34 results on '"Yue Hong"'
Search Results
2. Serological testing for SARS-CoV-2 antibodies of employees shows low transmission working in a cancer center
- Author
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Meyerhardt, Jeffrey A., primary, Yue, Hong, additional, Nowak, Radosław P., additional, Brais, Lauren, additional, Ma, Chao, additional, Johnson, Samantha, additional, Harrod, Joanna, additional, Burman, Shourya S. Roy, additional, Hendrickson, Lynn M., additional, Fischinger, Stephanie, additional, Alter, Galit, additional, Hahn, William, additional, Johnson, Bruce E., additional, and Fischer, Eric S., additional
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- 2022
- Full Text
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3. Lactobacillus plantarum improves LPS-induced Caco2 cell line intestinal barrier damage via cyclic AMP-PKA signaling
- Author
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Chen-Xiang Wei, Ju-Hua Wu, Yue-Hong Huang, Xiao-Zhong Wang, and Jian-Ying Li
- Subjects
Intestines ,Lipopolysaccharides ,Multidisciplinary ,Colforsin ,Cyclic AMP ,Animals ,Humans ,Caco-2 Cells ,Lactobacillus plantarum ,Rats - Abstract
Lactobacillus plantarum (LP) has been shown to exhibit protective effects on intestinal barrier function in septic rats, although the regulatory mechanism has not been established. We determined whether LP imparts such protective effects in a lipopolysaccharide (LPS)-induced Caco2 cell monolayer model and whether cAMP-PKA signaling is the underlying mechanism of action. The cyclic adenosine monophosphate (cAMP) agonist, forskolin (FSK), and the protein kinase A (PKA) inhibitor, HT89, were used to study the protective effect of LP on the destruction of the tight junction (TJ) structure of cells treated with LPS and the corresponding changes in cAMP-PKA signaling. Our experimental results demonstrated that LP promoted the expression of TJ proteins between Caco2 cells after LPS treatment, and increased the electrical barrier detection (TEER) between Caco2 cells. Moreover, transmission electron microscopy (TEM) revealed that the TJ structural integrity of cells treated with LPS + LP was improved compared to cells treated with LPS alone. In addition, our findings were consistent between the FSK and LP intervention group, while HT89 inhibited LP influence. Taken together, our results indicate that LP has an improved protective effect on LPS-induced damage to the monolayer membrane barrier function of Caco2 cells and is regulated by the cAMP-PKA pathway.
- Published
- 2021
4. Physiological and genomic features of a novel violacein-producing bacterium isolated from surface seawater
- Author
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Hong Cheng, Yue-Hong Wu, Xiong-Bin Jin, Lin Xu, Xue-Wei Xu, and Chunsheng Wang
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0301 basic medicine ,Indoles ,lcsh:Medicine ,Marine and Aquatic Sciences ,medicine.disease_cause ,Biochemistry ,Database and Informatics Methods ,Pseudoalteromonas ,RNA, Ribosomal, 16S ,lcsh:Science ,Phylogeny ,Data Management ,Multidisciplinary ,Phylogenetic tree ,biology ,Fatty Acids ,Phylogenetic Analysis ,Genomics ,Genomic Databases ,Lipids ,Nucleic acids ,Phylogenetics ,Phenotype ,Ribosomal RNA ,Sequence Analysis ,Research Article ,DNA, Bacterial ,Cell biology ,Computer and Information Sciences ,Cellular structures and organelles ,Bioinformatics ,030106 microbiology ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Glycolipid ,Sea Water ,medicine ,Genetics ,Computer Simulation ,Seawater ,Evolutionary Systematics ,Non-coding RNA ,Taxonomy ,Evolutionary Biology ,Pseudoalteromonas byunsanensis ,lcsh:R ,Ecology and Environmental Sciences ,Biology and Life Sciences ,Computational Biology ,Aquatic Environments ,16S ribosomal RNA ,biology.organism_classification ,Genome Analysis ,Marine Environments ,030104 developmental biology ,Biological Databases ,Earth Sciences ,RNA ,lcsh:Q ,Ribosomes ,Bacteria - Abstract
Strains JW1T and JW3, isolated from surface seawater of the Arabian Sea, were subjected to polyphasic taxonomic analysis. Cells of both strains were Gram-stain-negative, aerobic, and rod-shaped. They formed violet pigment and produced violacein. On the basis of 16S rRNA gene sequence analysis, strains JW1T and JW3 showed high 16S rRNA gene sequence similarity with Pseudoalteromonas byunsanensis JCM12483T (98.2%), P. shioyasakiensis SE3T (97.8%), P. arabiensis JCM 17292T (97.3%), and P. gelatinilytica NH153T (97.1%). The 16S rRNA gene sequence similarity between JW1T and JW3 was 100%. Phylogenetic analyses revealed that both strains fell within the cluster of the genus Pseudoalteromonas and represented an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between JW1T and type strains of the closely related Pseudoalteromonas species were 70.9-83.3% and 20.0-26.4%, respectively. The sole respiratory quinone in both strains is ubiquinone 8 (Q-8). The principal fatty acids are summed feature 3 (C16:1ω7c and/or iso-C15:0 2OH), C18:1ω7c, and C16:0. The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, one unidentified glycolipid, one unidentified aminolipid, and one unidentified phospholipid. The DNA G+C content was 43.3 mol%. Differential phylogenetic distinctiveness, chemotaxonomic differences, and phenotypic properties indicated that strains JW1T and JW3 could be differentiated from the Pseudoalteromonas species with validly published names. Therefore, it is proposed that strains JW1T and JW3 represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas amylolytica sp. nov. (type strain, JW1T = CGMCC 1.15681T = KCTC 52406T = MCCC 1K02162T) is proposed.
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- 2017
5. Revision of series Gravesiana (Adiantum L.) based on morphological characteristics, spores and phylogenetic analyses
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Wan-Wan Zhang, Yue-Hong Yan, Qi-Fei Yi, Xu-Wen Li, Faguo Wang, Lei Duan, Dong-lin Li, Ai-Hua Wang, Xiao-Dong Ma, and Fu-Wu Xing
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0106 biological sciences ,0301 basic medicine ,Spores ,Leaves ,Topography ,lcsh:Medicine ,Plant Science ,01 natural sciences ,Geographical Locations ,Adiantum ,lcsh:Science ,DNA extraction ,Phylogeny ,Data Management ,Multidisciplinary ,biology ,Phylogenetic tree ,Plant Anatomy ,Phylogenetic Analysis ,New Species Reports ,Phylogenetics ,Cliffs ,Leaf Veins ,Research Article ,Abaxial Surface ,Computer and Information Sciences ,China ,Asia ,010603 evolutionary biology ,03 medical and health sciences ,Extraction techniques ,Botany ,Evolutionary Systematics ,Taxonomy ,Evolutionary Biology ,Landforms ,Pinna ,lcsh:R ,Biology and Life Sciences ,Geomorphology ,biology.organism_classification ,Spore ,Research and analysis methods ,030104 developmental biology ,People and Places ,Microscopy, Electron, Scanning ,Earth Sciences ,lcsh:Q - Abstract
Since the adoption of some ambiguous and quantitative characters in Flora Republicae Popularis Sinicae 3(1), species identifications of the series Gravesiana have been in disarray, requiring clarification. Two hundred and fifty-nine individuals from 47 different populations were collected for the estimation of morphological characters and phylogenetic analyses. Spores of 26 populations were observed through scanning electron microscope. Our results were different from those of previous research: (1) six identifiable species, rather than five species observed previously, were confirmed in the series Gravesiana, they are A. gravesii, A. juxtapositum, A. mariesii, A. dentatum, A. longzhouensis and A. obovatum, of which the latter three are newly recognized species. (2) Thirteen characters were measured and estimated through the program Mesquite v. 2.71. The character whether the pinna stalks were 1/3-1/2 times longer than the pinna was used to distinguish A. gravesii and A. lianxianense previously and was found to be unreliable here, whereas such characters as the height of the plant (H), pinna aligned forms (FP), number of pinna (NP), pinna margin (M), number of veins flabellate at base (NV), sori number and shape per pinna (NSS), pinna texture (T), and powder-covered or not on the abaxial surface of the pinna (P) are estimated to be stable and reliable characters useful for identification. Descriptions of new species and their retrieve keys are also listed. (3) Surface ornamentations and spore sizes are helpful for us to distinguish species in series Gravesiana.
