13 results on '"Mei, Shi"'
Search Results
2. Combining diffusion tensor imaging and gray matter volumetry to investigate motor functioning in chronic stroke.
- Author
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Ming Yang, Ya-ru Yang, Hui-jun Li, Xue-song Lu, Yong-mei Shi, Bin Liu, Hua-jun Chen, Gao-jun Teng, Rong Chen, and Edward H Herskovits
- Subjects
Medicine ,Science - Abstract
Motor impairment after stroke is related to the integrity of the corticospinal tract (CST). However, considerable variability in motor impairment remains unexplained. To increase the accuracy in evaluating long-term motor function after ischemic stroke, we tested the hypothesis that combining diffusion tensor imaging (DTI) and gray matter (GM) volumetry can better characterize long-term motor deficit than either method alone in patients with chronic stroke. We recruited 31 patients whose Medical Research Council strength grade was ≤ 3/5 in the extensor muscles of the affected upper extremity in the acute phase. We used the Upper Extremity Fugl-Meyer (UE-FM) assessment to evaluate motor impairment, and as the primary outcome variable. We computed the fractional anisotropy ratio of the entire CST (CSTratio) and the volume of interest ratio (VOIratio), between ipsilesional and contralesional hemispheres, to explain long-term motor impairment. The results showed that CSTratio, VOIratio of motor-related brain regions, and VOIratio in the temporal lobe were correlated with UE-FM. A multiple regression model including CSTratio and VOIratio of the caudate nucleus explained 40.7% of the variability in UE-FM. The adjusted R2 of the regression model with CSTratio as an independent variable was 29.4%, and that of using VOIratio of the caudate nucleus as an independent variable was 23.1%. These results suggest that combining DTI and GM volumetry may achieve better explanation of long-term motor deficit in stroke patients, than using either measure individually. This finding may provide guidance in determining optimal neurorehabilitative interventions.
- Published
- 2015
- Full Text
- View/download PDF
3. Determination of an optimal standardized uptake value of fluorodeoxyglucose for positron emission tomography imaging to assess pathological volumes of cervical cancer: a prospective study.
- Author
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Ying Zhang, Jing Hu, Hong-Jun Lu, Jian-Ping Li, Ning Wang, Wei-Wei Li, Yong-Chun Zhou, Jun-Yue Liu, Sheng-Jun Wang, Jing Wang, Xia Li, Wan-Ling Ma, Li-Chun Wei, and Mei Shi
- Subjects
Medicine ,Science - Abstract
PURPOSE: To determine the optimal standardized uptake value (SUV) of (18)F-fluorodeoxyglucose ((18)F-FDG) for positron emission tomography (PET) imaging, at which the PET-defined gross tumor volume (GTVPET) best matches with the pathological volume (GTVPATH) in the cervical cancer. MATERIALS AND METHODS: Ten patients with the cervical cancer who underwent surgery were enrolled in this study. The excised specimens were processed for whole-mount serial sections and H-E staining. The tumor borders were outlined in sections under a microscope, histopathological images were scanned and the GTVPATH calculated. The GTVPET was delineated automatically by using various percentages relative to the maximal SUV and absolute SUV. The optimal threshold SUV was further obtained as the value at which the GTVPET best matched with the GTVPATH. RESULTS: An average of 85 ± 10% shrinkage of tissue was observed after the formalin fixation. The GTVPATH was 13.38 ± 2.80 cm(3) on average. The optimal threshold on percentile SUV and absolute SUV were 40.50% ± 3.16% and 7.45 ± 1.10, respectively. The correlation analysis showed that the optimal percentile SUV threshold was inversely correlated with GTVPATH (p
- Published
- 2013
- Full Text
- View/download PDF
4. Chlorogenic acid inhibits the replication and viability of enterovirus 71 in vitro.
- Author
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Xiang Li, Yuanyuan Liu, Xueling Hou, Hongjun Peng, Li Zhang, Qingbo Jiang, Mei Shi, Yun Ji, Yuyue Wang, and Weifeng Shi
- Subjects
Medicine ,Science - Abstract
Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71. In this study, plaque reduction assay demonstrated that the inhibitory concentration 50% (IC50) of CHA on EV71 replication is 6.3 µg/ml. When both CHA (20 µg/ml) and EV71 were added, or added post-infection at different time points, CHA was able to effectively inhibit EV71 replication between 0 and 10 h. In addition, CHA inhibited EV71 2A transcription and translation in EV71-infected RD cells, but did not affect VP1, 3C, and 3D expression. Furthermore, CHA inhibited secretions of IL-6, TNF-α, IFN-γ and MCP-1 in EV71-infected RD cells. Altogether, these results revealed that CHA may have antiviral properties for treating EV71 infection.
