1. Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays.
- Author
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Foncy J, Estève A, Degache A, Colin C, Dollat X, Cau JC, Vieu C, Trévisiol E, and Malaquin L
- Subjects
- Biosensing Techniques, Cell Adhesion genetics, Cell Shape genetics, Extracellular Matrix Proteins chemistry, Extracellular Matrix Proteins isolation & purification, Microfluidic Analytical Techniques methods, Printing instrumentation, Tissue Array Analysis instrumentation
- Abstract
Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas. This is particularly important when addressing application such as cell microarray manufacturing, which are currently used for a wide range of analytical and pharmaceutical applications. In this paper, we present a large-scale and multiplexed microcontact printing process of extracellular matrix proteins for the fabrication of cell microarrays. We have developed a microfluidic inking approach combined with a magnetic clamping technology that can be adapted to most standard substrates used in biology. We have demonstrated a significant improvement of homogeneity of printed protein patterns on surfaces larger than 1 cm2 through the control of both the flow rate and the wetting mechanism of the stamp surface during microfluidic inking. Thanks to the reproducibility and integration capabilities provided by microfluidics, we have achieved the printing of three different adhesion proteins in one-step transfer. Selective cell adhesion and cell shape adaptation on the produced patterns were observed, showing the suitability of this approach for producing on-demand large-scale cell microarrays., Competing Interests: The study, shown in the submitted article was done into BIOSOFT lab: a joint lab between LAAS-CNRS (an academic lab) and INNOPSYS (a SME dealing with instrumentation). BIOSOFT is funded by the “Agence nationale pour la recherche (ANR)”, www.agence-nationale-recherche.fr and the LABCOM program: ANR-13-LAB2-0009-01. This organization (ANR) did not play a role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript and only provided financial support in the form of authors' salaries and/or research materials. INNOPSYS did not fund the study. The grant was given directly from ANR to the LAAS-CNRS. INNOPSYS provided support in the form of salaries for authors JCC, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author (JCC) was to help for the use of InnoStamp. This commercial affiliation does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2018
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