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17 results on '"Yang, Shang"'

4. Elevated serum galectin-1 concentrations are associated with increased risks of mortality and acute kidney injury in critically ill patients

Author

Chou, Ruey-Hsing, primary, Tsai, Chuan-Tsai, additional, Lu, Ya-Wen, additional, Guo, Jiun-Yu, additional, Lu, Chi-Ting, additional, Tsai, Yi-Lin, additional, Wu, Cheng-Hsueh, additional, Lin, Shing-Jong, additional, Lien, Ru-Yu, additional, Lu, Shu-Fen, additional, Yang, Shang-Feng, additional, and Huang, Po-Hsun, additional

Published
2021
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10. Presence of Tablet Remnants of Nevirapine Extended-Release in Stools and Its Impact on Virological Outcome in HIV-1-Infected Patients: A Prospective Cohort Study

Author

Lee, Yi-Chieh, primary, Lin, Shu-Wen, additional, Chen, Mao-Yuan, additional, Chang, Sui-Yuan, additional, Kuo, Ching-Hua, additional, Sheng, Wang-Huei, additional, Hsieh, Szu-Min, additional, Sun, Hsin-Yun, additional, Chang, Hsi-Yen, additional, Wu, Mon-Ro, additional, Liu, Wen-Chun, additional, Wu, Pei-Ying, additional, Yang, Shang-Ping, additional, Zhang, Jun-Yu, additional, Su, Yi-Ching, additional, Luo, Yi-Zhen, additional, Hung, Chien-Ching, additional, and Chang, Shan-Chwen, additional

Published
2015
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17. Improved Production of 2,3-Butanediol in Bacillus amyloliquefaciens by Over-Expression of Glyceraldehyde-3-Phosphate Dehydrogenase and 2,3-butanediol Dehydrogenase.

Author

Yang, Taowei, Rao, Zhiming, Zhang, Xian, Xu, Meijuan, Xu, Zhenghong, and Yang, Shang-Tian

Subjects
BUTANEDIOL, GLYCERALDEHYDEPHOSPHATE dehydrogenase, GENE expression, FERMENTATION, ACETOIN, LACTATES, BACILLUS amyloliquefaciens
Abstract

Background:Previously, a safe strain, Bacillus amyloliquefaciens B10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD). However, B. amyloliquefaciens fermentation yields large quantities of acetoin, lactate and succinate as by-products, and the 2,3-BD yield remains prohibitively low for commercial production. Methodology/Principal Findings:In the 2,3-butanediol metabolic pathway, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of 3-phosphate glyceraldehyde to 1,3-bisphosphoglycerate, with concomitant reduction of NAD+ to NADH. In the same pathway, 2,3-BD dehydrogenase (BDH) catalyzes the conversion of acetoin to 2,3-BD with concomitant oxidation of NADH to NAD+. In this study, to improve 2,3-BD production, we first over-produced NAD+-dependent GAPDH and NADH-dependent BDH in B. amyloliquefaciens. Excess GAPDH reduced the fermentation time, increased the 2,3-BD yield by 12.7%, and decreased the acetoin titer by 44.3%. However, the process also enhanced lactate and succinate production. Excess BDH increased the 2,3-BD yield by 16.6% while decreasing acetoin, lactate and succinate production, but prolonged the fermentation time. When BDH and GAPDH were co-overproduced in B. amyloliquefaciens, the fermentation time was reduced. Furthermore, in the NADH-dependent pathways, the molar yield of 2,3-BD was increased by 22.7%, while those of acetoin, lactate and succinate were reduced by 80.8%, 33.3% and 39.5%, relative to the parent strain. In fed-batch fermentations, the 2,3-BD concentration was maximized at 132.9 g/l after 45 h, with a productivity of 2.95 g/l·h. Conclusions/Significance:Co-overexpression of bdh and gapA genes proved an effective method for enhancing 2,3-BD production and inhibiting the accumulation of unwanted by-products (acetoin, lactate and succinate). To our knowledge, we have attained the highest 2,3-BD fermentation yield thus far reported for safe microorganisms. [ABSTRACT FROM AUTHOR]

Published
2013
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