- Published
- 2013
- Full Text
- View/download PDF
5. Insulin promotes glucose consumption via regulation of miR-99a/mTOR/PKM2 pathway.
- Author
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Wei Li, Jing Wang, Qiu-Dan Chen, Xu Qian, Qi Li, Yu Yin, Zhu-Mei Shi, Lin Wang, Jie Lin, Ling-Zhi Liu, and Bing-Hua Jiang
- Subjects
Medicine ,Science - Abstract
Insulin is known to regulate multiple cellular functions and is used for the treatment of diabetes. MicroRNAs have been demonstrated to be involved in many human diseases, including Type 2 diabetes. In this study, we showed that insulin decreased miR-99a expression levels, but induced glucose consumption and lactate production, and increased the expression of mTOR, HIF-1α and PKM2 in HepG2 and HL7702 cells. Forced expression of miR-99a or rapamycin treatment blocked insulin-induced PKM2 and HIF-1α expression, and glucose consumption and lactate production. Meanwhile, knockdown of HIF-1α inhibited PKM2 expression and insulin-induced glucose consumption. Taken together, these findings will reveal the role and mechanism of insulin in regulating glycolytic activities via miR-99a/mTOR.
- Published
- 2013
- Full Text
- View/download PDF
6. Antimicrobial peptide trichokonin VI-induced alterations in the morphological and nanomechanical properties of Bacillus subtilis.
- Author
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Hai-Nan Su, Zhi-Hua Chen, Xiao-Yan Song, Xiu-Lan Chen, Mei Shi, Bai-Cheng Zhou, Xian Zhao, and Yu-Zhong Zhang
- Subjects
Medicine ,Science - Abstract
Antimicrobial peptides are promising alternative antimicrobial agents compared to conventional antibiotics. Understanding the mode of action is important for their further application. We examined the interaction between trichokonin VI, a peptaibol isolated from Trichoderma pseudokoningii, and Bacillus subtilis, a representative Gram-positive bacterium. Trichokonin VI was effective against B. subtilis with a minimal inhibitory concentration of 25 µM. Trichokonin VI exhibited a concentration- and time-dependent effect against B. subtilis, which was studied using atomic force microscopy. The cell wall of B. subtilis collapsed and the roughness increased upon treatment with trichokonin VI. Nanoindentation experiments revealed a progressive decrease in the stiffness of the cells. Furthermore, the membrane permeabilization effect of trichokonin VI on B. subtilis was monitored, and the results suggest that the leakage of intracellular materials is a possible mechanism of action for trichokonin VI, which led to alterations in the morphological and nanomechanical properties of B. subtilis.
- Published
- 2012
- Full Text
- View/download PDF
7. MiR-128 inhibits tumor growth and angiogenesis by targeting p70S6K1.
- Author
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Zhu-mei Shi, Jing Wang, Zhiping Yan, Yong-ping You, Chong-yong Li, Xu Qian, Yu Yin, Peng Zhao, Ying-ying Wang, Xie-feng Wang, Ming-na Li, Ling-Zhi Liu, Ning Liu, and Bing-Hua Jiang
- Subjects
Medicine ,Science - Abstract
MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-128 expression levels were decreased in glioma, and identified p70S6K1 as a novel direct target of miR-128. Overexpression of miR-128 suppressed p70S6K1 and its downstream signaling molecules such as HIF-1 and VEGF expression, and attenuated cell proliferation, tumor growth and angiogenesis. Forced expression of p70S6K1 can partly rescue the inhibitory effect of miR-128 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-128 in regulating glioma tumor angiogenesis via miR-128/p70S6K1 axis, and miR-128 may serve as a potential therapeutic target in glioma in the future.
