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18 results on '"Li Xiaoling"'

2. Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test

Author

Shao Yinxiu, Shulong Zheng, Li Xiaoling, Yong Qi, Rao Jixian, Chen Hongxia, Li Jiameng, Wenwen Zeng, Suyun Liu, Li Yuexi, Shen Wanpeng, and Yin Qiong

Subjects
Time Factors, Physiology, Rocky Mountain spotted fever, Rickettsia rickettsii, Recombinase Polymerase Amplification, lcsh:Medicine, Artificial Gene Amplification and Extension, Pathology and Laboratory Medicine, Biochemistry, Polymerase Chain Reaction, law.invention, Database and Informatics Methods, 0302 clinical medicine, law, Limit of Detection, Nucleic Acids, Medicine and Health Sciences, 030212 general & internal medicine, Rickettsia, lcsh:Science, Polymerase chain reaction, Multidisciplinary, Mammalian Genomics, biology, Chemistry, Genomics, Bacterial Pathogens, Body Fluids, Blood, Medical Microbiology, Pathogens, Anatomy, Nucleic Acid Amplification Techniques, Sequence Analysis, Research Article, DNA, Bacterial, Bioinformatics, 030231 tropical medicine, Research and Analysis Methods, Microbiology, Blood Plasma, Lateral flow test, Recombinases, 03 medical and health sciences, medicine, Genetics, Molecular Biology Techniques, Microbial Pathogens, Molecular Biology, DNA sequence analysis, Detection limit, Base Sequence, Bacteria, lcsh:R, Organisms, Biology and Life Sciences, DNA, medicine.disease, biology.organism_classification, Molecular biology, Spotted fever, Rickettsiosis, Animal Genomics, lcsh:Q, Sequence Alignment
Abstract

Objectives Rickettsia rickettsii is the causative agent of Rocky Mountain spotted fever, which is the most severe spotted fever group (SFG) rickettsiosis. Developing a simple and reliable detection method is required. Methods A detection method for R. rickettsii was established based on a recombinase polymerase amplification (RPA) assay and the lateral flow (LF) test. A specific target sequence was screened, and corresponding primers and probes were designed, synthesized, and screened for establishing an RPA assay with high amplification efficiency. Reagent concentrations, amplification time, and loading volume for strip development were optimized. The detection limit, analytic sensitivity and specificity were evaluated. Results A rapid, visual, sensitive and specific method for the detection of R. rickettsii based on RPA and the LF test was successfully established. The novel method had a limit of detection of 10 to 50 copies/reaction without recognizing other organisms. Analytical sensitivity and specificity were ≥90% and 100%, respectively, as evaluated by animal and simulative human samples. Conclusions Using the established method, detection could be completed in 30 min with visually detectable results by the naked eye, without requirement of any instrument except a constant temperature equipment. The technique shows superior detection performance and is promising for wide use in the field as well as resource-limited areas for R. rickettsii detection.

Published
2018

6. High Bak Expression Is Associated with a Favorable Prognosis in Breast Cancer and Sensitizes Breast Cancer Cells to Paclitaxel

Author

Luo, Yanwei, primary, Wang, Xinye, additional, Wang, Heran, additional, Xu, Yang, additional, Wen, Qiuyuan, additional, Fan, Songqing, additional, Zhao, Ran, additional, Jiang, Shihe, additional, Yang, Jing, additional, Liu, Yukun, additional, Li, Xiayu, additional, Xiong, Wei, additional, Ma, Jian, additional, Peng, Shuping, additional, Zeng, Zhaoyang, additional, Li, Xiaoling, additional, Phillips, Joshua B., additional, Li, Guiyuan, additional, Tan, Ming, additional, and Zhou, Ming, additional

Published
2015
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7. Burn Injury Alters the Intestinal Microbiome and Increases Gut Permeability and Bacterial Translocation

Author

Earley, Zachary M., primary, Akhtar, Suhail, additional, Green, Stefan J., additional, Naqib, Ankur, additional, Khan, Omair, additional, Cannon, Abigail R., additional, Hammer, Adam M., additional, Morris, Niya L., additional, Li, Xiaoling, additional, Eberhardt, Joshua M., additional, Gamelli, Richard L, additional, Kennedy, Richard H., additional, and Choudhry, Mashkoor A., additional

Published
2015
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8. LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2

Author

Gong, Zhaojian, primary, Zhang, Shanshan, additional, Zeng, Zhaoyang, additional, Wu, Hanjiang, additional, Yang, Qian, additional, Xiong, Fang, additional, Shi, Lei, additional, Yang, Jianbo, additional, Zhang, Wenling, additional, Zhou, Yanhong, additional, Zeng, Yong, additional, Li, Xiayu, additional, Xiang, Bo, additional, Peng, Shuping, additional, Zhou, Ming, additional, Li, Xiaoling, additional, Tan, Ming, additional, Li, Yong, additional, Xiong, Wei, additional, and Li, Guiyuan, additional

Published
2014
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10. Correction: LPLUNC1 Inhibits Nasopharyngeal Carcinoma Cell Growth via Down-Regulation of the MAP Kinase and Cyclin D1/E2F Pathways

