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There are 176 endemic plants spread in southern Colchis, of which 45 can be used for some medical treatments. The bioecology and detailed phytochemical content of some medicinal plant populations have not been studied so far. The research objectiveis to study the phytochemical content of endemic species of Helleborus caucasicus, Helleborus abchasicus and Ficaria popovii spread in southern Colchis. The research method for the phytochemical content included separation analysis, whichwas performed byusing UPLC-MS (Waters Acquity QDa detector). Three Steroidal glycosideswere isolated from the MeOH extract of the plants of Helleborus caucasicus and Helleborus abchasicus: Hellebrigenin-D-glucose, 20 – Hydroxyecdysone and Hydroxyecdysone – 3 glucoside. Two saponins (Hederagenin 3-O -α-L-arabino pyranoside, Hederagenin28-O-[α-L-rhamno-pyranosyl(1→4)-β-D-glucopyranosyl-(1→6)]β-D-lucopyranoside) and four flavonoids (kaempferol 3-O-β-ᴅ- (6ʺ-α-ʟ-rhamnopyranosyl)-glucopyranoside (nicotiflorin), apigenin 8-C-β-ᴅ-glucopyranoside (vitexin), luteolin 8-C-β-ᴅ-glucopyranoside (orientin), quercetin 3-O-rutinoside) were isolated from the tubers and flowers of Ficaria Popovii. Three Steroidal glycosides and Hydroxyecdysone -3 glucoside were isolated from the MeOH extract of Helleborus caucasicus.In addition, two saponins and four flavonoids were isolated from the tubers and leaves of FicariaPopovii.