27 results on '"Liang Shi"'
Search Results
2. The safety and efficacy of hybrid ablation for the treatment of atrial fibrillation: A meta-analysis.
- Author
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Yun-Qiu Jiang, Ying Tian, Li-Jun Zeng, Shu-Nan He, Zhi-Tao Zheng, Liang Shi, Yan-Jiang Wang, Yu-Xing Wang, Xian-Dong Yin, Xiao-Qing Liu, Xin-Chun Yang, and Xing-Peng Liu
- Subjects
Medicine ,Science - Abstract
Hybrid ablation, an emerging therapy that combines surgical intervention and catheter ablation, has become a viable option for the treatment of persistent atrial fibrillation. In this analysis, we aimed to evaluate the safety and efficacy of hybrid ablation, as well as compare the outcomes of one-step and staged approaches.We conducted a search in major online databases and selected the studies that met the inclusion criteria. The primary endpoint was defined as no episode of atrial fibrillation or atrial tachycardia lasting longer than 30 seconds without administration of antiarrhythmic drugs.Sixteen studies including 785 patients (paroxysmal atrial fibrillation, n = 83; persistent atrial fibrillation, n = 214; long-standing persistent atrial fibrillation, n = 488) were selected. Average history of atrial fibrillation was (5.0±1.6) years. The pooled proportion of patients who were arrhythmia-free at the primary endpoint was 73% (95% CI, 64%-81%, Cochran's Q, P
- Published
- 2018
- Full Text
- View/download PDF
3. Helicobacter Pylori Promote B7-H1 Expression by Suppressing miR-152 and miR-200b in Gastric Cancer Cells.
- Author
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Gengchen Xie, Wei Li, Ruidong Li, Ke Wu, Ende Zhao, Yu Zhang, Peng Zhang, Liang Shi, Di Wang, Yuping Yin, Rui Deng, and Kaixiong Tao
- Subjects
Medicine ,Science - Abstract
The most common cause of gastric cancer is infection with helicobacter pylori (HP), but the associated molecular mechanism is not well understood. In the present study, we found a marked increase in the expression of B7-H1, a member of the B7 co-stimulatory family of molecules that bind to programmed death-1 (PD-1) and play a critical immunoregulatory role in the cell-mediated immune response, in HP-positive gastric cancer tissue. Infection of cultured gastric cancer cells with HP promoted B7-H1 expression and inhibited miR-152 and miR-200b expression. We further demonstrated that these two miRNAs targeted B7-H1 mRNA and suppressed B7-H1 expression in gastric cancer cells. Finally, B7-H1 expression was found to correlate with miR-152 and miR-200b levels in gastric tumor tissues from human patients. Our findings suggest a novel mechanism by which HP infection promotes gastric cancer and also suggest potential targets, i.e., miR-152 and miR-200b, for the prevention and treatment of gastric cancer.
- Published
- 2017
- Full Text
- View/download PDF
4. Different Stages, Different Signals: The Modulating Effect of Cognitive Conflict on Subsequent Processing.
- Author
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Fada Pan, Liang Shi, Li Zhang, Qingyun Lu, and Song Xue
- Subjects
Medicine ,Science - Abstract
The present study used event-related potentials (ERPs) to investigate the function of signals induced by cognitive conflict during the detection stage and the resolution stage of perceptual processing. The study used a combination of the Stroop task and an affective priming task to examine the conflict priming effect when the stimulus onset asynchrony (SOA) was 200 ms or 800 ms. Behavioral results showed that the RTs were shorter for positive targets following congruent primes relative to incongruent primes, and for negative targets following incongruent primes relative to congruent primes when the SOA was 200 ms. ERP results showed that the N2 amplitudes (200-300 ms) for incongruent stimuli were significantly larger than for congruent stimuli in the Stroop task, which indicated a significant conflict effect. Moreover, the N400 amplitudes (500-700 ms) for positive targets after congruent primes were significantly lower than those after incongruent primes when the SOA was 200 ms, which showed a significant negative priming effect. While the SOA was 800 ms, behavioral results showed that the RTs were shorter for positive targets following incongruent primes relative to congruent primes. ERP results showed that the N2 amplitudes (200-300 ms) for incongruent stimuli were significantly larger than for congruent stimuli in the Stroop task, which indicated a significant conflict effect. The N400 amplitudes (1100-1300 ms) for the negative targets after congruent primes were significantly lower than those after incongruent primes when the SOA was 800 ms, which showed a significant positive priming effect. The results demonstrated that the functions of signals induced by cognitive conflict were reversed in two different cognitive processing stages.
- Published
- 2016
- Full Text
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5. Prognostic Significance of the Metabolic Marker Hexokinase-2 in Various Solid Tumors: A Meta-Analysis.
- Author
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Yulin Liu, Ke Wu, Liang Shi, Fan Xiang, Kaixiong Tao, and Guobin Wang
- Subjects
Medicine ,Science - Abstract
Recently, numerous studies have reported that hexokinase-2 (HK2) is aberrantly expressed in cancer, indicating that HK2 plays a pivotal role in the development and progression of cancer. However, its prognostic significance in solid tumor remains unclear. Accordingly, we performed a meta-analysis to assess the prognostic value of HK2 in solid tumor.Eligible studies were identified using PubMed, Embase, and Web of Science databases. Pooled hazard ratios (HRs) with 95% confidence intervals (CIs) for overall survival (OS) or progression-free survival (PFS)/disease-free survival (DFS)/relapse-free survival (RFS) were estimated with random effects or fixed effects models, respectively. Subgroup analysis was also performed according to patients' ethnicities, tumor types, detection methods, and analysis types.Data from 21 included studies with 2532 patients were summarized. HK2 overexpression was significantly associated with worse OS (pooled HR = 1.90, 95% CI = 1.51-2.38, p < 0.001) and PFS (pooled HR = 2.91, 95% CI = 2.02-4.22, p < 0.001) in solid tumor. As to a specific form of cancer, the negative effect of HK2 on OS was observed in hepatocellular carcinoma (pooled HR = 2.06, 95% CI = 1.67-2.54, p < 0.001), gastric cancer (pooled HR = 1.72, 95% CI = 1.09-2.71, p = 0.020), colorectal cancer (pooled HR = 2.89, 95% CI = 1.62-5.16, p < 0.001), but not in pancreatic cancer (pooled HR = 1.13, 95% CI = 0.28-4.66, p = 0.864). No publication bias was found in the included studies for OS (Begg's test, p = 0.325; Egger's test, p = 0.441).In this meta-analysis, we identified that elevated HK2 expression was significantly associated with shorter OS and PFS in patients with solid tumor, but the association varies according to cancer type.