Author

Yang, Yixin, primary, Liao, Qianjin, additional, Wei, Fang, additional, Li, Xiaoling, additional, Zhang, Wenling, additional, Fan, Songqing, additional, Shi, Lei, additional, Li, Xiayu, additional, Gong, Zhaojian, additional, Ma, Jian, additional, Zhou, Ming, additional, Xiang, Juanjuan, additional, Peng, Shuping, additional, Xiang, Bo, additional, Deng, Hao, additional, Yang, Yunbo, additional, Li, Yong, additional, Xiong, Wei, additional, Zeng, Zhaoyang, additional, and Li, Guiyuan, additional

Published
2013
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13. LPLUNC1 Inhibits Nasopharyngeal Carcinoma Cell Growth via Down-Regulation of the MAP Kinase and Cyclin D1/E2F Pathways

Author

Yang, Yixin, primary, Liao, Qianjin, additional, Wei, Fang, additional, Li, Xiaoling, additional, Zhang, Wenling, additional, Fan, Songqing, additional, Shi, Lei, additional, Li, Xiayu, additional, Gong, Zhaojian, additional, Ma, Jian, additional, Zhou, Ming, additional, Xiang, Juanjuan, additional, Peng, Shuping, additional, Xiang, Bo, additional, Deng, Hao, additional, Yang, Yunbo, additional, Li, Yong, additional, Xiong, Wei, additional, Zeng, Zhaoyang, additional, and Li, Guiyuan, additional

Published
2013
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14. SPLUNC1 Regulates Cell Progression and Apoptosis through the miR-141-PTEN/p27 Pathway, but Is Hindered by LMP1

Author

Chen, Pan, primary, Guo, Xiaofang, additional, Zhou, Houde, additional, Zhang, Wenling, additional, Zeng, Zhaoyang, additional, Liao, Qianjin, additional, Li, Xiayu, additional, Xiang, Bo, additional, Yang, Jianbo, additional, Ma, Jian, additional, Zhou, Ming, additional, Peng, Shuping, additional, Xiang, Juanjuan, additional, Li, Xiaoling, additional, LE, Colvin Wanshura, additional, Xiong, Wei, additional, McCarthy, James B., additional, and Li, Guiyuan, additional

Published
2013
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16. Circulating miR-17, miR-20a, miR-29c, and miR-223 Combined as Non-Invasive Biomarkers in Nasopharyngeal Carcinoma

Author

Zeng, Xi, primary, Xiang, Juanjuan, additional, Wu, Minghua, additional, Xiong, Wei, additional, Tang, Hailin, additional, Deng, Min, additional, Li, Xiayu, additional, Liao, Qianjin, additional, Su, Bo, additional, Luo, Zhaohui, additional, Zhou, Yanhong, additional, Zhou, Ming, additional, Zeng, Zhaoyang, additional, Li, Xiaoling, additional, Shen, Shourong, additional, Shuai, Cijun, additional, Li, Guiyuan, additional, Fang, Jiasheng, additional, and Peng, Shuping, additional

Published
2012
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18. Gene Polymorphisms of ADIPOQ +45T>G, UCP2 -866G>A, and FABP2 Ala54Thr on the Risk of Colorectal Cancer: A Matched Case-Control Study.

Author

Hu, Xiaoqin, Yuan, Ping, Yan, Jin, Feng, Fei, Li, Xiaoling, Liu, Wenhui, and Yang, Yanfang

Subjects
GENETIC polymorphisms, INSULIN resistance risk factors, COLON cancer, ADIPONECTIN, SINGLE nucleotide polymorphisms, LOGISTIC regression analysis, POPULATION genetics
Abstract

As insulin resistance (IR) is an established risk factor for colorectal cancer (CRC), we explored the association between each of the IR-related gene polymorphisms of adiponectin (ADIPOQ) rs2241766, uncoupling protein 2 (UCP2) rs659366, and fatty acid-binding protein (FABP2) rs1799883 and CRC risk. Genotyping of blood samples and collection of lifestyle and dietary habits were performed for 400 case-control pairs. Unconditional logistic regression (ULR) was applied to assess the effects of the three single nucleotide polymorphisms (SNP), environmental factors. Both ULR and generalized multifactor dimensionality reduction (GMDR) were used to test the gene-gene and gene-environment interactions on CRC risk. Subjects carrying the ADIPOQ rs2241766 TG+GG genotype had a higher CRC risk than those carrying the TT genotype (OR = 1.429, 95% CI 1.069–1.909). The additive and multiplicative interactions between ADIPOQ rs2241766 and FABP2 rs1799883 on CRC were found by ULR (RERI = 0.764, 95%CI 0.218∼1.311, AP = 0.514, 95%CI 0.165∼0.864, S = −1.745, 95%CI is unachievable, and Pmulti = 0.017, respectively). Furthermore, the high order gene-gene interaction of the three SNPs were found by GMDR (P = 0.0107). A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased (Ptrend = 0.037). In GMDR, the gene-environment interaction among the three SNPs and red meat consumption on CRC risk was significant (P = 0.0107). Compared with subjects with low red meat consumption and null risk genotypes, those with high-red meat consumption and three risk genotypes had 3.439-fold CRC risk (95% CI 1.410–8.385). In conclusion, the results showed that the ADIPOQ rs2241766 TG+GG genotype increased CRC risk. Given the complexity of the carcinogen for CRC, ADIPOQ rs2241766, UCP2 rs659366, FABP2 rs1799883 and red meat consumption potentially worked together in affecting CRC risk. [ABSTRACT FROM AUTHOR]

Published
2013
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