23 results on '"Xiang, Jin"'
Search Results
2. Rational construction of controllable autoimmune diabetes model depicting clinical features
- Author
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Fan Yu, Xian Zhou, Xiang Jin, Shushu Zhao, Gan Zhao, Sheng Jiang, Shuang Geng, and Bin Wang
- Subjects
Medicine ,Science - Abstract
Through animal models, particularly non-obesity diabetes model (NOD), pathological understandings of human autoimmune diabetes have been gained. However, features of those mouse models and the human disease are not sufficiently analogous; it is therefore not unexpected that interventions based on the mouse data fail at an alarming rate in clinical settings. An improvised model that maximally resembles the real pathological course is highly desirable. Here we devised a ‘double-hit’ strategy, pancreas was first hit by chemical damage (streptozotocin, STZ) to unleash auto-antigens, then hit second time by transient immune-inflammation (regulatory T cell depletion). Comparing to NOD model, this strategy not only induced classical diabetic symptoms, but also depicted the crucial pathogenic features absent in conventional models, such as CD8+ T cell dominant infiltrates, strong ketoacidosis and epitope-specific T cell responses. In addition, this model allowed synchronized control of disease onset, permitting more refined temporal analysis of disease progression. We believe that this model would yield research outcomes with clinically relevant prediction power unattainable previously.
- Published
- 2022
3. Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein S17.
- Author
-
Scott P Kenney and Xiang-Jin Meng
- Subjects
Medicine ,Science - Abstract
Human ribosomal protein S17 (RPS17) is mutated in Diamond-Blackfan Anemia (DBA), a bone marrow disorder that fails to produce sufficient red blood cells leading to anemia. Recently, an RPS17 protein sequence was also found to be naturally inserted in the genome of hepatitis E virus (HEV) from patients chronically-infected by HEV. The role of RPS17 in HEV replication and pathogenesis remains unknown due to the lack of knowledge about how RPS17 functions at a molecular level. Understanding the biological function of RPS17 is critical for elucidating its role in virus infection and DBA disease processes. In this study we probed the subcellular distribution of normal and mutant RPS17 proteins in a human liver cell line (Huh7). RPS17 was primarily detected within the nucleus, and more specifically within the nucleoli. Using a transient expression system in which RPS17 or truncations were expressed as fusions with enhanced yellow fluorescent protein (eYFP), we were able to identify and map, for the first time, two separate nuclear localization signals (NLSs), one to the first 13 amino acids of the amino-terminus of RPS17 and the other within amino acids 30-60. Additionally, we mapped amino acid sequences required for nucleolar accumulation of RPS17 to amino acids 60-70. Amino acids 60-70 possess a di-RG motif that may be necessary for nucleolar retention of RPS17. The results from this study enhance our knowledge of RSP17 and will facilitate future mechanistic studies about the roles of RSP17 in hepatitis E and DBA disease processes.
- Published
- 2015
- Full Text
- View/download PDF
4. Crystal Structure, Cytotoxicity and Interaction with DNA of Zinc (II) Complexes with o-Vanillin Schiff Base Ligands.
- Author
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Mei-Ju Niu, Zhen Li, Guo-Liang Chang, Xiang-Jin Kong, Min Hong, and Qing-fu Zhang
- Subjects
Medicine ,Science - Abstract
Two new zinc complexes, Zn(HL1)2 (1) and [Zn2(H2L2)(OAc)2]2 (2) [H2L1 = Schiff base derived from o-vanillin and (R)-(+)-2-amino-3-phenyl-1-propanol, H3L2 = Schiff base derived from o-vanillin and 2-amino-2-ethyl-1,3-propanediol], have been synthesized and characterized by single crystal X-ray diffraction, elemental analyses, TG analyses, solid fluorescence, IR, UV-Vis and circular dichroism spectra. The structural analysis shows that complex 1 has a right-handed double helical chain along the crystallographic b axis. A homochiral 3D supramolecular architecture has been further constructed by intermolecular C-H··· π, O-H···O and C-H···O interactions. Complex 2 includes two crystallographically independent binuclear zinc molecules. The two binuclear zinc molecules are isostructural. The 2-D sheet supramolecular structure was formed by intermolecular hydrogen bonding interaction. The fluorescence of ligands and complexes in DMF at room temperature are studied. The interactions of two complexes with calf thymus DNA (CT-DNA) are investigated using UV-Vis, CD and fluorescence spectroscopy. The results show that complex 1 exhibits higher interaction with CT-DNA than complex 2. In addition, in vitro cytotoxicity of the complexes towards four kinds of cancerous cell lines (A549, HeLa, HL-60 and K562) were assayed by the MTT method. Investigations on the structures indicated that the chirality and nuclearity of zinc complexes play an important role on cytotoxic activity.
