28 results on '"Yang, He"'
Search Results
2. Overexpression of TMEM150A in glioblastoma multiforme patients correlated with dismal prognoses and compromised immune statuses.
- Author
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Si-Tong Fan, Hao-Qiang Xu, Yang He, Ming-Xiang Tu, Ke Shi, Yun-Qiang Zhang, Qiang Guo, Wen-Qiong Yang, and Yong Qin
- Subjects
Medicine ,Science - Abstract
Transmembrane proteins have exhibited a significant correlation with glioblastoma multiforme (GBM). The current study elucidates the roles of transmembrane protein 150A (TMEM150A) in GBM. Data on patients with GBM were collected from The Cancer Genome Atlas and Xena databases. The objective was to identify the expression levels of TMEM150A in patients with GBM, and evaluate its diagnostic and prognostic values, accomplished using the receiver operating characteristic and survival analyses. On a cellular level, Cell Counting Kit-8, Wound healing, and Transwell experiments were performed to gauge the impact of TMEM150A on cell growth and migration. The study further investigated the correlation between TMEM150A expression and immune status, along with ribonucleic acid (RNA) modifications in GBM. The findings demonstrated TMEM150A overexpression in the cancerous tissues of patients with GBM, with an area under the curve value of 0.95. TMEM150A overexpression was significantly correlated with poor prognostic indicators. TMEM150A overexpression and isocitrate dehydrogenase (IDH) mutation status were predictive of poor survival time among patients with GBM. In vitro experiments indicated that suppressing TMEM150A expression could inhibit GBM cell proliferation, migration, and invasion. Moreover, TMEM150A overexpression was associated with stromal, immune, and estimate scores, immune cells (such as the T helper (Th) 17 cells, Th2 cells, and regulatory T cells), cell markers, and RNA modifications. Therefore, TMEM150A overexpression might serve as a promising biomarker for predicting poor prognosis in patients with GBM. Inhibiting TMEM150A expression holds the potential for improving the survival time of patients with GBM.
- Published
- 2023
- Full Text
- View/download PDF
3. In Silico screening of circulating tumor DNA, circulating microRNAs, and long non-coding RNAs as diagnostic molecular biomarkers in ovarian cancer: A comprehensive meta-analysis.
- Author
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Linlin Zhang, Chenyan Hu, Zhongping Huang, Zhijia Li, Qin Zhang, and Yang He
- Subjects
Medicine ,Science - Abstract
BackgroundOvarian cancer (OC) is a leading cause of death in gynecological malignancies worldwide. Multitudinous studies have suggested the potential of circulating tumor DNA (ctDNA), circulating microRNAs (miRNAs), and long non-coding RNAs (lncRNAs) as novel diagnostic molecular biomarkers for OC. Here, we include three updated meta-analysis methods using different molecular biomarkers to evaluate their discriminative value in OC diagnosis.MethodsWe conducted three meta-analyses after searching different databases, and 23 eligible articles, including 8 concerning ctDNA, 11 concerning miRNAs, and 4 concerning lncRNAs, were found. Further, we pooled data concerning the sensitivity, specificity, and other indicators of accuracy for ctDNA/miRNAs/lncRNAs in the diagnosis of OC. The heterogeneity was further explored by meta-regressions and subgroup analyses, and Deeks' funnel plots were used to measure the publication bias of these three meta-analyses.ResultsIn all, this meta-analysis included 1732 OC patients and 3958 controls. The sensitivity of ctDNA for OC diagnosis was superior to that of lncRNA and miRNA (84% vs. 81% vs. 78%). Moreover, the specificity and area under the receiver-operating characteristic (ROC) curve (AUC) of ctDNA were 91% and 94%, which were significantly higher than those of miRNA and lncRNAs (78% and 85%; 78% and 86%, respectively). No significant difference was observed among the two meta-analyses of ctDNA and lncRNA (P > 0.05) with regard to publication bias, while the meta-analysis of miRNA observed a significantly small publication bias (P < 0.05).ConclusionctDNA/miRNAs/lncRNAs may be promising molecular biomarkers for OC diagnosis. Further large-scale studies are needed to verify the potential applicability of ctDNA/miRNAs/lncRNAs molecular signatures alone or in combination as diagnostic molecular biomarkers for OC.
- Published
- 2021
- Full Text
- View/download PDF
4. Crowding in or crowding out? How local government debt influences corporate innovation for China
- Author
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Junbing Xu, Yuanyuan Li, Dawei Feng, Zhouyi Wu, and Yang He
- Subjects
Medicine ,Science - Abstract
The pressure upon local governments to redeem their debt could affect government fiscal ability. It could consequently affect their fiscal policies on corporations, which might distort corporate innovation. Based on the data of Chinese Shanghai and Shenzhen A-share listed companies and the local government implicit short-term debt financed by local government financing vehicles (LGFVs) in 31 provinces, this paper shows that local government debt (LGD) negatively affects corporate R&D investment in China, thereby suggesting a strong crowding-out effect. The crowding-out effect is more pronounced when the firm is a non-state-owned enterprise (NSOE), the firm’s size is small, the firm’s age is young, or the firm is in the lower market competition. This paper provide evidence by interacting the terms that local government actions, such as consumption of fiscal resources, strengthening tax collection efforts, or consumption of credit resources, might partially account for the crowding-out effect. This study illustrates the innovation costs of local government debt.