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- 2016
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6. Transcriptome analysis and its application in identifying genes associated with fruiting body development in basidiomycete Hypsizygus marmoreus.
- Author
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Jinjing Zhang, Ang Ren, Hui Chen, Mingwen Zhao, Liang Shi, Mingjie Chen, Hong Wang, and Zhiyong Feng
- Subjects
Medicine ,Science - Abstract
To elucidate the mechanisms of fruit body development in H. marmoreus, a total of 43609521 high-quality RNA-seq reads were obtained from four developmental stages, including the mycelial knot (H-M), mycelial pigmentation (H-V), primordium (H-P) and fruiting body (H-F) stages. These reads were assembled to obtain 40568 unigenes with an average length of 1074 bp. A total of 26800 (66.06%) unigenes were annotated and analyzed with the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Eukaryotic Orthologous Group (KOG) databases. Differentially expressed genes (DEGs) from the four transcriptomes were analyzed. The KEGG enrichment analysis revealed that the mycelium pigmentation stage was associated with the MAPK, cAMP, and blue light signal transduction pathways. In addition, expression of the two-component system members changed with the transition from H-M to H-V, suggesting that light affected the expression of genes related to fruit body initiation in H. marmoreus. During the transition from H-V to H-P, stress signals associated with MAPK, cAMP and ROS signals might be the most important inducers. Our data suggested that nitrogen starvation might be one of the most important factors in promoting fruit body maturation, and nitrogen metabolism and mTOR signaling pathway were associated with this process. In addition, 30 genes of interest were analyzed by quantitative real-time PCR to verify their expression profiles at the four developmental stages. This study advances our understanding of the molecular mechanism of fruiting body development in H. marmoreus by identifying a wealth of new genes that may play important roles in mushroom morphogenesis.
- Published
- 2015
- Full Text
- View/download PDF
7. The apoptotic effect of D Rhamnose β-hederin, a novel oleanane-type triterpenoid saponin on breast cancer cells.
- Author
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Lin Cheng, Tian-Song Xia, Yi-Fen Wang, Wenbin Zhou, Xiu-Qing Liang, Jin-Qiu Xue, Liang Shi, Ying Wang, and Qiang Ding
- Subjects
Medicine ,Science - Abstract
There is growing interest in development of natural products as anti-cancer and chemopreventive agents. Many triterpenoids have been proved as potential agents for chemoprevention and therapy of breast cancer. Ginsenosides from ginseng, which mostly belong to dammarane-type triterpenoids, have gained great attention for their anti-breast cancer activity with diverse mechanisms. However, studies of other kinds of triterpenoid saponins on breast cancer are limited. Previously, we purified and identified a novel oleanane-type triterpene saponin named D Rhamnose β-hederin (DRβ-H) from Clematis ganpiniana, a Chinese traditional anti-tumor herb. In the present study, DRβ-H showed strong inhibitory activity on the growth of various breast cancer cells and induced apoptosis in these cells. DRβ-H inhibited PI3K/AKT and activated ERK signaling pathway. PI3K inhibitor LY294002 synergistically enhanced DRβ-H-induced apoptosis whereas MEK inhibitor U0126 reduced the apoptosis rate. Moreover, DRβ-H regulated the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins. Furthermore, DRβ-H induced depolarization of mitochondrial membrane potential which released Apaf-1 and Cytochrome C from the inter membrane space into the cytosol, where they promoted caspase-9 and caspase-3 activation. This is the first report on the pro-apoptotic effects of DRβ-H, a novel oleanane-type triterpenoid saponin, on breast cancer cells and its comprehensive apoptosis pathways. It implied that oleanane-type triterpenoid saponin DRβ-H could be a promising candidate for chemotherapy of breast cancer.
- Published
- 2014
- Full Text
- View/download PDF
8. Correlates of 25-hydroxyvitamin D among Chinese breast cancer patients.
- Author
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Liang Shi, Sarah Nechuta, Yu-Tang Gao, Ying Zheng, Tsogzolmaa Dorjgochoo, Jie Wu, Qiuyin Cai, Wei Zheng, Wei Lu, and Xiao Ou Shu
- Subjects
Medicine ,Science - Abstract
Few studies have investigated vitamin D status in association with modifiable lifestyle factors and clinical characteristics among breast cancer patients, with no studies among Chinese women, who may be at higher risk of vitamin D deficiency. We aimed to evaluate circulating 25-hydroxyvitamin D (25(OH)D) levels in association with clinical and lifestyle factors among 1,940 Chinese breast cancer patients.Participants included breast cancer cases aged 22-77 from a population-based case-control study conducted in Shanghai, China during 1996-1998 (n = 1,044) and 2002-2005 (n = 896). Circulating 25(OH)D levels were measured in plasma samples (95% collected ≤6 months post-diagnosis). Prevalence ORs and 95% CIs were derived from multinomial logistic regression models, adjusting for age, season, and other factors.About 23% and 48% of women were vitamin D deficient (
- Published
- 2014
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9. Correction: Temporal Expression of Pelp1 during Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells.
- Author
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Jing Wang, Shujun Song, Liang Shi, Qiang Zhu, Chuanchuan Ma, Xiaoqing Tan, Yin Ding, and Zhongying Niu
- Subjects
Medicine ,Science - Published
- 2013
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10. Physical activity, smoking, and alcohol consumption in association with incidence of type 2 diabetes among middle-aged and elderly Chinese men.
