26 results on '"Yuan, Qiang"'
Search Results
2. Serum creatinine in predicting mortality after paraquat poisoning: A systematic review and meta-analysis
- Author
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Wei Huang, Zheng Zhang, and Yuan-Qiang Lu
- Subjects
Medicine ,Science - Abstract
Although the prognostic value of blood creatinine levels in patients with paraquat (PQ) poisoning has been studied for a long time, the results are still controversial. Therefore, we performed the first meta-analysis to comprehensively assess the value of blood creatinine in predicting the prognosis of patients with PQ poisoning. We searched PubMed, EMBase, Web of Science, ScienceDirect, Cochrane Library, China National Knowledge Infrastructure, China Science and Technology Journal Database, and China Online Journals to identify all relevant papers published up to June 2022. Data were extracted for pooled analysis, heterogeneity testing, sensitivity analysis, publication bias analysis, and subgroup analysis. Ultimately, 10 studies involving 862 patients were included. The I2 of diagnostic odds ratio (DOR), sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio of this study were all greater than 50%, which showed the existence of heterogeneity in this study, and a random effects model was used for the combination of the above five effect sizes. Pooled analysis showed a high predictive value of blood creatinine for prognosis of PQ poisoning [pooled DOR:22.92, 95% confidence interval (CI):15.62–33.65, P < 0.001]. The combined sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio were 86% (95% CI: 0.79–0.91), 78% (95% CI: 0.69–0.86), 4.01 (95% CI: 2.81–5.71), and 0.17 (95% CI: 0.12–0.25), respectively. Deeks publication bias test revealed there was publication bias. Sensitivity analysis showed no significant differences in the estimates of impact. Serum creatinine is an effective predictor of mortality in patients with PQ poisoning.
- Published
- 2023
3. Retraction: TGF-β1 Regulation of Estrogen Production in Mature Rat Leydig Cells
- Author
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Liu, Man-Li, primary, Wang, Huan, additional, Wang, Zong-Ren, additional, Zhang, Yu-Fen, additional, Chen, Yan-Qiu, additional, Zhu, Fang-Hong, additional, Zhang, Yuan-Qiang, additional, Ma, Jing, additional, and Li, Zhen, additional
- Published
- 2024
- Full Text
- View/download PDF
4. Serum creatinine in predicting mortality after paraquat poisoning: A systematic review and meta-analysis
- Author
-
Huang, Wei, primary, Zhang, Zheng, additional, and Lu, Yuan-Qiang, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Abnormal Accumulation of Collagen Type I Due to the Loss of Discoidin Domain Receptor 2 (Ddr2) Promotes Testicular Interstitial Dysfunction.
- Author
-
Chu-chao Zhu, Bin Tang, Jin Su, Hu Zhao, Xin Bu, Zhen Li, Jie Zhao, Wei-dong Gong, Zhi-qun Wu, Li-bo Yao, Wei Li, and Yuan-qiang Zhang
- Subjects
Medicine ,Science - Abstract
Loss of functional allele for discoidin domain receptor 2 (Ddr2) results in impaired Leydig cell response to luteinizing hormone (LH), low testosterone production and arrested spermatogenesis in older male Ddr2slie/slie mice. However, the underlying mechanism responsible for this phenotype remains unknown. Herein, we reported for the first time that the deregulated expression of Ddr2 cognate ligand, namely collagen type I (COL1), may account for the disruption of the testicular steroidogenesis in Ddr2slie/slie mutant testes.Expression of Ddr2 increased gradually along postnatal development, whereas COL1 expression became negligible from adulthood onwards. In Ddr2slie/slie mutant testis, however, in contrast to the undetectable staining of Ddr2, COL1 expression was constantly detected, with the highest values detected during adulthood. In the experimental vasectomy model, Ddr2slie/slie mutant mice exhibited an early androgen deficiency than wild-type mice, along with the accumulation of fibrotic tissue in the interstitium. Functionally, ablation of endogenous Ddr2 resulted in a significant decrease of testosterone (T) level in TM3 cells in the presence of higher concentration of COL1 treatment. Conversely, overexpression of Ddr2 could help TM3 cells to maintain a normal testicular steroidogenesis even in the presence of high concentration of COL1. Additionally, attenuated expression of Ddr2 correlates to the deregulated level of serum T levels in human pathological testes.Abnormal accumulation of interstitial COL1 may be responsible for the steroidogenic dysfunction in Ddr2slie/slie mutant testes.
