8 results on '"Tamar Peretz"'
Search Results
2. Checkpoint inhibitors: Better outcomes among advanced cutaneous head and neck melanoma patients.
- Author
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Nir Hirshoren, Roni Yoeli, Jonathan E Cohen, Jeffrey M Weinberger, Nadia Kaplan, Sharon Merims, Tamar Peretz, and Michal Lotem
- Subjects
Medicine ,Science - Abstract
OBJECTIVE:The aim of this study was to investigate if the treatment outcomes of checkpoint inhibitors (CPI) in patients with advanced-stage skin head and neck melanoma (HNM) differs from outcomes in patients with non-HNM. DESIGN:A retrospective cohort study of patients with unresectable AJCC stage III and stage IV, who received CPI between 2010 and 2017. PARTICIPANTS:Overall, 122 unresectable AJCC stage III and metastatic stage IV melanoma adult patients were treated with CPI during the study period (consecutive patients). The HNM group of patients was comparable with limbs and trunk melanoma group except different distant metastatic (M1a/b/c/d) pattern (p = 0.025). MAIN OUTCOMES:Comparison of overall survival and clinical response to CPI in patients with advanced-stage skin melanoma of the head and neck with non-HNM. RESULTS:We analyzed 38 patients with melanoma arising in the head and neck skin regions, 33 with melanoma of limbs and 51 with trunk melanoma. Most of the head and neck patients were men (89.5%), the average age of melanoma diagnosis was 61.4±16.7 years (range 16.4-85.6). More than a third of HNM group of patients (36.8%) were 70 years and older. Overall response rate (ORR) to CPI was 50% (CR 31.6% and PR 18.4%) in the head and neck study group of patients, compared to an ORR of 36.3% and 23.5% in melanoma of the limbs and of the trunk, respectively (p = 0.03). The median overall survival of HNM group of patients was 60.2±6.3 months, CI 95% [47.7-72.7], 63% were alive at 30 months, reaching a plateau. Whereas, the median survival time of limbs and trunk melanoma were 51.2 and 53.4 months, which did not reach significance. CONCLUSIONS AND RELEVANCE:Response rate to CPI is significantly improved in patients with melanoma of the head and neck and they have a trend towards improved, long standing, overall survival.
- Published
- 2020
- Full Text
- View/download PDF
3. Feasibility of Unbiased RNA Profiling of Colorectal Tumors: A Proof of Principle.
- Author
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Vardit Moshayoff, Ouriel Faktor, Luigi Laghi, Giuseppe Celesti, Tamar Peretz, Dan Keret, Dana Cohen, and Eran Israeli
- Subjects
Medicine ,Science - Abstract
Despite recent advances in molecular profiling of colorectal cancer (CRC), as of yet this has not translated into an unbiased molecular liquid biopsy profile which can accurately screen for early CRC. In this study we depict the profile of early stage CRC as well as for advanced adenomas (AA) by combination of current molecular knowledge with microarray technology, using efficient circulating free plasma RNA purification from blood and RNA amplification technologies. We joined literature search with Affymetrix gene chip experimental procedure to draw the circulating free plasma RNA profile of colorectal cancer disease reflected in blood. The RNA panel was tested by two datasets comparing patients with CRC with healthy subjects and patients with AA to healthy subjects. For the CRC patient cohort (28 CRC cases vs. 41 healthy controls), the ROC analysis of the selected biomarker panel generated a sensitivity of 75% and a specificity of 93% for the detection of CRC using 8-gene classification model. For the AA patient cohort (28 subjects vs. 46 healthy controls), a sensitivity of 60% and a specificity of 87% were calculated using a 2-gene classification model. We have identified a panel of 8 plasma RNA markers as a preliminary panel for CRC detection and subset markers suitable for AA detection. Subjected to extensive clinical validation we suggest that this panel represents a feasible approach and a potential strategy for noninvasive early diagnosis, as a first-line screening test for asymptomatic, average-risk population before colonoscopy.