- Author
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Liang Shi, Xiao-Ou Shu, Honglan Li, Hui Cai, Qiaolan Liu, Wei Zheng, Yong-Bing Xiang, and Raquel Villegas
- Subjects
Medicine ,Science - Abstract
Type 2 diabetes mellitus (T2DM) is a prevalent chronic disease worldwide. The prevalence of T2DM is increasing rapidly in China. Understanding the contribution of modifiable lifestyle factors on T2DM risk is imperative to prevent the development of T2DM in China.We examined associations between lifestyle factors including physical activity, smoking and alcohol consumption with incidence of T2DM among middle-aged and elderly men in urban Shanghai. Information on socio-demographics, lifestyle habits, dietary habits, and disease history was collected via in-person interviews. Anthropometric measurements were taken. A total of 51 464 Chinese men aged 40-74 years free of T2DM, coronary heart disease (CHD), and stroke at baseline were included in the current study. Incident T2DM was identified through follow-up surveys conducted every 2-3 years. Cox proportional hazard analyses were conducted to evaluate associations between lifestyle risk factors and incidence of T2DM.We documented 1304 new cases of T2DM during 276 929 person-years of follow-up (average: 5.4 years). Physical activity was inversely associated with T2DM risk. Daily living, commuting, and total physical activity METs had inverse negative dose-response relationships with T2DM (P-trend = 0.0033, 0.0022, and
- Published
- 2013
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11. Profiling and quantifying differential gene transcription provide insights into ganoderic acid biosynthesis in Ganoderma lucidum in response to methyl jasmonate.
- Author
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Ang Ren, Meng-Jiao Li, Liang Shi, Da-Shuai Mu, Ai-Liang Jiang, Qin Han, and Ming-Wen Zhao
- Subjects
Medicine ,Science - Abstract
Ganoderma lucidum is a mushroom with traditional medicinal properties that has been widely used in China and other countries in Eastern Asia. Ganoderic acids (GA) produced by G. lucidum exhibit important pharmacological activities. Previous studies have demonstrated that methyl jasmonate (MeJA) is a potent inducer of GA biosynthesis and the expression of genes involved in the GA biosynthesis pathway in G. lucidum. To further explore the mechanism of GA biosynthesis, cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) was used to identify genes that are differentially expressed in response to MeJA. Using 64 primer combinations, over 3910 transcriptionally derived fragments (TDFs) were obtained. Reliable sequence data were obtained for 390 of 458 selected TDFs. Ninety of these TDFs were annotated with known functions through BLASTX searching the GenBank database, and 12 annotated TDFs were assigned into secondary metabolic pathways by searching the KEGGPATHWAY database. Twenty-five TDFs were selected for qRT-PCR analysis to confirm the expression patterns observed with cDNA-AFLP. The qRT-PCR results were consistent with the altered patterns of gene expression revealed by the cDNA-AFLP technique. Additionally, the transcript levels of 10 genes were measured at the mycelium, primordia, and fruiting body developmental stages of G. lucidum. The greatest expression levels were reached during primordia for all of the genes except cytochrome b2 reached its highest expression level in the mycelium stage. This study not only identifies new candidate genes involved in the regulation of GA biosynthesis but also provides further insight into MeJA-induced gene expression and secondary metabolic response in G. lucidum.
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- 2013
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12. The development and application of a multiple gene co-silencing system using endogenous URA3 as a reporter gene in Ganoderma lucidum.
- Author
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Dashuai Mu, Liang Shi, Ang Ren, Mengjiao Li, Fengli Wu, Ailiang Jiang, and Mingwen Zhao
- Subjects
Medicine ,Science - Abstract
Ganoderma lucidum is one of the most important medicinal mushrooms; however, molecular genetics research on this species has been limited due to a lack of reliable reverse genetic tools. In this study, the endogenous orotidine 5'-monophosphate decarboxylase gene (URA3) was cloned as a silencing reporter, and four gene-silencing methods using hairpin, sense, antisense, and dual promoter constructs, were introduced into G. lucidum through a simple electroporation procedure. A comparison and evaluation of silencing efficiency demonstrated that all of the four methods differentially suppressed the expression of URA3. Our data unequivocally indicate that the dual promoter silencing vector yields the highest rate of URA3 silencing compared with other vectors (up to 81.9%). To highlight the advantages of the dual promoter system, we constructed a co-silencing system based on the dual promoter method and succeeded in co-silencing URA3 and laccase in G. lucidum. The reduction of the mRNA levels of the two genes were correlated. Thus, the screening efficiency for RNAi knockdown of multiple genes may be improved by the co-silencing of an endogenous reporter gene. The molecular tools developed in this study should facilitate the isolation of genes and the characterization of the functions of multiple genes in this pharmaceutically important species, and these tools should be highly useful for the study of other basidiomycetes.
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- 2012
- Full Text
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13. Biomechanics of the chick embryonic heart outflow tract at HH18 using 4D optical coherence tomography imaging and computational modeling.
- Author
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Aiping Liu, Xin Yin, Liang Shi, Peng Li, Kent L Thornburg, Ruikang Wang, and Sandra Rugonyi
- Subjects
Medicine ,Science - Abstract
During developmental stages, biomechanical stimuli on cardiac cells modulate genetic programs, and deviations from normal stimuli can lead to cardiac defects. Therefore, it is important to characterize normal cardiac biomechanical stimuli during early developmental stages. Using the chicken embryo model of cardiac development, we focused on characterizing biomechanical stimuli on the Hamburger-Hamilton (HH) 18 chick cardiac outflow tract (OFT), the distal portion of the heart from which a large portion of defects observed in humans originate. To characterize biomechanical stimuli in the OFT, we used a combination of in vivo optical coherence tomography (OCT) imaging, physiological measurements and computational fluid dynamics (CFD) modeling. We found that, at HH18, the proximal portion of the OFT wall undergoes larger circumferential strains than its distal portion, while the distal portion of the OFT wall undergoes larger wall stresses. Maximal wall shear stresses were generally found on the surface of endocardial cushions, which are protrusions of extracellular matrix onto the OFT lumen that later during development give rise to cardiac septa and valves. The non-uniform spatial and temporal distributions of stresses and strains in the OFT walls provide biomechanical cues to cardiac cells that likely aid in the extensive differential growth and remodeling patterns observed during normal development.
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- 2012
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14. Association between MLH1 -93G>a polymorphism and risk of colorectal cancer.
