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The objective of this study was to investigate the effect of Lactobacillus salivarius(L. salivarius) SNK-6 supple-mentation on the laying performance, egg quality, blood parameters, intestinal morphology, and cecal microbial community of laying hens. A total of 432 healthy 30-wk-age laying hens were randomly divided into 3 groups with 6 replicates under the same husbandry and dietary regimes: control (CON); 2.0 × 108CFU/kg L. salivariussupplementation (T1); 2.0 × 109CFU/kg L. salivariussupplementation (T2). The experiment lasted for 10 weeks. The results indicated that the supplementation resulted in a significant reduction in the broken egg and unqualified egg ratios, and a significant increase in the eggshell strength, eggshell relative weight, albumen height, and Haugh units (P< 0.05). The L. salivarius-treated hens exhibited significantly reduced serum malondialdehyde levels (P< 0.05); significantly increased total protein, phosphorus, calcitonin, and immunoglobulin M (P< 0.05); significantly increased cecal secretory immunoglobulin A concentration (P< 0.05); significantly improved villus height (VH) in the duodenum and VH to crypt depth ratio in the jejunum (P< 0.05). The serum globulin and interleukin-1β, immunoglobulin G concentrations, and catalase activity significantly increased in T2 (P< 0.05). Furthermore, the serum interferon-α level in T1 was significantly higher than that of the CON (P< 0.05). The intestinal barrier-related mRNA gene ZO-1, CLDN1, and MUC2expression in the jejunum was significantly upregulated in the T1 and T2 groups (P< 0.05). The Firmicutes/Bacteroidetes ratio was higher and the relative abundances of Flavonifractor and Clostridiales_noname were significantly higher in the T1 group (P< 0.05). In conclusion, dietary supplementation with L. salivariusSNK-6 may improve hen egg quality, serum antioxidant capacity, immune function, and intestinal health.