- Published
- 2016
- Full Text
- View/download PDF
4. Human T cell crosstalk is induced by tumor membrane transfer.
- Author
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Ronny Uzana, Galit Eisenberg, Sharon Merims, Shoshana Frankenburg, Aviad Pato, Eitan Yefenof, Roni Engelstein, Tamar Peretz, Arthur Machlenkin, and Michal Lotem
- Subjects
Medicine ,Science - Abstract
Trogocytosis is a contact-dependent unidirectional transfer of membrane fragments between immune effector cells and their targets, initially detected in T cells following interaction with professional antigen presenting cells (APC). Previously, we have demonstrated that trogocytosis also takes place between melanoma-specific cytotoxic T lymphocytes (CTLs) and their cognate tumors. In the present study, we took this finding a step further, focusing on the ability of melanoma membrane-imprinted CD8+ T cells to act as APCs (CD8+ T-APCs). We demonstrate that, following trogocytosis, CD8+ T-APCs directly present a variety of melanoma derived peptides to fraternal T cells with the same TCR specificity or to T cells with different TCRs. The resulting T cell-T cell immune synapse leads to (1) Activation of effector CTLs, as determined by proliferation, cytokine secretion and degranulation; (2) Fratricide (killing) of CD8+ T-APCs by the activated CTLs. Thus, trogocytosis enables cross-reactivity among CD8+ T cells with interchanging roles of effectors and APCs. This dual function of tumor-reactive CTLs may hint at their ability to amplify or restrict reactivity against the tumor and participate in modulation of the anti-cancer immune response.
- Published
- 2015
- Full Text
- View/download PDF
5. Newly generated heparanase knock-out mice unravel co-regulation of heparanase and matrix metalloproteinases.
- Author
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Eyal Zcharia, Juan Jia, Xiao Zhang, Lea Baraz, Ulf Lindahl, Tamar Peretz, Israel Vlodavsky, and Jin-Ping Li
- Subjects
Medicine ,Science - Abstract
Heparanase, a mammalian endo-beta-D-glucuronidase, specifically degrades heparan sulfate proteoglycans ubiquitously associated with the cell surface and extracellular matrix. This single gene encoded enzyme is over-expressed in most human cancers, promoting tumor metastasis and angiogenesis.We report that targeted disruption of the murine heparanase gene eliminated heparanase enzymatic activity, resulting in accumulation of long heparan sulfate chains. Unexpectedly, the heparanase knockout (Hpse-KO) mice were fertile, exhibited a normal life span and did not show prominent pathological alterations. The lack of major abnormalities is attributed to a marked elevation in the expression of matrix metalloproteinases, for example, MMP2 and MMP14 in the Hpse-KO liver and kidney. Co-regulation of heparanase and MMPs was also noted by a marked decrease in MMP (primarily MMP-2,-9 and 14) expression following transfection and over-expression of the heparanase gene in cultured human mammary carcinoma (MDA-MB-231) cells. Immunostaining (kidney tissue) and chromatin immunoprecipitation (ChIP) analysis (Hpse-KO mouse embryonic fibroblasts) suggest that the newly discovered co-regulation of heparanase and MMPs is mediated by stabilization and transcriptional activity of beta-catenin.The lack of heparanase expression and activity was accompanied by alterations in the expression level of MMP family members, primarily MMP-2 and MMP-14. It is conceivable that MMP-2 and MMP-14, which exert some of the effects elicited by heparanase (i.e., over branching of mammary glands, enhanced angiogenic response) can compensate for its absence, in spite of their different enzymatic substrate. Generation of viable Hpse-KO mice lacking significant abnormalities may provide a promising indication for the use of heparanase as a target for drug development.