- Published
- 2015
- Full Text
- View/download PDF
5. Proteomics of Fusarium oxysporum race 1 and race 4 reveals enzymes involved in carbohydrate metabolism and ion transport that might play important roles in banana Fusarium wilt.
- Author
-
Yong Sun, Xiaoping Yi, Ming Peng, Huicai Zeng, Dan Wang, Bo Li, Zheng Tong, Lili Chang, Xiang Jin, and Xuchu Wang
- Subjects
Medicine ,Science - Abstract
Banana Fusarium wilt is a soil-spread fungal disease caused by Fusarium oxysporum. In China, the main virulence fungi in banana are F. oxysporum race 1 (F1, weak virulence) and race 4 (F4, strong virulence). To date, no proteomic analyses have compared the two races, but the difference in virulence between F1 and F4 might result from their differentially expressed proteins. Here we report the first comparative proteomics of F1 and F4 cultured under various conditions, and finally identify 99 protein species, which represent 59 unique proteins. These proteins are mainly involved in carbohydrate metabolism, post-translational modification, energy production, and inorganic ion transport. Bioinformatics analysis indicated that among the 46 proteins identified from F4 were several enzymes that might be important for virulence. Reverse transcription PCR analysis of the genes for 15 of the 56 proteins revealed that their transcriptional patterns were similar to their protein expression patterns. Taken together, these data suggest that proteins involved in carbohydrate metabolism and ion transport may be important in the pathogenesis of banana Fusarium wilt. Some enzymes such as catalase-peroxidase, galactosidase and chitinase might contribute to the strong virulence of F4. Overexpression or knockout of the genes for the F4-specific proteins will help us to further understand the molecular mechanism of Fusarium-induced banana wilt.
- Published
- 2014
- Full Text
- View/download PDF
6. Minimally invasive midvastus versus standard parapatellar approach in total knee arthroplasty: a meta-analysis of randomized controlled trials.
- Author
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San-Zhong Xu, Xiang-Jin Lin, Xiang Tong, and Xuan-Wei Wang
- Subjects
Medicine ,Science - Abstract
OBJECTIVE: Minimally invasive midvastus approach (mini-midvastus) has been widely used in total knee arthroplasty (TKA). However, the clinical effects still remains controversial. This meta-analysis was based on randomized controlled trials (RCTs) aiming to quantitatively analyze the clinical efficacy of mini-midvastus versus standard parapatellar approach in TKA. METHODS: This meta-analysis was performed according to the PRISMA guidelines. A literature search for the eligible RCTs was carried out in the databases of PubMed, the Cochrane library, EMBASE and Web of Science. Two independent reviewers independently completed the study selection, data extraction, and the assessment of methodological quality. Meta-analysis was conducted by the RevMan 5.2 software. RESULTS: A total of 18 RCTs (937 patients with 1093 TKAs) published from 2007 to 2013 were included. The meta-analysis suggested that the mini-midvastus approach significantly improved knee range of motion (ROM) and decreased visual analog score (VAS) at postoperative 1-2 weeks (p0.05). However, the operative time was significantly longer when performing the mini-midvastus group than the parapartellar approach (p
- Published
- 2014
- Full Text
- View/download PDF
7. Broadening the heterologous cross-neutralizing antibody inducing ability of porcine reproductive and respiratory syndrome virus by breeding the GP4 or M genes.
- Author
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Lei Zhou, Yan-Yan Ni, Pablo Piñeyro, Caitlin M Cossaboom, Sakthivel Subramaniam, Brenton J Sanford, Barbara A Dryman, Yao-Wei Huang, and Xiang-Jin Meng
- Subjects
Medicine ,Science - Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens, which causes reproductive failure in sows and respiratory disease in piglets. A major hurdle to control PRRSV is the ineffectiveness of the current vaccines to confer protection against heterologous strains. Since both GP4 and M genes of PRRSV induce neutralizing antibodies, in this study we molecularly bred PRRSV through DNA shuffling of the GP4 and M genes, separately, from six genetically different strains of PRRSV in an attempt to identify chimeras with improved heterologous cross-neutralizing capability. The shuffled GP4 and M genes libraries were each cloned into the backbone of PRRSV strain VR2385 infectious clone pIR-VR2385-CA. Three GP4-shuffled chimeras and five M-shuffled chimeras, each representing sequences from all six parental strains, were selected and further characterized in vitro and in pigs. These eight chimeric viruses showed similar levels of replication with their backbone strain VR2385 both in vitro and in vivo, indicating that the DNA shuffling of GP4 and M genes did not significantly impair the replication ability of these chimeras. Cross-neutralization test revealed that the GP4-shuffled chimera GP4TS14 induced significantly higher cross-neutralizing antibodies against heterologous strains FL-12 and NADC20, and similarly that the M-shuffled chimera MTS57 also induced significantly higher levels of cross-neutralizing antibodies against heterologous strains MN184B and NADC20, when compared with their backbone parental strain VR2385 in infected pigs. The results suggest that DNA shuffling of the GP4 or M genes from different parental viruses can broaden the cross-neutralizing antibody-inducing ability of the chimeric viruses against heterologous PRRSV strains. The study has important implications for future development of a broadly protective vaccine against PRRSV.
