, Jiang, Wen-hui, additional, Wang, Wei, additional, Ma, Xian-cang, additional, Li, Ye, additional, Wu, Jin, additional, Hashimoto, Kenji, additional, and Gao, Cheng-ge, additional
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- 2018
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32. High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China
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Wei, Yu-jie, primary, Wu, Yun, additional, Yan, Yin-zhuo, additional, Zou, Wan, additional, Xue, Jie, additional, Ma, Wen-rui, additional, Wang, Wei, additional, Tian, Ge, additional, and Wang, Li-ye, additional
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- 2018
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33. Modulation of the gut microbiota by the mixture of fish oil and krill oil in high-fat diet-induced obesity mice
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Cui, Chenxi, primary, Li, Yanyan, additional, Gao, Hang, additional, Zhang, Hongyan, additional, Han, Jiaojiao, additional, Zhang, Dijun, additional, Li, Ye, additional, Zhou, Jun, additional, Lu, Chenyang, additional, and Su, Xiurong, additional
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- 2017
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34. Application of a Combined Model with Autoregressive Integrated Moving Average (ARIMA) and Generalized Regression Neural Network (GRNN) in Forecasting Hepatitis Incidence in Heng County, China
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Li Ye, Jinming Su, Hao Liang, Ning Zang, Jingzhen Lai, Jiegang Huang, Jun Yu, Junjun Jiang, Lian Gao, Wudi Wei, Yanyan Liao, Fengxiang Qin, Bingyu Liang, and Hui Chen
- Subjects
0301 basic medicine ,Gastroenterology and hepatology ,lcsh:Medicine ,Residual ,Pathology and Laboratory Medicine ,Infographics ,Hepatitis ,Geographical Locations ,Mathematical and Statistical Techniques ,Statistics ,Hepatitis Viruses ,Medicine and Health Sciences ,Public and Occupational Health ,Autoregressive integrated moving average ,lcsh:Science ,Mathematics ,Multidisciplinary ,Artificial neural network ,Incidence ,White noise ,Hepatitis A ,Regression ,Infectious hepatitis ,Mean absolute percentage error ,Medical Microbiology ,Viral Pathogens ,Physical Sciences ,Viruses ,Infectious diseases ,Public Health ,Pathogens ,Graphs ,Smoothing ,Statistics (Mathematics) ,Research Article ,China ,Computer and Information Sciences ,Asia ,Mean squared error ,Infectious Disease Control ,Viral diseases ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Humans ,Statistical Methods ,Microbial Pathogens ,Liver diseases ,Data Visualization ,lcsh:R ,Organisms ,Biology and Life Sciences ,Models, Theoretical ,Health Care ,030104 developmental biology ,People and Places ,lcsh:Q ,Neural Networks, Computer ,Health Statistics ,Morbidity ,Forecasting - Abstract
Background Hepatitis is a serious public health problem with increasing cases and property damage in Heng County. It is necessary to develop a model to predict the hepatitis epidemic that could be useful for preventing this disease. Methods The autoregressive integrated moving average (ARIMA) model and the generalized regression neural network (GRNN) model were used to fit the incidence data from the Heng County CDC (Center for Disease Control and Prevention) from January 2005 to December 2012. Then, the ARIMA-GRNN hybrid model was developed. The incidence data from January 2013 to December 2013 were used to validate the models. Several parameters, including mean absolute error (MAE), root mean square error (RMSE), mean absolute percentage error (MAPE) and mean square error (MSE), were used to compare the performance among the three models. Results The morbidity of hepatitis from Jan 2005 to Dec 2012 has seasonal variation and slightly rising trend. The ARIMA(0,1,2)(1,1,1)12 model was the most appropriate one with the residual test showing a white noise sequence. The smoothing factor of the basic GRNN model and the combined model was 1.8 and 0.07, respectively. The four parameters of the hybrid model were lower than those of the two single models in the validation. The parameters values of the GRNN model were the lowest in the fitting of the three models. Conclusions The hybrid ARIMA-GRNN model showed better hepatitis incidence forecasting in Heng County than the single ARIMA model and the basic GRNN model. It is a potential decision-supportive tool for controlling hepatitis in Heng County.