- Published
- 2015
- Full Text
- View/download PDF
6. Conserved dopamine neurotrophic factor-transduced mesenchymal stem cells promote axon regeneration and functional recovery of injured sciatic nerve.
- Author
-
Yi Liu, Lin Nie, Hua Zhao, Wen Zhang, Yuan-Qiang Zhang, Shuai-Shuai Wang, and Lei Cheng
- Subjects
Medicine ,Science - Abstract
Peripheral nerve injury (PNI) is a common disease that often results in axonal degeneration and the loss of neurons, ultimately leading to limited nerve regeneration and severe functional impairment. Currently, there are no effective treatments for PNI. In the present study, we transduced conserved dopamine neurotrophic factor (CDNF) into mesenchymal stem cells (MSCs) in collagen tubes to investigate their regenerative effects on rat peripheral nerves in an in vivo transection model. Scanning electron microscopy of the collagen tubes demonstrated their ability to be resorbed in vivo. We observed notable overexpression of the CDNF protein in the distal sciatic nerve after application of CDNF-MSCs. Quantitative analysis of neurofilament 200 (NF200) and S100 immunohistochemistry showed significant enhancement of axonal and Schwann cell regeneration in the group receiving CDNF-MSCs (CDNF-MSCs group) compared with the control groups. Myelination thickness, axon diameter and the axon-to fiber diameter ratio (G-ratio) were significantly higher in the CDNF-MSCs group at 8 and 12 weeks after nerve transection surgery. After surgery, the sciatic functional index, target muscle weight, wet weight ratio of gastrocnemius muscle and horseradish peroxidase (HRP) tracing demonstrated functional recovery. Light and electron microscopy confirmed successful regeneration of the sciatic nerve. The greater numbers of HRP-labeled neuron cell bodies and increased sciatic nerve index values (SFI) in the CDNF-MSCs group suggest that CDNF exerts neuroprotective effects in vivo. We also observed higher target muscle weights and a significant improvement in muscle atrophism in the CDNF-MSCs group. Collectively, these findings indicate that CDNF gene therapy delivered by MSCs is capable of promoting nerve regeneration and functional recovery, likely because of the significant neuroprotective and neurotrophic effects of CDNF and the superior environment offered by MSCs and collagen tubes.
- Published
- 2014
- Full Text
- View/download PDF
7. TGF-β1 regulation of estrogen production in mature rat Leydig cells.
- Author
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Man-Li Liu, Huan Wang, Zong-Ren Wang, Yu-Fen Zhang, Yan-Qiu Chen, Fang-Hong Zhu, Yuan-Qiang Zhang, Jing Ma, and Zhen Li
- Subjects
Medicine ,Science - Abstract
BACKGROUND: Besides androgens, estrogens produced in Leydig cells are also crucial for mammalian germ cell differentiation. Transforming growth factor-β1 (TGF-β1) is now known to have multiple effects on regulation of Leydig cell function. The objective of the present study is to determine whether TGF-β1 regulates estradiol (E2) synthesis in adult rat Leydig cells and then to assess the impact of TGF-β1 on Cx43-based gap junctional intercellular communication (GJIC) between Leydig cells. METHODOLOGY/PRINCIPAL FINDINGS: Primary cultured Leydig cells were incubated in the presence of recombinant TGF-β1 and the production of E2 as well as testosterone (T) were measured by RIA. The activity of P450arom was addressed by the tritiated water release assay and the expression of Cyp19 gene was evaluated by Western blotting and real time RT-PCR. The expression of Cx43 and GJIC were investigated with immunofluorescence and fluorescence recovery after photo-bleaching (FRAP), respectively. Results from this study show that TGF-β1 down-regulates the level of E2 secretion and the activity of P450arom in a dose-dependent manner in adult Leydig cells. In addition, the expression of Cx43 and GJIC was closely related to the regulation of E2 and TGF-β1, and E2 treatment in turn restored the inhibition of TGF-β1 on GJIC. CONCLUSIONS: Our results indicate, for the first time in adult rat Leydig cells, that TGF-β1 suppresses P450arom activity, as well as the expression of the Cyp19 gene, and that depression of E2 secretion leads to down-regulation of Cx43-based GJIC between Leydig cells.
