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1. Diagnostic accuracy and acceptability of molecular diagnosis of COVID-19 on saliva samples relative to nasopharyngeal swabs in tropical hospital and extra-hospital contexts: The COVISAL study.

Author

Mathieu Nacher, Mayka Mergeay-Fabre, Denis Blanchet, Orelie Benois, Tristan Pozl, Pauline Mesphoule, Vincent Sainte-Rose, Véronique Vialette, Bruno Toulet, Aurélie Moua, Mona Saout, Stéphane Simon, Manon Guidarelli, Muriel Galindo, Barbara Biche, William Faurous, Laurie Chaizemartin, Aniza Fahrasmane, Devi Rochemont, Fode Diop, Moussa Niang, Jean Pujo, Nicolas Vignier, Dominique Dotou, Astrid Vabret, and Magalie Demar

Subjects
Medicine, Science
Abstract

A prospective study was conducted among different intra and extra-hospital populations of French Guiana to evaluate the performance of saliva testing compared to nasopharyngeal swabs. Persons aged 3 years and older with mild symptoms suggestive of COVID-19 and asymptomatic persons with a testing indication were prospectively enrolled. Nasopharyngeal and salivary samples were stored at 4°C before analysis. Both samples were analyzed with the same Real-time PCR amplification of E gene, N gene, and RdRp gene. Between July 22th and October 28th, 1159 persons were included, of which 1028 were analyzed. When only considering as positives those with 2 target genes with Ct values

Published
2021
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2. Diagnostic accuracy and acceptability of molecular diagnosis of COVID-19 on saliva samples relative to nasopharyngeal swabs in tropical hospital and extra-hospital contexts: The COVISAL study

Author

Stéphane Simon, Mathieu Nacher, Devi Rochemont, Laurie Chaizemartin, Aurélie Moua, Muriel Galindo, Magalie Demar, Nicolas Vignier, Astrid Vabret, Bruno Toulet, Mayka Mergeay-Fabre, Manon Guidarelli, Pauline Mesphoule, Dominique Dotou, Denis Blanchet, Orelie Benois, Vincent Sainte-Rose, Fode Diop, William Faurous, Mona Saout, Moussa Niang, Barbara Biche, Aniza Fahrasmane, Véronique Vialette, Tristan Pozl, and Jean Marc Pujo

Subjects
Male, Saliva, Viral Diseases, Physiology, Diagnostic accuracy, Artificial Gene Amplification and Extension, Polymerase Chain Reaction, Medical Conditions, Positive predicative value, Nasopharynx, Medicine and Health Sciences, Medicine, Prospective cohort study, Child, Virus Testing, Multidisciplinary, Middle Aged, Hospitals, Body Fluids, French Guiana, Infectious Diseases, COVID-19 Nucleic Acid Testing, Child, Preschool, Female, medicine.symptom, Anatomy, Research Article, Adult, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Adolescent, Science, Research and Analysis Methods, Asymptomatic, Microbiology, Sensitivity and Specificity, Saliva testing, Diagnostic Medicine, Internal medicine, Virology, Viral Nucleic Acid, Humans, Molecular Biology Techniques, Molecular Biology, Aged, Tropical Climate, business.industry, Biology and Life Sciences, Covid 19, Reverse Transcriptase-Polymerase Chain Reaction, Patient Acceptance of Health Care, Viral Replication, Mild symptoms, Age Groups, People and Places, Population Groupings, business
Abstract

A prospective study was conducted among different intra and extra-hospital populations of French Guiana to evaluate the performance of saliva testing compared to nasopharyngeal swabs. Persons aged 3 years and older with mild symptoms suggestive of COVID-19 and asymptomatic persons with a testing indication were prospectively enrolled. Nasopharyngeal and salivary samples were stored at 4°C before analysis. Both samples were analyzed with the same Real-time PCR amplification of E gene, N gene, and RdRp gene. Between July 22th and October 28th, 1159 persons were included, of which 1028 were analyzed. When only considering as positives those with 2 target genes with Ct values

Published
2021

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