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- 2016
35. The Intelligent Control System and Experiments for an Unmanned Wave Glider
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Liao, Yulei, primary, Wang, Leifeng, additional, Li, Yiming, additional, Li, Ye, additional, and Jiang, Quanquan, additional
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- 2016
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36. Recommendations for Updating T and N Staging Systems for Nasopharyngeal Carcinoma in the Era of Intensity-Modulated Radiotherapy
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Liang, Zhong-Guo, primary, Chen, Xiao-Qian, additional, Niu, Zhi-Jie, additional, Chen, Kai-Hua, additional, Li, Ling, additional, Qu, Song, additional, Su, Fang, additional, Zhao, Wei, additional, Li, Ye, additional, Pan, Xin-Bin, additional, and Zhu, Xiao-Dong, additional
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- 2016
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37. Patient Satisfaction with Hospital Inpatient Care: Effects of Trust, Medical Insurance and Perceived Quality of Care
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Shan, Linghan, primary, Li, Ye, additional, Ding, Ding, additional, Wu, Qunhong, additional, Liu, Chaojie, additional, Jiao, Mingli, additional, Hao, Yanhua, additional, Han, Yuzhen, additional, Gao, Lijun, additional, Hao, Jiejing, additional, Wang, Lan, additional, Xu, Weilan, additional, and Ren, Jiaojiao, additional
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- 2016
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38. Multiple Genetic Modifiers of Bilirubin Metabolism Involvement in Significant Neonatal Hyperbilirubinemia in Patients of Chinese Descent
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Qian Wang, Hui Yang, Li-Ye Yang, Min Lin, Xiang-bin Zheng, Lei Zheng, and Fen Lin
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Male ,China ,Oxidoreductases Acting on CH-CH Group Donors ,Glucuronosyltransferase ,Genotype ,lcsh:Medicine ,Physiology ,Organic Anion Transporters ,Gestational Age ,Organic Anion Transporters, Sodium-Independent ,Polymorphism, Single Nucleotide ,Solute Carrier Organic Anion Transporter Family Member 1B3 ,Asian People ,Gene Frequency ,Genetic variation ,Humans ,Genetic Predisposition to Disease ,Allele ,lcsh:Science ,Allele frequency ,Alleles ,Retrospective Studies ,Genetics ,Multidisciplinary ,biology ,Liver-Specific Organic Anion Transporter 1 ,lcsh:R ,Case-control study ,Infant, Newborn ,Heterozygote advantage ,Bilirubin ,Breast Feeding ,Haplotypes ,Case-Control Studies ,biology.protein ,lcsh:Q ,Female ,Gene-Environment Interaction ,Hyperbilirubinemia, Neonatal ,SLCO1B1 ,Breast feeding ,Heme Oxygenase-1 ,Research Article - Abstract
The potential for genetic variation to modulate neonatal hyperbilirubinemia risk is increasingly being recognized. A case-control study was designed to assess comprehensive contributions of the multiple genetic modifiers of bilirubin metabolism on significant neonatal hyperbilirubinemia in Chinese descendents. Eleven common mutations and polymorphisms across five bilirubin metabolism genes, namely those encoding UGT1A1, HMOX1, BLVRA, SLCO1B1 and SLCO1B3, were determined using the high resolution melt (HRM) assay or PCR-capillary electrophoresis analysis. A total of 129 hyperbilirubinemic infants and 108 control subjects were evaluated. Breastfeeding and the presence of the minor A allele of rs4148323 (UGTA*6) were correlated with an increased risk of hyperbilirubinemia (OR=2.17, P=0.02 for breastfeeding; OR=9.776, P=0.000 for UGTA*6 homozygote; OR=3.151, P=0.000 for UGTA*6 heterozygote); whereas, increasing gestational age and the presence of -TA7 repeat variant of UGT1A1 decreased the risk (OR=0.721, P=0.003 for gestational age; OR=0.313, P=0.002 for heterozygote TA6/TA7). In addition, the SLCO1B1 and SLCO1B3 polymorphisms also contributed to an increased risk of hyperbilirubinemia. This detailed analysis revealed the impact of multiple genetic modifiers on neonatal hyperbilirubinemia. This may support the use of genetic tests for clinical risk assessment. Furthermore, the established HRM assay can serve as an effective method for large-scale investigation.
