1. A novel chloroplast super-complex consisting of the ATP synthase and photosystem I reaction center
- Author
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Bhaduri, Satarupa, Singh, Sandeep K, Cohn, Whitaker, Hasan, S Saif, Whitelegge, Julian P, and Cramer, William A
- Subjects
Chemical Sciences ,Physical Chemistry ,Affordable and Clean Energy ,Adenosine Triphosphate ,Chloroplasts ,Electron Transport ,Ferredoxin-NADP Reductase ,Nitric Oxide Synthase ,Photosynthesis ,Photosystem I Protein Complex ,Photosystem II Protein Complex ,Plant Leaves ,Plant Proteins ,Proton-Translocating ATPases ,Spinacia oleracea ,Thylakoids ,General Science & Technology - Abstract
Several 'super-complexes' of individual hetero-oligomeric membrane protein complexes, whose function is to facilitate intra-membrane electron and proton transfer and harvesting of light energy, have been previously characterized in the mitochondrial cristae and chloroplast thylakoid membranes. We report the presence of an intra-membrane super-complex dominated by the ATP-synthase, photosystem I (PSI) reaction-center complex and the ferredoxin-NADP+ Reductase (FNR) in the thylakoid membrane. The presence of the super-complex has been documented by mass spectrometry, clear-native PAGE and Western Blot analyses. This is the first documented presence of ATP synthase in a super-complex with the PSI reaction-center located in the non-appressed stromal domain of the thylakoid membrane.
- Published
- 2020