Search

Your search keyword '"Ryan M Stepan"' showing total 2 results
Start Over Author "Ryan M Stepan" Journal plos one
2 results on '"Ryan M Stepan"'

1. Characterization of Staphylococcus aureus from Humans and a Comparison with İsolates of Animal Origin, in North Dakota, United States.

Author

Valeria Velasco, Esra Buyukcangaz, Julie S Sherwood, Ryan M Stepan, Ryan J Koslofsky, and Catherine M Logue

Subjects
Medicine, Science
Abstract

Different clones of methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus have been found in humans as well as in animals and retail meat. However, more information about the genetic characteristics and similarities between strains is needed. The aim of this study was to identify and characterize Staphylococcus aureus from humans, and to compare their characteristics with isolates of animal origin. A total of 550 nasal swabs were taken from healthy humans, and S. aureus was isolated and identified. Positive S. aureus isolates were subjected to molecular typing and susceptibility testing. In addition, 108 MRSA isolates recovered from clinical patients in the state of North Dakota and 133 S. aureus isolates from animals and meat previously analyzed were included. The nasal carriage of S. aureus in healthy people was 7.6% and, in general, clones were genetically diverse. None of the S. aureus strains obtained from healthy people were mecA- or PVL-positive. A total of 105 (97.2%) MRSA isolates from clinical cases harbored the mecA gene and 11 (10.2%) isolated from blood stream infections harbored the PVL gene. The most common resistance profile among S. aureus from healthy people was penicillin, and from clinical cases were erythromycin-penicillin-ciprofloxacin. The rate of multidrug resistance (MDR) was 70% in humans. Most of the S. aureus harboring mecA and PVL genes were identified as ST5 and ST8, and exhibited MDR. However, S. aureus isolates of animal origin used for comparison exhibited a lower rate of MDR. The most common resistance profiles in isolates of animal origin were penicillin-tetracycline and penicillin-tetracycline-erythromycin, in animals and raw meat, respectively. The ST5 was also found in animals and meat, with ST9 and ST398 being the major clones. The genetic similarity between clones from humans and meat suggests the risk of spread of S. aureus in the food chain.

Published
2015
Full Text
View/download PDF

2. Characterization of Staphylococcus aureus from Humans and a Comparison with İsolates of Animal Origin, in North Dakota, United States

Author

Catherine M. Logue, Ryan M Stepan, Julie S. Sherwood, Esra Büyükcangaz, Ryan J. Koslofsky, Valeria Velasco, Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı., Büyükcangaz, Esra, and AAL-2323-2020

Subjects
Bacterium identification, Identification, Science & technology - other topics, Antibiotic resistance, lcsh:Medicine, Strains, Bacterial strain, Molecular typing, Multidrug resistance, medicine.disease_cause, Procedures, MecA gene, Bacterial protein, Staphylococcus infection, Ciprofloxacin, Bacterial transmission, Drug Resistance, Multiple, Bacterial, RNA, Ribosomal, 16S, Prevalence, Cluster Analysis, lcsh:Science, Genetic similarity, Phylogeny, Multidisciplinary, Bacterial gene, Mrsa, Bovine, Microbial sensitivity test, Staphylococcal Infections, Methicillin-resistant, Classification, Nose cavity, Chicken, Erythromycin, Normal human, Staphylococcus aureus, North Dakota, Penicillin derivative, Animals, Meticillin, Cefoxitin, Food control, Nasal Cavity, Human, Research Article, Methicillin-Resistant Staphylococcus aureus, Bacterium isolate, Meat, RNA 16S, Clone, Microbial Sensitivity Tests, Biology, Staphylococcal infections, Infections, Microbiology, Multidisciplinary sciences, Article, Young Adult, Bacterial Proteins, medicine, Genetics, Humans, Raw meat, Sheep, Animal, SCCmec, lcsh:R, Tetracycline, biochemical phenomena, metabolism, and nutrition, medicine.disease, Nonhuman, bacterial infections and mycoses, Methicillin-resistant Staphylococcus aureus, United States, Multiple drug resistance, Drug effect, PVL gene, Isolation and purification, Food Microbiology, Multilocus sequence typing, Strain difference, lcsh:Q, Genetic variability, Cattle, Comparative study, Controlled study, Chickens, Methicillin resistant Staphylococcus aureus, Multilocus Sequence Typing
Abstract

Different clones of methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus have been found in humans as well as in animals and retail meat. However, more information about the genetic characteristics and similarities between strains is needed. The aim of this study was to identify and characterize Staphylococcus aureus from humans, and to compare their characteristics with isolates of animal origin. A total of 550 nasal swabs were taken from healthy humans, and S. aureus was isolated and identified. Positive S. aureus isolates were subjected to molecular typing and susceptibility testing. In addition, 108 MRSA isolates recovered from clinical patients in the state of North Dakota and 133 S. aureus isolates from animals and meat previously analyzed were included. The nasal carriage of S. aureus in healthy people was 7.6% and, in general, clones were genetically diverse. None of the S. aureus strains obtained from healthy people were mecA-or PVL-positive. A total of 105 (97.2%) MRSA isolates from clinical cases harbored the mecA gene and 11 (10.2%) isolated from blood stream infections harbored the PVL gene. The most common resistance profile among S. aureus from healthy people was penicillin, and from clinical cases were erythromycin-penicillin-ciprofloxacin. The rate of multidrug resistance (MDR) was 70% in humans. Most of the S. aureus harboring mecA and PVL genes were identified as ST5 and ST8, and exhibited MDR. However, S. aureus isolates of animal origin used for comparison exhibited a lower rate of MDR. The most common resistance profiles in isolates of animal origin were penicillin-tetracycline and penicillin-tetracycline-erythromycin, in animals and raw meat, respectively. The ST5 was also found in animals and meat, with ST9 and ST398 being the major clones. The genetic similarity between clones from humans and meat suggests the risk of spread of S. aureus in the food chain. Dean's Office, College of Veterinary Medicine, Iowa State University, Ames, Iowa Dean's Office, College of Agriculture, Food Systems and Natural Resources College, North Dakota State University

Published
2015

Catalog

Books, media, physical & digital resources
See catalog results