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- 2015
39. The Roles of Endoplasmic Reticulum Overload Response Induced by HCV and NS4B Protein in Human Hepatocyte Viability and Virus Replication
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Jing Liu, Shanshan Li, Xiangdong Zhu, Ying Xiong, Qinghua Zhang, Ruina Sun, Lingbao Kong, Li Ye, Mingjie Huang, and Yunli Guo
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Gene Flow ,Cell Survival ,lcsh:Medicine ,Hepacivirus ,Biology ,Viral Nonstructural Proteins ,Endoplasmic Reticulum ,Virus Replication ,Cell Line ,Humans ,Viability assay ,lcsh:Science ,Transcription factor ,Multidisciplinary ,Endoplasmic reticulum ,lcsh:R ,NF-kappa B ,virus diseases ,NFKB1 ,Cell Transformation, Viral ,Molecular biology ,digestive system diseases ,Cell biology ,Viral replication ,Unfolded protein response ,Hepatocytes ,lcsh:Q ,Calcium ,Signal transduction ,ER overload response ,Reactive Oxygen Species ,Research Article ,Signal Transduction - Abstract
Hepatitis C virus (HCV) replication is associated with endoplasmic reticulum (ER) and its infection triggers ER stress. In response to ER stress, ER overload response (EOR) can be activated, which involves the release of Ca2+ from ER, production of reactive oxygen species (ROS) and activation of nuclear factor κB (NF-κB). We have previously reported that HCV NS4B expression activates NF-κB via EOR-Ca2+-ROS pathway. Here, we showed that NS4B expression and HCV infection activated cancer-related NF-κB signaling pathway and induced the expression of cancer-related NF-κB target genes via EOR-Ca2+-ROS pathway. Moreover, we found that HCV-activated EOR-Ca2+-ROS pathway had profound effects on host cell viability and HCV replication. HCV infection induced human hepatocyte death by EOR-Ca2+-ROS pathway, whereas activation of EOR-Ca2+-ROS-NF-κB pathway increased the cell viability. Meanwhile, EOR-Ca2+-ROS-NF-κB pathway inhibited acute HCV replication, which could alleviate the detrimental effect of HCV on cell viability and enhance chronic HCV infection. Together, our findings provide new insights into the functions of EOR-Ca2+-ROS-NF-κB pathway in natural HCV replication and pathogenesis.
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- 2015
40. Seroprevalence of Human Immunodeficiency Virus, Hepatitis B Virus, Hepatitis C Virus, and Treponema pallidum Infections among Blood Donors on Bioko Island, Equatorial Guinea
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Min Lin, Li-Ye Yang, Dong-De Xie, Jiang-Tao Chen, Urbano Monsuy Eyi, Maximo Miko Ondo Obono, Hui-Tian Yang, Rocio Apicante Matesa, Hui Yang, Carlos Sala Ehapo, and Jian Li
- Subjects
Adult ,Male ,Blood transfusion ,Adolescent ,Hepatitis C virus ,medicine.medical_treatment ,Population ,lcsh:Medicine ,Blood Donors ,HIV Infections ,medicine.disease_cause ,Young Adult ,Seroepidemiologic Studies ,medicine ,Prevalence ,Seroprevalence ,Humans ,Mass Screening ,Syphilis ,Treponema pallidum ,education ,lcsh:Science ,Mass screening ,Retrospective Studies ,Hepatitis B virus ,education.field_of_study ,Multidisciplinary ,business.industry ,lcsh:R ,virus diseases ,Hepatitis B ,Middle Aged ,medicine.disease ,Virology ,Equatorial Guinea ,Female ,lcsh:Q ,business ,Research Article - Abstract