- Published
- 2013
- Full Text
- View/download PDF
8. Genetic variation in the EGFR gene and the risk of glioma in a Chinese Han population.
- Author
-
Wu-Gang Hou, Wen-Bo Ai, Xiao-Guang Bai, Hai-Long Dong, Zhen Li, Yuan-Qiang Zhang, and Li-Ze Xiong
- Subjects
Medicine ,Science - Abstract
Previous studies have shown that regulation of the epidermal growth factor gene (EGFR) pathway plays a role in glioma progression. Certain genotypes of the EGFR gene may be related to increased glioblastoma risk, indicating that germ line EGFR polymorphisms may have implications in carcinogenesis. To examine whether and how variants in the EGFR gene contribute to glioma susceptibility, we evaluated nine tagging single-nucleotide polymorphisms (tSNPs) of the EGFR gene in a case-control study from Xi'an city of China (301 cases, 302 controls). EGFR SNP associations analyses were performed using SPSS 16.0 statistical packages, PLINK software, Haploview software package (version 4.2) and SHEsis software platform. We identified two susceptibility tSNPs in the EGFR gene that were potentially associated with an increased risk of glioma (rs730437, p = 0.016; OR: 1.32; 95%CI: 1.05-1.66 and rs1468727, p = 0.008; OR: 1.31; 95%CI: 1.04-1.65). However, after a strict Bonferroni correction analysis was applied, the significance level of the association between EGFR tSNPs and risk of glioma was attenuated. We observed a protective effect of haplotype "AATT" of the EGFR gene, which was associated with a 29% reduction in the risk of developing glioma, while haplotype "CGTC" increased the risk of developing glioma by 36%. Our results, combined with previous studies, suggested an association between the EGFR gene and glioma development.
- Published
- 2012
- Full Text
- View/download PDF
9. Transient protection from heat-stress induced apoptotic stimulation by metastasis-associated protein 1 in pachytene spermatocytes.
- Author
-
Wei Li, Zhi-qun Wu, Jie Zhao, Sheng-jie Guo, Zhen Li, Xiao Feng, Li Ma, Jin-shan Zhang, Xin-ping Liu, and Yuan-qiang Zhang
- Subjects
Medicine ,Science - Abstract
BackgroundDeregulated thermal factors have been frequently implicated in the pathogenesis of male infertility, but the molecular basis through which certain responses are directed remain largely unknown. We previously reported that overexpression of exogenous Metastasis-associated protein 1 (MTA1) protects spermatogenic tumor cells GC-2spd (ts) against heat-induced apoptosis. To further dissect the underlying mechanism, we addressed here the fine coordination between MTA1 and p53 in pachytene spermatocytes upon hyperthermal stimulation.Methodology/principal findingsHigh level of MTA1 expression sustained for 1.5 h in primary spermatocytes after heat stress before a notable decrease was detected conversely correlated to the gradual increase of acetylation status of p53 and of p21 level. Knockdown of the endogenous MTA1 in GC-2spd (ts) elevated the acetylation of p53 by diminishing the recruitment of HDAC2 and thereafter led to a dramatic increase of apoptosis after heat treatment. Consistent with this, in vivo interference of MTA1 expression in the testes of C57BL/6 mice also urged an impairment of the differentiation of spermatocytes and a disruption of Sertoli cell function due to the elevated apoptotic rate after heat stress. Finally, attenuated expression of MTA1 of pachytene spermatocytes was observed in arrested testes (at the round spermatid level) of human varicocele patients.ConclusionsThese data underscore a transient protective effect of this histone modifier in primary spermatocytes against heat-stress, which may operate as a negative coregulator of p53 in maintenance of apoptotic balance during early phase after hyperthermal stress.
