Search

Your search keyword '"Xiaowei Zhang"' showing total 3 results
Start Over Author "Xiaowei Zhang" Publication Year Range Last 3 years Journal proceedings of the national academy of sciences of the united states of america
3 results on '"Xiaowei Zhang"'

1. Neuropilin-1, a myeloid cell-specific protein, is an inhibitor of HIV-1 infectivity

Author

Shumei Wang, Li Zhao, Xiaowei Zhang, Jingjing Zhang, Hong Shang, and Guoxin Liang

Subjects
CD4-Positive T-Lymphocytes, Multidisciplinary, dendritic cell, Macrophages, Virion, HIV Infections, Dendritic Cells, macrophage, Biological Sciences, Virus Replication, Microbiology, Neuropilin-1, Cell Line, myeloid cells, HIV-1, Humans, NRP-1
Abstract

Significance Macrophages and dendritic cells represent an important target for HIV-1 replication in vivo as they serve both as a vehicle for virus dissemination throughout the body and a viral reservoir. However, myeloid cells can support persistent replication of HIV-1 and, in contrast to infected T cells, demonstrate lower productivity. Using proteomics, we discovered that NRP-1 is a host restriction factor that inhibits HIV-1 from infecting macrophages and dendritic cells. NRP-1 is incorporated into the HIV-1 virion particle to inhibit its ability to attach to target cells in a viral envelope glycoprotein-independent manner. Overall, these results provide insights into the ability of myeloid lineage cells to utilize NRP-1 to interfere with HIV-1 infection., Myeloid lineage cells such as macrophages and dendritic cells (DCs), targeted by HIV-1, are important vehicles for virus dissemination through the body as well as viral reservoirs. Compared to activated lymphocytes, myeloid cells are collectively more resistant to HIV-1 infection. Here we report that NRP-1, encoding transmembrane protein neuropilin-1, is highly expressed in macrophages and DCs but not CD4+ T cells, serving as an anti-HIV factor to inhibit the infectivity of HIV-1 progeny virions. Silencing NRP-1 enhanced the transmission of HIV-1 in macrophages and DCs significantly and increased the infectivity of the virions produced by these cells. We further demonstrated that NRP-1 was packaged into the progeny virions to inhibit their ability to attach to target cells, thus reducing the infectivity of the virions. These data indicate that NRP-1 is a newly identified antiviral protein highly produced in both macrophages and DCs that inhibit HIV-1 infectivity; thus, NRP-1 may be a novel therapeutic strategy for the treatment of HIV-1 infection.

Published
2021

2. Sensitive bacterial Vm sensors revealed the excitability of bacterial Vm and its role in antibiotic tolerance.

Author

Xin Jin, Xiaowei Zhang, Xuejing Ding, Tian Tian, Chao-Kai Tseng, Xinwei Luo, Xiao Chen, Chien-Jung Lo, Leake, Mark C., and Fan Bai

Subjects
*MEMBRANE potential, *ANTIBIOTICS, *DETECTORS, *BACTERIAL cells, *EUKARYOTIC cells
Abstract

As an important free energy source, the membrane voltage (Vm) regulates many essential physiological processes in bacteria. However, in comparison with eukaryotic cells, knowledge of bacterial electrophysiology is very limited. Here, we developed a set of novel genetically encoded bacterial Vm sensors which allow single-cell recording of bacterial Vm dynamics in live cells with high temporal resolution. Using these new sensors, we reveal the electrically "excitable" and "resting" states of bacterial cells dependent on their metabolic status. In the electrically excitable state, frequent hyperpolarization spikes in bacterial Vm are observed, which are regulated by Na+/K+ ratio of the medium and facilitate increased antibiotic tolerance. In the electrically resting state, bacterial Vm displays significant cell-to-cell heterogeneity and is linked to the cell fate after antibiotic treatment. Our findings demonstrate the potential of our newly developed voltage sensors to reveal the underpinning connections between bacterial Vm and antibiotic tolerance. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

3. Neuropilin-1, a myeloid cell-specific protein, is an inhibitor of HIV-1 infectivity.

Author

Shumei Wang, Li Zhao, Xiaowei Zhang, Jingjing Zhang, Hong Shang, and Guoxin Liang

Subjects
HIV, MYELOID cells, MEMBRANE proteins, DENDRITIC cells, COMMERCIAL products, T cells
Abstract

Myeloid lineage cells such as macrophages and dendritic cells (DCs), targeted by HIV-1, are important vehicles for virus dissemination through the body as well as viral reservoirs. Compared to activated lymphocytes, myeloid cells are collectively more resistant to HIV-1 infection. Here we report that NRP-1, encoding transmembrane protein neuropilin-1, is highly expressed in macrophages and DCs but not CD4+ T cells, serving as an anti-HIV factor to inhibit the infectivity of HIV-1 progeny virions. Silencing NRP-1 enhanced the transmission of HIV-1 in macrophages and DCs significantly and increased the infectivity of the virions produced by these cells. We further demonstrated that NRP-1 was packaged into the progeny virions to inhibit their ability to attach to target cells, thus reducing the infectivity of the virions. These data indicate that NRP-1 is a newly identified antiviral protein highly produced in both macrophages and DCs that inhibit HIV-1 infectivity; thus, NRP-1 may be a novel therapeutic strategy for the treatment of HIV-1 infection. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results