Background Regular screening of transfusion-transmissible infections (TTIs), such as human immunodeficiency virus (HIV), hepatitis B and hepatitis C virus (HBV and HCV, respectively), and Treponema pallidum, in blood donors is essential to guaranteeing clinical transfusion safety. This study aimed to determine the seroprevalence of four TTIs among blood donors on Bioko Island, Equatorial Guinea (EG). Methods A retrospective survey of blood donors from January 2011 to April 2013 was conducted to assess the presence of HIV, HBV, HCV and T. pallidum. The medical records were analyzed to verify the seroprevalence of these TTIs among blood donations stratified by gender, age and geographical region. Results Of the total 2937 consecutive blood donors, 1098 (37.39%) had a minimum of one TTI and 185 (6.29%) harbored co-infections. The general seroprevalence of HIV, HBV, HCV and T. pallidum were 7.83%, 10.01%, 3.71% and 21.51%, respectively. The most frequent TTI co-infections were HBV-T. pallidum 60 (2.04%) and HIV-T. pallidum 46 (1.57%). The seroprevalence of HIV, HBV, HCV and T. pallidum were highest among blood donors 38 to 47 years, 18 to 27 years and ≥ 48 years age, respectively (P
- Published
- 2015
41. G6PD Deficiency and Hemoglobinopathies: Molecular Epidemiological Characteristics and Healthy Effects on Malaria Endemic Bioko Island, Equatorial Guinea
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Ji Dong Cheng, Hui Tian Yang, Min Lin, Dong De Xie, Rocio Apicante Matesa, Hui Yang, Juan Urbano Monsuy Eyi, Carlos Sala Ehapo, Li Ye Yang, Santiago-m Monte Nguba, Xiao Fen Zhan, Maximo Miko Ondo Obono, and Jiang Tao Chen
- Subjects
Adult ,Male ,Adolescent ,Endemic Diseases ,Anemia ,Population ,Hemoglobin, Sickle ,Physiology ,lcsh:Medicine ,Alpha-thalassemia ,Biology ,Glucosephosphate Dehydrogenase ,parasitic diseases ,medicine ,Humans ,education ,Child ,lcsh:Science ,Allele frequency ,Aged ,Newborn screening ,education.field_of_study ,Multidisciplinary ,lcsh:R ,Hemoglobin C ,Infant ,Middle Aged ,medicine.disease ,Malaria ,Hemoglobinopathies ,Glucosephosphate Dehydrogenase Deficiency ,Child, Preschool ,Immunology ,Equatorial Guinea ,Mutation ,Female ,lcsh:Q ,Glucose-6-phosphate dehydrogenase deficiency ,Research Article - Abstract
Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency and hemoglobinopathies were the inherited conditions found mostly in African. However, few epidemiological data of these disorders was reported in Equatorial Guinea (EQG). This study aimed to assess the prevalence and healthy effects of G6PD deficiency and hemoglobinopathies among the people on malaria endemic Bioko Island, EQG. Materials and Methods Blood samples from 4,144 unrelated subjects were analyzed for G6PD deficieny by fluorescence spot test (FST), high-resolution melting assay and PCR-DNA sequencing. In addition, 1,186 samples were randomly selected from the 4,144 subjects for detection of hemoglobin S (HbS), HbC, and α-thalassemia deletion by complete blood count, PCR-DNA sequencing and reverse dot blot (RDB). Results The prevalence of malaria and anemia was 12.6% (522/4,144) and 32.8% (389/1,186), respectively. Overall, 8.7% subjects (359/4,144) were G6PD-deficient by FST, including 9.0% (249/2,758) males and 7.9% (110/1,386) females. Among the 359 G6PD-deficient individuals molecularly studied, the G6PD A- (G202A/A376G) were detected in 356 cases (99.2%), G6PD Betica (T968C/A376G) in 3 cases. Among the 1,186 subjects, 201 cases were HbS heterozygotes, 35 cases were HbC heterozygotes, and 2 cases were HbCS double heterozygotes; 452 cases showed heterozygous α-thalassemia 3.7 kb deletion (-α3.7 kb deletion) and 85 homozygous - α3.7 kb deletion. The overall allele frequencies were HbS 17.1% (203/1186); HbC, 3.1% (37/1186); and –α3.7 kb deletion 52.4% (622/1186), respectively. Conclusions High G6PD deficiency in this population indicate that diagnosis and management of G6PD deficiency is necessary on Bioko Island. Obligatory newborn screening, prenatal screening and counseling for these genetic disorders, especially HbS, are needed on the island.