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- 2017
6. Physiological and genomic features of a novel violacein-producing bacterium isolated from surface seawater
- Author
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Wu, Yue-Hong, primary, Cheng, Hong, additional, Xu, Lin, additional, Jin, Xiong-Bin, additional, Wang, Chun-Sheng, additional, and Xu, Xue-Wei, additional
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- 2017
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7. Revision of series Gravesiana (Adiantum L.) based on morphological characteristics, spores and phylogenetic analyses
- Author
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Wang, Ai-Hua, primary, Wang, Fa-Guo, additional, Zhang, Wan-Wan, additional, Ma, Xiao-Dong, additional, Li, Xu-Wen, additional, Yi, Qi-Fei, additional, Li, Dong-Lin, additional, Duan, Lei, additional, Yan, Yue-Hong, additional, and Xing, Fu-Wu, additional
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- 2017
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8. Re-establishment of species from synonymies based on DNA barcoding and phylogenetic analysis using Diplopterygium simulans (Gleicheniaceae) as an example
- Author
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Shu, Jiang-Ping, primary, Shang, Hui, additional, Jin, Dongmei, additional, Wei, Hong-Jin, additional, Zhou, Xi-Le, additional, Liu, Hong-Mei, additional, Gu, Yu-Feng, additional, Wang, Ying, additional, Wang, Fa-Guo, additional, Shen, Hui, additional, Zhang, Rui, additional, Adjie, Bayu, additional, and Yan, Yue-Hong, additional
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- 2017
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9. Impact of Chemotherapy Delay on Overall Survival for AML with IDH1/2 Mutations: A Study in Adult Chinese Patients
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Jian-Hu Li, Liya Ma, Hongyan Tong, Zhixin Ma, Juying Wei, Yue-Hong Jiang, Hui Shi, Min Yang, Ying Li, Jian Huang, Gaixiang Xu, Jinghan Wang, Qiuling Ma, Shasha Lu, Wenjuan Yu, Huanping Wang, Hong-Qiong Xie, Liping Mao, Dongmei Wang, Xiufeng Yin, Jie Jin, Qi Guo, Yejiang Lou, Mengxia Yu, and Dan Shen
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Oncology ,Adult ,Male ,NPM1 ,medicine.medical_specialty ,China ,IDH1 ,Time Factors ,Adolescent ,medicine.medical_treatment ,lcsh:Medicine ,Context (language use) ,medicine.disease_cause ,Young Adult ,Asian People ,Internal medicine ,CEBPA ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,lcsh:Science ,Survival analysis ,Aged ,Aged, 80 and over ,Chemotherapy ,Mutation ,Multidisciplinary ,business.industry ,lcsh:R ,Myeloid leukemia ,Middle Aged ,Prognosis ,Isocitrate Dehydrogenase ,Leukemia, Myeloid, Acute ,Immunology ,lcsh:Q ,Female ,business ,Nucleophosmin ,Research Article - Abstract
The effect of time from diagnosis to treatment (TDT) on overall survival of patients with acute myeloid leukemia (AML) remains obscure. Furthermore, whether chemotherapy delay impacts overall survival (OS) of patients with a special molecular subtype has not been investigated. Here, we enrolled 364 cases of AML to assess the effect of TDT on OS by fractional polynomial regression in the context of clinical parameters and genes of FLT3ITD, NPM1, CEBPA, DNMT3a, and IDH1/2 mutations. Results of the current study show IDH1/2 mutations are associated with older age, M0 morphology, an intermediate cytogenetic risk group, and NPM1 mutations. TDT associates with OS for AML patients in a nonlinear pattern with a J shape. Moreover, adverse effect of delayed treatment on OS was observed in patients with IDH1/2 mutations, but not in those with IDH1/2 wildtype. Therefore, initiating chemotherapy as soon as possible after diagnosis might be a potential strategy to improve OS in AML patients with IDH1/2 mutations.
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- 2015
10. Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice
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Hua Wang, Yipeng Xu, Zhijian Cai, Gu Zhang, Shaoxing Zhu, Ying Su, Fei Yang, Yue-Hong Shen, and An Zhao
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0301 basic medicine ,Male ,Integrins ,Adenoviruses ,Cancer Treatment ,lcsh:Medicine ,Pathology and Laboratory Medicine ,Adenovirus E1B protein ,Prostate cancer ,Mice ,0302 clinical medicine ,Spectrum Analysis Techniques ,Cytopathogenic Effect, Viral ,Prostate ,Medicine and Health Sciences ,lcsh:Science ,Adenovirus E1B Proteins ,DU145 cells ,Multidisciplinary ,Chemistry ,Prostate Cancer ,Prostate Diseases ,Animal Models ,Flow Cytometry ,Extracellular Matrix ,Oncolytic Viruses ,medicine.anatomical_structure ,Oncology ,Medical Microbiology ,Spectrophotometry ,030220 oncology & carcinogenesis ,Viral Pathogens ,Viruses ,Cell lines ,Adenovirus E1A Proteins ,Cytophotometry ,Cellular Structures and Organelles ,Pathogens ,Biological cultures ,Oligopeptides ,Research Article ,Oncolytic adenovirus ,Urology ,Mouse Models ,In Vitro Techniques ,Microbiology ,Adenoviridae ,03 medical and health sciences ,Model Organisms ,DU145 ,Cell Line, Tumor ,LNCaP ,medicine ,Cell Adhesion ,Animals ,Humans ,Adenovirus infection ,Microbial Pathogens ,lcsh:R ,Organisms ,Prostatic Neoplasms ,Cancers and Neoplasms ,Biology and Life Sciences ,Cell Biology ,medicine.disease ,Virology ,Oncolytic virus ,Research and analysis methods ,Genitourinary Tract Tumors ,030104 developmental biology ,Mutation ,Cancer research ,lcsh:Q ,DNA viruses - Abstract
CAR is a transmembrane protein that is expressed in various epithelial and endothelial cells. CAR mediates adenoviral infection, as well as adenovirus-mediated oncolysis of AxdAdB-3, an E1A/E1B double-restricted oncolytic adenovirus, in prostate cancer cells. This study further assessed the therapeutic efficacy of AxdAdB-3 with Arg-Gly-Asp (RGD)-fiber modification (AxdAdB3-F/RGD), which enables integrin-dependent infection, in prostate cancer. Susceptibility of prostate cancer cells LNCaP, PC3, and DU145 to adenovirus infection was associated with CAR expression. All of the prostate cancer cell lines expressed integrin αvβ3 and αvβ5. AxdAdB-3 was more cytopathic in CAR-positive prostate cancer cells than in CAR-negative cells, whereas AxdAdB3-F/RGD caused potent oncolysis in both CAR-positive and CAR-negative prostate cancer cells. In contrast, AxdAdB3-F/RGD was not cytopathic against normal prostate epithelial cells, RWPE-1. Intratumoral injection of AxdAdB3-F/RGD into CAR-negative prostate cancer cell xenografts in nude mice inhibited tumor growth. The current study demonstrates that E1A/E1B double-restricted oncolytic adenovirus with an RGD-fiber modification enhances infection efficiency and anti-tumor activity in CAR-deficient prostate cancer cells, while sparing normal cells. Future studies will evaluate the therapeutic potential of AxdAdB3-F/RGD in prostate cancer.