- Published
- 2013
- Full Text
- View/download PDF
8. A potential role for CHH DNA methylation in cotton fiber growth patterns.
- Author
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Xiang Jin, Yu Pang, Fangxing Jia, Guanghui Xiao, Qin Li, and Yuxian Zhu
- Subjects
Medicine ,Science - Abstract
DNA methylation controls many aspects of plant growth and development. Here, we report a novel annual growth potential change that may correlate with changes in levels of the major DNA demethylases and methyltransferases in cotton ovules harvested at different times of the year. The abundances of DNA demethylases, at both the mRNA and protein levels, increased significantly from February to August and decreased during the remainder of the 12-month period, with the opposite pattern observed for DNA methyltransferases. Over the course of one year, substantial changes in methylcytosine content was observed at certain CHH sites (H = A, C, or T) in the promoter regions of the ETHYLENE RESPONSIVE FACTOR 6 (ERF6), SUPPRESSION OF RVS 161 DELTA 4 (SUR4) and 3-KETOACYL-COA SYNTHASE 13 (KCS13), which regulate cotton fiber growth. Three independent techniques were used to confirm the annual fluctuations in DNA methylation. Furthermore, in homozygous RNAi lines specifically targeting REPRESSOR OF SILENCING 1 (ROS1, a conserved DNA demethylase domain), promotion of DNA methylation significantly reduced fiber growth during August.
- Published
- 2013
- Full Text
- View/download PDF
9. The integrated HIV-1 provirus in patient sperm chromosome and its transfer into the early embryo by fertilization.
- Author
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Dian Wang, Lian-Bing Li, Zhi-Wei Hou, Xiang-Jin Kang, Qing-Dong Xie, Xiao-jun Yu, Ming-Fu Ma, Bo-Lu Ma, Zheng-Song Wang, Yong Lei, and Tian-Hua Huang
- Subjects
Medicine ,Science - Abstract
Complete understanding of the route of HIV-1 transmission is an important prerequisite for curbing the HIV/AIDS pandemic. So far, the known routes of HIV-1 transmission include sexual contact, needle sharing, puncture, transfusion and mother-to-child transmission. Whether HIV can be vertically transmitted from human sperm to embryo by fertilization is largely undetermined. Direct research on embryo derived from infected human sperm and healthy human ova have been difficult because of ethical issues and problems in the collection of ova. However, the use of inter-specific in vitro fertilization (IVF) between human sperm and hamster ova can avoid both of these problems. Combined with molecular, cytogenetical and immunological techniques such as the preparation of human sperm chromosomes, fluorescent in situ hybridization (FISH), and immunofluorescence assay (IFA), this study mainly explored whether any integrated HIV provirus were present in the chromosomes of infected patients' sperm, and whether that provirus could be transferred into early embryos by fertilization and maintain its function of replication and expression. Evidence showed that HIV-1 nucleic acid was present in the spermatozoa of HIV/AIDS patients, that HIV-1 provirus is present on the patient sperm chromosome, that the integrated provirus could be transferred into early embryo chromosomally integrated by fertilization, and that it could replicate alongside the embryonic genome and subsequently express its protein in the embryo. These findings indicate the possibility of vertical transmission of HIV-1 from the sperm genome to the embryonic genome by fertilization. This study also offers a platform for the research into this new mode of transmission for other viruses, especially sexually transmitted viruses.
- Published
- 2011
- Full Text
- View/download PDF
10. Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein S17
- Author
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Xiang-Jin Meng and Scott P. Kenney
- Subjects
Yellow fluorescent protein ,Ribosomal Proteins ,Nucleolus ,Recombinant Fusion Proteins ,Genetic Vectors ,Molecular Sequence Data ,Nuclear Localization Signals ,lcsh:Medicine ,Gene Expression ,03 medical and health sciences ,Protein sequencing ,Bacterial Proteins ,Ribosomal protein ,Cell Line, Tumor ,Humans ,Amino Acid Sequence ,lcsh:Science ,Peptide sequence ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Multidisciplinary ,biology ,030302 biochemistry & molecular biology ,lcsh:R ,Molecular biology ,3. Good health ,Cell biology ,Amino acid ,Luminescent Proteins ,chemistry ,biology.protein ,Hepatocytes ,lcsh:Q ,Nuclear transport ,Nuclear localization sequence ,Cell Nucleolus ,Research Article - Abstract
Human ribosomal protein S17 (RPS17) is mutated in Diamond-Blackfan Anemia (DBA), a bone marrow disorder that fails to produce sufficient red blood cells leading to anemia. Recently, an RPS17 protein sequence was also found to be naturally inserted in the genome of hepatitis E virus (HEV) from patients chronically-infected by HEV. The role of RPS17 in HEV replication and pathogenesis remains unknown due to the lack of knowledge about how RPS17 functions at a molecular level. Understanding the biological function of RPS17 is critical for elucidating its role in virus infection and DBA disease processes. In this study we probed the subcellular distribution of normal and mutant RPS17 proteins in a human liver cell line (Huh7). RPS17 was primarily detected within the nucleus, and more specifically within the nucleoli. Using a transient expression system in which RPS17 or truncations were expressed as fusions with enhanced yellow fluorescent protein (eYFP), we were able to identify and map, for the first time, two separate nuclear localization signals (NLSs), one to the first 13 amino acids of the amino-terminus of RPS17 and the other within amino acids 30-60. Additionally, we mapped amino acid sequences required for nucleolar accumulation of RPS17 to amino acids 60-70. Amino acids 60-70 possess a di-RG motif that may be necessary for nucleolar retention of RPS17. The results from this study enhance our knowledge of RSP17 and will facilitate future mechanistic studies about the roles of RSP17 in hepatitis E and DBA disease processes. Published version
- Published
- 2014
11. Proteomics of Fusarium oxysporum Race 1 and Race 4 Reveals Enzymes Involved in Carbohydrate Metabolism and Ion Transport That Might Play Important Roles in Banana Fusarium Wilt
- Author
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Xiaoping Yi, Lili Chang, Xiang Jin, Huicai Zeng, Yong Sun, Daniel I. C. Wang, Xuchu Wang, Zheng Tong, Ming Peng, and Bo Li
- Subjects
Fusarium ,Proteomics ,Virulence ,lcsh:Medicine ,Plant Science ,Microbiology ,Fungal Proteins ,Gene Expression Regulation, Fungal ,Fusarium oxysporum ,Electrophoresis, Gel, Two-Dimensional ,lcsh:Science ,Gene ,Molecular Biology ,Plant Diseases ,Genetics ,Fungal protein ,Multidisciplinary ,Ion Transport ,biology ,lcsh:R ,food and beverages ,Biology and Life Sciences ,Agriculture ,Musa ,Cell Biology ,biology.organism_classification ,Fusarium wilt ,Chitinase ,biology.protein ,Carbohydrate Metabolism ,lcsh:Q ,Research Article ,Developmental Biology ,Subcellular Fractions - Abstract
Banana Fusarium wilt is a soil–spread fungal disease caused by Fusarium oxysporum. In China, the main virulence fungi in banana are F. oxysporum race 1 (F1, weak virulence) and race 4 (F4, strong virulence). To date, no proteomic analyses have compared the two races, but the difference in virulence between F1 and F4 might result from their differentially expressed proteins. Here we report the first comparative proteomics of F1 and F4 cultured under various conditions, and finally identify 99 protein species, which represent 59 unique proteins. These proteins are mainly involved in carbohydrate metabolism, post-translational modification, energy production, and inorganic ion transport. Bioinformatics analysis indicated that among the 46 proteins identified from F4 were several enzymes that might be important for virulence. Reverse transcription PCR analysis of the genes for 15 of the 56 proteins revealed that their transcriptional patterns were similar to their protein expression patterns. Taken together, these data suggest that proteins involved in carbohydrate metabolism and ion transport may be important in the pathogenesis of banana Fusarium wilt. Some enzymes such as catalase-peroxidase, galactosidase and chitinase might contribute to the strong virulence of F4. Overexpression or knockout of the genes for the F4-specific proteins will help us to further understand the molecular mechanism of Fusarium-induced banana wilt.