- Published
- 2012
- Full Text
- View/download PDF
8. Chlorogenic Acid Inhibits the Replication and Viability of Enterovirus 71 In Vitro
- Author
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Weifeng Shi, Xueling Hou, Yuyue Wang, Qingbo Jiang, Li Zhang, Yuanyuan Liu, Mei Shi, Yun Ji, Xiang Li, and Hongjun Peng
- Subjects
Gene Expression Regulation, Viral ,Proteases ,Time Factors ,education ,lcsh:Medicine ,Biology ,Virus Replication ,Microbiology ,Inhibitory Concentration 50 ,Interferon-gamma ,parasitic diseases ,medicine ,Enterovirus 71 ,Enterovirus Infections ,Humans ,Interferon gamma ,lcsh:Science ,Cytotoxicity ,IC50 ,Chemokine CCL2 ,Multidisciplinary ,Microbial Viability ,Molecular Structure ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,lcsh:R ,biology.organism_classification ,Molecular biology ,In vitro ,Enterovirus A, Human ,Viral replication ,lcsh:Q ,Tumor necrosis factor alpha ,Electrophoresis, Polyacrylamide Gel ,Chlorogenic Acid ,medicine.drug ,Research Article - Abstract
Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71. In this study, plaque reduction assay demonstrated that the inhibitory concentration 50% (IC50) of CHA on EV71 replication is 6.3 µg/ml. When both CHA (20 µg/ml) and EV71 were added, or added post-infection at different time points, CHA was able to effectively inhibit EV71 replication between 0 and 10 h. In addition, CHA inhibited EV71 2A transcription and translation in EV71-infected RD cells, but did not affect VP1, 3C, and 3D expression. Furthermore, CHA inhibited secretions of IL-6, TNF-α, IFN-γ and MCP-1 in EV71-infected RD cells. Altogether, these results revealed that CHA may have antiviral properties for treating EV71 infection.
- Published
- 2013
9. Insulin promotes glucose consumption via regulation of miR-99a/mTOR/PKM2 pathway
- Author
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Xu Qian, Bing-Hua Jiang, Jie Lin, Zhu Mei Shi, Lin Wang, Wei Li, Ling-Zhi Liu, Jing Wang, Qi Li, Yu Yin, and Qiu Dan Chen
- Subjects
Anatomy and Physiology ,medicine.medical_treatment ,lcsh:Medicine ,Insulin Signaling Cascade ,Molecular Cell Biology ,Insulin ,Signaling in Cellular Processes ,Phosphorylation ,RNA, Small Interfering ,Luciferases ,lcsh:Science ,Cells, Cultured ,Regulation of gene expression ,Gene knockdown ,Multidisciplinary ,Antibiotics, Antineoplastic ,Reverse Transcriptase Polymerase Chain Reaction ,TOR Serine-Threonine Kinases ,Liver Neoplasms ,Signaling Cascades ,Gene Expression Regulation, Neoplastic ,Liver ,Lactates ,medicine.drug ,Signal Transduction ,Research Article ,medicine.medical_specialty ,Thyroid Hormones ,Carcinoma, Hepatocellular ,Blotting, Western ,Endocrine System ,Carbohydrate metabolism ,PKM2 ,Biology ,Real-Time Polymerase Chain Reaction ,Glucose Signaling ,Signaling Pathways ,Downregulation and upregulation ,Internal medicine ,medicine ,Humans ,Hypoglycemic Agents ,RNA, Messenger ,PI3K/AKT/mTOR pathway ,Sirolimus ,Endocrine Physiology ,lcsh:R ,Membrane Proteins ,Hypoxia-Inducible Factor 1, alpha Subunit ,MicroRNAs ,Endocrinology ,Glucose ,lcsh:Q ,Carrier Proteins ,Tor Signaling - Abstract
Insulin is known to regulate multiple cellular functions and is used for the treatment of diabetes. MicroRNAs have been demonstrated to be involved in many human diseases, including Type 2 diabetes. In this study, we showed that insulin decreased miR-99a expression levels, but induced glucose consumption and lactate production, and increased the expression of mTOR, HIF-1α and PKM2 in HepG2 and HL7702 cells. Forced expression of miR-99a or rapamycin treatment blocked insulin-induced PKM2 and HIF-1α expression, and glucose consumption and lactate production. Meanwhile, knockdown of HIF-1α inhibited PKM2 expression and insulin-induced glucose consumption. Taken together, these findings will reveal the role and mechanism of insulin in regulating glycolytic activities via miR-99a/mTOR.
