1. SYNPLA, a method to identify synapses displaying plasticity after learning
- Author
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Dore, Kim, Pao, Yvonne, Soria Lopez, Jose, Aronson, Sage, Zhan, Huiqing, Ghosh, Sanchari, Merrill, Sabina, Zador, Anthony M, Malinow, Roberto, and Kebschull, Justus M
- Subjects
Basic Behavioral and Social Science ,Neurosciences ,Behavioral and Social Science ,1.2 Psychological and socioeconomic processes ,Underpinning research ,Neurological ,Mental health ,Animals ,Auditory Cortex ,Cells ,Cultured ,Conditioning ,Psychological ,Geniculate Bodies ,High-Throughput Screening Assays ,Hippocampus ,Learning ,Mice ,Nerve Tissue Proteins ,Neuronal Plasticity ,Protein Interaction Mapping ,Rats ,Synapses ,proximity ligation assay ,synaptic potentiation ,fear conditioning ,defense conditioning ,GluA1 - Abstract
Which neural circuits undergo synaptic changes when an animal learns? Although it is widely accepted that changes in synaptic strength underlie many forms of learning and memory, it remains challenging to connect changes in synaptic strength at specific neural pathways to specific behaviors and memories. Here we introduce SYNPLA (synaptic proximity ligation assay), a synapse-specific, high-throughput, and potentially brain-wide method capable of detecting circuit-specific learning-induced synaptic plasticity.
- Published
- 2020