- Published
- 2011
- Full Text
- View/download PDF
10. Localization and androgen regulation of metastasis-associated protein 1 in mouse epididymis.
- Author
-
Li Ma, Wei Li, Hua-Ping Zhu, Zhen Li, Zhi-Jian Sun, Xin-Ping Liu, Jie Zhao, Jin-Shan Zhang, and Yuan-Qiang Zhang
- Subjects
Medicine ,Science - Abstract
BACKGROUND: Metastasis-associated protein 1 (MTA1), the founding member of the MTA family of genes, can modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of cancer cells, MTA1 can also regulate crucial cellular pathways by modifying the acetylation status. We have previously reported the presence of MTA1/MTA1 in human and mouse testes, providing the evidence for its involvement in the regulation of testicular function during murine spermatogenesis. The objective of present study was to further assess the localization of MTA1 in mouse epididymis on both transcriptional and translational level, and then to explore whether MTA1 expression is regulated by androgens and postnatal epididymal development. METHODOLOGY/PRINCIPAL FINDINGS: Mice were deprived of circulating androgen by bilaterally castration and were then supplemented with exogenous testosterone propionate for one week. MTA1 was immunolocalized in the epithelium of the entire epididymis with the maximal expression in the nuclei of principal cells and of clear cells in proximal region. Its expression decreased gradually after castration, whereas testosterone treatment could restore the expression, indicating that the expression of this gene is dependent on androgen. During postnatal development, the protein expression in the epididymis began to appear from day 7 to day 14, increased dramatically from postnatal day 28, and peaked at adulthood onwards, coinciding with both the well differentiated status of epididymis and the mature levels of circulating androgens. This region- and cell-specific pattern was also conservative in normal human epididymis. CONCLUSIONS: Our data suggest that the expression of MTA1 protein could be regulated by androgen pathway and its expression level is closely associated with the postnatal development of the epididymis, giving rise to the possibility that this gene plays a potential role in sperm maturation and fertility.
- Published
- 2010
- Full Text
- View/download PDF
11. L-Type Calcium Channel Inhibition Contributes to the Proarrhythmic Effects of Aconitine in Human Cardiomyocytes
- Author
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Wu, Jianjun, primary, Wang, Xiangchong, additional, Chung, Ying Ying, additional, Koh, Cai Hong, additional, Liu, Zhenfeng, additional, Guo, Huicai, additional, Yuan, Qiang, additional, Wang, Chuan, additional, Su, Suwen, additional, and Wei, Heming, additional
- Published
- 2017
- Full Text
- View/download PDF
12. Abnormal Accumulation of Collagen Type I Due to the Loss of Discoidin Domain Receptor 2 (Ddr2) Promotes Testicular Interstitial Dysfunction
- Author
-
Zhi-qun Wu, Yuan-Qiang Zhang, Wei-dong Gong, Bin Tang, Zhen Li, Xin Bu, Jin Su, Jie Zhao, Wei Li, Hu Zhao, Libo Yao, and Chuchao Zhu
- Subjects
Male ,medicine.medical_specialty ,Radioimmunoassay ,lcsh:Medicine ,Biology ,Collagen Type I ,Cell Line ,Sertoli cell-only syndrome ,Mice ,Fibrosis ,Internal medicine ,Testis ,medicine ,Animals ,Humans ,Testosterone ,RNA, Small Interfering ,Receptor ,lcsh:Science ,Discoidin Domain Receptors ,Mice, Knockout ,Multidisciplinary ,Leydig cell ,Sertoli Cell-Only Syndrome ,lcsh:R ,Leydig Cells ,Receptor Protein-Tyrosine Kinases ,Oligospermia ,medicine.disease ,Immunohistochemistry ,Mice, Inbred C57BL ,Disease Models, Animal ,medicine.anatomical_structure ,Endocrinology ,Microscopy, Fluorescence ,Receptors, Mitogen ,RNA Interference ,lcsh:Q ,Luteinizing hormone ,Spermatogenesis ,Discoidin domain ,Research Article - Abstract
Background Loss of functional allele for discoidin domain receptor 2 (Ddr2) results in impaired Leydig cell response to luteinizing hormone (LH), low testosterone production and arrested spermatogenesis in older male Ddr2slie/slie mice. However, the underlying mechanism responsible for this phenotype remains unknown. Herein, we reported for the first time that the deregulated expression of Ddr2 cognate ligand, namely collagen type I (COL1), may account for the disruption of the testicular steroidogenesis in Ddr2slie/slie mutant testes. Methodology/Principal Findings Expression of Ddr2 increased gradually along postnatal development, whereas COL1 expression became negligible from adulthood onwards. In Ddr2slie/slie mutant testis, however, in contrast to the undetectable staining of Ddr2, COL1 expression was constantly detected, with the highest values detected during adulthood. In the experimental vasectomy model, Ddr2slie/slie mutant mice exhibited an early androgen deficiency than wild-type mice, along with the accumulation of fibrotic tissue in the interstitium. Functionally, ablation of endogenous Ddr2 resulted in a significant decrease of testosterone (T) level in TM3 cells in the presence of higher concentration of COL1 treatment. Conversely, overexpression of Ddr2 could help TM3 cells to maintain a normal testicular steroidogenesis even in the presence of high concentration of COL1. Additionally, attenuated expression of Ddr2 correlates to the deregulated level of serum T levels in human pathological testes. Conclusions Abnormal accumulation of interstitial COL1 may be responsible for the steroidogenic dysfunction in Ddr2slie/slie mutant testes.
