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Your search keyword '"Yeh, Heidi"' showing total 10 results
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10 results on '"Yeh, Heidi"'

1. Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol

Author

Pendexter, Casie A., primary, Haque, Omar, additional, Mojoudi, Mohammadreza, additional, Maggipinto, Sarah, additional, Goutard, Marion, additional, Baicu, Simona, additional, Lellouch, Alexandre G., additional, Markmann, James F., additional, Brandacher, Gerald, additional, Yeh, Heidi, additional, Tessier, Shannon N., additional, Cetrulo, Curtis, additional, and Uygun, Korkut, additional

Published
2023
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2. Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

Author

de Vries, Reinier J., primary, Cronin, Stephanie E. J., additional, Romfh, Padraic, additional, Pendexter, Casie A., additional, Jain, Rohil, additional, Wilks, Benjamin T., additional, Raigani, Siavash, additional, van Gulik, Thomas M., additional, Chen, Peili, additional, Yeh, Heidi, additional, Uygun, Korkut, additional, and Tessier, Shannon N., additional

Published
2021
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4. Metabolic and lipidomic profiling of steatotic human livers during ex situ normothermic machine perfusion guides resuscitation strategies

Author

Raigani, Siavash, primary, Karimian, Negin, additional, Huang, Viola, additional, Zhang, Anna M., additional, Beijert, Irene, additional, Geerts, Sharon, additional, Nagpal, Sonal, additional, Hafiz, Ehab O. A., additional, Fontan, Fermin M., additional, Aburawi, Mohamed M., additional, Mahboub, Paria, additional, Markmann, James F., additional, Porte, Robert J., additional, Uygun, Korkut, additional, Yarmush, Martin, additional, and Yeh, Heidi, additional

Published
2020
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6. Xenogeneic Heterotopic Auxiliary Liver transplantation (XHALT) promotes native liver regeneration in a Post-Hepatectomy Liver failure model

Author

Navarro-Alvarez, Nalu, primary, Machaidze, Zurab, additional, Schuetz, Christian, additional, Zhu, Alexander, additional, Liu, Wei-hui, additional, Shah, Jigesh A., additional, Vagefi, Parsia A., additional, Elias, Nahel, additional, Buhler, Leo, additional, Sachs, David H., additional, Markmann, James F., additional, and Yeh, Heidi, additional

Published
2018
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9. Mechanisms of Xenogeneic Baboon Platelet Aggregation and Phagocytosis by Porcine Liver Sinusoidal Endothelial Cells

Author

Peng, Qiang, primary, Yeh, Heidi, additional, Wei, Lingling, additional, Enjyoj, Keiichi, additional, Machaidze, Zurab, additional, Csizmad, Eva, additional, Schuetz, Christian, additional, Lee, Kang Mi, additional, Deng, Shaoping, additional, Robson, Simon C., additional, Markmann, James, additional, and Buhler, Leo, additional

Published
2012
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10. Mechanisms of Xenogeneic Baboon Platelet Aggregation and Phagocytosis by Porcine Liver Sinusoidal Endothelial Cells.

Author

Qiang Peng, Yeh, Heidi, Lingling Wei, Enjyoj, Keiichi, Machaidze, Zurab, Csizmad, Eva, Schuetz, Christian, Kang Mi Lee, Shaoping Deng, Robson, Simon C., Markmann, James, Buhler, Leo, and Lenting, Peter J.

Subjects
*BABOONS, *LIVER transplantation, *BLOOD platelets, *THROMBOCYTOPENIA, *HEMORRHAGE, *ENDOTHELIAL cells
Abstract

Background: Baboons receiving xenogeneic livers from wild type and transgenic pigs survive less than 10 days. One of the major issues is the early development of profound thrombocytopenia that results in fatal hemorrhage. Histological examination of xenotransplanted livers has shown baboon platelet activation, phagocytosis and sequestration within the sinusoids. In order to study the mechanisms of platelet consumption in liver xenotransplantation, we have developed an in vitro system to examine the interaction between pig endothelial cells with baboon platelets and to thereby identify molecular mechanisms and therapies. Methods: Fresh pig hepatocytes, liver sinusoidal and aortic endothelial cells were isolated by collagenase digestion of livers and processing of aortae from GTKO and Gal+ MGH-miniature swine. These primary cell cultures were then tested for the differential ability to induce baboon or pig platelet aggregation. Phagocytosis was evaluated by direct observation of CFSE labeled-platelets, which are incubated with endothelial cells under confocal light microscopy. Aurintricarboxylic acid (GpIb antagonist blocking interactions with von Willebrand factor/vWF), eptifibatide (Gp IIb/IIIa antagonist), and anti-Mac-1 Ab (anti-αMβ2 integrin Ab) were tested for the ability to inhibit phagocytosis. Results: None of the pig cells induced aggregation or phagocytosis of porcine platelets. However, pig hepatocytes, liver sinusoidal and aortic endothelial cells (GTKO and Gal+) all induced moderate aggregation of baboon platelets. Importantly, pig liver sinusoidal endothelial cells efficiently phagocytosed baboon platelets, while pig aortic endothelial cells and hepatocytes had minimal effects on platelet numbers. Anti-MAC-1 Ab, aurintricarboxylic acid or eptifibatide, significantly decreased baboon platelet phagocytosis by pig liver endothelial cells (P<0.01). Conclusions: Although pig hepatocytes and aortic endothelial cells directly caused aggregation of baboon platelets, only pig liver endothelial cells efficiently phagocytosed baboon platelets. Blocking vWF and integrin adhesion pathways prevented both aggregation and phagocytosis. [ABSTRACT FROM AUTHOR]

Published
2012
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