14 results on '"Li Shen"'
Search Results
2. TGM2-mediated histone transglutamination is dictated by steric accessibility
- Author
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Bradley J. Lukasak, Michelle M. Mitchener, Lingchun Kong, Barbara E. Dul, Cole D. Lazarus, Aarthi Ramakrishnan, Jizhi Ni, Li Shen, Ian Maze, and Tom W. Muir
- Subjects
Histones ,Mammals ,Multidisciplinary ,Glutamine ,Heterochromatin ,Animals ,Protein Glutamine gamma Glutamyltransferase 2 ,DNA ,Chromatin ,Nucleosomes - Abstract
Recent studies have identified serotonylation of glutamine-5 on histone H3 (H3Q5ser) as a novel posttranslational modification (PTM) associated with active transcription. While H3Q5ser is known to be installed by tissue transglutaminase 2 (TGM2), the substrate characteristics affecting deposition of the mark, at the level of both chromatin and individual nucleosomes, remain poorly understood. Here, we show that histone serotonylation is excluded from constitutive heterochromatic regions in mammalian cells. Biochemical studies reveal that the formation of higher-order chromatin structures associated with heterochromatin impose a steric barrier that is refractory to TGM2-mediated histone monoaminylation. A series of structure-activity relationship studies, including the use of DNA–barcoded nucleosome libraries, shows that steric hindrance also steers TGM2 activity at the nucleosome level, restricting monoaminylation to accessible sites within histone tails. Collectively, our data indicate that the activity of TGM2 on chromatin is dictated by substrate accessibility rather than by primary sequence determinants or by the existence of preexisting PTMs, as is the case for many other histone-modifying enzymes.
- Published
- 2023
3. Essential role of poly(ADP-ribosyl)ation in cocaine action
- Author
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Scobie, Kimberly N., Damez-Werno, Diane, Sun, HaoSheng, Shao, NingYi, Gancarz, Amy, Panganiban, Clarisse H., Dias, Caroline, Koo, JaWook, Caiafa, Paola, Kaufman, Lewis, Neve, Rachael L., Dietz, David M., Li Shen, and Nestler, Eric J.
- Published
- 2014
4. Ameliorating parkinsonian motor dysfunction by targeting histamine receptors in entopeduncular nucleus-thalamus circuitry.
- Author
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Jian-Ya Peng, Zeng-Xin Qi, Qi Yan, Xiu-Juan Fan, Kang-Li Shen, Hui-Wei Huang, Jian-Hua Lu, Xiao-Qin Wang, Xiao-Xia Fang, Liming Mao, Jianguang Ni, Liang Chen, and Qian-Xing Zhuang
- Subjects
HISTAMINE receptors ,GENETIC regulation ,PARKINSON'S disease ,ACTION potentials ,SUBTHALAMIC nucleus - Abstract
In Parkinson's disease (PD), reduced dopamine levels in the basal ganglia have been associated with altered neuronal firing and motor dysfunction. It remains unclear whether the altered firing rate or pattern of basal ganglia neurons leads to parkinsonism-associated motor dysfunction. In the present study, we show that increased histaminergic innervation of the entopeduncular nucleus (EPN) in the mouse model of PD leads to activation of EPN parvalbumin (PV) neurons projecting to the thalamic motor nucleus via hyperpolarization-activated cyclic nucleotide-gated (HCN) channels coupled to postsynaptic H2R. Simultaneously, this effect is negatively regulated by presynaptic H3R activation in subthalamic nucleus (STN) glutamatergic neurons projecting to the EPN. Notably, the activation of both types of receptors ameliorates parkinsonism-associated motor dysfunction. Pharmacological activation of H2R or genetic upregulation of HCN2 in EPNPV neurons, which reduce neuronal burst firing, ameliorates parkinsonism-associated motor dysfunction independent of changes in the neuronal firing rate. In addition, optogenetic inhibition of EPNPV neurons and pharmacological activation or genetic upregulation of H3R in EPN-projecting STNGlu neurons ameliorate parkinsonism-associated motor dysfunction by reducing the firing rate rather than altering the firing pattern of EPNPV neurons. Thus, although a reduced firing rate and more regular firing pattern of EPNPV neurons correlate with amelioration in parkinsonism-associated motor dysfunction, the firing pattern appears to be more critical in this context. These results also confirm that targeting H2R and its downstream HCN2 channel in EPNPV neurons and H3R in EPN-projecting STNGlu neurons may represent potential therapeutic strategies for the clinical treatment of parkinsonism-associated motor dysfunction. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
5. Nutrient starvation elicits an acute autophagic response mediated by Ulk1 dephosphorylation and its subsequent dissociation from AMPK
- Author
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Shang, Libin, Chen, She, Du, Fenghe, Li, Shen, Zhao, Liping, and Wang, Xiaodong
- Published
- 2011
6. TGM2-mediated histone transglutamination is dictated by steric accessibility.
- Author
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Lukasak, Bradley J., Mitchener, Michelle M., Lingchun Kong, Dul, Barbara E., Lazarus, Cole D., Ramakrishnan, Aarthi, Jizhi Ni, Li Shen, Maze, Ian, and Muir, Tom W.