- Published
- 2021
5. Prevalence of HBsAg among reproductive age couples in Chongqing: A population-based, cross-sectional study
- Author
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Qing Chen, Jun Liu, Yang He, Liu Yang, Huiqiang Luo, Yixi Wang, Xuewen Zhang, and Ningxiu Li
- Subjects
Medicine ,Science - Abstract
Hepatitis B is a leading cause of death worldwide. Here, we performed a large, population-based, cross-sectional study in Chongqing, China from 2011 to 2016 to assess the prevalence of HBsAg among couples of reproductive age, to predict subsequent trends, and to provide evidence for the WHO goal of "the elimination of viral hepatitis as a public health threat by 2030". A total of 386,286 couples aged 20 to 49 years were enrolled in the study. Approximately 14.35% of couples were HBsAg positive, including 95.00% with discordant HBsAg positivity. HBsAg prevalence was higher in men than in women. Among different occupations, the two categories of “houseworker” (female 6.73%, male 9.99%) and “unemployed” (female 6.64%, male 9.94%) showed the highest HBsAg positivity. In different regions, the lowest prevalence appeared in southeastern Chongqing (female 4.87%, male 7.71%). In 2030, the HBsAg positivity rate is expected to be 2.79%, 7.27% and 5.13% in females, males, and the whole population, respectively. According to the trends, this rate would drop to less than 2% in 2034, 2078 and 2051. In conclusion, the HBsAg prevalence in Chongqing is still relatively high compared with that in other parts of western China, especially among reproductive-age men. HBsAg-positive couples should be taken as an important unit of care. Vaccination is necessary before pregnancy if no antibody is found. More attention should be given to people without stable jobs. HBsAg-positive rate will decrease perceptibly by 2030 and will reach the level of low in epidemic areas by 2050.
- Published
- 2021
6. Pre-pregnancy body mass index and time to pregnancy among couples pregnant within a year: A China cohort study.
- Author
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Yuhang Fang, Jun Liu, Yanyan Mao, Yang He, Min Li, Liu Yang, Qianxi Zhu, Qi Tong, and Weijin Zhou
- Subjects
Medicine ,Science - Abstract
BackgroundExtreme pre-pregnancy body mass index (BMI) values have been associated with reduced fecundability and prolonged time to pregnancy in previous studies. However, the effect in fertile couples is unclear.ObjectivesThis study aimed to evaluate the association between pre-pregnancy BMI and fecundability, measured as time to pregnancy (TTP), among couples that achieved pregnancy within 1 year.MethodsThis was a retrospective cohort study of 50,927 couples wishing to conceive, enrolled in the National Free Preconception Health Examination Project (NFPHEP) in Chongqing, China, during 2012-2016. Participants' weight and height were measured by NFPHEP-trained preconception guidance physicians. TTP measured in months was used to determine subfecundity (TTP >6 months). The strength of association between BMI and TTP/subfecundity was measured with fecundability odds ratios (FOR)/odds ratios (OR) and their corresponding 95% confidence intervals (CI), calculated with Cox and logistic regression analysis. We used restricted cubic spline regression (RCS) to test the observed FOR trends.ResultsCompared to women with normal BMI, women with pre-pregnancy overweight/obesity had longer TTP (FOR = 0.96, 95% CI: 0.94-0.99) and increased risk of subfecundity (OR = 1.08, 95% CI: 1.00-1.17). There was no association between TTP and male BMI. RCS trends varied when data were stratified by male pre-pregnancy BMI, with the greatest change detected in pre-pregnancy underweight men.ConclusionsPre-pregnancy overweight/obesity was associated with longer TTP and subfecundity among women who became pregnant within 1 year; this effect was likely mediated by their partners' pre-pregnancy BMI. These findings indicate that BMI could affect fecundability, independently of affecting the risk of sterility. Advice on weight management and maintaining healthy weight should be included in couples' preconception guidance.
- Published
- 2020
- Full Text
- View/download PDF
7. GSK-3β inhibition alleviates arthritis pain via reducing spinal mitochondrial reactive oxygen species level and inflammation
- Author
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Yang, He-Yu, primary, Sun, Xu, additional, Zhen, Shu-Qing, additional, Yu, Liang-Zhu, additional, Ding, Jie-Qiong, additional, Liu, Ling, additional, Xie, Min, additional, and Zhu, Hai-Li, additional
- Published
- 2023
- Full Text
- View/download PDF
8. The Significance of Myriophyllum elatinoides for Swine Wastewater Treatment: Abundance and Community Structure of Ammonia-Oxidizing Microorganisms in Sediments.
- Author
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Xi Li, Miaomiao Zhang, Feng Liu, Yong Li, Yang He, Shunan Zhang, and Jinshui Wu
- Subjects
Medicine ,Science - Abstract
Myriophyllum elatinoides was reported to effectively treat wastewater by removing nitrogen (N) and phosphorus (P). However, little is known about the abundance and community structure of ammonia-oxidizing microorganisms associated with M. elatinoides purification systems. The objective of this research was to characterize the abundance and community structure of ammonia-oxidizing microorganisms in swine wastewater and determine the main nitrogen removal pathways. In this study, five different waters were treated by M. elatinoides in microcosms for one month. The five waters included tap water (Control), swine wastewater (SW), 50% diluted swine wastewater (50% SW), and two synthetic wastewaters: 200 mg NH4+-N L(-1) (200 NH4+-N) and 400 mg NH4+-N L(-1) (400 NH4+-N). The most dramatic changes were in NH4+-N and total N (TN) concentrations, with average removal rates of 84% and 90%, respectively, in the treatments containing swine wastewater. On days 7, 14, and 28, the dissolved oxygen (DO) increased by 81.8%, 210.4% and 136.5%, respectively, compared with on day 0, in the swine wastewater. The results also showed that the bacterial amoA (AOB) copy numbers in the sediments of the treatments were significantly higher than those of archaeal amoA (AOA) copy numbers (p = 0.015). In addition, the high DO concentrations in swine wastewater responded well to the high abundance of AOB. The AOA and AOB community distributions were positively related with NO3-N and were negatively related with DO in swine wastewater treatments. In summary, our experimental results suggested that the M. elatinoides purification system could improve the activity of ammonia-oxidizing microorganisms and consequently might contribute to the significant N removal from the swine wastewater.
- Published
- 2015
- Full Text
- View/download PDF
9. Arrhythmogenic Biophysical Phenotype for SCN5A Mutation S1787N Depends upon Splice Variant Background and Intracellular Acidosis.