- Published
- 2014
12. Influence of alumina binder content on catalytic performance of Ni/HZSM-5 for hydrodeoxygenation of cyclohexanone
- Author
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Junhai Liu and Xiang-Jin Kong
- Subjects
Materials science ,Science ,chemistry.chemical_element ,Cyclohexanone ,Catalysis ,chemistry.chemical_compound ,Structure-Activity Relationship ,Adsorption ,Ammonia ,Nickel ,Desorption ,Industrial Engineering ,Aluminum Oxide ,Multidisciplinary ,Cyclohexanones ,Organic Chemistry ,Applied Chemistry ,Sorption ,Chemical Engineering ,Oxygen ,Chemistry ,chemistry ,Chemical engineering ,Physical Sciences ,Zeolites ,Medicine ,Engineering and Technology ,Dispersion (chemistry) ,Hydrodeoxygenation ,Research Article - Abstract
The influence of the amount of alumina binders on the catalytic performance of Ni/HZSM-5 for hydrodeoxygenation of cyclohexanone was investigated in a fixed-bed reactor. N2 sorption, X-ray diffraction, H2-chemisorption and temperature-programmed desorption of ammonia were used to characterize the catalysts. It can be observed that the Ni/HZSM-5 catalyst bound with 30 wt.% alumina binder exhibited the best catalytic performance. The high catalytic performance may be due to relatively good Ni metal dispersion, moderate mesoporosity, and proper acidity of the catalyst.
- Published
- 2014
13. rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans
- Author
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Chen, Lan, primary, Zhang, Ju, additional, Xu, Jie, additional, Wan, Lu, additional, Teng, Kaixuan, additional, Xiang, Jin, additional, Zhang, Rui, additional, Huang, Zebo, additional, Liu, Yongmei, additional, Li, Wenhua, additional, and Liu, Xin, additional
- Published
- 2016
- Full Text
- View/download PDF
14. Relationship between LTR methylation and gag expression of HIV-1 in human spermatozoa and sperm-derived embryos
- Author
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Tianhua Huang, Lianbing Li, Dian Wang, Jihua Huang, Xiang-Jin Kang, FangZheng Li, Qingdong Xie, Lan Xu, and Ying Zhong
- Subjects
Gene Expression Regulation, Viral ,Male ,Viral Diseases ,Transcription, Genetic ,Zygote ,viruses ,Bisulfite sequencing ,Sexually Transmitted Diseases ,Gene Expression ,lcsh:Medicine ,HIV Infections ,Biology ,Transfection ,Microbiology ,gag Gene Products, Human Immunodeficiency Virus ,Molecular Genetics ,Immunodeficiency Viruses ,Virology ,Cricetinae ,Genetics ,Animals ,Humans ,lcsh:Science ,HIV Long Terminal Repeat ,Regulation of gene expression ,Multidisciplinary ,urogenital system ,lcsh:R ,HIV ,Embryo ,Methylation ,DNA Methylation ,Group-specific antigen ,Spermatozoa ,Sperm ,Molecular biology ,Infectious Diseases ,Protein Biosynthesis ,DNA methylation ,HIV-1 ,Medicine ,Female ,lcsh:Q ,Gene Function ,Viral Transmission and Infection ,Research Article ,Plasmids - Abstract
Objective Studying the methylation status of long terminal repeats (LTR) and its relationship to gag expression of HIV-1 in order to explore regulation mechanism of HIV-1 gene expression in vertical transmission from sperm to embryo. Methods/Principal Findings Sperm samples were collected from a healthy donor and seven patients with HIV/AIDS. Zona-free hamster ova were fertilized by donor’s spermatozoa transfected with pIRES2-EGFP-LTR-gag and patient’s spermatozoa to obtain zygotes and 2-cell embryos, respectively. Interspecific in vitro fertilization, bisulfite sequencing PCR (BSP), RT-PCR, nested RT-PCR, nested real-time qRT-PCR and 2−△△Ct method, indirect immunofluoresence (IF) assay were performed. For donor’s samples, the methylation rates of HIV-1 LTR were 0.56%, 1.67%, 0.56%, 0.56% in plasmid, spermatozoa, zygotes and 2-cell