- Author
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Ting Wang, Yang Liu, Li Sima, Liang Shi, Zhaoming Wang, Chunhui Ni, Zhengdong Zhang, and Meilin Wang
- Subjects
Medicine ,Science - Abstract
The -93G>A (rs1800734) polymorphism located in the promoter of mismatch repair gene, MLH1, has been identified as a low-penetrance variant for cancer risk. Many published studies have evaluated the association between the MLH1 -93G>A polymorphism and colorectal cancer (CRC) risk. However, the results remain conflicting rather than conclusive.The aim of this study was to assess the association between the MLH1 -93G>A polymorphism and the risk of CRC.To derive a more precise estimation of the association, a meta-analysis of six studies (17,791 cases and 13,782 controls) was performed. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate the strength of the association. Four of these published studies were performed on subjects of known microsatellite instability (MSI) status. An additional analysis including 742 cases and 10,895 controls was used to assess the association between the MLH1 -93G>A polymorphism and the risk of MSI-CRC.The overall results indicated that the variant genotypes were associated with a significantly increased risk of CRC (AG versus GG: OR = 1.06, 95% CI = 1.01-1.11; AA/AG versus GG: OR = 1.06, 95% CI = 1.01-1.11). This increased risk was also found during stratified analysis of MSI status (AA versus GG: OR = 2.52, 95% CI = 1.94-3.28; AG versus GG: OR = 1.29, 95% CI = 1.10-1.52; AA/AG versus GG: OR = 1.45, 95% CI = 1.24-1.68; AA versusOR = 2.29, 95% CI = 1.78-2.96). Egger's test did not show any evidence of publication bias.Our results suggest that the MLH1 -93G>A polymorphism may contribute to individual susceptibility to CRC and act as a risk factor for MSI-CRC.
- Published
- 2012
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15. Risk factors for lactation mastitis in China: A systematic review and meta-analysis
- Author
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Lai, Bao-Yong, primary, Yu, Bo-Wen, additional, Chu, Ai-Jing, additional, Liang, Shi-Bing, additional, Jia, Li-Yan, additional, Liu, Jian-Ping, additional, Fan, Ying-Yi, additional, and Pei, Xiao-Hua, additional
- Published
- 2021
- Full Text
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16. A Bayesian network meta-analysis of the primary definitive therapies for locoregionally advanced nasopharyngeal carcinoma: IC+CCRT, CCRT+AC, and CCRT alone
- Author
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Zhan-Jie Zhang, Liang-Liang Shi, Xiao-Hua Hong, Bo-Ya Xiao, Guo-He Lin, Quentin Liu, and Bi-Cheng Wang
- Subjects
Nasopharyngeal Carcinoma ,Multidisciplinary ,Antineoplastic Combined Chemotherapy Protocols ,Network Meta-Analysis ,Humans ,Bayes Theorem ,Nasopharyngeal Neoplasms ,Chemoradiotherapy ,Induction Chemotherapy - Abstract
Background The major aim of this Bayesian network analysis was to determine the optimal treatment strategy for locoregionally advanced nasopharyngeal carcinoma (LANPC). Method We systematically searched databases and extracted data from randomized clinical trials involving LANPC patients randomly assigned to receive induction chemotherapy followed by concurrent chemoradiotherapy (IC+CCRT), CCRT followed by adjuvant chemotherapy (CCRT+AC), or CCRT. Results In the network analysis, IC+CCRT was significantly better than CCRT alone for 5-year FFS (odds ratio [OR]: 1.63, 95% credible interval [CrI] 1.16–2.29), DMFS (OR: 1.56, 95% CrI 1.08–2.22), and LFRS (OR: 1.62, 95% CrI 1.02–2.59), but not OS (OR: 1.35, 95% CrI 0.92–2.00). Rank probabilities showed that IC+CCRT was ranked the best followed by CCRT+AC and CCRT for all 5-year outcomes. Although compared to IC+CCRT and CCRT, CCRT+AC did not significantly improve survival but had the highest 5-year survival rates. Conclusions IC+CCRT could be recommended as a front-preferred primary definitive therapy for patients with LANPC.
- Published
- 2022
17. The safety and efficacy of hybrid ablation for the treatment of atrial fibrillation: A meta-analysis
- Author
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Xinchun Yang, Xiandong Yin, Xingpeng Liu, Xiaoqing Liu, Yun-Qiu Jiang, Shu-Nan He, Li-Jun Zeng, Yan-Jiang Wang, Liang Shi, Yuxing Wang, Zhi-Tao Zheng, and Ying Tian
- Subjects
Cardiovascular Procedures ,medicine.medical_treatment ,lcsh:Medicine ,030204 cardiovascular system & hematology ,law.invention ,Cohort Studies ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Randomized controlled trial ,law ,Atrial Fibrillation ,Clinical endpoint ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,Pharmaceutics ,Atrial fibrillation ,Research Assessment ,Ablation ,Research Design ,Meta-analysis ,Physical Sciences ,Cardiology ,Catheter Ablation ,medicine.symptom ,Open Heart Surgery ,Arrhythmia ,Statistics (Mathematics) ,Research Article ,Biotechnology ,medicine.medical_specialty ,Catheters ,Drug Administration ,Systematic Reviews ,Catheter ablation ,Surgical and Invasive Medical Procedures ,Research and Analysis Methods ,03 medical and health sciences ,Drug Therapy ,Internal medicine ,medicine ,Humans ,Statistical Methods ,Atrial tachycardia ,business.industry ,lcsh:R ,Biology and Life Sciences ,medicine.disease ,030228 respiratory system ,lcsh:Q ,Medical Devices and Equipment ,business ,Complication ,Mathematics ,Meta-Analysis - Abstract
Introduction Hybrid ablation, an emerging therapy that combines surgical intervention and catheter ablation, has become a viable option for the treatment of persistent atrial fibrillation. In this analysis, we aimed to evaluate the safety and efficacy of hybrid ablation, as well as compare the outcomes of one-step and staged approaches. Methods We conducted a search in major online databases and selected the studies that met the inclusion criteria. The primary endpoint was defined as no episode of atrial fibrillation or atrial tachycardia lasting longer than 30 seconds without administration of antiarrhythmic drugs. Results Sixteen studies including 785 patients (paroxysmal atrial fibrillation, n = 83; persistent atrial fibrillation, n = 214; long-standing persistent atrial fibrillation, n = 488) were selected. Average history of atrial fibrillation was (5.0±1.6) years. The pooled proportion of patients who were arrhythmia-free at the primary endpoint was 73% (95% CI, 64%–81%, Cochran’s Q, P