- Published
- 2015
13. TGF-β1 regulation of estrogen production in mature rat Leydig cells
- Author
-
Zhen Li, Huan Wang, Yuan-Qiang Zhang, Yan-Qiu Chen, Yu-Fen Zhang, Man-Li Liu, Jing Ma, Fang-Hong Zhu, and Zongren Wang
- Subjects
Male ,Anatomy and Physiology ,lcsh:Medicine ,Polymerase Chain Reaction ,Biochemistry ,Rats, Sprague-Dawley ,Gene expression ,Molecular Cell Biology ,Testosterone ,lcsh:Science ,Cells, Cultured ,Multidisciplinary ,Leydig cell ,Gap Junctions ,Leydig Cells ,Cell Differentiation ,Animal Models ,Cell biology ,medicine.anatomical_structure ,Medicine ,Cellular Types ,Germ cell ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,medicine.medical_specialty ,Cell signaling ,endocrine system ,medicine.drug_class ,Blotting, Western ,Radioimmunoassay ,Biology ,Transforming Growth Factor beta1 ,Model Organisms ,Internal medicine ,medicine ,Animals ,Secretion ,lcsh:R ,Reproductive System ,Estrogens ,Hormones ,Rats ,Endocrinology ,Estrogen ,Rat ,lcsh:Q ,Transforming growth factor ,Developmental Biology - Abstract
Background Besides androgens, estrogens produced in Leydig cells are also crucial for mammalian germ cell differentiation. Transforming growth factor-β1 (TGF-β1) is now known to have multiple effects on regulation of Leydig cell function. The objective of the present study is to determine whether TGF-β1 regulates estradiol (E2) synthesis in adult rat Leydig cells and then to assess the impact of TGF-β1 on Cx43-based gap junctional intercellular communication (GJIC) between Leydig cells. Methodology/Principal Findings Primary cultured Leydig cells were incubated in the presence of recombinant TGF-β1 and the production of E2 as well as testosterone (T) were measured by RIA. The activity of P450arom was addressed by the tritiated water release assay and the expression of Cyp19 gene was evaluated by Western blotting and real time RT-PCR. The expression of Cx43 and GJIC were investigated with immunofluorescence and fluorescence recovery after photo-bleaching (FRAP), respectively. Results from this study show that TGF-β1 down-regulates the level of E2 secretion and the activity of P450arom in a dose-dependent manner in adult Leydig cells. In addition, the expression of Cx43 and GJIC was closely related to the regulation of E2 and TGF-β1, and E2 treatment in turn restored the inhibition of TGF-β1 on GJIC. Conclusions Our results indicate, for the first time in adult rat Leydig cells, that TGF-β1 suppresses P450arom activity, as well as the expression of the Cyp19 gene, and that depression of E2 secretion leads to down-regulation of Cx43-based GJIC between Leydig cells.
- Published
- 2012
14. Genetic variation in the EGFR gene and the risk of glioma in a Chinese Han population
- Author
-
Yuan-Qiang Zhang, Xiaoguang Bai, Wen-Bo Ai, Lize Xiong, Wugang Hou, Hailong Dong, and Zhen Li
- Subjects
Male ,Oncology ,Heredity ,lcsh:Medicine ,Bioinformatics ,Gene Frequency ,Polymorphism (computer science) ,Epidermal growth factor ,lcsh:Science ,Neurological Tumors ,Multidisciplinary ,Cancer Risk Factors ,Glioma ,Middle Aged ,ErbB Receptors ,Medicine ,Female ,Research Article ,Adult ,Risk ,China ,medicine.medical_specialty ,Genotype ,Haploview ,Genotypes ,Genetic Causes of Cancer ,Biology ,Polymorphism, Single Nucleotide ,Young Adult ,Asian People ,Internal medicine ,Genetics ,Cancer Genetics ,medicine ,Humans ,SNP ,Genetic Predisposition to Disease ,Allele frequency ,Haplotype ,lcsh:R ,Case-control study ,Cancers and Neoplasms ,medicine.disease ,Case-Control Studies ,Genetic Polymorphism ,lcsh:Q ,Population Genetics - Abstract
Previous studies have shown that regulation of the epidermal growth factor gene (EGFR) pathway plays a role in glioma progression. Certain genotypes of the EGFR gene may be related to increased glioblastoma risk, indicating that germ line EGFR polymorphisms may have implications in carcinogenesis. To examine whether and how variants in the EGFR gene contribute to glioma susceptibility, we evaluated nine tagging single-nucleotide polymorphisms (tSNPs) of the EGFR gene in a case-control study from Xi'an city of China (301 cases, 302 controls). EGFR SNP associations analyses were performed using SPSS 16.0 statistical packages, PLINK software, Haploview software package (version 4.2) and SHEsis software platform. We identified two susceptibility tSNPs in the EGFR gene that were potentially associated with an increased risk of glioma (rs730437, p = 0.016; OR: 1.32; 95%CI: 1.05-1.66 and rs1468727, p = 0.008; OR: 1.31; 95%CI: 1.04-1.65). However, after a strict Bonferroni correction analysis was applied, the significance level of the association between EGFR tSNPs and risk of glioma was attenuated. We observed a protective effect of haplotype "AATT" of the EGFR gene, which was associated with a 29% reduction in the risk of developing glioma, while haplotype "CGTC" increased the risk of developing glioma by 36%. Our results, combined with previous studies, suggested an association between the EGFR gene and glioma development.
- Published
- 2012
15. Transient protection from heat-stress induced apoptotic stimulation by metastasis-associated protein 1 in pachytene spermatocytes
- Author
-
Zhen Li, Li Ma, Xinping Liu, Xiao Feng, Zhi-qun Wu, Yuan-Qiang Zhang, Wei Li, Sheng-jie Guo, Jinshan Zhang, and Jie Zhao
- Subjects
Male ,Anatomy and Physiology ,Histone Deacetylase 2 ,Stimulation ,Apoptosis ,Metastasis ,Male infertility ,Pathogenesis ,Mice ,Reproductive Physiology ,Spermatocytes ,Molecular Cell Biology ,Testis ,Signaling in Cellular Processes ,Apoptotic Signaling Cascade ,Apoptotic Signaling ,Multidisciplinary ,Cell Death ,Temperature ,Acetylation ,Immunohistochemistry ,Signaling Cascades ,Heat stress ,Cell biology ,Up-Regulation ,Medicine ,Cellular Types ,Research Article ,Signal Transduction ,Hyperthermia ,Adult ,Urology ,Science ,Tumor cells ,Biology ,Models, Biological ,Histone Deacetylases ,medicine ,Animals ,Humans ,Spermatogenesis ,Infertility, Male ,Reproductive System ,Hyperthermia, Induced ,medicine.disease ,Molecular biology ,Repressor Proteins ,Tetraploidy ,Germ Cells ,Cytoprotection ,Infertility ,Trans-Activators ,Pachytene Stage ,Tumor Suppressor Protein p53 ,Heat-Shock Response ,Transcription Factors - Abstract
BackgroundDeregulated thermal factors have been frequently implicated in the pathogenesis of male infertility, but the molecular basis through which certain responses are directed remain largely unknown. We previously reported that overexpression of exogenous Metastasis-associated protein 1 (MTA1) protects spermatogenic tumor cells GC-2spd (ts) against heat-induced apoptosis. To further dissect the underlying mechanism, we addressed here the fine coordination between MTA1 and p53 in pachytene spermatocytes upon hyperthermal stimulation.Methodology/principal findingsHigh level of MTA1 expression sustained for 1.5 h in primary spermatocytes after heat stress before a notable decrease was detected conversely correlated to the gradual increase of acetylation status of p53 and of p21 level. Knockdown of the endogenous MTA1 in GC-2spd (ts) elevated the acetylation of p53 by diminishing the recruitment of HDAC2 and thereafter led to a dramatic increase of apoptosis after heat treatment. Consistent with this, in vivo interference of MTA1 expression in the testes of C57BL/6 mice also urged an impairment of the differentiation of spermatocytes and a disruption of Sertoli cell function due to the elevated apoptotic rate after heat stress. Finally, attenuated expression of MTA1 of pachytene spermatocytes was observed in arrested testes (at the round spermatid level) of human varicocele patients.ConclusionsThese data underscore a transient protective effect of this histone modifier in primary spermatocytes against heat-stress, which may operate as a negative coregulator of p53 in maintenance of apoptotic balance during early phase after hyperthermal stress.
- Published
- 2011
16. Abnormal Accumulation of Collagen Type I Due to the Loss of Discoidin Domain Receptor 2 (Ddr2) Promotes Testicular Interstitial Dysfunction
- Author
-
Zhu, Chu-chao, primary, Tang, Bin, additional, Su, Jin, additional, Zhao, Hu, additional, Bu, Xin, additional, Li, Zhen, additional, Zhao, Jie, additional, Gong, Wei-dong, additional, Wu, Zhi-qun, additional, Yao, Li-bo, additional, Li, Wei, additional, and Zhang, Yuan-qiang, additional
- Published
- 2015
- Full Text
- View/download PDF
17. Localization and androgen regulation of metastasis-associated protein 1 in mouse epididymis
- Author
-
Yuan-Qiang Zhang, Xinping Liu, Zhi-jian Sun, Li Ma, Wei Li, Zhen Li, Jie Zhao, Hua-Ping Zhu, and Jinshan Zhang
- Subjects
Male ,Time Factors ,Anatomy and Physiology ,Mouse ,lcsh:Medicine ,chemistry.chemical_compound ,Mice ,Reproductive Physiology ,Gene expression ,Molecular Cell Biology ,Testosterone ,Orchiectomy ,lcsh:Science ,Epididymis ,Mice, Inbred BALB C ,Multidisciplinary ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Regulation, Developmental ,Animal Models ,Immunohistochemistry ,medicine.anatomical_structure ,Androgens ,Medicine ,Research Article ,medicine.medical_specialty ,medicine.drug_class ,Blotting, Western ,Endocrine System ,Biology ,Chromatin remodeling ,Model Organisms ,Internal medicine ,medicine ,Animals ,Humans ,Reproductive Endocrinology ,Endocrine Physiology ,lcsh:R ,Reproductive System ,Molecular Development ,Androgen ,Repressor Proteins ,Endocrinology ,Castration ,chemistry ,Microscopy, Fluorescence ,Cancer cell ,Trans-Activators ,lcsh:Q ,Spermatogenesis ,Transcription Factors ,Developmental Biology - Abstract
Background Metastasis-associated protein 1 (MTA1), the founding member of the MTA family of genes, can modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of cancer cells, MTA1 can also regulate crucial cellular pathways by modifying the acetylation status. We have previously reported the presence of MTA1/MTA1 in human and mouse testes, providing the evidence for its involvement in the regulation of testicular function during murine spermatogenesis. The objective of present study was to further assess the localization of MTA1 in mouse epididymis on both transcriptional and translational level, and then to explore whether MTA1 expression is regulated by androgens and postnatal epididymal development. Methodology/Principal Findings Mice were deprived of circulating androgen by bilaterally castration and were then supplemented with exogenous testosterone propionate for one week. MTA1 was immunolocalized in the epithelium of the entire epididymis with the maximal expression in the nuclei of principal cells and of clear cells in proximal region. Its expression decreased gradually after castration, whereas testosterone treatment could restore the expression, indicating that the expression of this gene is dependent on androgen. During postnatal development, the protein expression in the epididymis began to appear from day 7 to day 14, increased dramatically from postnatal day 28, and peaked at adulthood onwards, coinciding with both the well differentiated status of epididymis and the mature levels of circulating androgens. This region- and cell-specific pattern was also conservative in normal human epididymis. Conclusions Our data suggest that the expression of MTA1 protein could be regulated by androgen pathway and its expression level is closely associated with the postnatal development of the epididymis, giving rise to the possibility that this gene plays a potential role in sperm maturation and fertility.