- Published
- 2015
11. Re-establishment of species from synonymies based on DNA barcoding and phylogenetic analysis using Diplopterygium simulans (Gleicheniaceae) as an example
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Yu-Feng Gu, Rui Zhang, Yue-Hong Yan, Hong-Mei Liu, Ying Wang, Hui Shen, Dongmei Jin, Bayu Adjie, Hong-Jin Wei, Jiang-Ping Shu, Faguo Wang, Xi-Le Zhou, and Hui Shang
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0106 biological sciences ,0301 basic medicine ,Species Delimitation ,Molecular biology ,Synonym ,Speciation ,Population Dynamics ,lcsh:Medicine ,Evolutionary biology ,Artificial Gene Amplification and Extension ,Polymerase Chain Reaction ,01 natural sciences ,DNA barcoding ,Genus ,lcsh:Science ,Phylogeny ,Molecular systematics ,Computer and information sciences ,Multidisciplinary ,Phylogenetic tree ,Database and informatics methods ,Sequence analysis ,Phylogenetic Analysis ,Plants ,Insects ,Phylogenetics ,Drosophila ,Identification (biology) ,Research Article ,Species complex ,Evolutionary Processes ,DNA, Plant ,Arthropoda ,Bioinformatics ,Evolutionary systematics ,Zoology ,Biology ,Data management ,010603 evolutionary biology ,Fruits ,03 medical and health sciences ,DNA Barcoding, Taxonomic ,Animals ,Gleicheniaceae ,DNA sequence analysis ,Taxonomy ,Population Biology ,lcsh:R ,Organisms ,Biology and Life Sciences ,Figs ,biology.organism_classification ,Invertebrates ,Geographic Distribution ,Research and analysis methods ,Molecular biology techniques ,030104 developmental biology ,Genetic distance ,Ferns ,lcsh:Q - Abstract
Because synonymy treatment traditionally relies on morphological judgments, it usually causes many problems in species delimitation and in the biodiversity catalogue. For example, Diplopterygium simulans, which belongs to the Gleicheniaceae family, has been considered to be synonymous with D. glaucum or D. giganteum based mainly on the morphology of its pinna rachis and blade. In the absence of molecular evidence, these revisions remain doubtful. DNA barcoding, which is considered to be a powerful method for species-level identification, was employed to assess the genetic distance among 9 members of the Diplopterygium genus. The results indicate that D. simulans is an independent species rather than a synonymy of D. glaucum or D. giganteum. Moreover, phylogenetic analysis uncovered the sisterhood of D. simulans and D. cantonense, which is supported by their geographical distributions and morphological traits. Incorrect synonymy treatment is prevalent in the characterization of biological diversity, and our study proposes a convenient and effective method for validating synonym treatments and discovering cryptic species.
- Published
- 2017
12. An alternative suite of universal primers for genotyping in multiplex PCR
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Yu-Nan Cui, Peng-Yu Jing, Cheng Ge, and Xiao-Yue Hong
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Heredity ,Genotyping Techniques ,Population ,Gene Identification and Analysis ,Population genetics ,lcsh:Medicine ,Biology ,law.invention ,Molecular Genetics ,law ,Multiplex polymerase chain reaction ,Genetics ,Animals ,Multiplex ,education ,Molecular Biology Techniques ,Sequencing Techniques ,lcsh:Science ,Genotyping ,Molecular Biology ,Polymerase chain reaction ,DNA Primers ,education.field_of_study ,Mites ,Evolutionary Biology ,Multidisciplinary ,Population Biology ,lcsh:R ,Biology and Life Sciences ,Computational Biology ,Genetic Loci ,Microsatellite ,Female ,lcsh:Q ,Multiplex Polymerase Chain Reaction ,Sequence Analysis ,Zoology ,Entomology ,Population Genetics ,Microsatellite Repeats ,Research Article ,Biotechnology - Abstract
The universal primer three-primer approach can dramatically reduce the cost when genotyping the microsatellites. One former research reported four universal primers that can be used in singleplex and multiplex genotyping. In this study, we proposed an alternative suite of universal primers with four dyes for genotyping 8–12 loci in one single run. This multiplex method was tested on Tetranychus truncatus. Published microsatellite loci of T. kanzawai, Frankliniella occidentalis and Nilaparvata lugens were modified as needed and also tested. The robustness of the method was confirmed by comparing with singleplex using multiple fluorophores and genotyping two populations of T. truncatus. This method showed lower signal strength than the singleplex three-primer system, but it was still sufficient to determine the fragment length. The cost of such a project can be reduced dramatically when many loci of different species are involved. In this way, laboratories performing population genetic analyses or studying several different species may benefit from the use of this cost-effective protocol.
- Published
- 2014
13. Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice
- Author
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Shen, Yue-Hong, primary, Yang, Fei, additional, Wang, Hua, additional, Cai, Zhi-Jian, additional, Xu, Yi-Peng, additional, Zhao, An, additional, Su, Ying, additional, Zhang, Gu, additional, and Zhu, Shao-Xing, additional
- Published
- 2016
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14. Impact of Chemotherapy Delay on Overall Survival for AML with IDH1/2 Mutations: A Study in Adult Chinese Patients
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Wang, Jing-Han, primary, Guo, Qi, additional, Ma, Zhi-Xin, additional, Ma, Qiu-Ling, additional, Yu, Meng-Xia, additional, Yin, Xiu-Feng, additional, Lu, Sha-Sha, additional, Xie, Hong-Qiong, additional, Jiang, Yue-Hong, additional, Shen, Dan, additional, Ma, Li-Ya, additional, Shi, Hui, additional, Yu, Wen-Juan, additional, Lou, Ye-Jiang, additional, Li, Ying, additional, Yang, Min, additional, Xu, Gai-Xiang, additional, Mao, Li-Ping, additional, Li, Jian-Hu, additional, Wang, Huan-Ping, additional, Wang, Dong-Mei, additional, Wei, Ju-Ying, additional, Tong, Hong-Yan, additional, Huang, Jian, additional, and Jin, Jie, additional
- Published
- 2015
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15. Wolbachia-Host Interactions: Host Mating Patterns Affect Wolbachia Density Dynamics
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Yan-Kai Zhang, Da-Song Chen, Xiao-Yue Hong, Dong-Xiao Zhao, and Xiang-Fei Zhang
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Evolutionary Genetics ,Male ,Colony Count, Microbial ,Zoology ,lcsh:Medicine ,Plant Science ,Microbiology ,Polymerase Chain Reaction ,Microbial Ecology ,Sexual Behavior, Animal ,Spider mite ,parasitic diseases ,Animals ,Tetranychus urticae ,Mating ,lcsh:Science ,Biology ,In Situ Hybridization, Fluorescence ,Tropism ,reproductive and urinary physiology ,Evolutionary Biology ,Plant Pests ,Multidisciplinary ,Ecology ,biology ,Host (biology) ,Intracellular parasite ,lcsh:R ,Plant Pathology ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Host-Pathogen Interaction ,Evolutionary Ecology ,Genes, Bacterial ,Host-Pathogen Interactions ,bacteria ,Parasitology ,Female ,Wolbachia ,lcsh:Q ,Tetranychidae ,Cytoplasmic incompatibility ,Research Article ,Multilocus Sequence Typing - Abstract
Wolbachia are maternally inherited intracellular bacteria that infect a wide range of arthropods and cause an array of effects on host reproduction, fitness and mating behavior. Although our understanding of the Wolbachia-associated effects on hosts is rapidly expanding, our knowledge of the host factors that mediate Wolbachia dynamics is rudimentary. Here, we explore the interactions between Wolbachia and its host, the two-spotted spider mite Tetranychus urticae Koch. Our results indicate that Wolbachia induces strong cytoplasmic incompatibility (CI), increases host fecundity, but has no effects on the longevity of females and the mating competitiveness of males in T. urticae. Most importantly, host mating pattern was found to affect Wolbachia density dynamics during host aging. Mating of an uninfected mite of either sex with an infected mite attenuates the Wolbachia density in the infected mite. According to the results of Wolbachia localization, this finding may be associated with the tropism of Wolbachia for the reproductive tissue in adult spider mites. Our findings describe a new interaction between Wolbachia and their hosts.