- Published
- 2017
18. Helicobacter Pylori Promote B7-H1 Expression by Suppressing miR-152 and miR-200b in Gastric Cancer Cells
- Author
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Ruidong Li, Yu Zhang, Yuping Yin, Ende Zhao, Di Wang, Peng Zhang, Gengchen Xie, Wei Li, Ke Wu, Liang Shi, Kaixiong Tao, and Rui Deng
- Subjects
0301 basic medicine ,Colorectal cancer ,Cancer Treatment ,Fluorescent Antibody Technique ,lcsh:Medicine ,Biochemistry ,B7-H1 Antigen ,White Blood Cells ,Prostate cancer ,Spectrum Analysis Techniques ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Gastrointestinal Infections ,lcsh:Science ,3' Untranslated Regions ,Immune Response ,Multidisciplinary ,biology ,T Cells ,Flow Cytometry ,Enzymes ,Nucleic acids ,Real-time polymerase chain reaction ,Oncology ,Spectrophotometry ,030220 oncology & carcinogenesis ,Cytophotometry ,Cellular Types ,Oxidoreductases ,Luciferase ,Research Article ,Immune Cells ,Immunology ,Gastroenterology and Hepatology ,Real-Time Polymerase Chain Reaction ,Research and Analysis Methods ,03 medical and health sciences ,Immune system ,Stomach Neoplasms ,Cell Line, Tumor ,Gastrointestinal Tumors ,microRNA ,Genetics ,medicine ,Humans ,Non-coding RNA ,Blood Cells ,Helicobacter pylori ,Biology and life sciences ,lcsh:R ,Cancers and Neoplasms ,Proteins ,Cancer ,Cell Biology ,biology.organism_classification ,medicine.disease ,Gene regulation ,MicroRNAs ,Gastric Cancer ,030104 developmental biology ,Cancer cell ,Enzymology ,Cancer research ,RNA ,lcsh:Q ,Gene expression - Abstract
The most common cause of gastric cancer is infection with helicobacter pylori (HP), but the associated molecular mechanism is not well understood. In the present study, we found a marked increase in the expression of B7-H1, a member of the B7 co-stimulatory family of molecules that bind to programmed death-1 (PD-1) and play a critical immunoregulatory role in the cell-mediated immune response, in HP-positive gastric cancer tissue. Infection of cultured gastric cancer cells with HP promoted B7-H1 expression and inhibited miR-152 and miR-200b expression. We further demonstrated that these two miRNAs targeted B7-H1 mRNA and suppressed B7-H1 expression in gastric cancer cells. Finally, B7-H1 expression was found to correlate with miR-152 and miR-200b levels in gastric tumor tissues from human patients. Our findings suggest a novel mechanism by which HP infection promotes gastric cancer and also suggest potential targets, i.e., miR-152 and miR-200b, for the prevention and treatment of gastric cancer.
- Published
- 2017
19. Risk of second breast cancers after lobular carcinoma in situ according to hormone receptor status
- Author
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Mao, Kai, primary, Yang, Yaping, additional, Wu, Wei, additional, Liang, Shi, additional, Deng, Heran, additional, and Liu, Jieqiong, additional
- Published
- 2017
- Full Text
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20. Transcriptome analysis and its application in identifying genes associated with fruiting body development in basidiomycete Hypsizygus marmoreus
- Author
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Ang Ren, Hong Wang, Mingwen Zhao, Zhiyong Feng, Chen Mingjie, Liang Shi, Hui Chen, and Zhang Jinjing
- Subjects
Nitrogen ,Sequence analysis ,Molecular Sequence Data ,lcsh:Medicine ,Biology ,Transcriptome ,Gene Expression Regulation, Plant ,Botany ,Gene expression ,Cyclic AMP ,Fruiting Bodies, Fungal ,KEGG ,DNA, Fungal ,lcsh:Science ,Gene ,Genetics ,Regulation of gene expression ,Multidisciplinary ,Base Sequence ,Mycelium ,Pigmentation ,Gene Expression Profiling ,TOR Serine-Threonine Kinases ,lcsh:R ,Fungal genetics ,Sequence Analysis, DNA ,Cryptochromes ,Gene expression profiling ,lcsh:Q ,Mitogen-Activated Protein Kinases ,Agaricales ,Reactive Oxygen Species ,Signal Transduction ,Research Article - Abstract
To elucidate the mechanisms of fruit body development in H. marmoreus, a total of 43609521 high-quality RNA-seq reads were obtained from four developmental stages, including the mycelial knot (H-M), mycelial pigmentation (H-V), primordium (H-P) and fruiting body (H-F) stages. These reads were assembled to obtain 40568 unigenes with an average length of 1074 bp. A total of 26800 (66.06%) unigenes were annotated and analyzed with the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Eukaryotic Orthologous Group (KOG) databases. Differentially expressed genes (DEGs) from the four transcriptomes were analyzed. The KEGG enrichment analysis revealed that the mycelium pigmentation stage was associated with the MAPK, cAMP, and blue light signal transduction pathways. In addition, expression of the two-component system members changed with the transition from H-M to H-V, suggesting that light affected the expression of genes related to fruit body initiation in H. marmoreus. During the transition from H-V to H-P, stress signals associated with MAPK, cAMP and ROS signals might be the most important inducers. Our data suggested that nitrogen starvation might be one of the most important factors in promoting fruit body maturation, and nitrogen metabolism and mTOR signaling pathway were associated with this process. In addition, 30 genes of interest were analyzed by quantitative real-time PCR to verify their expression profiles at the four developmental stages. This study advances our understanding of the molecular mechanism of fruiting body development in H. marmoreus by identifying a wealth of new genes that may play important roles in mushroom morphogenesis.