embryos, respectively while spermatozoa were transfected with unmethylated plasmid, and were 95.0%, 84.44%, 3.3%, 1.67% while transfected with methylated plasmid. The positive bands for HIV-1 gag cDNA were detected in spermatozoa and 2-cell embryos. The positive signals for HIV-1 p24 Gag protein were detected in 2-cell embryos but not in spermatozoa. For patient’s samples, methylation rates of HIV-1 LTR were different in spermatozoa among patients. After fertilization, CpG sites in HIV-1 LTR were highly demethylated in zygotes and 2-cell embryos. The gag transcription levels increased with decreasing of methylation rates of HIV-1 LTR, which showed a strong negative correlations between gag transcription levels and methylation rates of HIV-LTR ether in the spermatozoa (r = −0.9877, P
- Published
- 2013
15. The integrated HIV-1 provirus in patient sperm chromosome and its transfer into the early embryo by fertilization
- Author
-
Qing-Dong Xie, Zheng-Song Wang, Yong Lei, Mingfu Ma, Xiao-jun Yu, Tian-Hua Huang, Dian Wang, Bo-Lu Ma, Lianbing Li, Zhiwei Hou, and Xiang-Jin Kang
- Subjects
Male ,Embryology ,Virus Integration ,medicine.medical_treatment ,HIV Core Protein p24 ,Sexually Transmitted Diseases ,Retrovirology and HIV immunopathogenesis ,Fluorescent Antibody Technique ,lcsh:Medicine ,HIV Infections ,Fertilization in Vitro ,Viral diseases ,Biology ,Virus Replication ,gag Gene Products, Human Immunodeficiency Virus ,Genome ,Human fertilization ,Proviruses ,Cricetinae ,medicine ,Animals ,Humans ,Biotinylation ,lcsh:Science ,In Situ Hybridization, Fluorescence ,Ovum ,Cell Nucleus ,Sex Chromosomes ,Multidisciplinary ,In vitro fertilisation ,lcsh:R ,HIV ,Embryo ,Provirus ,Embryo, Mammalian ,Spermatozoa ,Sperm ,Virology ,AIDS ,Viral replication ,pol Gene Products, Human Immunodeficiency Virus ,HIV-1 ,Medicine ,Infectious diseases ,lcsh:Q ,Research Article ,Developmental Biology - Abstract
Complete understanding of the route of HIV-1 transmission is an important prerequisite for curbing the HIV/AIDS pandemic. So far, the known routes of HIV-1 transmission include sexual contact, needle sharing, puncture, transfusion and mother-to-child transmission. Whether HIV can be vertically transmitted from human sperm to embryo by fertilization is largely undetermined. Direct research on embryo derived from infected human sperm and healthy human ova have been difficult because of ethical issues and problems in the collection of ova. However, the use of inter-specific in vitro fertilization (IVF) between human sperm and hamster ova can avoid both of these problems. Combined with molecular, cytogenetical and immunological techniques such as the preparation of human sperm chromosomes, fluorescent in situ hybridization (FISH), and immunofluorescence assay (IFA), this study mainly explored whether any integrated HIV provirus were present in the chromosomes of infected patients' sperm, and whether that provirus could be transferred into early embryos by fertilization and maintain its function of replication and expression. Evidence showed that HIV-1 nucleic acid was present in the spermatozoa of HIV/AIDS patients, that HIV-1 provirus is present on the patient sperm chromosome, that the integrated provirus could be transferred into early embryo chromosomally integrated by fertilization, and that it could replicate alongside the embryonic genome and subsequently express its protein in the embryo. These findings indicate the possibility of vertical transmission of HIV-1 from the sperm genome to the embryonic genome by fertilization. This study also offers a platform for the research into this new mode of transmission for other viruses, especially sexually transmitted viruses.