- Published
- 2013
16. The Complete Mitochondrial Genomes of Six Species of Tetranychus Provide Insights into the Phylogeny and Evolution of Spider Mites
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Peng-Yu Jin, Kai-Jun Zhang, Jia-Fei Ju, Da-Song Chen, Si-Xia Yang, Xiu-Lei Ding, Jing-Yu Zhao, and Xiao-Yue Hong
- Subjects
Mitochondrial DNA ,Genome evolution ,lcsh:Medicine ,Biology ,DNA, Mitochondrial ,Genome ,Molecular Evolution ,Evolution, Molecular ,RNA, Transfer ,Phylogenetics ,Molecular evolution ,Animals ,Tetranychus urticae ,lcsh:Science ,Genome size ,Phylogeny ,Taxonomy ,Genetics ,Evolutionary Biology ,Multidisciplinary ,Base Sequence ,lcsh:R ,Biology and Life Sciences ,Sequence Analysis, DNA ,biology.organism_classification ,Animal Taxonomy ,Genome, Mitochondrial ,Nucleic Acid Conformation ,lcsh:Q ,Tetranychus ,Tetranychidae ,Zoology ,Entomology ,Research Article - Abstract
Many spider mites belonging to the genus Tetranychus are of agronomical importance. With limited morphological characters, Tetranychus mites are usually identified by a combination of morphological characteristics and molecular diagnostics. To clarify their molecular evolution and phylogeny, the mitochondrial genomes of the green and red forms of Tetranychus urticae as well as T. kanzawai, T. ludeni, T. malaysiensis, T. phaselus, T. pueraricola were sequenced and compared. The seven mitochondrial genomes are typical circular molecules of about 13,000 bp encoding and they are composed of the complete set of 37 genes that are usually found in metazoans. The order of the mitochondrial (mt) genes is the same as that in the mt genomes of Panonychus citri and P. ulmi, but very different from that in other Acari. The J-strands of the mitochondrial genomes have high (∼ 84%) A+T contents, negative GC-skews and positive AT-skews. The nucleotide sequence of the cox1 gene, which is commonly used as a taxon barcode and molecular marker, is more highly conserved than the nucleotide sequences of other mitochondrial genes in these seven species. Most tRNA genes in the seven genomes lose the D-arm and/or the T-arm. The functions of these tRNAs need to be evaluated. The mitochondrial genome of T. malaysiensis differs from the other six genomes in having a slightly smaller genome size, a slight difference in codon usage, and a variable loop in place of the T-arm of some tRNAs by a variable loop. A phylogenic analysis shows that T. malaysiensis first split from other Tetranychus species and that the clade of the family Tetranychoidea occupies a basal position in the Trombidiformes. The mt genomes of the green and red forms of T. urticae have limited divergence and short evolutionary distance.
- Published
- 2014
17. Notch γ-Secretase Inhibitor Dibenzazepine Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm in ApoE Knockout Mice by Multiple Mechanisms
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Zheng, Yue-Hong, primary, Li, Fang-Da, additional, Tian, Cui, additional, Ren, Hua-Liang, additional, Du, Jie, additional, and Li, Hui-Hua, additional
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- 2013
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18. Multiple Infections with Cardinium and Two Strains of Wolbachia in The Spider Mite Tetranychus phaselus Ehara: Revealing New Forces Driving the Spread of Wolbachia
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Cheng Ge, Tetsuo Gotoh, Xiao-Yue Hong, Da-Song Chen, and Dong-Xiao Zhao
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Male ,Bacteroidaceae ,media_common.quotation_subject ,Population ,lcsh:Medicine ,Plant Science ,Biology ,Microbiology ,Microbial Ecology ,Spider mite ,Animals ,lcsh:Science ,education ,media_common ,Genetics ,Evolutionary Biology ,Plant Pests ,education.field_of_study ,Multidisciplinary ,Ecology ,Coinfection ,Host (biology) ,lcsh:R ,Longevity ,Agriculture ,Bacterial Infections ,Plant Pathology ,biology.organism_classification ,Virology ,Multiple infections ,Evolutionary Ecology ,lcsh:Q ,Female ,Wolbachia ,Pest Control ,Tetranychidae ,Zoology ,Entomology ,Tetranychus phaselus ,Cytoplasmic incompatibility ,Research Article - Abstract
Cytoplasmic incompatibility (CI) has been proposed as a major mechanism by which certain strains of Wolbachia to invade and persist in host populations. However, mechanisms that underlie the invasion and persistence of non-CI strains are less well understood. Here, we established a spider mite Tetranychus phaselus population multiply infected by Cardinium as well as two distinct lineages of Wolbachia, designated wCon and wOri, to study the forces driving the spread of the non-CI strain of Wolbachia wOri. Interestingly, we found that wOri provided a longevity advantage to its female hosts under ideal conditions, making wOri stay longer in this population, and then being transmitted to more offspring. Furthermore, the lifespan of uninfected females was reduced when mated with multiple-infected males. As a result, the uninfected population is attenuated by the multiple-infected males. Thus, we infer that the host age effects of multiple infection may represent sufficient forces driving the spread of wOri through the host population.