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- 2015
42. Acceptability of Male Circumcision among College Students in Medical Universities in Western China: A Cross-Sectional Study
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Qianqiu Wang, Bo Qin, Jinming Su, Li Ye, Chaohui Zhong, Bingyu Liang, Junjun Jiang, Wei Deng, Yuhua Ruan, Xiaobo Yang, Hao Liang, Ming-Bo Huang, Qian Wang, Halmurat Upur, and Jiegang Huang
- Subjects
Male ,Health Knowledge, Attitudes, Practice ,medicine.medical_specialty ,Universities ,Cross-sectional study ,media_common.quotation_subject ,Population ,lcsh:Medicine ,HIV Infections ,Logistic regression ,Acquired immunodeficiency syndrome (AIDS) ,Hygiene ,medicine ,Humans ,Students ,China ,education ,lcsh:Science ,media_common ,Gynecology ,education.field_of_study ,Multidisciplinary ,Cancer prevention ,business.industry ,lcsh:R ,medicine.disease ,Cross-Sectional Studies ,Circumcision, Male ,Male circumcision ,Family medicine ,Female ,lcsh:Q ,business ,Research Article - Abstract
Background Male circumcision (MC) has been shown to reduce the risk of female to male transmission of HIV. The goal of this survey was to explore MC's acceptability and the factors associated with MC among college students in medical universities in western China. Methods A cross-sectional study was carried out in three provinces in western China (Guangxi, Chongqing and Xinjiang) to assess the acceptability of MC as well as to discover factors associated with the acceptability among college students in medical universities. A total of 1,790 uncircumcised male students from three medical universities were enrolled in this study. In addition, 150 students who had undergone MC were also enrolled in the survey, and they participated in in-depth interviews. Results Of all the uncircumcised participants (n = 1,790), 55.2% (n = 988) were willing to accept MC. Among those who accepted MC, 67.3% thought that MC could improve their sexual partners' hygiene, 46.3% believed that HIV and sexually transmitted diseases (STDs) could be partially prevented by MC. The multivariable logistic regression indicates that MC's acceptability was associated with three factors: the redundant foreskin (OR = 10.171, 95% CI = 7.629-13.559), knowing the hazard of having a redundant foreskin (OR = 1.597, 95% CI = 1.097-2.323), and enhancing sexual pleasure (OR = 1.628, 95% CI = 1.312-2.021). The in-depth interviews for subjects who had undergone MC showed that the major reason for having MC was the redundant foreskin (87.3%), followed by the benefits and the fewer complications of having MC done. In addition, most of these participants (65.3%) said that the MC could enhance sexual satisfaction. Conclusions MC's acceptance among college students in medical universities is higher than it is among other populations in western China. An implementation of an MC programme among this population is feasible in the future.
- Published
- 2015
43. Differential induction of apoptosis and autophagy by pyrrolizidine alkaloid clivorine in human hepatoma Huh-7.5 cells and its toxic implication
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Hao Liang, Wenju Liu, Hui Chen, Shoucai Fang, Li Ye, Xu Li, Bo Zhou, Jun Tang, and Wen-Zhe Ho
- Subjects
0301 basic medicine ,Programmed cell death ,Carcinoma, Hepatocellular ,Pyrrolizidine alkaloid ,lcsh:Medicine ,Apoptosis ,03 medical and health sciences ,chemistry.chemical_compound ,Cell Line, Tumor ,Phagosomes ,Botany ,Autophagy ,Humans ,lcsh:Science ,Cytotoxicity ,Pyrrolizidine Alkaloids ,Multidisciplinary ,Cell growth ,Liver Neoplasms ,lcsh:R ,Cell biology ,030104 developmental biology ,chemistry ,Cell culture ,Hepatocytes ,lcsh:Q ,Senecionine ,Research Article - Abstract
Growing evidence suggests that the pyrrolizidine alkaloids (PAs)-induced hepatotoxicity is mediated by multiple cell death/defence modalities. However, the detailed mechanisms are still lacking. In this study, the hepatotoxic effects of four PAs including three retronecine-type ones (senecionine, seneciphylline and monocrotaline) and one otonecine-type (clivorine) on the proliferation of Huh-7.5 cells and the possible mechanisms were investigated. The results showed that all the PAs could inhibit cell proliferation and induce apoptosis in a concentration-dependent manner. Among them clivorine was the most significant one. In addition to its effect on apoptosis, clivorine treatment could promote autophagy in Huh-7.5 cells, as evidenced by the accumulation of autophagosomes, the enhancement of LC3B expression at the concentrations close to its IC0 value, and the increased conversion of LC3B-I to LC3B-II in the presence of lysosomal inhibitor (chloroquine) and decreased formation of green fluorescent protein (GFP)-LC3 positive puncta in the presence of autophagic sequestration inhibitor (3-methyladenine). Among the other tested PAs, senecionine and seneciphylline also activated autophagy at the same concentrations used for clivorine but monocrotaline did not. Furthermore, our study demonstrated that suppression or enhancement of autophagy resulted in the remarkable enhancement or suppression of senecionine, seneciphylline and clivorine-induced apoptosis at the concentration close to the IC10 for clivorine, respectively, indicating a protective role of autophagy against the PA-induced apoptosis at the low level of exposure. Collectively, our data suggest that PAs in different structures may exert different toxic disturbances on the liver cells. Apoptosis may be one of the most common models of the PA-induced cytotoxicity, while autophagy may be a structure-dependent defence model in the early stage of PA intoxication. Differential induction of apoptosis and autophagy probably depending on the concentration is essential for the cytotoxic potency of clivorine.