- Published
- 2011
16. Crystal Structure, Cytotoxicity and Interaction with DNA of Zinc (II) Complexes with o-Vanillin Schiff Base Ligands
- Author
-
Niu, Mei-Ju, primary, Li, Zhen, additional, Chang, Guo-Liang, additional, Kong, Xiang-Jin, additional, Hong, Min, additional, and Zhang, Qing-fu, additional
- Published
- 2015
- Full Text
- View/download PDF
17. Identification and Fine Mapping of Nuclear and Nucleolar Localization Signals within the Human Ribosomal Protein S17
- Author
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Kenney, Scott P., primary and Meng, Xiang-Jin, additional
- Published
- 2015
- Full Text
- View/download PDF
18. Crystal Structure, Cytotoxicity and Interaction with DNA of Zinc (II) Complexes with o-Vanillin Schiff Base Ligands
- Author
-
Xiang-Jin Kong, Min Hong, Zhen Li, Guoliang Chang, Mei-Ju Niu, and Qingfu Zhang
- Subjects
Circular dichroism ,Supramolecular chemistry ,lcsh:Medicine ,chemistry.chemical_element ,HL-60 Cells ,Crystal structure ,Zinc ,Crystallography, X-Ray ,chemistry.chemical_compound ,Humans ,Isostructural ,lcsh:Science ,Schiff Bases ,Multidisciplinary ,Schiff base ,Cytotoxins ,Hydrogen bond ,lcsh:R ,DNA ,Crystallography ,chemistry ,Propylene Glycols ,Benzaldehydes ,lcsh:Q ,K562 Cells ,Chirality (chemistry) ,Research Article - Abstract
Two new zinc complexes, Zn(HL1)2 (1) and [Zn2(H2L2)(OAc)2]2 (2) [H2L1 = Schiff base derived from o-vanillin and (R)-(+)-2-amino-3-phenyl-1-propanol, H3L2 = Schiff base derived from o-vanillin and 2-amino-2-ethyl-1,3-propanediol], have been synthesized and characterized by single crystal X-ray diffraction, elemental analyses, TG analyses, solid fluorescence, IR, UV-Vis and circular dichroism spectra. The structural analysis shows that complex 1 has a right-handed double helical chain along the crystallographic b axis. A homochiral 3D supramolecular architecture has been further constructed by intermolecular C-H··· π, O-H···O and C-H···O interactions. Complex 2 includes two crystallographically independent binuclear zinc molecules. The two binuclear zinc molecules are isostructural. The 2-D sheet supramolecular structure was formed by intermolecular hydrogen bonding interaction. The fluorescence of ligands and complexes in DMF at room temperature are studied. The interactions of two complexes with calf thymus DNA (CT-DNA) are investigated using UV-Vis, CD and fluorescence spectroscopy. The results show that complex 1 exhibits higher interaction with CT-DNA than complex 2. In addition, in vitro cytotoxicity of the complexes towards four kinds of cancerous cell lines (A549, HeLa, HL-60 and K562) were assayed by the MTT method. Investigations on the structures indicated that the chirality and nuclearity of zinc complexes play an important role on cytotoxic activity.
- Published
- 2015
19. Minimally Invasive Midvastus versus Standard Parapatellar Approach in Total Knee Arthroplasty: A Meta-Analysis of Randomized Controlled Trials
- Author
-
Xu, San-Zhong, primary, Lin, Xiang-Jin, additional, Tong, Xiang, additional, and Wang, Xuan-Wei, additional
- Published
- 2014
- Full Text
- View/download PDF
20. Broadening the Heterologous Cross-Neutralizing Antibody Inducing Ability of Porcine Reproductive and Respiratory Syndrome Virus by Breeding the GP4 or M genes
- Author
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Zhou, Lei, primary, Ni, Yan-Yan, additional, Piñeyro, Pablo, additional, Cossaboom, Caitlin M., additional, Subramaniam, Sakthivel, additional, Sanford, Brenton J., additional, Dryman, Barbara A., additional, Huang, Yao-Wei, additional, and Meng, Xiang-Jin, additional
- Published
- 2013
- Full Text
- View/download PDF
21. Hepatitis B Virus S Protein Enhances Sperm Apoptosis and Reduces Sperm Fertilizing Capacity In Vitro
- Author
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Hui Lu, Qingdong Xie, Tianhua Huang, Lan Xu, Xiang-Jin Kang, Jihua Huang, XiaoQin Xu, FangZheng Li, RiRan Wu, Ying Zhong, and XiaoWu Fang
- Subjects
Male ,Gastroenterology and hepatology ,Acrosome reaction ,lcsh:Medicine ,Apoptosis ,Biochemistry ,Hepatitis ,chemistry.chemical_compound ,Adenosine Triphosphate ,Molecular Cell Biology ,lcsh:Science ,Energy-Producing Organelles ,Sperm motility ,Multidisciplinary ,biology ,Hyperactivation ,Cytochrome c ,Cytochromes c ,Hepatitis B ,Spermatozoa ,Cellular Structures ,Adenosine Diphosphate ,Infectious hepatitis ,Sperm Motility ,Medicine ,Infectious diseases ,Cellular Types ,Research Article ,Hepatitis B virus ,endocrine system ,Urology ,Enzyme-Linked Immunosorbent Assay ,Viral diseases ,Bioenergetics ,Viral Proteins ,Humans ,Biology ,Liver diseases ,Infertility, Male ,Zona Pellucida ,urogenital system ,Acrosome Reaction ,lcsh:R ,Molecular biology ,Sperm ,Germ Cells ,Subcellular Organelles ,Mitochondrial permeability transition pore ,chemistry ,Infertility ,biology.protein ,lcsh:Q ,Calcium ,Adenosine triphosphate - Abstract
