Search

Your search keyword '"Wilkinson, Mark"' showing total 9 results
Start Over Author "Wilkinson, Mark" Journal plos one
9 results on '"Wilkinson, Mark"'

1. RNAi suppression of xylan synthase genes in wheat starchy endosperm

Author

Wilkinson, Mark D., primary, Kosik, Ondrej, additional, Halsey, Kirstie, additional, Walpole, Hannah, additional, Evans, Jessica, additional, Wood, Abigail J., additional, Ward, Jane L., additional, Mitchell, Rowan A. C., additional, Lovegrove, Alison, additional, and Shewry, Peter R., additional

Published
2021
Full Text
View/download PDF

2. Novel track morphotypes from new tracksites indicate increased Middle Jurassic dinosaur diversity on the Isle of Skye, Scotland

Author

dePolo, Paige E., primary, Brusatte, Stephen L., additional, Challands, Thomas J., additional, Foffa, Davide, additional, Wilkinson, Mark, additional, Clark, Neil D. L., additional, Hoad, Jon, additional, Pereira, Paulo Victor Luiz Gomes da Costa, additional, Ross, Dugald A., additional, and Wade, Thomas J., additional

Published
2020
Full Text
View/download PDF

3. Isolation and purification of recombinant immunoglobulin light chain variable domains from the periplasmic space of Escherichia coli

Author

Hand, Kieran, Wilkinson, Mark C., and Madine, Jillian

Subjects
Physiology, Protein Expression, Immunoglobulin Variable Region, lcsh:Medicine, Pathology and Laboratory Medicine, Biochemistry, Protein Structure, Secondary, Animal Cells, Contaminants, Immune Physiology, Medicine and Health Sciences, Immunoglobulin Light-chain Amyloidosis, Myocytes, Cardiac, lcsh:Science, Materials, Cardiomyocytes, Immune System Proteins, Microscopy, Confocal, Amyloidosis, Optical Lenses, Recombinant Proteins, Optical Equipment, Physical Sciences, Periplasm, Engineering and Technology, Cellular Types, Anatomy, Research Article, Materials Science, Immunology, Muscle Tissue, Equipment, Research and Analysis Methods, Antibodies, Cell Line, Signs and Symptoms, Diagnostic Medicine, Osmotic Shock, Gene Expression and Vector Techniques, Escherichia coli, Animals, Humans, Molecular Biology Techniques, Molecular Biology, Muscle Cells, Molecular Biology Assays and Analysis Techniques, lcsh:R, Biology and Life Sciences, Proteins, Cell Biology, Rats, Biological Tissue, Immunoglobulin Light Chains, lcsh:Q
Abstract

Immunoglobulin light chain amyloidosis is the most common form of systemic amyloidosis. However, very little is known about the underlying mechanisms that initiate and modulate the associated protein aggregation and deposition. Model systems have been established to investigate these disease-associated processes. One of these systems comprises two 114 amino acid light-chain variable domains of the kappa 4 IgG family, SMA and LEN. Despite high sequence identity (93%), SMA is amyloidogenic in vivo, but LEN adopts a stable dimer, displaying amyloidogenic properties only under destabilising conditions in vitro. We present here a refined and reproducible periplasmic expression and purification protocol for SMA and LEN that improves on existing methods and provides high yields of pure protein (10-50mg/L), particularly suitable for structural studies that demand highly concentrated and purified proteins. We confirm that recombinant SMA and LEN proteins have structure and dimerization capabilities consistent with the native proteins and employ fluorescence to probe internalization and cellular localization within cardiomyocytes. We propose periplasmic expression and simplified chromatographic steps outlined here as an optimized method for production of these and other variable light chain domains to investigate the underlying mechanisms of light chain amyloidosis. We show that SMA and LEN can be internalised within cardiomyocytes and were observed to localise to the perinuclear area, assessed by confocal microscopy as a possible mechanism for underlying cytotoxicity and pathogenesis associated with amyloidosis.

Published
2018

4. Correction: Large-scale molecular phylogeny, morphology, divergence-time estimation, and the fossil record of advanced caenophidian snakes (Squamata: Serpentes)

Author

Zaher, Hussam, primary, Murphy, Robert W., additional, Arredondo, Juan Camilo, additional, Graboski, Roberta, additional, Machado-Filho, Paulo Roberto, additional, Mahlow, Kristin, additional, Montingelli, Giovanna G., additional, Quadros, Ana Bottallo, additional, Orlov, Nikolai L., additional, Wilkinson, Mark, additional, Zhang, Ya-Ping, additional, and Grazziotin, Felipe G., additional

Published
2019
Full Text
View/download PDF

5. Large-scale molecular phylogeny, morphology, divergence-time estimation, and the fossil record of advanced caenophidian snakes (Squamata: Serpentes)

Author

Zaher, Hussam, primary, Murphy, Robert W., additional, Arredondo, Juan Camilo, additional, Graboski, Roberta, additional, Machado-Filho, Paulo Roberto, additional, Mahlow, Kristin, additional, Montingelli, Giovanna G., additional, Quadros, Ana Bottallo, additional, Orlov, Nikolai L., additional, Wilkinson, Mark, additional, Zhang, Ya-Ping, additional, and Grazziotin, Felipe G., additional

Published
2019
Full Text
View/download PDF

9. A New Species of Skin-Feeding Caecilian and the First Report of Reproductive Mode in Microcaecilia (Amphibia: Gymnophiona: Siphonopidae).

Author

Wilkinson, Mark, Sherratt, Emma, Starace, Fausto, and Gower, David J.

Subjects
*ANIMAL sexual behavior, *CAECILIANS, *ANIMAL feeding behavior, *COMPARATIVE anatomy, *ANIMAL classification, *HERPETOLOGY, *BIOLOGICAL evolution
Abstract

A new species of siphonopid caecilian, Microcaecilia dermatophagasp. nov., is described based on nine specimens from French Guiana. The new species is the first new caecilian to be described from French Guiana for more than 150 years. It differs from all other Microcaecilia in having fewer secondary annular grooves and/or in lacking a transverse groove on the dorsum of the first collar. Observations of oviparity and of extended parental care in M. dermatophaga are the first reproductive mode data for any species of the genus. Microcaecilia dermatophaga is the third species, and represents the third genus, for which there has been direct observation of young animals feeding on the skin of their attending mother. The species is named for this maternal dermatophagy, which is hypothesised to be characteristic of the Siphonopidae. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results