- Published
- 2015
21. Correlates of 25-hydroxyvitamin D among Chinese breast cancer patients
- Author
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Sarah Nechuta, Qiuyin Cai, Jie Wu, Wei Zheng, Wei Lu, Xiao-Ou Shu, Ying Zheng, Liang Shi, Tsogzolmaa Dorjgochoo, and Yu-Tang Gao
- Subjects
Epidemiology ,lcsh:Medicine ,Social and Behavioral Sciences ,Gastroenterology ,0302 clinical medicine ,Sociology ,030212 general & internal medicine ,Young adult ,Vitamin D ,lcsh:Science ,education.field_of_study ,Multidisciplinary ,Obstetrics and Gynecology ,Vitamins ,Middle Aged ,3. Good health ,Lifestyle factors ,Oncology ,030220 oncology & carcinogenesis ,Medicine ,Female ,Public Health ,Behavioral and Social Aspects of Health ,Research Article ,Adult ,medicine.medical_specialty ,China ,Clinical Research Design ,Population ,Physical activity ,Breast Neoplasms ,vitamin D deficiency ,03 medical and health sciences ,Young Adult ,Breast cancer ,Internal medicine ,Breast Cancer ,medicine ,Vitamin D and neurology ,Humans ,education ,Aged ,Nutrition ,Demography ,business.industry ,lcsh:R ,Case-control study ,Cancers and Neoplasms ,medicine.disease ,Vitamin D Deficiency ,Surgery ,Logistic Models ,Case-Control Studies ,Women's Health ,lcsh:Q ,business - Abstract
Background Few studies have investigated vitamin D status in association with modifiable lifestyle factors and clinical characteristics among breast cancer patients, with no studies among Chinese women, who may be at higher risk of vitamin D deficiency. We aimed to evaluate circulating 25-hydroxyvitamin D (25(OH)D) levels in association with clinical and lifestyle factors among 1,940 Chinese breast cancer patients. Methods Participants included breast cancer cases aged 22–77 from a population-based case-control study conducted in Shanghai, China during 1996–1998 (n = 1,044) and 2002–2005 (n = 896). Circulating 25(OH)D levels were measured in plasma samples (95% collected ≤6 months post-diagnosis). Prevalence ORs and 95% CIs were derived from multinomial logistic regression models, adjusting for age, season, and other factors. Results About 23% and 48% of women were vitamin D deficient (
- Published
- 2013
22. Temporal expression of Pelp1 during proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells
- Author
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Jing Wang, Qiang Zhu, Zhongying Niu, Chuanchuan Ma, Xiaoqing Tan, Yin Ding, Shujun Song, and Liang Shi
- Subjects
Time Factors ,medicine.drug_class ,Cellular differentiation ,Osteocalcin ,Estrogen receptor ,lcsh:Medicine ,Bone Marrow Cells ,Nerve Tissue Proteins ,Biology ,Real-Time Polymerase Chain Reaction ,Rats, Sprague-Dawley ,Osteogenesis ,medicine ,Animals ,RNA, Messenger ,RNA, Small Interfering ,lcsh:Science ,Cells, Cultured ,Cell Proliferation ,Regulation of gene expression ,Osteoblasts ,Multidisciplinary ,Cell growth ,Mesenchymal stem cell ,lcsh:R ,Nuclear Proteins ,Cell Differentiation ,Mesenchymal Stem Cells ,Transfection ,Alkaline Phosphatase ,Molecular biology ,Rats ,Cell biology ,Gene Expression Regulation ,Estrogen ,lcsh:Q ,Signal transduction ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,Signal Transduction - Abstract
BACKGROUND: Osteogenic induction and bone formation are heavily affected by environmental factors, including estrogen, estrogen receptors, and coregulatory proteins, such as the recently reported proline-, glutamic acid-, and leucine-rich protein 1(Pelp1). OBJECTIVE: To investigate Pelp1 expression in rat bone mesenchymal stem cells (rBMSCs) during cell proliferation and osteogenic differentiation. METHODS: rBMSCs were cultured in routine and osteogenic differentiation media. Cell proliferation was assessed at days 1, 3, 5, 7, 9, 11, 14, and 21. Pelp1 protein expression in the nucleus and cytoplasm were detected by immunocytochemical analysis. Real-time RT-PCR and western blot were used to detect mRNA and protein expressions of Pelp1, osteocalcin (OCN), and alkaline phosphatase (ALP). RESULTS: Over 21 days, rBMSCs in routine culture exhibited a 1-2 day lag phase and exponential growth from day 3 to 9, plateauing at day 9, and correlated with temporal mRNA expression of Pelp1, which almost reached baseline levels at day 21. In osteogenic induction cultures, Pelp1 mRNA levels rose at day 9 and steadily increased until day 21, reaching 6.8-fold greater value compared with day 1. Interestingly, Pelp1 mRNA expression in osteogenic cultures exhibited a trend similar to that of OCN expression. Pelp1 knockdown by siRNA transfection inhibited undifferentiated rBMSC proliferation, and bone markers OCN and ALP expressions in rBMSCs cultured in routine and osteogenic differentiation media. CONCLUSIONS: Pelp1 may be a key player in BMSCs proliferation and osteogenic differentiation, meriting further consideration as a target for development of therapies for pathological bone loss conditions, such as menopausal bone loss.
- Published
- 2013
23. Different Stages, Different Signals: The Modulating Effect of Cognitive Conflict on Subsequent Processing
- Author
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Qingyun Lu, Li Zhang, Liang Shi, Fada Pan, and Song Xue
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Male ,Physiology ,lcsh:Medicine ,Social Sciences ,Event-Related Potentials ,Electroencephalography ,Audiology ,Cognition ,Mathematical and Statistical Techniques ,0302 clinical medicine ,Medicine and Health Sciences ,Psychology ,lcsh:Science ,Evoked Potentials ,Clinical Neurophysiology ,Brain Mapping ,Multidisciplinary ,medicine.diagnostic_test ,05 social sciences ,Electrophysiology ,Bioassays and Physiological Analysis ,Brain Electrophysiology ,Physical Sciences ,Negative priming ,Engineering and Technology ,Female ,Analysis of variance ,Statistics (Mathematics) ,psychological phenomena and processes ,Research Article ,Adult ,medicine.medical_specialty ,Imaging Techniques ,Neurophysiology ,Neuroimaging ,Research and Analysis Methods ,behavioral disciplines and activities ,050105 experimental psychology ,Young Adult ,03 medical and health sciences ,Diagnostic Medicine ,Event-related potential ,medicine ,Humans ,0501 psychology and cognitive sciences ,Statistical Methods ,Analysis of Variance ,lcsh:R ,Electrophysiological Techniques ,Cognitive Psychology ,Biology and Life Sciences ,Priming (Psychology) ,Stimulus onset asynchrony ,N400 ,Signal Processing ,Stroop Test ,Cognitive Science ,lcsh:Q ,Perception ,Mathematics ,030217 neurology & neurosurgery ,Neuroscience ,Forecasting ,Stroop effect - Abstract
The present study used event-related potentials (ERPs) to investigate the function of signals induced by cognitive conflict during the detection stage and the resolution stage of perceptual processing. The study used a combination of the Stroop task and an affective priming task to examine the conflict priming effect when the stimulus onset asynchrony (SOA) was 200 ms or 800 ms. Behavioral results showed that the RTs were shorter for positive targets following congruent primes relative to incongruent primes, and for negative targets following incongruent primes relative to congruent primes when the SOA was 200 ms. ERP results showed that the N2 amplitudes (200-300 ms) for incongruent stimuli were significantly larger than for congruent stimuli in the Stroop task, which indicated a significant conflict effect. Moreover, the N400 amplitudes (500-700 ms) for positive targets after congruent primes were significantly lower than those after incongruent primes when the SOA was 200 ms, which showed a significant negative priming effect. While the SOA was 800 ms, behavioral results showed that the RTs were shorter for positive targets following incongruent primes relative to congruent primes. ERP results showed that the N2 amplitudes (200-300 ms) for incongruent stimuli were significantly larger than for congruent stimuli in the Stroop task, which indicated a significant conflict effect. The N400 amplitudes (1100-1300 ms) for the negative targets after congruent primes were significantly lower than those after incongruent primes when the SOA was 800 ms, which showed a significant positive priming effect. The results demonstrated that the functions of signals induced by cognitive conflict were reversed in two different cognitive processing stages.
- Published
- 2016
24. Biomechanics of the Chick Embryonic Heart Outflow Tract at HH18 Using 4D Optical Coherence Tomography Imaging and Computational Modeling
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Xin Yin, Liang Shi, Sandra Rugonyi, Kent L. Thornburg, Peng Li, Ruikang K. Wang, and Aiping Liu
- Subjects
Time Factors ,Blood Pressure ,Chick Embryo ,030204 cardiovascular system & hematology ,Cardiovascular ,Extracellular matrix ,0302 clinical medicine ,Biological Fluid Mechanics ,Morphogenesis ,Biomechanics ,0303 health sciences ,Numerical Analysis ,Multidisciplinary ,medicine.diagnostic_test ,Embryonic heart ,Applied Mathematics ,Physics ,Uncertainty ,Heart ,Anatomy ,Animal Models ,Chicken ,Biomechanical Phenomena ,Blood Circulation ,Heart Development ,Medicine ,Blood Flow Velocity ,Tomography, Optical Coherence ,Lumen (unit) ,Research Article ,Computer Modeling ,Science ,Movement ,Finite Element Analysis ,Biophysics ,03 medical and health sciences ,Model Organisms ,Optical coherence tomography ,medicine ,Animals ,Computer Simulation ,Biology ,Endocardium ,030304 developmental biology ,Mechanical Phenomena ,Myocardium ,Hemodynamics ,Blood flow ,Computer Science ,Stress, Mechanical ,Mathematics ,Developmental Biology - Abstract
During developmental stages, biomechanical stimuli on cardiac cells modulate genetic programs, and deviations from normal stimuli can lead to cardiac defects. Therefore, it is important to characterize normal cardiac biomechanical stimuli during early developmental stages. Using the chicken embryo model of cardiac development, we focused on characterizing biomechanical stimuli on the Hamburger-Hamilton (HH) 18 chick cardiac outflow tract (OFT), the distal portion of the heart from which a large portion of defects observed in humans originate. To characterize biomechanical stimuli in the OFT, we used a combination of in vivo optical coherence tomography (OCT) imaging, physiological measurements and computational fluid dynamics (CFD) modeling. We found that, at HH18, the proximal portion of the OFT wall undergoes larger circumferential strains than its distal portion, while the distal portion of the OFT wall undergoes larger wall stresses. Maximal wall shear stresses were generally found on the surface of endocardial cushions, which are protrusions of extracellular matrix onto the OFT lumen that later during development give rise to cardiac septa and valves. The non-uniform spatial and temporal distributions of stresses and strains in the OFT walls provide biomechanical cues to cardiac cells that likely aid in the extensive differential growth and remodeling patterns observed during normal development.