- Published
- 2013
19. Smyd1b_tv1, a Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers
- Author
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Li, Huiqing, primary, Xu, Jin, additional, Bian, Yue-Hong, additional, Rotllant, Pep, additional, Shen, Tiansheng, additional, Chu, Wuying, additional, Zhang, Jianshe, additional, Schneider, Martin, additional, and Du, Shao Jun, additional
- Published
- 2011
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20. Invasion Genetics of the Western Flower Thrips in China: Evidence for Genetic Bottleneck, Hybridization and Bridgehead Effect
- Author
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Xian-Ming Yang, Xiao-Feng Xue, Jin-Bo Li, Jing-Tao Sun, and Xiao-Yue Hong
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Gene Flow ,Evolutionary Genetics ,Male ,China ,Species complex ,Population Dynamics ,lcsh:Medicine ,Population genetics ,Crops ,Genes, Insect ,Plant Science ,Biology ,Gene flow ,Evolution, Molecular ,Genetics ,Animals ,lcsh:Science ,Phylogeny ,Evolutionary Biology ,Plant Pests ,Genetic diversity ,Polymorphism, Genetic ,Multidisciplinary ,Ecology ,Ecotype ,lcsh:R ,Thysanoptera ,Computational Biology ,Agriculture ,Plant Pathology ,biology.organism_classification ,Western flower thrips ,Mitochondria ,Population bottleneck ,Genetic structure ,Hybridization, Genetic ,lcsh:Q ,Female ,Pest Control ,Population Ecology ,Introduced Species ,Population Genetics ,Research Article ,Microsatellite Repeats - Abstract
The western flower thrips, Frankliniella occidentalis (Pergande), is an invasive species and the most economically important pest within the insect order Thysanoptera. F. occidentalis, which is endemic to North America, was initially detected in Kunming in southwestern China in 2000 and since then it has rapidly invaded several other localities in China where it has greatly damaged greenhouse vegetables and ornamental crops. Controlling this invasive pest in China requires an understanding of its genetic makeup and migration patterns. Using the mitochondrial COI gene and 10 microsatellites, eight of which were newly isolated and are highly polymorphic, we investigated the genetic structure and the routes of range expansion of 14 F. occidentalis populations in China. Both the mitochondrial and microsatellite data revealed that the genetic diversity of F. occidentalis of the Chinese populations is lower than that in its native range. Two previously reported cryptic species (or ecotypes) were found in the study. The divergence in the mitochondrial COI of two Chinese cryptic species (or ecotypes) was about 3.3% but they cannot be distinguished by nuclear markers. Hybridization might produce such substantial mitochondrial-nuclear discordance. Furthermore, we found low genetic differentiation (global F(ST) = 0.043, P
- Published
- 2012
21. Smyd1b_tv1, a Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers
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Shaojun Du, Yue-Hong Bian, Huiqing Li, Martin F. Schneider, Jin Xu, Pep Rotllant, Jianshe Zhang, Tiansheng Shen, and Wuying Chu
- Subjects
Aging ,Embryo, Nonmammalian ,Time Factors ,Muscle Fibers, Skeletal ,Gene Expression ,lcsh:Medicine ,Muscle Development ,Biochemistry ,Sarcomere ,Myofibrils ,Molecular Cell Biology ,Serine ,Protein Isoforms ,Myocyte ,lcsh:Science ,Zebrafish ,Peptide sequence ,Cytoskeleton ,Multidisciplinary ,biology ,Myogenesis ,Muscle cell differentiation ,Cell Differentiation ,Animal Models ,Cellular Structures ,Protein Transport ,Gene Knockdown Techniques ,Subcellular Fractions ,Research Article ,Sarcomeres ,Phenylalanine ,Recombinant Fusion Proteins ,Green Fluorescent Proteins ,Molecular Sequence Data ,Model Organisms ,Genetics ,Animals ,Amino Acid Sequence ,HSP90 Heat-Shock Proteins ,Protein Interactions ,Biology ,lcsh:R ,Alternative splicing ,Proteins ,Histone-Lysine N-Methyltransferase ,Zebrafish Proteins ,Subcellular localization ,biology.organism_classification ,Molecular biology ,Alternative Splicing ,Mutagenesis, Insertional ,Protein Translation ,lcsh:Q - Abstract
12 páginas, 7 figuras, Background Smyd1b is a member of the Smyd family that plays a key role in sarcomere assembly during myofibrillogenesis. Smyd1b encodes two alternatively spliced isoforms, smyd1b_tv1 and smyd1b_tv2, that are expressed in skeletal and cardiac muscles and play a vital role in myofibrillogenesis in skeletal muscles of zebrafish embryos. Methodology/Principal Findings To better understand Smyd1b function in myofibrillogenesis, we analyzed the subcellular localization of Smyd1b_tv1 and Smyd1b_tv2 in transgenic zebrafish expressing a myc-tagged Smyd1b_tv1 or Smyd1b_tv2. The results showed a dynamic change of their subcellular localization during muscle cell differentiation. Smyd1b_tv1 and Smyd1b_tv2 were primarily localized in the cytosol of myoblasts and myotubes at early stage zebrafish embryos. However, in mature myofibers, Smyd1b_tv1, and to a small degree of Smyd1b_tv2, exhibited a sarcomeric localization. Double staining with sarcomeric markers revealed that Smyd1b_tv1was localized on the M-lines. The sarcomeric localization was confirmed in zebrafish embryos expressing the Smyd1b_tv1-GFP or Smyd1b_tv2-GFP fusion proteins. Compared with Smyd1b_tv1, Smyd1b_tv2, however, showed a weak sarcomeric localization. Smyd1b_tv1 differs from Smyd1b_tv2 by a 13 amino acid insertion encoded by exon 5, suggesting that some residues within the 13 aa insertion may be critical for the strong sarcomeric localization of Smyd1b_tv1. Sequence comparison with Smyd1b_tv1 orthologs from other vertebrates revealed several highly conserved residues (Phe223, His224 and Gln226) and two potential phosphorylation sites (Thr221 and Ser225) within the 13 aa insertion. To determine whether these residues are involved in the increased sarcomeric localization of Smyd1b_tv1, we mutated these residues into alanine. Substitution of Phe223 or Ser225 with alanine significantly reduced the sarcomeric localization of Smyd1b_tv1. In contrast, other substitutions had no effect. Moreover, replacing Ser225 with threonine (S225T) retained the strong sarcomeric localization of Smyd1b_tv1. Conclusion/Significance Together, these data indicate that Phe223 and Ser225 are required for the M-line localization of Smyd1b_tv1., This research was supported by research grant No IS-8713-08 from the Israel Binational Agricultural Research and Development Fund, the United States (BARD), and an intercenter collaboration grant (Du-Fang) from University of Maryland Biotechnology Institute. (http://www.bard-isus.com/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Published
- 2011
22. Multiple Infections with Cardinium and Two Strains of Wolbachia in The Spider Mite Tetranychus phaselus Ehara: Revealing New Forces Driving the Spread of Wolbachia.
- Author
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Dong-Xiao Zhao, Da-Song Chen, Cheng Ge, Tetsuo Gotoh, and Xiao-Yue Hong
- Subjects
WOLBACHIA ,SPIDER mites ,TETRANYCHUS ,INFECTION ,CYTOPLASM ,ACARIFORMES - Abstract
Cytoplasmic incompatibility (CI) has been proposed as a major mechanism by which certain strains of Wolbachia to invade and persist in host populations. However, mechanisms that underlie the invasion and persistence of non-CI strains are less well understood. Here, we established a spider mite Tetranychus phaselus population multiply infected by Cardinium as well as two distinct lineages of Wolbachia, designated wCon and wOri, to study the forces driving the spread of the non-CI strain of Wolbachia wOri. Interestingly, we found that wOri provided a longevity advantage to its female hosts under ideal conditions, making wOri stay longer in this population, and then being transmitted to more offspring. Furthermore, the lifespan of uninfected females was reduced when mated with multiple-infected males. As a result, the uninfected population is attenuated by the multiple-infected males. Thus, we infer that the host age effects of multiple infection may represent sufficient forces driving the spread of wOri through the host population. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
23. Smyd1ba Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers.
- Author
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Huiqing Li, Jin Xu, Bian, Yue-Hong, Pep Rotllant, Tiansheng Shen, Wuying Chu, Jianshe Zhang, Schneider, Martin, and Shao Jun Du
- Subjects
MUSCLES ,ZEBRA danio ,MUSCLE cells ,CHORDATA ,CYTOSOL ,MYOBLASTS ,CELL differentiation - Abstract
Background: Smyd1b is a member of the Smyd family that plays a key role in sarcomere assembly during myofibrillogenesis. Smyd1b encodes two alternatively spliced isoforms, smyd1b_tv1 and smyd1b_tv2, that are expressed in skeletal and cardiac muscles and play a vital role in myofibrillogenesis in skeletal muscles of zebrafish embryos. Methodology/Principal Findings: To better understand Smyd1b function in myofibrillogenesis, we analyzed the subcellular localization of Smyd1b_tv1 and Smyd1b_tv2 in transgenic zebrafish expressing a myc-tagged Smyd1b_tv1 or Smyd1b_tv2. The results showed a dynamic change of their subcellular localization during muscle cell differentiation. Smyd1b_tv1 and Smyd1b_tv2 were primarily localized in the cytosol of myoblasts and myotubes at early stage zebrafish embryos. However, in mature myofibers, Smyd1b_tv1, and to a small degree of Smyd1b_tv2, exhibited a sarcomeric localization. Double staining with sarcomeric markers revealed that Smyd1b_tv1was localized on the M-lines. The sarcomeric localization was confirmed in zebrafish embryos expressing the Smyd1b_tv1-GFP or Smyd1b_tv2-GFP fusion proteins. Compared with Smyd1b_tv1, Smyd1b_tv2, however, showed a weak sarcomeric localization. Smyd1b_tv1 differs from Smyd1b_tv2 by a 13 amino acid insertion encoded by exon 5, suggesting that some residues within the 13 aa insertion may be critical for the strong sarcomeric localization of Smyd1b_tv1. Sequence comparison with Smyd1b_tv1 orthologs from other vertebrates revealed several highly conserved residues (Phe223, His224 and Gln226) and two potential phosphorylation sites (Thr221 and Ser225) within the 13 aa insertion. To determine whether these residues are involved in the increased sarcomeric localization of Smyd1b_tv1, we mutated these residues into alanine. Substitution of Phe223 or Ser225 with alanine significantly reduced the sarcomeric localization of Smyd1b_tv1. In contrast, other substitutions had no effect. Moreover, replacing Ser225 with threonine (S225T) retained the strong sarcomeric localization of Smyd1b_tv1. Conclusion/Significance: Together, these data indicate that Phe223 and Ser225 are required for the M-line localization of Smyd1b_tv1. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
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24. Lactobacillus plantarum improves LPS-induced Caco2 cell line intestinal barrier damage via cyclic AMP-PKA signaling
- Author
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Chen-Xiang Wei, Ju-Hua Wu, Yue-Hong Huang, Xiao-Zhong Wang, and Jian-Ying Li
- Subjects
Medicine ,Science - Abstract
Lactobacillus plantarum (LP) has been shown to exhibit protective effects on intestinal barrier function in septic rats, although the regulatory mechanism has not been established. We determined whether LP imparts such protective effects in a lipopolysaccharide (LPS)-induced Caco2 cell monolayer model and whether cAMP-PKA signaling is the underlying mechanism of action. The cyclic adenosine monophosphate (cAMP) agonist, forskolin (FSK), and the protein kinase A (PKA) inhibitor, HT89, were used to study the protective effect of LP on the destruction of the tight junction (TJ) structure of cells treated with LPS and the corresponding changes in cAMP-PKA signaling. Our experimental results demonstrated that LP promoted the expression of TJ proteins between Caco2 cells after LPS treatment, and increased the electrical barrier detection (TEER) between Caco2 cells. Moreover, transmission electron microscopy (TEM) revealed that the TJ structural integrity of cells treated with LPS + LP was improved compared to cells treated with LPS alone. In addition, our findings were consistent between the FSK and LP intervention group, while HT89 inhibited LP influence. Taken together, our results indicate that LP has an improved protective effect on LPS-induced damage to the monolayer membrane barrier function of Caco2 cells and is regulated by the cAMP-PKA pathway.
- Published
- 2022
25. Revision of series Gravesiana (Adiantum L.) based on morphological characteristics, spores and phylogenetic analyses.
- Author
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Ai-Hua Wang, Fa-Guo Wang, Wan-Wan Zhang, Xiao-Dong Ma, Xu-Wen Li, Qi-Fei Yi, Dong-Lin Li, Lei Duan, Yue-Hong Yan, and Fu-Wu Xing
- Subjects
Medicine ,Science - Abstract
Since the adoption of some ambiguous and quantitative characters in Flora Republicae Popularis Sinicae 3(1), species identifications of the series Gravesiana have been in disarray, requiring clarification. Two hundred and fifty-nine individuals from 47 different populations were collected for the estimation of morphological characters and phylogenetic analyses. Spores of 26 populations were observed through scanning electron microscope. Our results were different from those of previous research: (1) six identifiable species, rather than five species observed previously, were confirmed in the series Gravesiana, they are A. gravesii, A. juxtapositum, A. mariesii, A. dentatum, A. longzhouensis and A. obovatum, of which the latter three are newly recognized species. (2) Thirteen characters were measured and estimated through the program Mesquite v. 2.71. The character whether the pinna stalks were 1/3-1/2 times longer than the pinna was used to distinguish A. gravesii and A. lianxianense previously and was found to be unreliable here, whereas such characters as the height of the plant (H), pinna aligned forms (FP), number of pinna (NP), pinna margin (M), number of veins flabellate at base (NV), sori number and shape per pinna (NSS), pinna texture (T), and powder-covered or not on the abaxial surface of the pinna (P) are estimated to be stable and reliable characters useful for identification. Descriptions of new species and their retrieve keys are also listed. (3) Surface ornamentations and spore sizes are helpful for us to distinguish species in series Gravesiana.
- Published
- 2017
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26. Physiological and genomic features of a novel violacein-producing bacterium isolated from surface seawater.
- Author
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Yue-Hong Wu, Hong Cheng, Lin Xu, Xiong-Bin Jin, Chun-Sheng Wang, and Xue-Wei Xu
- Subjects
Medicine ,Science - Abstract
Strains JW1T and JW3, isolated from surface seawater of the Arabian Sea, were subjected to polyphasic taxonomic analysis. Cells of both strains were Gram-stain-negative, aerobic, and rod-shaped. They formed violet pigment and produced violacein. On the basis of 16S rRNA gene sequence analysis, strains JW1T and JW3 showed high 16S rRNA gene sequence similarity with Pseudoalteromonas byunsanensis JCM12483T (98.2%), P. shioyasakiensis SE3T (97.8%), P. arabiensis JCM 17292T (97.3%), and P. gelatinilytica NH153T (97.1%). The 16S rRNA gene sequence similarity between JW1T and JW3 was 100%. Phylogenetic analyses revealed that both strains fell within the cluster of the genus Pseudoalteromonas and represented an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between JW1T and type strains of the closely related Pseudoalteromonas species were 70.9-83.3% and 20.0-26.4%, respectively. The sole respiratory quinone in both strains is ubiquinone 8 (Q-8). The principal fatty acids are summed feature 3 (C16:1ω7c and/or iso-C15:0 2OH), C18:1ω7c, and C16:0. The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, one unidentified glycolipid, one unidentified aminolipid, and one unidentified phospholipid. The DNA G+C content was 43.3 mol%. Differential phylogenetic distinctiveness, chemotaxonomic differences, and phenotypic properties indicated that strains JW1T and JW3 could be differentiated from the Pseudoalteromonas species with validly published names. Therefore, it is proposed that strains JW1T and JW3 represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas amylolytica sp. nov. (type strain, JW1T = CGMCC 1.15681T = KCTC 52406T = MCCC 1K02162T) is proposed.
- Published
- 2017
- Full Text
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27. Re-establishment of species from synonymies based on DNA barcoding and phylogenetic analysis using Diplopterygium simulans (Gleicheniaceae) as an example.
- Author
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Jiang-Ping Shu, Hui Shang, Dongmei Jin, Hong-Jin Wei, Xi-Le Zhou, Hong-Mei Liu, Yu-Feng Gu, Ying Wang, Fa-Guo Wang, Hui Shen, Rui Zhang, Bayu Adjie, and Yue-Hong Yan
- Subjects
Medicine ,Science - Abstract
Because synonymy treatment traditionally relies on morphological judgments, it usually causes many problems in species delimitation and in the biodiversity catalogue. For example, Diplopterygium simulans, which belongs to the Gleicheniaceae family, has been considered to be synonymous with D. glaucum or D. giganteum based mainly on the morphology of its pinna rachis and blade. In the absence of molecular evidence, these revisions remain doubtful. DNA barcoding, which is considered to be a powerful method for species-level identification, was employed to assess the genetic distance among 9 members of the Diplopterygium genus. The results indicate that D. simulans is an independent species rather than a synonymy of D. glaucum or D. giganteum. Moreover, phylogenetic analysis uncovered the sisterhood of D. simulans and D. cantonense, which is supported by their geographical distributions and morphological traits. Incorrect synonymy treatment is prevalent in the characterization of biological diversity, and our study proposes a convenient and effective method for validating synonym treatments and discovering cryptic species.
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- 2017
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28. Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice.
- Author
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Yue-Hong Shen, Fei Yang, Hua Wang, Zhi-Jian Cai, Yi-Peng Xu, An Zhao, Ying Su, Gu Zhang, and Shao-Xing Zhu
- Subjects
Medicine ,Science - Abstract
CAR is a transmembrane protein that is expressed in various epithelial and endothelial cells. CAR mediates adenoviral infection, as well as adenovirus-mediated oncolysis of AxdAdB-3, an E1A/E1B double-restricted oncolytic adenovirus, in prostate cancer cells. This study further assessed the therapeutic efficacy of AxdAdB-3 with Arg-Gly-Asp (RGD)-fiber modification (AxdAdB3-F/RGD), which enables integrin-dependent infection, in prostate cancer. Susceptibility of prostate cancer cells LNCaP, PC3, and DU145 to adenovirus infection was associated with CAR expression. All of the prostate cancer cell lines expressed integrin αvβ3 and αvβ5. AxdAdB-3 was more cytopathic in CAR-positive prostate cancer cells than in CAR-negative cells, whereas AxdAdB3-F/RGD caused potent oncolysis in both CAR-positive and CAR-negative prostate cancer cells. In contrast, AxdAdB3-F/RGD was not cytopathic against normal prostate epithelial cells, RWPE-1. Intratumoral injection of AxdAdB3-F/RGD into CAR-negative prostate cancer cell xenografts in nude mice inhibited tumor growth. The current study demonstrates that E1A/E1B double-restricted oncolytic adenovirus with an RGD-fiber modification enhances infection efficiency and anti-tumor activity in CAR-deficient prostate cancer cells, while sparing normal cells. Future studies will evaluate the therapeutic potential of AxdAdB3-F/RGD in prostate cancer.
- Published
- 2016
- Full Text
- View/download PDF
29. Impact of Chemotherapy Delay on Overall Survival for AML with IDH1/2 Mutations: A Study in Adult Chinese Patients.
- Author
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Jing-Han Wang, Qi Guo, Zhi-Xin Ma, Qiu-Ling Ma, Meng-Xia Yu, Xiu-Feng Yin, Sha-Sha Lu, Hong-Qiong Xie, Yue-Hong Jiang, Dan Shen, Li-Ya Ma, Hui Shi, Wen-Juan Yu, Ye-Jiang Lou, Ying Li, Min Yang, Gai-Xiang Xu, Li-Ping Mao, Jian-Hu Li, Huan-Ping Wang, Dong-Mei Wang, Ju-Ying Wei, Hong-Yan Tong, Jian Huang, and Jie Jin
- Subjects
Medicine ,Science - Abstract
The effect of time from diagnosis to treatment (TDT) on overall survival of patients with acute myeloid leukemia (AML) remains obscure. Furthermore, whether chemotherapy delay impacts overall survival (OS) of patients with a special molecular subtype has not been investigated. Here, we enrolled 364 cases of AML to assess the effect of TDT on OS by fractional polynomial regression in the context of clinical parameters and genes of FLT3ITD, NPM1, CEBPA, DNMT3a, and IDH1/2 mutations. Results of the current study show IDH1/2 mutations are associated with older age, M0 morphology, an intermediate cytogenetic risk group, and NPM1 mutations. TDT associates with OS for AML patients in a nonlinear pattern with a J shape. Moreover, adverse effect of delayed treatment on OS was observed in patients with IDH1/2 mutations, but not in those with IDH1/2 wildtype. Therefore, initiating chemotherapy as soon as possible after diagnosis might be a potential strategy to improve OS in AML patients with IDH1/2 mutations.
- Published
- 2015
- Full Text
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30. An alternative suite of universal primers for genotyping in multiplex PCR.
- Author
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Cheng Ge, Yu-Nan Cui, Peng-Yu Jing, and Xiao-Yue Hong
- Subjects
Medicine ,Science - Abstract
The universal primer three-primer approach can dramatically reduce the cost when genotyping the microsatellites. One former research reported four universal primers that can be used in singleplex and multiplex genotyping. In this study, we proposed an alternative suite of universal primers with four dyes for genotyping 8-12 loci in one single run. This multiplex method was tested on Tetranychus truncatus. Published microsatellite loci of T. kanzawai, Frankliniella occidentalis and Nilaparvata lugens were modified as needed and also tested. The robustness of the method was confirmed by comparing with singleplex using multiple fluorophores and genotyping two populations of T. truncatus. This method showed lower signal strength than the singleplex three-primer system, but it was still sufficient to determine the fragment length. The cost of such a project can be reduced dramatically when many loci of different species are involved. In this way, laboratories performing population genetic analyses or studying several different species may benefit from the use of this cost-effective protocol.
- Published
- 2014
- Full Text
- View/download PDF
31. The complete mitochondrial genomes of six species of Tetranychus provide insights into the phylogeny and evolution of spider mites.
