Search

Your search keyword '"Mie Kobayashi-Ishihara"' showing total 2 results
Start Over Author "Mie Kobayashi-Ishihara" Journal plos one
2 results on '"Mie Kobayashi-Ishihara"'

1. Broad cross-reactive epitopes of the H5N1 influenza virus identified by murine antibodies against the A/Vietnam/1194/2004 hemagglutinin.

Author

Mie Kobayashi-Ishihara, Hitoshi Takahashi, Kazuo Ohnishi, Kengo Nishimura, Kazutaka Terahara, Manabu Ato, Shigeyuki Itamura, Tsutomu Kageyama, and Yasuko Tsunetsugu-Yokota

Subjects
Medicine, Science
Abstract

There is an urgent need for a rapid diagnostic system to detect the H5 subtype of the influenza A virus. We previously developed monoclonal antibodies (mAbs) against the H5 hemagglutinin (HA) for use in a rapid diagnostic kit. In this study, we determined the epitopes of the anti-H5 HA murine mAbs OM-b, AY-2C2, and YH-1A1. Binding assays of the mAbs to different strains of H5 HAs indicated that OM-b and AY-2C2 cross-reacted with HAs from clades 1, 2.1.3.2, 2.2, and 2.3.4, whereas YH-1A1 failed to bind to those of clades 2.1.3.2 and 2.3.4. HA chimeras revealed that the epitopes for each of the mAbs were in the HA1 region. Analysis of escape mutants revealed that OM-b and AY-2C2 mAbs interacted mainly with amino acid residues D43 and G46, and the YH-1A1 mAb interacted with G139 and K or R140 of H5 HA. Multiple alignments of H5 HA protein sequences showed that D43 and G46 were very conserved among H5N1 HAs, except those in clade 2.2.1 and clade 7 (88.7%). The epitope for YH-1A1 mAb was highly variable in the HAs of H5N1, although it was well conserved in those of H5N2-N9. The OM-b and AY-2C2 mAbs could bind to the HAs of clades 1.1 and 2.3.2.1 that are currently epidemic in Asia, and we conclude that these would be effective for the detection of H5N1 infections in this region.

Published
2014
Full Text
View/download PDF

2. Broad Cross-Reactive Epitopes of the H5N1 Influenza Virus Identified by Murine Antibodies against the A/Vietnam/1194/2004 Hemagglutinin

Author

Tsutomu Kageyama, Kazuo Ohnishi, Hitoshi Takahashi, Yasuko Tsunetsugu-Yokota, Mie Kobayashi-Ishihara, Kengo Nishimura, Shigeyuki Itamura, Kazutaka Terahara, and Manabu Ato

Subjects
Viral Diseases, medicine.drug_class, Immunology, Hemagglutinin (influenza), lcsh:Medicine, Enzyme-Linked Immunosorbent Assay, Hemagglutinin Glycoproteins, Influenza Virus, Cross Reactions, Monoclonal antibody, medicine.disease_cause, Microbiology, Epitope, Virus, Epitopes, Mice, Diagnostic Medicine, Virology, Emerging Viral Diseases, Influenza, Human, medicine, Influenza A virus, Medicine and Health Sciences, Animals, Humans, lcsh:Science, Multidisciplinary, biology, Influenza A Virus, H5N1 Subtype, lcsh:R, Biology and Life Sciences, Antibodies, Monoclonal, Influenza A virus subtype H5N1, Clinical Laboratory Sciences, Influenza, Viral Disease Diagnosis, Epitope mapping, Infectious Diseases, biology.protein, lcsh:Q, Clinical Immunology, Antibody, Epitope Mapping, Research Article
Abstract

There is an urgent need for a rapid diagnostic system to detect the H5 subtype of the influenza A virus. We previously developed monoclonal antibodies (mAbs) against the H5 hemagglutinin (HA) for use in a rapid diagnostic kit. In this study, we determined the epitopes of the anti-H5 HA murine mAbs OM-b, AY-2C2, and YH-1A1. Binding assays of the mAbs to different strains of H5 HAs indicated that OM-b and AY-2C2 cross-reacted with HAs from clades 1, 2.1.3.2, 2.2, and 2.3.4, whereas YH-1A1 failed to bind to those of clades 2.1.3.2 and 2.3.4. HA chimeras revealed that the epitopes for each of the mAbs were in the HA1 region. Analysis of escape mutants revealed that OM-b and AY-2C2 mAbs interacted mainly with amino acid residues D43 and G46, and the YH-1A1 mAb interacted with G139 and K or R140 of H5 HA. Multiple alignments of H5 HA protein sequences showed that D43 and G46 were very conserved among H5N1 HAs, except those in clade 2.2.1 and clade 7 (88.7%). The epitope for YH-1A1 mAb was highly variable in the HAs of H5N1, although it was well conserved in those of H5N2-N9. The OM-b and AY-2C2 mAbs could bind to the HAs of clades 1.1 and 2.3.2.1 that are currently epidemic in Asia, and we conclude that these would be effective for the detection of H5N1 infections in this region.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results