Objective Studying the impact of Hepatitis B virus S protein (HBs) on early apoptotic events in human spermatozoa and sperm fertilizing capacity. Methodology/principal findings Spermatozoa were exposed to HBs (0, 25, 50, 100 µg/ml) for 3 h, and then fluo-4 AM calcium assay, Calcein/Co(2+) assay, protein extraction and ELISA, ADP/ATP ratio assay, sperm motility and hyperactivation and sperm-zona pellucida (ZP) binding and ZP-induced acrosome reaction (ZPIAR) tests were performed. The results showed that in the spermatozoa, with increasing concentration of HBs, (1) average cytosolic free Ca(2+) concentration ([Ca(2+)]i) rose; (2) fluorescence intensity of Cal-AM declined; (3) average levels of cytochrome c decreased in mitochondrial fraction and increased in cytosolic fraction; (4) ADP/ATP ratios rose; (5) average rates of total motility and mean hyperactivation declined; (6) average rate of ZPIAR declined. In the above groups the effects of HBs exhibited dose dependency. However, there was no significant difference in the number of sperms bound to ZP between the control and all test groups. Conclusion HBs could induce early events in the apoptotic cascade in human spermatozoa, such as elevation of [Ca(2+)]i, opening of mitochondrial permeability transition pore (MPTP), release of cytochrome c (cyt c) and increase of ADP/ATP ratio, but exerted a negative impact on sperm fertilizing capacity.
- Published
- 2013
22. A Potential Role for CHH DNA Methylation in Cotton Fiber Growth Patterns
- Author
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Qin Li, Fangxing Jia, Xiang Jin, Yu Pang, Guanghui Xiao, and Yu-Xian Zhu
- Subjects
Methyltransferase ,lcsh:Medicine ,Plant Science ,Plant Genetics ,Biochemistry ,Polymerase Chain Reaction ,law.invention ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,law ,Plant Genomics ,Promoter Regions, Genetic ,lcsh:Science ,Flowering Plants ,Polymerase chain reaction ,Plant Growth and Development ,Ovule ,Regulation of gene expression ,Multidisciplinary ,biology ,Plants ,Nucleic acids ,Blotting, Southern ,Phenotype ,DNA methylation ,Epigenetics ,RNA Interference ,DNA modification ,Research Article ,Biotechnology ,Molecular Sequence Data ,Repressor ,Genes, Plant ,Genetics ,Cotton Fiber ,Biology ,Transgenic Plants ,Gene ,Gossypium ,Base Sequence ,Gene Expression Profiling ,lcsh:R ,DNA ,Sequence Analysis, DNA ,DNA Methylation ,Molecular biology ,chemistry ,biology.protein ,Demethylase ,Plant Biotechnology ,lcsh:Q ,Developmental Biology - Abstract
DNA methylation controls many aspects of plant growth and development. Here, we report a novel annual growth potential change that may correlate with changes in levels of the major DNA demethylases and methyltransferases in cotton ovules harvested at different times of the year. The abundances of DNA demethylases, at both the mRNA and protein levels, increased significantly from February to August and decreased during the remainder of the 12-month period, with the opposite pattern observed for DNA methyltransferases. Over the course of one year, substantial changes in methylcytosine content was observed at certain CHH sites (H = A, C, or T) in the promoter regions of the ETHYLENE RESPONSIVE FACTOR 6 (ERF6), SUPPRESSION OF RVS 161 DELTA 4 (SUR4) and 3-KETOACYL-COA SYNTHASE 13 (KCS13), which regulate cotton fiber growth. Three independent techniques were used to confirm the annual fluctuations in DNA methylation. Furthermore, in homozygous RNAi lines specifically targeting REPRESSOR OF SILENCING 1 (ROS1, a conserved DNA demethylase domain), promotion of DNA methylation significantly reduced fiber growth during August.
- Published
- 2013
23. The Integrated HIV-1 Provirus in Patient Sperm Chromosome and Its Transfer into the Early Embryo by Fertilization
- Author
-
Wang, Dian, primary, Li, Lian-Bing, additional, Hou, Zhi-Wei, additional, Kang, Xiang-Jin, additional, Xie, Qing-Dong, additional, Yu, Xiao-jun, additional, Ma, Ming-Fu, additional, Ma, Bo-Lu, additional, Wang, Zheng-Song, additional, Lei, Yong, additional, and Huang, Tian-Hua, additional
- Published
- 2011
- Full Text
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