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- 2017
44. Pre-Exposure Prophylaxis for the Prevention of HIV Infection in High Risk Populations: A Meta-Analysis of Randomized Controlled Trials
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Chuanyi Ning, Jiegang Huang, Xiaoni Zhong, Hao Liang, Bingyu Liang, Li Ye, Ailong Huang, Hui Chen, Xiaoyi Yang, Ren-chuan Tao, Junjun Jiang, Cunwei Cao, and Bo Zhou
- Subjects
medicine.medical_specialty ,Drugs and Devices ,Drug Research and Development ,Clinical Research Design ,Anti-HIV Agents ,Science ,Overview ,HIV prevention ,MEDLINE ,Human immunodeficiency virus (HIV) ,HIV Infections ,Viral diseases ,medicine.disease_cause ,Global Health ,Men who have sex with men ,law.invention ,Pre-exposure prophylaxis ,Randomized controlled trial ,law ,Risk Factors ,Internal medicine ,Outcome Assessment, Health Care ,Medicine ,Humans ,Clinical Trials ,Hiv transmission ,Randomized Controlled Trials as Topic ,Multidisciplinary ,High risk populations ,business.industry ,virus diseases ,HIV ,Meta-analysis ,Infectious diseases ,Public Health ,Preventive Medicine ,Meta-Analyses ,business - Abstract
BackgroundNearly ten randomized controlled trials (RCTs) of pre-exposure prophylaxis (PrEP) have been completed or are ongoing worldwide to evaluate the effectiveness of PrEP in HIV transmission among HIV-uninfected high risk populations. The purpose of this study was to evaluate the role of PrEP to prevent HIV transmission through a Mata-analysis.MethodsA comprehensive computerized literature search was carried out in PubMed, EMbase, Ovid, Web of Science, Science Direct, Wan Fang, CNKI and related websites to collect relevant articles (from their establishment date to August 30, 2013). The search terms were "pre-exposure prophylaxis", "high risk population", "HIV infection", "reduction", "relative risk" and "efficacy". We included any RCT assessing PrEP for the prevention of HIV infection in high risk populations. Interventions of the studies were continuously daily or intermittent doses of single or compound antiretrovirals (ARVs) before HIV exposure or during HIV exposure. A meta-analysis was conducted using Stata 10.0. A random-effects method was used to calculate the pooled relative risk (RR) and 95% confidence intervals (CI) for all studies included.ResultsSeven RCTs involving 14,804 individuals in high risk populations were eligible for this study. The number of subjects in the experimental groups was 8,195, with HIV infection rate of 2.03%. The number of subjects in the control groups was 6,609, with HIV infection rate of 4.07%. The pooled RR was 0.53 (95% CI = 0.40 ∼ 0.71, PConclusionThese results show that PrEP is an effective strategy for reducing new HIV infections in high risk populations.