- Author
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Rou-Mu Hu, Bi-Hua Tan, David J Tester, Chunhua Song, Yang He, Sinisa Dovat, Blaise Z Peterson, Michael J Ackerman, and Jonathan C Makielski
- Subjects
Medicine ,Science - Abstract
SCN5A is a susceptibility gene for type 3 long QT syndrome, Brugada syndrome, and sudden infant death syndrome. INa dysfunction from mutated SCN5A can depend upon the splice variant background in which it is expressed and also upon environmental factors such as acidosis. S1787N was reported previously as a LQT3-associated mutation and has also been observed in 1 of 295 healthy white controls. Here, we determined the in vitro biophysical phenotype of SCN5A-S1787N in an effort to further assess its possible pathogenicity.We engineered S1787N in the two most common alternatively spliced SCN5A isoforms, the major isoform lacking a glutamine at position 1077 (Q1077del) and the minor isoform containing Q1077, and expressed these two engineered constructs in HEK293 cells for electrophysiological study. Macroscopic voltage-gated INa was measured 24 hours after transfection with standard whole-cell patch clamp techniques. We applied intracellular solutions with pH7.4 or pH6.7. S1787N in the Q1077 background had WT-like INa including peak INa density, activation and inactivation parameters, and late INa amplitude in both pH 7.4 and pH 6.7. However, with S1787N in the Q1077del background, the percentages of INa late/peak were increased by 2.1 fold in pH 7.4 and by 2.9 fold in pH 6.7 when compared to WT.The LQT3-like biophysical phenotype for S1787N depends on both the SCN5A splice variant and on the intracellular pH. These findings provide further evidence that the splice variant and environmental factors affect the molecular phenotype of cardiac SCN5A-encoded sodium channel (Nav1.5), has implications for the clinical phenotype, and may provide insight into acidosis-induced arrhythmia mechanisms.
- Published
- 2015
- Full Text
- View/download PDF
10. Prevalence of HBsAg among reproductive age couples in Chongqing: A population-based, cross-sectional study
- Author
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Xuewen Zhang, Yang He, Hui-Qiang Luo, Liu Yang, Qing Chen, Yixi Wang, Jun Liu, and Ningxiu Li
- Subjects
Male ,HBsAg ,Cross-sectional study ,Gastroenterology and hepatology ,Physiology ,Social Sciences ,Biochemistry ,Hepatitis ,Sociology ,Immune Physiology ,Prevalence ,Ethnicities ,Public and Occupational Health ,Human Families ,Pathology and laboratory medicine ,Cause of death ,education.field_of_study ,Family Characteristics ,Multidisciplinary ,Schools ,Immune System Proteins ,Vaccination ,Hepatitis B ,Middle Aged ,Medical microbiology ,Vaccination and Immunization ,Infectious hepatitis ,Viruses ,Medicine ,Infectious diseases ,Female ,Pathogens ,Viral hepatitis ,Research Article ,Adult ,Medical conditions ,medicine.medical_specialty ,China ,Hepatitis B virus ,Science ,Population ,Immunology ,Viral diseases ,Microbiology ,Antibodies ,Education ,Young Adult ,medicine ,Humans ,Hepatitis B Antibodies ,education ,Liver diseases ,Educational Attainment ,Medicine and health sciences ,Pregnancy ,Hepatitis B Surface Antigens ,Biology and life sciences ,business.industry ,Public health ,Viral pathogens ,Organisms ,Proteins ,medicine.disease ,Hepatitis viruses ,Microbial pathogens ,Cross-Sectional Studies ,People and Places ,Population Groupings ,Preventive Medicine ,business ,Demography - Abstract
Hepatitis B is a leading cause of death worldwide. Here, we performed a large, population-based, cross-sectional study in Chongqing, China from 2011 to 2016 to assess the prevalence of HBsAg among couples of reproductive age, to predict subsequent trends, and to provide evidence for the WHO goal of "the elimination of viral hepatitis as a public health threat by 2030". A total of 386,286 couples aged 20 to 49 years were enrolled in the study. Approximately 14.35% of couples were HBsAg positive, including 95.00% with discordant HBsAg positivity. HBsAg prevalence was higher in men than in women. Among different occupations, the two categories of “houseworker” (female 6.73%, male 9.99%) and “unemployed” (female 6.64%, male 9.94%) showed the highest HBsAg positivity. In different regions, the lowest prevalence appeared in southeastern Chongqing (female 4.87%, male 7.71%). In 2030, the HBsAg positivity rate is expected to be 2.79%, 7.27% and 5.13% in females, males, and the whole population, respectively. According to the trends, this rate would drop to less than 2% in 2034, 2078 and 2051. In conclusion, the HBsAg prevalence in Chongqing is still relatively high compared with that in other parts of western China, especially among reproductive-age men. HBsAg-positive couples should be taken as an important unit of care. Vaccination is necessary before pregnancy if no antibody is found. More attention should be given to people without stable jobs. HBsAg-positive rate will decrease perceptibly by 2030 and will reach the level of low in epidemic areas by 2050.
- Published
- 2021
11. Molecular epidemiology and genetic evolution of the whole genome of G3P[8] human rotavirus in Wuhan, China, from 2000 through 2013.
- Author
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Yuan-Hong Wang, Bei-Bei Pang, Souvik Ghosh, Xuan Zhou, Tsuzumi Shintani, Noriko Urushibara, Yu-Wei Song, Ming-Yang He, Man-Qing Liu, Wei-Feng Tang, Jin-Song Peng, Quan Hu, Dun-Jin Zhou, and Nobumichi Kobayashi
- Subjects
Medicine ,Science - Abstract
Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. Since the latter of the 1990s, G3 human rotaviruses referred to as "new variant G3" have emerged and spread in China, being a dominant genotype until 2010, although their genomic evolution has not yet been well investigated.The complete genomes of 33 G3P[8] human rotavirus strains detected in Wuhan, China, from 2000 through 2013 were analyzed. Phylogenetic trees of concatenated sequences of all the RNA segments and individual genes were constructed together with published rotavirus sequences.Genotypes of 11 gene segments of all the 33 strains were assigned to G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, belonging to Wa genogroup. Phylogenetic analysis of the concatenated full genome sequences indicated that all the modern G3P[8] strains were assigned to Cluster 2 containing only one clade of G3P[8] strains in the US detected in the 1970s, which was distinct from Cluster 1 comprising most of old G3P[8] strains. While main lineages of all the 11 gene segments persisted during the study period, different lineages appeared occasionally in RNA segments encoding VP1, VP4, VP6, and NSP1-NSP5, exhibiting various allele constellations. In contrast, only a single lineage was detected for VP7, VP2, and VP3 genes. Remarkable lineage shift was observed for NSP1 gene; lineage A1-2 emerged in 2007 and became dominant in 2008-2009 epidemic season, while lineage A1-1 persisted throughout the study period.Chinese G3P[8] rotavirus strains have evolved since 2000 by intra-genogroup reassortment with co-circulating strains, accumulating more reassorted genes over the years. This is the first large-scale whole genome-based study to assess the long-term evolution of common human rotaviruses (G3P[8]) in an Asian country.
