1. Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera.
- Author
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Miao, Yu-Tong, Deng, Yao, Jia, Hao-Kang, Liu, Yu-Di, and Hou, Mao-Lin
- Subjects
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SALIVA analysis , *PLANTHOPPERS , *DNA , *SALIVARY glands , *BODY fluids - Abstract
The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins. Gene expression pattern of 11 secretory proteins were analyzed by real time quantitative-PCR. We detected the mucin-like protein, which had a unique expression level in salivary gland, most likely to be a candidate effector involved in regulation of plant defense. This study identified the watery saliva component of S. furcifera and it provided a list of proteins which may play a role in interaction between S. furcifera and rice. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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