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- 2014
45. Low-temperature synthesis of anatase TiO2 nanoparticles with tunable surface charges for enhancing photocatalytic activity
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Tingying Zeng, Li Ye, Guojun Zhang, Shulan Ji, Qin Zhenping, Hongxia Guo, Hanxiao Yang, Massachusetts Institute of Technology. Research Laboratory of Electronics, and Zeng, Tingying
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Anatase ,Materials science ,Polymers ,Nanoparticle ,lcsh:Medicine ,Catalysis ,Ultraviolet visible spectroscopy ,Adsorption ,X-Ray Diffraction ,Industrial Engineering ,Polyethyleneimine ,Surface charge ,High-resolution transmission electron microscopy ,Coloring Agents ,lcsh:Science ,Titanium ,Multidisciplinary ,Photolysis ,lcsh:R ,Chemical Engineering ,Cold Temperature ,Chemical engineering ,Manufacturing Processes ,X-ray crystallography ,Photocatalysis ,Nanoparticles ,Engineering and Technology ,lcsh:Q ,Sulfonic Acids ,Research Article - Abstract
In this work, the positively or negatively charged anatase TiO[subscript 2] nanoparticles were synthesized via a low temperature precipitation-peptization process (LTPPP) in the presence of poly(ethyleneimine) (PEI) and poly(sodium4- styrenesulfonate) (PSS). X-ray diffraction (XRD) pattern and high-resolution transmission electron microscope (HRTEM) confirmed the anatase crystalline phase. The charges of the prepared TiO[subscript 2], PEI-TiO[subscript 2] and PSS-TiO[subscript 2] nanoparticles were investigated by zeta potentials. The results showed that the zeta potentials of PEI-TiO[subscript 2] nanoparticles can be tuned from +39.47 mV to +95.46 mV, and that of PSS-TiO[subscript 2] nanoparticles can be adjusted from −56.63 mV to −119.32 mV. In comparison with TiO[subscript 2], PSS-TiO[subscript 2] exhibited dramatic adsorption and degradation of dye molecules, while the PEI modified TiO[subscript 2] nanoparticles showed lower photocatalytic activity. The photocatalytic performances of these charged nanoparticles were elucidated by the results of UV-vis diffuse reflectance spectra (DRS) and the photoluminescence (PL) spectra, which indicated that the PSS-TiO[subscript 2] nanoparticles showed a lower recombination rate of electron-hole pairs than TiO[subscript 2] and PEI-TiO[subscript 2]., National Natural Science Foundation (China) (21176005)
- Published
- 2014
46. Molecular epidemiological characterization and health burden of thalassemia in Jiangxi Province, P. R. China
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Xin Tong, Fen Lin, Min Lin, Li-Ye Yang, Hui-Tian Yang, Xiao-Fen Zhan, Jiao-Ren Wu, Rong Hu, Xiao-Mei Hu, Wen-Yu Li, Hui Yang, Jian-Zhong Wang, Tianyu Zhong, Zhao-Yun Luo, and Yi-Guo Chen
- Subjects
Male ,Health Screening ,Spatial Epidemiology ,Epidemiology ,Thalassemia ,lcsh:Medicine ,Alpha-thalassemia ,Pediatrics ,Database and Informatics Methods ,Cost of Illness ,Animal Cells ,hemic and lymphatic diseases ,Molecular Cell Biology ,Medicine and Health Sciences ,Prevalence ,Medicine ,Public and Occupational Health ,lcsh:Science ,Genetics ,Molecular Epidemiology ,Multidisciplinary ,Obstetrics ,Child and Adolescent Health Policy ,Microcytosis ,Data Collection ,Child Health ,Beta thalassemia ,Hematology ,Middle Aged ,Healthy Volunteers ,3. Good health ,Health Education and Awareness ,Genetic Epidemiology ,Alpha-Thalassemia ,Epidemiological Methods and Statistics ,Female ,Cellular Types ,Genetic Dominance ,Research Article ,Adult ,medicine.medical_specialty ,China ,congenital, hereditary, and neonatal diseases and abnormalities ,Beta-Thalassemia ,Adolescent ,Prenatal diagnosis ,Health Informatics ,Research and Analysis Methods ,Young Adult ,Autosomal Recessive Diseases ,Hydrops fetalis ,parasitic diseases ,Humans ,Aged ,Blood Cells ,Autosomal Recessive Traits ,Health Care Policy ,Molecular epidemiology ,Population Biology ,business.industry ,lcsh:R ,Biology and Life Sciences ,Health Risk Analysis ,Human Genetics ,Cell Biology ,medicine.disease ,Blood Cell Count ,Health Care ,Hemoglobinopathies ,Mutation ,lcsh:Q ,business - Abstract
Background Thalassemia is the most common inherited disease in southern China. However, this disorder is usually ignored by Jiangxi provincial health system and government due to lack of epidemiological data. Materials and Methods A total of 9489 samples from Hakka Han and Gan-speaking Han in three geographical areas of Jiangxi Province were analyzed for both complete blood cell (CBC) count and reverse dot blot (RDB) gene chip for thalassemia. Results 1182 cases of suspected thalassemia carriers with microcytosis (MCV