- Published
- 2009
- Full Text
- View/download PDF
6. Checkpoint inhibitors: Better outcomes among advanced cutaneous head and neck melanoma patients
- Author
-
Michal Lotem, Nir Hirshoren, Nadia Kaplan, Tamar Peretz, Roni Yoeli, Jeffrey M Weinberger, Jonathan Cohen, and Sharon Merims
- Subjects
0301 basic medicine ,Oncology ,Male ,Melanomas ,Skin Neoplasms ,Immune checkpoint inhibitors ,Programmed Cell Death 1 Receptor ,Cancer Treatment ,Metastasis ,0302 clinical medicine ,Antineoplastic Agents, Immunological ,Medicine and Health Sciences ,CTLA-4 Antigen ,Melanoma ,Immune Response ,Response rate (survey) ,Aged, 80 and over ,Multidisciplinary ,Middle Aged ,Treatment Outcome ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Cutaneous Melanoma ,Medicine ,Female ,Anatomy ,Research Article ,Adult ,medicine.medical_specialty ,Adolescent ,Science ,Immunology ,Malignant Skin Neoplasms ,Dermatology ,Lymphatic System ,03 medical and health sciences ,Young Adult ,Internal medicine ,medicine ,Humans ,Aged ,Retrospective Studies ,business.industry ,Cancers and Neoplasms ,Biology and Life Sciences ,Retrospective cohort study ,medicine.disease ,Trunk ,Ipilimumab ,Survival Analysis ,030104 developmental biology ,Cutaneous melanoma ,Lymph Nodes ,business ,Head ,Neck ,Brain metastasis - Abstract
Objective The aim of this study was to investigate if the treatment outcomes of checkpoint inhibitors (CPI) in patients with advanced-stage skin head and neck melanoma (HNM) differs from outcomes in patients with non-HNM. Design A retrospective cohort study of patients with unresectable AJCC stage III and stage IV, who received CPI between 2010 and 2017. Participants Overall, 122 unresectable AJCC stage III and metastatic stage IV melanoma adult patients were treated with CPI during the study period (consecutive patients). The HNM group of patients was comparable with limbs and trunk melanoma group except different distant metastatic (M1a/b/c/d) pattern (p = 0.025). Main outcomes Comparison of overall survival and clinical response to CPI in patients with advanced-stage skin melanoma of the head and neck with non-HNM. Results We analyzed 38 patients with melanoma arising in the head and neck skin regions, 33 with melanoma of limbs and 51 with trunk melanoma. Most of the head and neck patients were men (89.5%), the average age of melanoma diagnosis was 61.4±16.7 years (range 16.4–85.6). More than a third of HNM group of patients (36.8%) were 70 years and older. Overall response rate (ORR) to CPI was 50% (CR 31.6% and PR 18.4%) in the head and neck study group of patients, compared to an ORR of 36.3% and 23.5% in melanoma of the limbs and of the trunk, respectively (p = 0.03). The median overall survival of HNM group of patients was 60.2±6.3 months, CI 95% [47.7–72.7], 63% were alive at 30 months, reaching a plateau. Whereas, the median survival time of limbs and trunk melanoma were 51.2 and 53.4 months, which did not reach significance. Conclusions and relevance Response rate to CPI is significantly improved in patients with melanoma of the head and neck and they have a trend towards improved, long standing, overall survival.
- Published
- 2020
7. Human T Cell Crosstalk Is Induced by Tumor Membrane Transfer
- Author
-
Michal Lotem, Aviad Pato, Shoshana Frankenburg, Roni Engelstein, Sharon Merims, Eitan Yefenof, Arthur Machlenkin, Galit Eisenberg, Ronny Uzana, and Tamar Peretz
- Subjects
Trogocytosis ,T cell ,Antigen presentation ,lcsh:Medicine ,Antigen-Presenting Cells ,chemical and pharmacologic phenomena ,Streptamer ,CD8-Positive T-Lymphocytes ,Biology ,Immunomodulation ,Mice ,Interleukin 21 ,Antigens, Neoplasm ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Cytotoxic T cell ,IL-2 receptor ,lcsh:Science ,Antigen-presenting cell ,Melanoma ,Antigen Presentation ,Multidisciplinary ,lcsh:R ,Cell Membrane ,Histocompatibility Antigens Class II ,Cell biology ,medicine.anatomical_structure ,Immunology ,lcsh:Q ,Female ,Research Article ,T-Lymphocytes, Cytotoxic - Abstract
Trogocytosis is a contact-dependent unidirectional transfer of membrane fragments between immune effector cells and their targets, initially detected in T cells following interaction with professional antigen presenting cells (APC). Previously, we have demonstrated that trogocytosis also takes place between melanoma-specific cytotoxic T lymphocytes (CTLs) and their cognate tumors. In the present study, we took this finding a step further, focusing on the ability of melanoma membrane-imprinted CD8+ T cells to act as APCs (CD8+ T-APCs). We demonstrate that, following trogocytosis, CD8+ T-APCs directly present a variety of melanoma derived peptides to fraternal T cells with the same TCR specificity or to T cells with different TCRs. The resulting T cell-T cell immune synapse leads to (1) Activation of effector CTLs, as determined by proliferation, cytokine secretion and degranulation; (2) Fratricide (killing) of CD8+ T-APCs by the activated CTLs. Thus, trogocytosis enables cross-reactivity among CD8+ T cells with interchanging roles of effectors and APCs. This dual function of tumor-reactive CTLs may hint at their ability to amplify or restrict reactivity against the tumor and participate in modulation of the anti-cancer immune response.