- Published
- 2013
10. Antimicrobial peptide trichokonin VI-induced alterations in the morphological and nanomechanical properties of Bacillus subtilis
- Author
-
Xian Zhao, Mei Shi, Bai-Cheng Zhou, Zhi-Hua Chen, Xiu-Lan Chen, Hai-Nan Su, Yu-Zhong Zhang, and Xiao-Yan Song
- Subjects
Proteomics ,lcsh:Medicine ,Bacillus subtilis ,Microscopy, Atomic Force ,chemistry.chemical_compound ,Anti-Infective Agents ,Microbial Physiology ,Nanotechnology ,Cell Mechanics ,Biomechanics ,lcsh:Science ,Multidisciplinary ,biology ,Chemistry ,Stem Cells ,Microbial Growth and Development ,Antimicrobial ,Bacterial Biochemistry ,Medical Microbiology ,Disease Progression ,Medicine ,medicine.symptom ,Research Article ,Surface Properties ,Ultraviolet Rays ,Antimicrobial peptides ,Peptaibol ,Biophysics ,Microbial Sensitivity Tests ,Microbiology ,Permeability ,Cell wall ,Minimum inhibitory concentration ,Complementary and Alternative Medicine ,Microbial Control ,medicine ,Synthetic Peptide ,Alamethicin ,Biology ,Models, Statistical ,lcsh:R ,Bacteriology ,biology.organism_classification ,Mechanism of action ,lcsh:Q ,Stress, Mechanical ,Peptides - Abstract
Antimicrobial peptides are promising alternative antimicrobial agents compared to conventional antibiotics. Understanding the mode of action is important for their further application. We examined the interaction between trichokonin VI, a peptaibol isolated from Trichoderma pseudokoningii, and Bacillus subtilis, a representative Gram-positive bacterium. Trichokonin VI was effective against B. subtilis with a minimal inhibitory concentration of 25 µM. Trichokonin VI exhibited a concentration- and time-dependent effect against B. subtilis, which was studied using atomic force microscopy. The cell wall of B. subtilis collapsed and the roughness increased upon treatment with trichokonin VI. Nanoindentation experiments revealed a progressive decrease in the stiffness of the cells. Furthermore, the membrane permeabilization effect of trichokonin VI on B. subtilis was monitored, and the results suggest that the leakage of intracellular materials is a possible mechanism of action for trichokonin VI, which led to alterations in the morphological and nanomechanical properties of B. subtilis.
- Published
- 2012
11. MiR-128 Inhibits Tumor Growth and Angiogenesis by Targeting p70S6K1
- Author
-
Jing Wang, Ling-Zhi Liu, Yu Yin, Zhiping Yan, Ning Liu, Bing-Hua Jiang, Chong yong Li, Xu Qian, Ying ying Wang, Ming na Li, Xie feng Wang, Peng Zhao, Yong ping You, and Zhu Mei Shi
- Subjects
Vascular Endothelial Growth Factor A ,Angiogenesis ,Cancer Treatment ,lcsh:Medicine ,Neovascularization ,Mice ,0302 clinical medicine ,Molecular Cell Biology ,lcsh:Science ,Neurological Tumors ,Regulation of gene expression ,0303 health sciences ,Mice, Inbred BALB C ,Multidisciplinary ,Neovascularization, Pathologic ,Ribosomal Protein S6 Kinases, 70-kDa ,Glioma ,Vascular endothelial growth factor A ,Oncology ,030220 oncology & carcinogenesis ,Medicine ,Epigenetics ,Hypoxia-Inducible Factor 1 ,Signal transduction ,medicine.symptom ,Cell Division ,Research Article ,Signal Transduction ,Transplantation, Heterologous ,Mice, Nude ,Biology ,03 medical and health sciences ,Cell Line, Tumor ,microRNA ,medicine ,Genetics ,Animals ,Humans ,030304 developmental biology ,Cell growth ,lcsh:R ,Cancers and Neoplasms ,medicine.disease ,Molecular biology ,MicroRNAs ,Cancer research ,lcsh:Q ,Neoplasm Transplantation - Abstract
MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-128 expression levels were decreased in glioma, and identified p70S6K1 as a novel direct target of miR-128. Overexpression of miR-128 suppressed p70S6K1 and its downstream signaling molecules such as HIF-1 and VEGF expression, and attenuated cell proliferation, tumor growth and angiogenesis. Forced expression of p70S6K1 can partly rescue the inhibitory effect of miR-128 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-128 in regulating glioma tumor angiogenesis via miR-128/p70S6K1 axis, and miR-128 may serve as a potential therapeutic target in glioma in the future.