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- 2014
- Full Text
- View/download PDF
12. Reversal of bortezomib resistance in myelodysplastic syndrome cells by MAPK inhibitors.
- Author
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Yingxing Yue, Ying Wang, Yang He, Shuting Yang, Zixing Chen, Yuanyuan Wang, Shanshan Xing, Congcong Shen, Hesham M Amin, Depei Wu, and Yao-Hua Song
- Subjects
Medicine ,Science - Abstract
The myelodysplastic syndromes (MDS) comprise a heterogeneous group of malignant neoplasms with distinctive clinicopathological features. Currently, there is no specific approach for the treatment of MDS. Here, we report that bortezomib (BTZ), a proteasome inhibitor that has been used to treat plasma cell myeloma, induced G2/M phase cycle arrest in the MDS cell line SKM-1 through upregulation of Wee1, a negative regulator of G2/M phase transition. Treatment by BTZ led to reduced SKM-1 cell viability as well as increased apoptosis and autophagy. The BTZ-induced cell death was associated with reduced expression of p-ERK. To elucidate the implications of downregulation of p-ERK, we established the BTZ resistant cell line SKM-1R. Our data show that resistance to BTZ-induced apoptosis could be reversed by the MEK inhibitors U0126 or PD98059. Our results suggest that MAPK pathway may play an important role in mediating BTZ resistance.
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- 2014
- Full Text
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13. In Silico screening of circulating tumor DNA, circulating microRNAs, and long non-coding RNAs as diagnostic molecular biomarkers in ovarian cancer: A comprehensive meta-analysis
- Author
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Zhongping Huang, Qin Zhang, Zhijia Li, Yang He, Linlin Zhang, and Chenyan Hu
- Subjects
0301 basic medicine ,Oncology ,Biochemistry ,Circulating Tumor DNA ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Medicine and Health Sciences ,Ovarian Neoplasms ,Multidisciplinary ,Statistics ,Metaanalysis ,Ovarian Cancer ,Nucleic acids ,030220 oncology & carcinogenesis ,Meta-analysis ,Area Under Curve ,Long non-coding RNA ,Physical Sciences ,Medicine ,Female ,RNA, Long Noncoding ,Research Article ,medicine.medical_specialty ,Funnel plot ,Science ,In silico ,Research and Analysis Methods ,Sensitivity and Specificity ,03 medical and health sciences ,Diagnostic Medicine ,Internal medicine ,microRNA ,medicine ,Biomarkers, Tumor ,Genetics ,Cancer Detection and Diagnosis ,Humans ,Statistical Methods ,Non-coding RNA ,Natural antisense transcripts ,Biology and life sciences ,business.industry ,Cancers and Neoplasms ,Publication bias ,medicine.disease ,Molecular biomarkers ,Gene regulation ,Circulating MicroRNA ,MicroRNAs ,030104 developmental biology ,ROC Curve ,RNA ,Gene expression ,Ovarian cancer ,business ,Gynecological Tumors ,Biomarkers ,Mathematics - Abstract
Background Ovarian cancer (OC) is a leading cause of death in gynecological malignancies worldwide. Multitudinous studies have suggested the potential of circulating tumor DNA (ctDNA), circulating microRNAs (miRNAs), and long non-coding RNAs (lncRNAs) as novel diagnostic molecular biomarkers for OC. Here, we include three updated meta-analysis methods using different molecular biomarkers to evaluate their discriminative value in OC diagnosis. Methods We conducted three meta-analyses after searching different databases, and 23 eligible articles, including 8 concerning ctDNA, 11 concerning miRNAs, and 4 concerning lncRNAs, were found. Further, we pooled data concerning the sensitivity, specificity, and other indicators of accuracy for ctDNA/miRNAs/lncRNAs in the diagnosis of OC. The heterogeneity was further explored by meta-regressions and subgroup analyses, and Deeks’ funnel plots were used to measure the publication bias of these three meta-analyses. Results In all, this meta-analysis included 1732 OC patients and 3958 controls. The sensitivity of ctDNA for OC diagnosis was superior to that of lncRNA and miRNA (84% vs. 81% vs. 78%). Moreover, the specificity and area under the receiver-operating characteristic (ROC) curve (AUC) of ctDNA were 91% and 94%, which were significantly higher than those of miRNA and lncRNAs (78% and 85%; 78% and 86%, respectively). No significant difference was observed among the two meta-analyses of ctDNA and lncRNA (P > 0.05) with regard to publication bias, while the meta-analysis of miRNA observed a significantly small publication bias (P < 0.05). Conclusion ctDNA/miRNAs/lncRNAs may be promising molecular biomarkers for OC diagnosis. Further large-scale studies are needed to verify the potential applicability of ctDNA/miRNAs/lncRNAs molecular signatures alone or in combination as diagnostic molecular biomarkers for OC.