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- 2012
25. The Apoptotic Effect of D Rhamnose β-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells
- Author
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Liang Shi, Lin Cheng, Yi-Fen Wang, Ying Wang, Tiansong Xia, Xiuqing Liang, Qiang Ding, Wenbin Zhou, and Jinqiu Xue
- Subjects
Cancer Treatment ,Saponin ,lcsh:Medicine ,Apoptosis ,Phosphatidylinositol 3-Kinases ,chemistry.chemical_compound ,Triterpene ,Molecular Cell Biology ,Drug Discovery ,Breast Tumors ,LY294002 ,lcsh:Science ,Extracellular Signal-Regulated MAP Kinases ,Apoptotic Signaling Cascade ,Membrane Potential, Mitochondrial ,chemistry.chemical_classification ,Multidisciplinary ,Cell Death ,Caspase 3 ,Cytochromes c ,Drug Synergism ,Signaling in Selected Disciplines ,Caspase 9 ,Signaling Cascades ,Proto-Oncogene Proteins c-bcl-2 ,Oncology ,Biochemistry ,MCF-7 Cells ,Medicine ,Female ,Research Article ,Signal Transduction ,Drugs and Devices ,Drug Research and Development ,MAP Kinase Signaling System ,Morpholines ,Antineoplastic Agents ,Breast Neoplasms ,Biology ,Breast cancer ,Complementary and Alternative Medicine ,medicine ,Humans ,Oleanolic Acid ,PI3K/AKT/mTOR pathway ,Triterpenoid saponin ,Oncogenic Signaling ,lcsh:R ,Cancers and Neoplasms ,Cancer ,Saponins ,Chemotherapy and Drug Treatment ,medicine.disease ,Apoptotic Protease-Activating Factor 1 ,chemistry ,Chromones ,Cancer research ,lcsh:Q ,Drug Screening Assays, Antitumor ,Proto-Oncogene Proteins c-akt - Abstract
There is growing interest in development of natural products as anti-cancer and chemopreventive agents. Many triterpenoids have been proved as potential agents for chemoprevention and therapy of breast cancer. Ginsenosides from ginseng, which mostly belong to dammarane-type triterpenoids, have gained great attention for their anti-breast cancer activity with diverse mechanisms. However, studies of other kinds of triterpenoid saponins on breast cancer are limited. Previously, we purified and identified a novel oleanane-type triterpene saponin named D Rhamnose beta-hederin (DR beta-H) from Clematis ganpiniana, a Chinese traditional anti-tumor herb. In the present study, DR beta-H showed strong inhibitory activity on the growth of various breast cancer cells and induced apoptosis in these cells. DR beta-H inhibited PI3K/AKT and activated ERK signaling pathway. PI3K inhibitor LY294002 synergistically enhanced DR beta-H-induced apoptosis whereas MEK inhibitor U0126 reduced the apoptosis rate. Moreover, DR beta-H regulated the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins. Furthermore, DR beta-H induced depolarization of mitochondrial membrane potential which released Apaf-1 and Cytochrome C from the inter membrane space into the cytosol, where they promoted caspase-9 and caspase-3 activation. This is the first report on the pro-apoptotic effects of DR beta-H, a novel oleanane-type triterpenoid saponin, on breast cancer cells and its comprehensive apoptosis pathways. It implied that oleanane-type triterpenoid saponin DR beta-H could be a promising candidate for chemotherapy of breast cancer.
- Published
- 2014
26. The Development and Application of a Multiple Gene Co-Silencing System Using Endogenous URA3 as a Reporter Gene in Ganoderma lucidum
- Author
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Fengli Wu, Ai-Liang Jiang, Mingwen Zhao, Liang Shi, Ang Ren, Da-Shuai Mu, and Mengjiao Li
- Subjects
medicine.medical_specialty ,Reishi ,Science ,Applied Microbiology ,Molecular Sequence Data ,Orotidine-5'-Phosphate Decarboxylase ,Mycology ,Biology ,Microbiology ,RNA interference ,Genes, Reporter ,Molecular genetics ,Sense (molecular biology) ,Genetics ,medicine ,Gene silencing ,URA3 ,Gene Silencing ,RNA, Small Interfering ,Gene ,Gene knockdown ,Reporter gene ,Multidisciplinary ,Base Sequence ,Fungi ,Cell biology ,Medicine ,Epigenetics ,Gene Function ,Research Article ,Biotechnology - Abstract
Ganoderma lucidum is one of the most important medicinal mushrooms; however, molecular genetics research on this species has been limited due to a lack of reliable reverse genetic tools. In this study, the endogenous orotidine 5'-monophosphate decarboxylase gene (URA3) was cloned as a silencing reporter, and four gene-silencing methods using hairpin, sense, antisense, and dual promoter constructs, were introduced into G. lucidum through a simple electroporation procedure. A comparison and evaluation of silencing efficiency demonstrated that all of the four methods differentially suppressed the expression of URA3. Our data unequivocally indicate that the dual promoter silencing vector yields the highest rate of URA3 silencing compared with other vectors (up to 81.9%). To highlight the advantages of the dual promoter system, we constructed a co-silencing system based on the dual promoter method and succeeded in co-silencing URA3 and laccase in G. lucidum. The reduction of the mRNA levels of the two genes were correlated. Thus, the screening efficiency for RNAi knockdown of multiple genes may be improved by the co-silencing of an endogenous reporter gene. The molecular tools developed in this study should facilitate the isolation of genes and the characterization of the functions of multiple genes in this pharmaceutically important species, and these tools should be highly useful for the study of other basidiomycetes.
- Published
- 2012
27. The Development and Application of a Multiple Gene Co-Silencing System Using Endogenous URA3as a Reporter Gene in Ganoderma lucidum.
- Author
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Dashuai Mu, Liang Shi, Ang Ren, Mengjiao Li, Fengli Wu, Ailiang Jiang, Mingwen Zhao, and Tulane, Arthur J.
- Subjects
GANODERMA lucidum ,GENETIC research ,DECARBOXYLASES ,GENES ,MESSENGER RNA ,MOLECULAR genetics ,BASIDIOMYCETES - Abstract
Ganoderma lucidum is one of the most important medicinal mushrooms; however, molecular genetics research on this species has been limited due to a lack of reliable reverse genetic tools. In this study, the endogenous orotidine 5'-monophosphate decarboxylase gene (URA3) was cloned as a silencing reporter, and four gene-silencing methods using hairpin, sense, antisense, and dual promoter constructs, were introduced into G. lucidum through a simple electroporation procedure. A comparison and evaluation of silencing efficiency demonstrated that all of the four methods differentially suppressed the expression of URA3. Our data unequivocally indicate that the dual promoter silencing vector yields the highest rate of URA3 silencing compared with other vectors (up to 81.9%). To highlight the advantages of the dual promoter system, we constructed a co-silencing system based on the dual promoter method and succeeded in co-silencing URA3 and laccase in G. lucidum. The reduction of the mRNA levels of the two genes were correlated. Thus, the screening efficiency for RNAi knockdown of multiple genes may be improved by the co-silencing of an endogenous reporter gene. The molecular tools developed in this study should facilitate the isolation of genes and the characterization of the functions of multiple genes in this pharmaceutically important species, and these tools should be highly useful for the study of other basidiomycetes. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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