- Subjects
POST-translational modification ,HETEROCHROMATIN ,STRUCTURE-activity relationships ,STERIC hindrance ,CHROMATIN - Abstract
Recent studies have identified serotonylation of glutamine-5 on histone H3 (H3Q5ser) as a novel posttranslational modification (PTM) associated with active transcription. While H3Q5ser is known to be installed by tissue transglutaminase 2 (TGM2), the substrate characteristics affecting deposition of the mark, at the level of both chromatin and individual nucleosomes, remain poorly understood. Here, we show that histone serotonylation is excluded from constitutive heterochromatic regions in mammalian cells. Biochemical studies reveal that the formation of higher-order chromatin structures associated with heterochromatin impose a steric barrier that is refractory to TGM2-mediated histone monoaminylation. A series of structure-activity relationship studies, including the use of DNA-barcoded nucleosome libraries, shows that steric hindrance also steers TGM2 activity at the nucleosome level, restricting monoaminylation to accessible sites within histone tails. Collectively, our data indicate that the activity of TGM2 on chromatin is dictated by substrate accessibility rather than by primary sequence determinants or by the existence of preexisting PTMs, as is the case for many other histone-modifying enzymes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
7. Chromatin remodelers and lineage-specific factors interact to target enhancers to establish proneurosensory fate within otic ectoderm.
- Author
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Jinshu Xu, Jun Li, Ting Zhang, Huihui Jiang, Ramakrishnan, Aarthi, Fritzsch, Bernd, Li Shen, and Pin-Xian Xu
- Subjects
ECTODERM ,CHROMATIN-remodeling complexes ,CHROMATIN ,SENSORY neurons ,TRANSCRIPTION factors ,COMMERCIAL products - Abstract
Specification of Sox2
+ proneurosensory progenitors within otic ectodermis a prerequisite for the production of sensory cells and neurons for hearing. However, the underlying molecular mechanisms driving this lineage specification remain unknown. Here, we show that the Brg1-based SWI/SNF chromatin-remodeling complex interacts with the neurosensory-specific transcriptional regulators Eya1/Six1 to induce Sox2 expression and promote proneurosensory-lineage specification. Ablation of the ATPase-subunit Brg1 or both Eya1/Six1 results in loss of Sox2 expression and lack of neurosensory identity, leading to abnormal apoptosis within the otic ectoderm. Brg1 binds to two of three distal 3' Sox2 enhancers occupied by Six1, and Brg1-binding to these regions depends on Eya1-Six1 activity. We demonstrate that the activity of these Sox2 enhancers in otic neurosensory cells specifically depends on binding to Six1. Furthermore, genome-wide and transcriptome profiling indicate that Brg1 may suppress apoptotic factor Map3k5 to inhibit apoptosis. Together, our findings reveal an essential role for Brg1, its downstream pathways, and their interactions with Six1/Eya1 in promoting proneurosensory fate induction in the otic ectoderm and subsequent neuronal lineage commitment and survival of otic cells. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
8. Divergent roles of astrocytic versus neuronal EAAT2 deficiency on cognition and overlap with aging and Alzheimer’s molecular signatures.
- Author
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Sharma, Abhijeet, Kazim, Syed Faraz, Larson, Chloe S., Ramakrishnan, Aarthi, Gray, Jason D., McEwen, Bruce S., Rosenberg, Paul A., Li Shen, and Pereira, Ana C.
- Subjects
GLUTAMATE transporters ,GENE expression profiling ,EXCITATORY amino acids ,LONG-term memory ,SHORT-term memory - Abstract
The excitatory amino acid transporter 2 (EAAT2) is the major glutamate transporter in the brain expressed predominantly in astrocytes and at low levels in neurons and axonal terminals. EAAT2 expression is reduced in aging and sporadic Alzheimer’s disease (AD) patients’ brains. The role EAAT2 plays in cognitive aging and its associated mechanisms remains largely unknown. Here, we show that conditional deletion of astrocytic and neuronal EAAT2 results in age-related cognitive deficits. Astrocytic, but not neuronal EAAT2, deletion leads to early deficits in short-term memory and in spatial reference learning and long-term memory. Neuronal EAAT2 loss results in late-onset spatial reference longterm memory deficit. Neuronal EAAT2 deletion leads to dysregulation of the kynurenine pathway, and astrocytic EAAT2 deficiency results in dysfunction of innate and adaptive immune pathways, which correlate with cognitive decline. Astrocytic EAAT2 deficiency also shows transcriptomic overlaps with human aging and AD. Overall, the present study shows that in addition to the widely recognized astrocytic EAAT2, neuronal EAAT2 plays a role in hippocampus-dependent memory. Furthermore, the gene expression profiles associated with astrocytic and neuronal EAAT2 deletion are substantially different, with the former associated with inflammation and synaptic function similar to changes observed in human AD and gene expression changes associated with inflammation similar to the aging human brain. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
9. Suppression of RGSz1 function optimizes the actions of opioid analgesics by mechanisms that involve the Wnt/β-catenin pathway.
- Author
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Gaspari, Sevasti, Purushothaman, Immanuel, Cogliani, Valeria, Sakloth, Farhana, Neve, Rachael L., Howland, David, Ring, Robert H., Ross, Elliott M., Li Shen, and Zachariou, