1. A human genotyping trial to estimate the post-feeding time from mosquito blood meals
- Author
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Toshimichi Yamamoto, Masaaki Hara, Nakayama Koji, Hikitsuchi Tomoyuki, Kawajiri Yumi, Aya Takada, Mitsuo Umeda, Atsushi Nagai, Yuuji Hiroshige, Akira Ishii, and Koichi Suzuki
- Subjects
0301 basic medicine ,Male ,Veterinary medicine ,Genotyping Techniques ,Physiology ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Disease Vectors ,Mosquitoes ,Polymerase Chain Reaction ,law.invention ,0302 clinical medicine ,law ,Aedes ,Abdomen ,Medicine and Health Sciences ,lcsh:Science ,DNA extraction ,Polymerase chain reaction ,Genetics ,Multidisciplinary ,biology ,Genomics ,Body Fluids ,Insects ,Culex ,Tandem Repeats ,Blood ,Infectious Diseases ,Microsatellite ,Animal Nutritional Physiological Phenomena ,Female ,Anatomy ,Research Article ,Genotyping ,Aedes albopictus ,Arthropoda ,030231 tropical medicine ,Research and Analysis Methods ,03 medical and health sciences ,Extraction techniques ,Animals ,Humans ,Repeated Sequences ,Molecular Biology Techniques ,Molecular Biology ,lcsh:R ,Organisms ,Biology and Life Sciences ,DNA ,biology.organism_classification ,Blood meal ,Invertebrates ,Insect Vectors ,Species Interactions ,030104 developmental biology ,Genetic Loci ,lcsh:Q ,Reagent Kits, Diagnostic - Abstract
Mosquitoes occur almost worldwide, and females of some species feed on blood from humans and other animals to support ovum maturation. In warm and hot seasons, such as the summer in Japan, fed mosquitoes are often observed at crime scenes. The current study attempted to estimate the time that elapsed since feeding from the degree of human DNA digestion in mosquito blood meals and also to identify the individual human sources of the DNA using genotyping in two species of mosquito: Culex pipiens pallens and Aedes albopictus. After stereomicroscopic observation, the extracted DNA samples were quantified using a human DNA quantification and quality control kit and were genotyped for 15 short tandem repeats using a commercial multiplexing kit. It took about 3 days for the complete digestion of a blood meal, and genotyping was possible until 2 days post-feeding. The relative peak heights of the 15 STRs and DNA concentrations were useful for estimating the post-feeding time to approximately half a day between 0 and 2 days. Furthermore, the quantitative ratios derived from STR peak heights and the quality control kit (Q129/Q41, Q305/Q41, and Q305/Q129) were reasonably effective for estimating the approximate post-feeding time after 2-3 days. We suggest that this study may be very useful for estimating the time since a mosquito fed from blood meal DNA, although further refinements are necessary to estimate the times more accurately.
- Published
- 2017