- Published
- 2014
47. Prognostic value of isocitrate dehydrogenase mutations in myelodysplastic syndromes: a retrospective cohort study and meta-analysis
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Zhengping Zhuang, Xiufeng Yin, Mengxia Yu, Jie Jin, Hongyan Tong, Li Ye, Chao Hu, and Feifei Chen
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Adult ,Male ,Oncology ,medicine.medical_specialty ,Pathology ,IDH1 ,Adolescent ,Decitabine ,lcsh:Medicine ,Biology ,medicine.disease_cause ,IDH2 ,Hematologic Cancers and Related Disorders ,Young Adult ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Medicine and Health Sciences ,medicine ,Humans ,lcsh:Science ,Survival rate ,Aged ,Retrospective Studies ,Aged, 80 and over ,Mutation ,Multidisciplinary ,Myelodysplastic syndromes ,lcsh:R ,Hematology ,Middle Aged ,Prognosis ,medicine.disease ,Isocitrate Dehydrogenase ,Survival Rate ,Isocitrate dehydrogenase ,International Prognostic Scoring System ,Myelodysplastic Syndromes ,Female ,lcsh:Q ,Research Article ,Follow-Up Studies ,medicine.drug - Abstract
Background Recent genomic sequencing efforts have identified a number of recurrent mutations in myelodysplastic syndromes (MDS) that may contribute to disease progression and overall survival, including mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2). Methods Pretreatment bone marrow (BM) samples were acquired from mononuclear cells in 146 adult patients with de novo MDS from January 2006 to June 2013. Polymerase chain reaction (PCR) and direct sequencing were performed on exon 4 of IDH1/2 genes and mutation status was correlated with overall survival (OS) and leukemia-free survival (LFS). We then performed a meta-analysis combining previously published and current studies to explore the effect of IDH mutations on OS and LFS in MDS. Results In our study, somatic mutations of either IDH gene were discovered in 11 MDS patients (7.53%) and were significantly correlated with poorer OS (P = 0.007). IDH mutations were specifically associated with a poorer OS in the intermediate-1 risk group by the International Prognostic Scoring System (IPSS) (P = 0.039). In addition, we discovered decitabine achieved a better therapeutic effect compared to other treatments in IDH mutation-positive patients (P = 0.023). We identified six previous studies of IDH mutations in MDS. A meta-analysis of these studies included 111 MDS patients IDH mutations and 1671 MDS patients with wild-type IDH1/2. The hazard ratios (HRs) of OS and LFS for patients with IDH mutations were 1.62 (95% CI, 1.27-2.09) and 2.21 (95% CI, 1.48-3.30), respectively. Conclusion The results from our study and the meta-analysis provide firm evidence that IDH mutations are significantly associated with poorer clinical outcomes in MDS. Identification of IDH mutations may be pivotal for better risk stratification in MDS patients and improving IPSS score. Additionally, hypomethylating agents may be an effective treatment option for MDS patients with IDH mutations.
- Published
- 2014
48. Evaluating the Operational Features of an Unconventional Dual-Bay U-Turn Design for Intersections
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Xiang, Yun, primary, Li, Zhibin, additional, Wang, Wei, additional, Chen, Jingxu, additional, Wang, Hao, additional, and Li, Ye, additional
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- 2016
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49. Effect of Pioglitazone in Preventing In-Stent Restenosis after Percutaneous Coronary Intervention in Patients with Type 2 Diabetes: A Meta-Analysis
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Zhao, Shi-jie, primary, Zhong, Zhao-shuang, additional, Qi, Guo-xian, additional, Shi, Li-ye, additional, Chen, Ling, additional, and Tian, Wen, additional
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- 2016
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50. Genetic Divergence between Camellia sinensis and Its Wild Relatives Revealed via Genome-Wide SNPs from RAD Sequencing
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Yang, Hua, primary, Wei, Chao-Ling, additional, Liu, Hong-Wei, additional, Wu, Jun-Lan, additional, Li, Zheng-Guo, additional, Zhang, Liang, additional, Jian, Jian-Bo, additional, Li, Ye-Yun, additional, Tai, Yu-Ling, additional, Zhang, Jing, additional, Zhang, Zheng-Zhu, additional, Jiang, Chang-Jun, additional, Xia, Tao, additional, and Wan, Xiao-Chun, additional
- Published
- 2016
- Full Text
- View/download PDF
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