- Published
- 2020
14. FliC, a flagellin protein, is essential for the growth and virulence of fish pathogen Edwardsiella tarda.
- Author
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Yang He, Tingting Xu, Leif E Fossheim, and Xiao-Hua Zhang
- Subjects
Medicine ,Science - Abstract
Edwardsiella tarda is a flagellated gram-negative bacterium which causes edwardsiellosis in fish. FliC, as a flagellar filament structural protein, is hypothesized to be involved in the pathogenesis of infection. In this study, a fliC in-frame deletion mutant of a virulent isolate of E. tarda was constructed through double crossover allelic exchange by means of the suicide vector pRE112, and its virulence-associated phenotypes and pathogenicity were tested. It was found that the deletion of fliC significantly decreased the diameter of flagella filaments. In addition, the mutant showed reduced pathogenicity to fish by increasing the LD(50) value for 100-fold compared to the wild-type strain, as well as showed impaired bacterial growth, reduced motility, decreased biofilm formation and reduced levels of virulence-associated protein secretion involved in the type III secretion system (TTSS). The phenotypic characteristics of the fliC deletion mutant uncovered in this investigation suggest that fliC plays an essential role in normal flagellum function, bacterial growth, protein secretion by TTSS and bacterial virulence.
- Published
- 2012
- Full Text
- View/download PDF
15. Pre-pregnancy body mass index and time to pregnancy among couples pregnant within a year: A China cohort study
- Author
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Yanyan Mao, Yang He, Qi Tong, Liu Yang, Jun Liu, Yuhang Fang, Qian-Xi Zhu, Weijin Zhou, and Min Li
- Subjects
Male ,Physiology ,Maternal Health ,Social Sciences ,Overweight ,Miscarriage ,Body Mass Index ,Cohort Studies ,Mathematical and Statistical Techniques ,Pregnancy ,Weight management ,Medicine and Health Sciences ,Psychology ,Multidisciplinary ,Alcohol Consumption ,Obstetrics ,Statistics ,Obstetrics and Gynecology ,Middle Aged ,Physiological Parameters ,Research Design ,Physical Sciences ,Medicine ,Regression Analysis ,Female ,medicine.symptom ,Underweight ,Cohort study ,Research Article ,Adult ,medicine.medical_specialty ,China ,Science ,Psychological Stress ,Research and Analysis Methods ,Young Adult ,Mental Health and Psychiatry ,medicine ,Humans ,Obesity ,Statistical Methods ,Aged ,Retrospective Studies ,Nutrition ,business.industry ,Body Weight ,Biology and Life Sciences ,Retrospective cohort study ,Odds ratio ,medicine.disease ,Diet ,Pregnancy Complications ,Time-to-Pregnancy ,Infertility ,Women's Health ,business ,Body mass index ,Mathematics - Abstract
BackgroundExtreme pre-pregnancy body mass index (BMI) values have been associated with reduced fecundability and prolonged time to pregnancy in previous studies. However, the effect in fertile couples is unclear.ObjectivesThis study aimed to evaluate the association between pre-pregnancy BMI and fecundability, measured as time to pregnancy (TTP), among couples that achieved pregnancy within 1 year.MethodsThis was a retrospective cohort study of 50,927 couples wishing to conceive, enrolled in the National Free Preconception Health Examination Project (NFPHEP) in Chongqing, China, during 2012-2016. Participants' weight and height were measured by NFPHEP-trained preconception guidance physicians. TTP measured in months was used to determine subfecundity (TTP >6 months). The strength of association between BMI and TTP/subfecundity was measured with fecundability odds ratios (FOR)/odds ratios (OR) and their corresponding 95% confidence intervals (CI), calculated with Cox and logistic regression analysis. We used restricted cubic spline regression (RCS) to test the observed FOR trends.ResultsCompared to women with normal BMI, women with pre-pregnancy overweight/obesity had longer TTP (FOR = 0.96, 95% CI: 0.94-0.99) and increased risk of subfecundity (OR = 1.08, 95% CI: 1.00-1.17). There was no association between TTP and male BMI. RCS trends varied when data were stratified by male pre-pregnancy BMI, with the greatest change detected in pre-pregnancy underweight men.ConclusionsPre-pregnancy overweight/obesity was associated with longer TTP and subfecundity among women who became pregnant within 1 year; this effect was likely mediated by their partners' pre-pregnancy BMI. These findings indicate that BMI could affect fecundability, independently of affecting the risk of sterility. Advice on weight management and maintaining healthy weight should be included in couples' preconception guidance.
- Published
- 2019
16. Crowding in or crowding out? How local government debt influences corporate innovation for China
- Author
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Yuanyuan Li, Zhouyi Wu, Junbing Xu, Yang He, and Dawei Feng
- Subjects
China ,Financing, Government ,Economics ,Science ,Political Science ,media_common.quotation_subject ,Social Sciences ,Local Governments ,Public Policy ,Financial system ,Research and Analysis Methods ,Instrumental Variable Analysis ,Creativity ,Governments ,Mathematical and Statistical Techniques ,Development Economics ,Economic Growth ,Debt ,Humans ,Economic impact analysis ,Investments ,Statistical Methods ,media_common ,Consumption (economics) ,Government ,Local Government ,Multidisciplinary ,Statistics ,Commerce ,Taxes ,Investment (macroeconomics) ,Economic Analysis ,Crowding out ,Fiscal policy ,Fiscal Policy ,Public Finance ,Economic Impact Analysis ,Local government ,Physical Sciences ,Money Supply and Banking ,Medicine ,Business ,Finance ,Mathematics ,Research Article - Abstract
The pressure upon local governments to redeem their debt could affect government fiscal ability. It could consequently affect their fiscal policies on corporations, which might distort corporate innovation. Based on the data of Chinese Shanghai and Shenzhen A-share listed companies and the local government implicit short-term debt financed by local government financing vehicles (LGFVs) in 31 provinces, this paper shows that local government debt (LGD) negatively affects corporate R&D investment in China, thereby suggesting a strong crowding-out effect. The crowding-out effect is more pronounced when the firm is a non-state-owned enterprise (NSOE), the firm’s size is small, the firm’s age is young, or the firm is in the lower market competition. This paper provide evidence by interacting the terms that local government actions, such as consumption of fiscal resources, strengthening tax collection efforts, or consumption of credit resources, might partially account for the crowding-out effect. This study illustrates the innovation costs of local government debt.
