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3 results on '"Toshihiro ICHIJO"'

2. Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.

Author

Chihiro Ochiai, Sonoko Miyauchi, Yuta Kudo, Yuta Naruke, Syuji Yoneyama, Keisuke Tomita, Leng Dongze, Yusuke Chiba, To-Ichi Hirata, Toshihiro Ichijo, Kazuya Nagai, Sota Kobayashi, Shinji Yamada, Hirokazu Hikono, and Kenji Murakami

Subjects
Medicine, Science
Abstract

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.

Published
2021
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3. Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing

Author

Toshihiro Ichijo, Sonoko Miyauchi, Chihiro Ochiai, Syuji Yoneyama, Kazuya Nagai, Yuta Naruke, Yusuke Chiba, Sota Kobayashi, Yuta Kudo, To-ichi Hirata, Leng Dongze, Shinji Yamada, Keisuke Tomita, Kenji Murakami, and Hirokazu Hikono

Subjects
B Cells, Lymphoma, animal diseases, viruses, Artificial Gene Amplification and Extension, Biochemistry, Polymerase Chain Reaction, law.invention, Hematologic Cancers and Related Disorders, White Blood Cells, Proviruses, Animal Cells, immune system diseases, law, Medicine and Health Sciences, Leukemia Virus, Bovine, B-cell lymphoma, Polymerase chain reaction, Mammals, B-Lymphocytes, Multidisciplinary, Bovine leukemia virus, biology, Messenger RNA, Eukaryota, virus diseases, Ruminants, Hematology, Viral Load, Nucleic acids, medicine.anatomical_structure, Oncology, Vertebrates, Medicine, Cellular Types, Research Article, Immune Cells, Science, Immunology, Research and Analysis Methods, Deep sequencing, Bovines, microRNA, Genetics, medicine, Animals, Non-coding RNA, Antibody-Producing Cells, Molecular Biology Techniques, Molecular Biology, B cell, Natural antisense transcripts, Blood Cells, Biology and life sciences, Organisms, Cancers and Neoplasms, Cell Biology, Enzootic Bovine Leukosis, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, medicine.disease, Virology, Gene regulation, MicroRNAs, Amniotes, RNA, Cattle, Gene expression, Zoology
Abstract

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.

Published
2021
Full Text
View/download PDF

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