- Published
- 2015
8. Newly Generated Heparanase Knock-Out Mice Unravel Co-Regulation of Heparanase and Matrix Metalloproteinases
- Author
-
Lea Baraz, Xiao Zhang, Ulf Lindahl, Tamar Peretz, Eyal Zcharia, Jin-Ping Li, Israel Vlodavsky, and Juan Jia
- Subjects
MMP2 ,lcsh:Medicine ,Matrix metalloproteinase ,Extracellular matrix ,Mice ,chemistry.chemical_compound ,0302 clinical medicine ,lcsh:Science ,Cells, Cultured ,Glucuronidase ,Mice, Knockout ,Regulation of gene expression ,0303 health sciences ,Multidisciplinary ,Homozygote ,Gene targeting ,Genetics and Genomics/Gene Expression ,Cell Biology/Extra-Cellular Matrix ,Heparan sulfate ,Immunohistochemistry ,3. Good health ,Cell biology ,Genetics and Genomics/Gene Function ,Oncology ,030220 oncology & carcinogenesis ,MMP14 ,Female ,Research Article ,Chromatin Immunoprecipitation ,Heterozygote ,Breast Neoplasms ,Biology ,03 medical and health sciences ,Cell Line, Tumor ,Animals ,Humans ,Heparanase ,RNA, Messenger ,Crosses, Genetic ,030304 developmental biology ,Carcinoma ,lcsh:R ,Fibroblasts ,Embryo, Mammalian ,Molecular biology ,Matrix Metalloproteinases ,Mice, Inbred C57BL ,Gene Expression Regulation ,chemistry ,lcsh:Q - Abstract
Background Heparanase, a mammalian endo-β-D-glucuronidase, specifically degrades heparan sulfate proteoglycans ubiquitously associated with the cell surface and extracellular matrix. This single gene encoded enzyme is over-expressed in most human cancers, promoting tumor metastasis and angiogenesis. Principal Findings We report that targeted disruption of the murine heparanase gene eliminated heparanase enzymatic activity, resulting in accumulation of long heparan sulfate chains. Unexpectedly, the heparanase knockout (Hpse-KO) mice were fertile, exhibited a normal life span and did not show prominent pathological alterations. The lack of major abnormalities is attributed to a marked elevation in the expression of matrix metalloproteinases, for example, MMP2 and MMP14 in the Hpse-KO liver and kidney. Co-regulation of heparanase and MMPs was also noted by a marked decrease in MMP (primarily MMP-2,-9 and 14) expression following transfection and over-expression of the heparanase gene in cultured human mammary carcinoma (MDA-MB-231) cells. Immunostaining (kidney tissue) and chromatin immunoprecipitation (ChIP) analysis (Hpse-KO mouse embryonic fibroblasts) suggest that the newly discovered co-regulation of heparanase and MMPs is mediated by stabilization and transcriptional activity of β-catenin. Conclusions/Significance The lack of heparanase expression and activity was accompanied by alterations in the expression level of MMP family members, primarily MMP-2 and MMP-14. It is conceivable that MMP-2 and MMP-14, which exert some of the effects elicited by heparanase (i.e., over branching of mammary glands, enhanced angiogenic response) can compensate for its absence, in spite of their different enzymatic substrate. Generation of viable Hpse-KO mice lacking significant abnormalities may provide a promising indication for the use of heparanase as a target for drug development.
- Published
- 2009
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