- Author
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Da-Song Chen, Peng-Yu Jin, Kai-Jun Zhang, Xiu-Lei Ding, Si-Xia Yang, Jia-Fei Ju, Jing-Yu Zhao, and Xiao-Yue Hong
- Subjects
Medicine ,Science - Abstract
Many spider mites belonging to the genus Tetranychus are of agronomical importance. With limited morphological characters, Tetranychus mites are usually identified by a combination of morphological characteristics and molecular diagnostics. To clarify their molecular evolution and phylogeny, the mitochondrial genomes of the green and red forms of Tetranychus urticae as well as T. kanzawai, T. ludeni, T. malaysiensis, T. phaselus, T. pueraricola were sequenced and compared. The seven mitochondrial genomes are typical circular molecules of about 13,000 bp encoding and they are composed of the complete set of 37 genes that are usually found in metazoans. The order of the mitochondrial (mt) genes is the same as that in the mt genomes of Panonychus citri and P. ulmi, but very different from that in other Acari. The J-strands of the mitochondrial genomes have high (∼ 84%) A+T contents, negative GC-skews and positive AT-skews. The nucleotide sequence of the cox1 gene, which is commonly used as a taxon barcode and molecular marker, is more highly conserved than the nucleotide sequences of other mitochondrial genes in these seven species. Most tRNA genes in the seven genomes lose the D-arm and/or the T-arm. The functions of these tRNAs need to be evaluated. The mitochondrial genome of T. malaysiensis differs from the other six genomes in having a slightly smaller genome size, a slight difference in codon usage, and a variable loop in place of the T-arm of some tRNAs by a variable loop. A phylogenic analysis shows that T. malaysiensis first split from other Tetranychus species and that the clade of the family Tetranychoidea occupies a basal position in the Trombidiformes. The mt genomes of the green and red forms of T. urticae have limited divergence and short evolutionary distance.
- Published
- 2014
- Full Text
- View/download PDF
32. Notch γ-secretase inhibitor dibenzazepine attenuates angiotensin II-induced abdominal aortic aneurysm in ApoE knockout mice by multiple mechanisms.
- Author
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Yue-Hong Zheng, Fang-Da Li, Cui Tian, Hua-Liang Ren, Jie Du, and Hui-Hua Li
- Subjects
Medicine ,Science - Abstract
Abdominal aortic aneurysm (AAA) is a life-threatening aortic disease in the elderly. Activation of Notch1 pathway plays a critical role in the development of AAA, but the underlying mechanisms remain poorly understood. In the present study, we explored the mechanisms by which Notch1 activation regulates angiotensin II (Ang II)-induced AAA formation and evaluated the therapeutic potential of a new Notch γ-secretase inhibitor, dibenzazepine (DBZ), for the treatment of AAA. Apolipoprotein E knockout (Apo E(-/-)) mice infused for 4 weeks with Ang II (1000 ng/kg/min, IP) using osmotic mini-pumps were received an intraperitoneal injection of either vehicle or 1 mg/kg/d DBZ. Notch1 signaling was activated in AAA tissue from both Ang II-infused Apo E(-/-) mice and human undergoing AAA repair in vivo, with increased expression of Notch intracellular domain (NICD) and its target gene Hes1, and this effect was effectively blocked by DBZ. Moreover, infusion of Ang II markedly increased the incidence and severity of AAA in Apo E(-/-) mice. In contrast, inhibition of Notch activation by DBZ prevented AAA formation in vivo. Furthermore, DBZ markedly prevented Ang II-stimulated accumulation of macrophages and CD4(+) T cells, and ERK-mediated angiogenesis, simultaneously reversed Th2 response, in vivo. In conclusion, these findings provide new insight into the multiple mechanisms of Notch signaling involved in AAA formation and suggest that γ-secretase inhibitor DBZ might be a novel therapeutic drug for treating AAAS.
- Published
- 2013
- Full Text
- View/download PDF
33. Wolbachia-Host Interactions: Host Mating Patterns Affect Wolbachia Density Dynamics.
- Author
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Dong-Xiao Zhao, Xiang-Fei Zhang, Da-Song Chen, Yan-Kai Zhang, and Xiao-Yue Hong
- Subjects
Medicine ,Science - Abstract
Wolbachia are maternally inherited intracellular bacteria that infect a wide range of arthropods and cause an array of effects on host reproduction, fitness and mating behavior. Although our understanding of the Wolbachia-associated effects on hosts is rapidly expanding, our knowledge of the host factors that mediate Wolbachia dynamics is rudimentary. Here, we explore the interactions between Wolbachia and its host, the two-spotted spider mite Tetranychus urticae Koch. Our results indicate that Wolbachia induces strong cytoplasmic incompatibility (CI), increases host fecundity, but has no effects on the longevity of females and the mating competitiveness of males in T. urticae. Most importantly, host mating pattern was found to affect Wolbachia density dynamics during host aging. Mating of an uninfected mite of either sex with an infected mite attenuates the Wolbachia density in the infected mite. According to the results of Wolbachia localization, this finding may be associated with the tropism of Wolbachia for the reproductive tissue in adult spider mites. Our findings describe a new interaction between Wolbachia and their hosts.
- Published
- 2013
- Full Text
- View/download PDF
34. Smyd1b_tv1, a key regulator of sarcomere assembly, is localized on the M-line of skeletal muscle fibers.
- Author
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Huiqing Li, Jin Xu, Yue-Hong Bian, Pep Rotllant, Tiansheng Shen, Wuying Chu, Jianshe Zhang, Martin Schneider, and Shao Jun Du
- Subjects
Medicine ,Science - Abstract
BACKGROUND:Smyd1b is a member of the Smyd family that plays a key role in sarcomere assembly during myofibrillogenesis. Smyd1b encodes two alternatively spliced isoforms, smyd1b_tv1 and smyd1b_tv2, that are expressed in skeletal and cardiac muscles and play a vital role in myofibrillogenesis in skeletal muscles of zebrafish embryos. METHODOLOGY/PRINCIPAL FINDINGS:To better understand Smyd1b function in myofibrillogenesis, we analyzed the subcellular localization of Smyd1b_tv1 and Smyd1b_tv2 in transgenic zebrafish expressing a myc-tagged Smyd1b_tv1 or Smyd1b_tv2. The results showed a dynamic change of their subcellular localization during muscle cell differentiation. Smyd1b_tv1 and Smyd1b_tv2 were primarily localized in the cytosol of myoblasts and myotubes at early stage zebrafish embryos. However, in mature myofibers, Smyd1b_tv1, and to a small degree of Smyd1b_tv2, exhibited a sarcomeric localization. Double staining with sarcomeric markers revealed that Smyd1b_tv1was localized on the M-lines. The sarcomeric localization was confirmed in zebrafish embryos expressing the Smyd1b_tv1-GFP or Smyd1b_tv2-GFP fusion proteins. Compared with Smyd1b_tv1, Smyd1b_tv2, however, showed a weak sarcomeric localization. Smyd1b_tv1 differs from Smyd1b_tv2 by a 13 amino acid insertion encoded by exon 5, suggesting that some residues within the 13 aa insertion may be critical for the strong sarcomeric localization of Smyd1b_tv1. Sequence comparison with Smyd1b_tv1 orthologs from other vertebrates revealed several highly conserved residues (Phe223, His224 and Gln226) and two potential phosphorylation sites (Thr221 and Ser225) within the 13 aa insertion. To determine whether these residues are involved in the increased sarcomeric localization of Smyd1b_tv1, we mutated these residues into alanine. Substitution of Phe223 or Ser225 with alanine significantly reduced the sarcomeric localization of Smyd1b_tv1. In contrast, other substitutions had no effect. Moreover, replacing Ser225 with threonine (S225T) retained the strong sarcomeric localization of Smyd1b_tv1. CONCLUSION/SIGNIFICANCE:Together, these data indicate that Phe223 and Ser225 are required for the M-line localization of Smyd1b_tv1.
- Published
- 2011
- Full Text
- View/download PDF
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