- Published
- 2010
18. Conserved Dopamine Neurotrophic Factor-Transduced Mesenchymal Stem Cells Promote Axon Regeneration and Functional Recovery of Injured Sciatic Nerve
- Author
-
Liu, Yi, primary, Nie, Lin, additional, Zhao, Hua, additional, Zhang, Wen, additional, Zhang, Yuan-Qiang, additional, Wang, Shuai-Shuai, additional, and Cheng, Lei, additional
- Published
- 2014
- Full Text
- View/download PDF
19. The Prognostic Value of Serum Neuron-Specific Enolase in Traumatic Brain Injury: Systematic Review and Meta-Analysis
- Author
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Cheng, Feng, primary, Yuan, Qiang, additional, Yang, Jian, additional, Wang, Wenming, additional, and Liu, Hua, additional
- Published
- 2014
- Full Text
- View/download PDF
20. Inhibition of Phosphatase and Tensin Homolog Deleted on Chromosome 10 Decreases Rat Cortical Neuron Injury and Blood-Brain Barrier Permeability, and Improves Neurological Functional Recovery in Traumatic Brain Injury Model
- Author
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Ding, Jun, primary, Guo, Jianyi, additional, Yuan, Qiang, additional, Yuan, Fang, additional, Chen, Hao, additional, and Tian, Hengli, additional
- Published
- 2013
- Full Text
- View/download PDF
21. Impact of Thoracic Injury on Traumatic Brain Injury Outcome
- Author
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Dai, Dawei, primary, Yuan, Qiang, additional, Sun, Yinfeng, additional, Yuan, Fang, additional, Su, Zuopeng, additional, Ding, Jun, additional, and Tian, Hengli, additional
- Published
- 2013
- Full Text
- View/download PDF
22. TGF-β1 Regulation of Estrogen Production in Mature Rat Leydig Cells
- Author
-
Liu, Man-Li, primary, Wang, Huan, additional, Wang, Zong-Ren, additional, Zhang, Yu-Fen, additional, Chen, Yan-Qiu, additional, Zhu, Fang-Hong, additional, Zhang, Yuan-Qiang, additional, Ma, Jing, additional, and Li, Zhen, additional
- Published
- 2013
- Full Text
- View/download PDF
23. Genetic Variation in the EGFR Gene and the Risk of Glioma in a Chinese Han Population
- Author
-
Hou, Wu-Gang, primary, Ai, Wen-Bo, additional, Bai, Xiao-Guang, additional, Dong, Hai-Long, additional, Li, Zhen, additional, Zhang, Yuan-Qiang, additional, and Xiong, Li-Ze, additional
- Published
- 2012
- Full Text
- View/download PDF
24. Transient Protection from Heat-Stress Induced Apoptotic Stimulation by Metastasis-Associated Protein 1 in Pachytene Spermatocytes
- Author
-
Li, Wei, primary, Wu, Zhi-qun, additional, Zhao, Jie, additional, Guo, Sheng-jie, additional, Li, Zhen, additional, Feng, Xiao, additional, Ma, Li, additional, Zhang, Jin-shan, additional, Liu, Xin-ping, additional, and Zhang, Yuan-qiang, additional
- Published
- 2011
- Full Text
- View/download PDF
25. Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis
- Author
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Ma, Li, primary, Li, Wei, additional, Zhu, Hua-Ping, additional, Li, Zhen, additional, Sun, Zhi-Jian, additional, Liu, Xin-Ping, additional, Zhao, Jie, additional, Zhang, Jin-Shan, additional, and Zhang, Yuan-Qiang, additional
- Published
- 2010
- Full Text
- View/download PDF
26. Functional Analysis of Phosphorylation on Saccharomyces cerevisiae Syntaxin 1 Homologues Sso1p and Sso2p
- Author
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Yuan, Qiang, primary and Jäntti, Jussi, additional
- Published
- 2010
- Full Text
- View/download PDF
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