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Your search keyword '"Messenger/genetics"' showing total 2 results
Start Over Descriptor "Messenger/genetics" Journal plos one
2 results on '"Messenger/genetics"'

1. Wnt5a Deficiency Leads to Anomalies in Ureteric Tree Development, Tubular Epithelial Cell Organization and Basement Membrane Integrity Pointing to a Role in Kidney Collecting Duct Patterning

Author

Susanna Kaisto, Antti M. Salo, Kirsten Y. Renkema, Leonardo D. Garma, Wout F.J. Feitz, Ernie M.H.F. Bongers, Ilkka Miinalainen, Elisavet Tika, Johanna Myllyharju, André H. Juffer, Ilkka Pietilä, Nine V A M Knoers, Albertien M. van Eerde, Seppo Vainio, and Renata Prunskaite-Hyyryläinen

Subjects
Pathology, Organogenesis, Developmental Signaling, Biochemistry, Immunoenzyme Techniques, Mice, Cell Signaling, Laminin, Biochemical Simulations, Child, lcsh:Science, Cells, Cultured, In Situ Hybridization, Cultured, Hyperplasia, Kidney Tubules, Cellular Structures and Organelles, Kidney Tubules, Collecting/metabolism, Rare cancers Radboud Institute for Health Sciences [Radboudumc 9], medicine.medical_specialty, Cells, Knockout, Wnt-5a Protein, 03 medical and health sciences, Genetics, Humans, RNA, Messenger, Kidney Tubules, Collecting, Vesico-Ureteral Reflux, Embryos, lcsh:R, Biology and Life Sciences, Proteins, Computational Biology, Epithelial Cells, medicine.disease, Wnt Proteins, body regions, 030104 developmental biology, Urogenital Abnormalities, Mutation, Messenger/genetics, lcsh:Q, Ureter, Organism Development, Developmental Biology, 0301 basic medicine, RNA, Messenger/genetics, Embryology, Protein Conformation, Gene Expression, lcsh:Medicine, Basement Membrane, Type IV collagen, Wnt4 Protein, Ureter/embryology, Medicine and Health Sciences, Morphogenesis, Non-U.S. Gov't, Mice, Knockout, Kidney, Multidisciplinary, Reverse Transcriptase Polymerase Chain Reaction, Research Support, Non-U.S. Gov't, Vesico-Ureteral Reflux/physiopathology, Hypoplasia, Extracellular Matrix, medicine.anatomical_structure, Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10], Embryo, Mammalian/cytology, Embryo, Ureteric bud, embryonic structures, Urogenital Abnormalities/physiopathology, Female, Anatomy, Research Article, Signal Transduction, Adolescent, Mutation/genetics, Biology, Research Support, Real-Time Polymerase Chain Reaction, Epithelial Cells/cytology, Wnt4 Protein/physiology, medicine, Journal Article, Animals, Mammalian/cytology, Basement membrane, Basement Membrane/metabolism, Wnt Proteins/chemistry, Kidneys, Renal System, Cell Biology, Embryo, Mammalian, Molecular biology, Epithelium, biology.protein, RNA, Collecting/metabolism, sense organs, Collagens
Abstract

Contains fulltext : 171962.PDF (Publisher’s version ) (Open Access) The Wnts can be considered as candidates for the Congenital Anomaly of Kidney and Urinary Tract, CAKUT diseases since they take part in the control of kidney organogenesis. Of them Wnt5a is expressed in ureteric bud (UB) and its deficiency leads to duplex collecting system (13/90) uni- or bilateral kidney agenesis (10/90), hypoplasia with altered pattern of ureteric tree organization (42/90) and lobularization defects with partly fused ureter trunks (25/90) unlike in controls. The UB had also notably less tips due to Wnt5a deficiency being at E15.5 306 and at E16.5 765 corresponding to 428 and 1022 in control (p

Published
2016

2. A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence

Author

Susanne M. Kooistra, Michael Lees, Cornelia Steinhauer, Jens Vilstrup Johansen, Lise Christine Rudkjær Nørgaard, and Kristian Helin

Subjects
RNA, Messenger/genetics, Small interfering RNA, Gene Expression, medicine.disease_cause, Biochemistry, RNA interference, Nucleic Acids, Molecular Cell Biology, Basic Cancer Research, Medicine and Health Sciences, Cellular Senescence, Cellular Stress Responses, Regulation of gene expression, Multidisciplinary, Cancer Risk Factors, Cell biology, Gene Expression Regulation, Neoplastic, Cyclin-Dependent Kinase Inhibitor p16/metabolism, Cellular Senescence/genetics, Oncology, Cell Processes, Medicine, Epigenetics, Cell aging, Research Article, Senescence, Science, Genetic Causes of Cancer, Biology, Cell Line, microRNA, medicine, Genetics, Cancer Genetics, Humans, RNA, Messenger, Cyclin-Dependent Kinase Inhibitor p16, Neoplastic, Oncogene, Biology and life sciences, Oncogenes, Cell Biology, MicroRNAs, MicroRNAs/genetics, Gene Expression Regulation, Messenger/genetics, RNA, Ectopic expression, Carcinogenesis
Abstract

Oncogene-induced senescence (OIS) can occur in response to hyperactive oncogenic signals and is believed to be a fail-safe mechanism protecting against tumorigenesis. To identify new factors involved in OIS, we performed a screen for microRNAs that can overcome or inhibit OIS in human diploid fibroblasts. This screen led to the identification of miR-378a-5p and in addition several other miRNAs that have previously been shown to play a role in senescence. We show that ectopic expression of miR-378a-5p reduces the expression of several senescence markers, including p16(INK4A) and senescence-associated β-galactosidase. Moreover, cells with ectopic expression of miR-378a-5p retain proliferative capacity even in the presence of an activated Braf oncogene. Finally, we identified several miR-378a-5p targets in diploid fibroblasts that might explain the mechanism by which the microRNA can delay OIS. We speculate that miR-378a-5p might positively influence tumor formation by delaying OIS, which is consistent with a known pro-oncogenic function of this microRNA.

Published
2014

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