- Published
- 2012
12. Resveratrol Inhibits Enterovirus 71 Replication and Pro-Inflammatory Cytokine Secretion in Rhabdosarcoma Cells through Blocking IKKs/NF-κB Signaling Pathway
- Author
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Jingtin Jiang, Jing Sun, Weifeng Shi, Zhiwen Gu, Xiaopeng Xu, Mei Shi, Yuyue Wang, Li Zhang, Lirong Zhang, Yun Ji, and Yuanyuan Li
- Subjects
endocrine system diseases ,lcsh:Medicine ,Resveratrol ,Biology ,Pharmacology ,Virus Replication ,Antiviral Agents ,chemistry.chemical_compound ,Glucosides ,Rhabdomyosarcoma ,Stilbenes ,Secretion ,Phosphorylation ,lcsh:Science ,skin and connective tissue diseases ,Interleukin 6 ,Inflammation ,Multidisciplinary ,Dose-Response Relationship, Drug ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,organic chemicals ,lcsh:R ,NF-kappa B ,food and beverages ,NFKB1 ,Enterovirus A, Human ,I-kappa B Kinase ,chemistry ,Biochemistry ,biology.protein ,Cytokines ,lcsh:Q ,Cytokine secretion ,Tumor necrosis factor alpha ,Signal transduction ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction ,Research Article - Abstract
Polydatin and resveratrol, as major active components in Polygonum cuspidatum, have anti-inflammatory, antioxidant and antitumor functions. However, the effect and mechanism of polydatin and resveratrol on enterovirus 71 (EV71) have not been reported. In this study, resveratrol revealed strong antiviral activity on EV71, while polydatin had weak effect. Neither polydatin nor resveratrol exhibited influence on viral attachment. Resveratrol could effectively inhibit the synthesis of EV71/VP1 and the phosphorylation of IKKα, IKKβ, IKKγ, IKBα, NF-κB p50 and NF-κB p65, respectively. Meanwhile, the remarkably increased secretion of IL-6 and TNF-α in EV71-infected rhabdosarcoma (RD) cells could be blocked by resveratrol. These results demonstrated that resveratrol inhibited EV71 replication and cytokine secretion in EV71-infected RD cells through blocking IKKs/NF-κB signaling pathway. Thus, resveratrol may have potent antiviral effect on EV71 infection.
- Published
- 2015
- Full Text
- View/download PDF
13. Determination of an Optimal Standardized Uptake Value of Fluorodeoxyglucose for Positron Emission Tomography Imaging to Assess Pathological Volumes of Cervical Cancer: A Prospective Study
- Author
-
Ning Wang, Sheng-jun Wang, Jing Hu, Hong-Jun Lu, Xia Li, Jian-Ping Li, Jun-yue Liu, Yong-Chun Zhou, Wan-Ling Ma, Jing Wang, Wei-Wei Li, Mei Shi, Lichun Wei, and Ying Zhang
- Subjects
Adult ,Percentile ,medicine.medical_specialty ,medicine.medical_treatment ,Uterine Cervical Neoplasms ,lcsh:Medicine ,Standardized uptake value ,Fluorodeoxyglucose F18 ,Reference Values ,medicine ,Humans ,Prospective Studies ,lcsh:Science ,Prospective cohort study ,Neoplasm Staging ,Fluorodeoxyglucose ,Cervical cancer ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Tumor Burden ,Radiation therapy ,Positron emission tomography ,Positron-Emission Tomography ,Female ,lcsh:Q ,Radiology ,Tomography, X-Ray Computed ,business ,Nuclear medicine ,Research Article ,medicine.drug - Abstract
PURPOSE: To determine the optimal standardized uptake value (SUV) of (18)F-fluorodeoxyglucose ((18)F-FDG) for positron emission tomography (PET) imaging, at which the PET-defined gross tumor volume (GTVPET) best matches with the pathological volume (GTVPATH) in the cervical cancer. MATERIALS AND METHODS: Ten patients with the cervical cancer who underwent surgery were enrolled in this study. The excised specimens were processed for whole-mount serial sections and H-E staining. The tumor borders were outlined in sections under a microscope, histopathological images were scanned and the GTVPATH calculated. The GTVPET was delineated automatically by using various percentages relative to the maximal SUV and absolute SUV. The optimal threshold SUV was further obtained as the value at which the GTVPET best matched with the GTVPATH. RESULTS: An average of 85 ± 10% shrinkage of tissue was observed after the formalin fixation. The GTVPATH was 13.38 ± 2.80 cm(3) on average. The optimal threshold on percentile SUV and absolute SUV were 40.50% ± 3.16% and 7.45 ± 1.10, respectively. The correlation analysis showed that the optimal percentile SUV threshold was inversely correlated with GTVPATH (p
- Published
- 2013
- Full Text
- View/download PDF
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