- Published
- 2021
17. Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew
- Author
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Guo, Wei-Li, primary, Chen, Bi-Hua, additional, Chen, Xue-Jin, additional, Guo, Yan-Yan, additional, Yang, He-Lian, additional, Li, Xin-Zheng, additional, and Wang, Guang-Yin, additional
- Published
- 2018
- Full Text
- View/download PDF
18. The Significance of Myriophyllum elatinoides for Swine Wastewater Treatment: Abundance and Community Structure of Ammonia-Oxidizing Microorganisms in Sediments
- Author
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Yong Li, Feng Liu, Miaomiao Zhang, Jinshui Wu, Yang He, Shunan Zhang, and Xi Li
- Subjects
Geologic Sediments ,Nitrogen ,Swine ,Microorganism ,Archaeal Proteins ,chemistry.chemical_element ,lcsh:Medicine ,Wastewater ,Magnoliopsida ,Tap water ,Ammonia ,Animals ,lcsh:Science ,Nitrogen cycle ,Ecosystem ,Multidisciplinary ,Myriophyllum ,biology ,Bacteria ,Ecology ,Phosphorus ,lcsh:R ,biology.organism_classification ,Archaea ,chemistry ,Environmental chemistry ,Nitrification ,lcsh:Q ,Microcosm ,Oxidoreductases ,Oxidation-Reduction ,Research Article - Abstract
Myriophyllum elatinoides was reported to effectively treat wastewater by removing nitrogen (N) and phosphorus (P). However, little is known about the abundance and community structure of ammonia-oxidizing microorganisms associated with M. elatinoides purification systems. The objective of this research was to characterize the abundance and community structure of ammonia-oxidizing microorganisms in swine wastewater and determine the main nitrogen removal pathways. In this study, five different waters were treated by M. elatinoides in microcosms for one month. The five waters included tap water (Control), swine wastewater (SW), 50% diluted swine wastewater (50% SW), and two synthetic wastewaters: 200 mg NH4 +-N L−1 (200 NH4 +-N) and 400 mg NH4 +-N L−1 (400 NH4 +-N). The most dramatic changes were in NH4 +-N and total N (TN) concentrations, with average removal rates of 84% and 90%, respectively, in the treatments containing swine wastewater. On days 7, 14, and 28, the dissolved oxygen (DO) increased by 81.8%, 210.4% and 136.5%, respectively, compared with on day 0, in the swine wastewater. The results also showed that the bacterial amoA (AOB) copy numbers in the sediments of the treatments were significantly higher than those of archaeal amoA (AOA) copy numbers (p = 0.015). In addition, the high DO concentrations in swine wastewater responded well to the high abundance of AOB. The AOA and AOB community distributions were positively related with NO3 -N and were negatively related with DO in swine wastewater treatments. In summary, our experimental results suggested that the M. elatinoides purification system could improve the activity of ammonia-oxidizing microorganisms and consequently might contribute to the significant N removal from the swine wastewater.
- Published
- 2015
19. Arrhythmogenic Biophysical Phenotype for SCN5A Mutation S1787N Depends upon Splice Variant Background and Intracellular Acidosis
- Author
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Michael J. Ackerman, Bi-Hua Tan, Rou Mu Hu, Jonathan C. Makielski, Blaise Z. Peterson, Chunhua Song, Yang He, David J. Tester, and Sinisa Dovat
- Subjects
Gene isoform ,Patch-Clamp Techniques ,Intracellular pH ,Long QT syndrome ,RNA Splicing ,lcsh:Medicine ,Biology ,medicine.disease_cause ,Polymorphism, Single Nucleotide ,Membrane Potentials ,NAV1.5 Voltage-Gated Sodium Channel ,Cardiac Conduction System Disease ,medicine ,Humans ,Protein Isoforms ,lcsh:Science ,Brugada syndrome ,Mutation ,Multidisciplinary ,Base Sequence ,lcsh:R ,Arrhythmias, Cardiac ,Sudden infant death syndrome ,Hydrogen-Ion Concentration ,medicine.disease ,Phenotype ,Molecular biology ,Long QT Syndrome ,HEK293 Cells ,Mutagenesis, Site-Directed ,lcsh:Q ,Acidosis ,Intracellular ,Research Article - Abstract
Background SCN5A is a susceptibility gene for type 3 long QT syndrome, Brugada syndrome, and sudden infant death syndrome. INa dysfunction from mutated SCN5A can depend upon the splice variant background in which it is expressed and also upon environmental factors such as acidosis. S1787N was reported previously as a LQT3-associated mutation and has also been observed in 1 of 295 healthy white controls. Here, we determined the in vitro biophysical phenotype of SCN5A-S1787N in an effort to further assess its possible pathogenicity. Methods and Results We engineered S1787N in the two most common alternatively spliced SCN5A isoforms, the major isoform lacking a glutamine at position 1077 (Q1077del) and the minor isoform containing Q1077, and expressed these two engineered constructs in HEK293 cells for electrophysiological study. Macroscopic voltage-gated INa was measured 24 hours after transfection with standard whole-cell patch clamp techniques. We applied intracellular solutions with pH7.4 or pH6.7. S1787N in the Q1077 background had WT-like INa including peak INa density, activation and inactivation parameters, and late INa amplitude in both pH 7.4 and pH 6.7. However, with S1787N in the Q1077del background, the percentages of INa late/peak were increased by 2.1 fold in pH 7.4 and by 2.9 fold in pH 6.7 when compared to WT. Conclusion The LQT3-like biophysical phenotype for S1787N depends on both the SCN5A splice variant and on the intracellular pH. These findings provide further evidence that the splice variant and environmental factors affect the molecular phenotype of cardiac SCN5A-encoded sodium channel (Nav1.5), has implications for the clinical phenotype, and may provide insight into acidosis-induced arrhythmia mechanisms.
- Published
- 2015
20. Molecular Epidemiology and Genetic Evolution of the Whole Genome of G3P[8] Human Rotavirus in Wuhan, China, from 2000 through 2013
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Xuan Zhou, Yu-Wei Song, Dun-Jin Zhou, Noriko Urushibara, Yuan-Hong Wang, Nobumichi Kobayashi, Quan Hu, Wei-Feng Tang, Souvik Ghosh, Bei-Bei Pang, Tsuzumi Shintani, Jin-Song Peng, Man-Qing Liu, and Ming-Yang He
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Evolutionary Genetics ,Rotavirus ,Viral Diseases ,Genes, Viral ,Epidemiology ,viruses ,lcsh:Medicine ,medicine.disease_cause ,Genome ,Gene Frequency ,Genotype ,Medicine and Health Sciences ,Prevalence ,lcsh:Science ,Genome Evolution ,Phylogeny ,Genetics ,Molecular Epidemiology ,Multidisciplinary ,virus diseases ,Phylogenetics ,Infectious Diseases ,Phenotype ,RNA, Viral ,Seasons ,Research Article ,Genome evolution ,China ,Gastroenterology and Hepatology ,Genome, Viral ,Biology ,Microbiology ,History, 21st Century ,Viral Evolution ,Rotavirus Infections ,Evolution, Molecular ,Spatio-Temporal Analysis ,Virology ,medicine ,Humans ,Evolutionary Systematics ,Rotavirus Infection ,Evolutionary Biology ,Molecular epidemiology ,Human evolutionary genetics ,lcsh:R ,Biology and Life Sciences ,Computational Biology ,Genetic Variation ,Sequence Analysis, DNA ,Organismal Evolution ,Amino Acid Substitution ,Microbial Evolution ,lcsh:Q - Abstract
Background Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. Since the latter of the 1990s, G3 human rotaviruses referred to as “new variant G3” have emerged and spread in China, being a dominant genotype until 2010, although their genomic evolution has not yet been well investigated. Methods The complete genomes of 33 G3P[8] human rotavirus strains detected in Wuhan, China, from 2000 through 2013 were analyzed. Phylogenetic trees of concatenated sequences of all the RNA segments and individual genes were constructed together with published rotavirus sequences. Results Genotypes of 11 gene segments of all the 33 strains were assigned to G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, belonging to Wa genogroup. Phylogenetic analysis of the concatenated full genome sequences indicated that all the modern G3P[8] strains were assigned to Cluster 2 containing only one clade of G3P[8] strains in the US detected in the 1970s, which was distinct from Cluster 1 comprising most of old G3P[8] strains. While main lineages of all the 11 gene segments persisted during the study period, different lineages appeared occasionally in RNA segments encoding VP1, VP4, VP6, and NSP1-NSP5, exhibiting various allele constellations. In contrast, only a single lineage was detected for VP7, VP2, and VP3 genes. Remarkable lineage shift was observed for NSP1 gene; lineage A1-2 emerged in 2007 and became dominant in 2008–2009 epidemic season, while lineage A1-1 persisted throughout the study period. Conclusion Chinese G3P[8] rotavirus strains have evolved since 2000 by intra-genogroup reassortment with co-circulating strains, accumulating more reassorted genes over the years. This is the first large-scale whole genome-based study to assess the long-term evolution of common human rotaviruses (G3P[8]) in an Asian country.
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- 2014
21. Normalization for Relative Quantification of mRNA and microRNA in Soybean Exposed to Various Abiotic Stresses
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Liu, Weican, primary, Deng, Yu, additional, Zhou, Yonggang, additional, Chen, Huan, additional, Dong, Yuanyuan, additional, Wang, Nan, additional, Li, Xiaowei, additional, Jameel, Aysha, additional, Yang, He, additional, Zhang, Min, additional, Chen, Kai, additional, Wang, Fawei, additional, and Li, Haiyan, additional
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- 2016
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22. FliC, a Flagellin Protein, Is Essential for the Growth and Virulence of Fish Pathogen Edwardsiella tarda
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Tingting Xu, Yang He, Xiao-Hua Zhang, and Leif E. Fossheim
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Virulence Factors ,Mutant ,lcsh:Medicine ,Virulence ,Aquaculture ,Pathogenesis ,Flagellum ,Microbiology ,Type three secretion system ,Fish Diseases ,Bacterial Proteins ,Nucleic Acids ,Molecular Cell Biology ,Genetics ,Gram Negative ,Animals ,lcsh:Science ,Pathogen ,Biology ,Edwardsiella tarda ,Multidisciplinary ,biology ,lcsh:R ,Enterobacteriaceae Infections ,Agriculture ,Bacteriology ,biology.organism_classification ,Bacterial Pathogens ,Bacterial Biochemistry ,Secretory protein ,Flagella ,Biofilms ,Mutation ,biology.protein ,bacteria ,lcsh:Q ,Mariculture ,Gene Function ,Zoology ,Flagellin ,Ichthyology ,Research Article - Abstract
Edwardsiella tarda is a flagellated gram-negative bacterium which causes edwardsiellosis in fish. FliC, as a flagellar filament structural protein, is hypothesized to be involved in the pathogenesis of infection. In this study, a fliC in-frame deletion mutant of a virulent isolate of E. tarda was constructed through double crossover allelic exchange by means of the suicide vector pRE112, and its virulence-associated phenotypes and pathogenicity were tested. It was found that the deletion of fliC significantly decreased the diameter of flagella filaments. In addition, the mutant showed reduced pathogenicity to fish by increasing the LD(50) value for 100-fold compared to the wild-type strain, as well as showed impaired bacterial growth, reduced motility, decreased biofilm formation and reduced levels of virulence-associated protein secretion involved in the type III secretion system (TTSS). The phenotypic characteristics of the fliC deletion mutant uncovered in this investigation suggest that fliC plays an essential role in normal flagellum function, bacterial growth, protein secretion by TTSS and bacterial virulence.
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- 2012
23. Deregulated HOXB7 Expression Predicts Poor Prognosis of Patients with Esophageal Squamous Cell Carcinoma and Regulates Cancer Cell Proliferation In Vitro and In Vivo
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Li, Hui, primary, Shen, Lu-Yan, additional, Yan, Wan-Pu, additional, Dong, Bin, additional, Kang, Xiao-Zheng, additional, Dai, Liang, additional, Yang, Yong-Bo, additional, Fu, Hao, additional, Yang, He-Li, additional, Zhou, Hai-Tao, additional, Huang, Chuan, additional, Liang, Zhen, additional, Xiong, Hong-Chao, additional, and Chen, Ke-Neng, additional
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- 2015
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24. Calcium Sensing Receptor Modulates Extracellular Calcium Entry and Proliferation via TRPC3/6 Channels in Cultured Human Mesangial Cells
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Meng, Kexin, primary, Xu, Jia, additional, Zhang, Chengwei, additional, Zhang, Rui, additional, Yang, He, additional, Liao, Chang, additional, and Jiao, Jundong, additional
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- 2014
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25. The Use of Functional Chemical-Protein Associations to Identify Multi-Pathway Renoprotectants
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Xu, Jia, primary, Meng, Kexin, additional, Zhang, Rui, additional, Yang, He, additional, Liao, Chang, additional, Zhu, Wenliang, additional, and Jiao, Jundong, additional
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- 2014
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26. Uncovering the Genetic Landscape for Multiple Sleep-Wake Traits
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Winrow, Christopher J., primary, Williams, Deanna L., additional, Kasarskis, Andrew, additional, Millstein, Joshua, additional, Laposky, Aaron D., additional, Yang, He S., additional, Mrazek, Karrie, additional, Zhou, Lili, additional, Owens, Joseph R., additional, Radzicki, Daniel, additional, Preuss, Fabian, additional, Schadt, Eric E., additional, Shimomura, Kazuhiro, additional, Vitaterna, Martha H., additional, Zhang, Chunsheng, additional, Koblan, Kenneth S., additional, Renger, John J., additional, and Turek, Fred W., additional
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- 2009
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27. Uncovering the Genetic Landscape for Multiple Sleep- Wake Traits.
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,4Winrow, Christopher J., Williams, Deanna L., Kasarskis, Andrew, Millstein, Joshua, Laposky, Aaron D., Yang, He S., Mrazek, Karrie, Zhou, Lili, Owens, Joseph R., Radzicki, Daniel, Preuss, Fabian, Schadt, Eric E., Shimomura, Kazuhiro, Vitaterna, Martha H., Chunsheng Zhang, Koblan, Kenneth S., Renger, John J., and Turek, Fred W.
- Subjects
SLEEP physiology ,GENETIC regulation ,CHROMOSOME abnormalities ,LOCUS (Genetics) ,CELL nuclei - Abstract
Despite decades of research in defining sleep-wake properties in mammals, little is known about the nature or identity of genes that regulate sleep, a fundamental behaviour that in humans occupies about one-third of the entire lifespan. While genome-wide association studies in humans and quantitative trait loci (QTL) analyses in mice have identified candidate genes for an increasing number of complex traits and genetic diseases, the resources and time-consuming process necessary for obtaining detailed quantitative data have made sleep seemingly intractable to similar large-scale genomic approaches. Here we describe analysis of 20 sleep-wake traits from 269 mice from a genetically segregating population that reveals 52 significant QTL representing a minimum of 20 genomic loci. While many (28) QTL affected a particular sleep-wake trait (e.g., amount of wake) across the full 24-hr day, other loci only affected a trait in the light or dark period while some loci had opposite effects on the trait during the light vs. dark. Analysis of a dataset for multiple sleep-wake traits led to previously undetected interactions (including the differential genetic control of number and duration of REM bouts), as well as possible shared genetic regulatory mechanisms for seemingly different unrelated sleep-wake traits (e.g., number of arousals and REM latency). Construction of a Bayesian network for sleep-wake traits and loci led to the identification of subnetworks of linkage not detectable in smaller data sets or limited single-trait analyses. For example, the network analyses revealed a novel chain of causal relationships between the chromosome 17@29cM QTL, total amount of wake, and duration of wake bouts in both light and dark periods that implies a mechanism whereby overall sleep need, mediated by this locus, in turn determines the length of each wake bout. Taken together, the present results reveal a complex genetic landscape underlying multiple sleep-wake traits and emphasize the need for a systems biology approach for elucidating the full extent of the genetic regulatory mechanisms of this complex and universal behavior. [ABSTRACT FROM AUTHOR]
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- 2009
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28. Effect of Co-Composting Cattle Manure with Construction and Demolition Waste on the Archaeal, Bacterial, and Fungal Microbiota, and on Antimicrobial Resistance Determinants.
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Holman DB, Hao X, Topp E, Yang HE, and Alexander TW
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- Agriculture, Animals, Archaea genetics, Archaea isolation & purification, Bacteria genetics, Bacteria isolation & purification, Cattle, Drug Resistance, Microbial, Fungi genetics, Fungi isolation & purification, Manure analysis, RNA, Ribosomal, 16S genetics, Manure microbiology, Microbiota, Soil Microbiology, Solid Waste analysis
- Abstract
Agricultural operations generate large quantities of manure which must be eliminated in a manner that is consistent with public health guidelines. Meanwhile, construction and demolition waste makes up about 25% of total solid municipal waste. Co-composting of manure with construction and demolition waste offers a potential means to make manure safe for soil amendment and also divert construction and demolition waste from municipal landfills. Therefore, the archaeal, bacterial, and fungal microbiota of two different types of composted cattle manure and one co-composted with construction and demolition waste, were assessed over a 99-day composting period. The microbiota of the three compost mixtures did not differ, but significant changes over time and by sampling depth were observed. Bacillus and Halocella, however, were more relatively abundant in composted manure from cattle fed dried distillers' grains and solubles. Proteobacteria and Bacteroidetes were enriched at day 0 and Firmicutes at day 99. The fungal genus Kernia was the most relatively abundant overall and was enriched at day 0. The concentration of 12 antimicrobial resistance determinants in the compost mixtures was also determined, and 10 of these determinants decreased significantly from days 0 to 99. The addition of construction and demolition waste did not affect the persistence of antimicrobial resistance genes or community structure of the compost microbiota and therefore co-composting construction and demolition waste with cattle manure offers a safe, viable way to divert this waste from landfills.
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- 2016
- Full Text
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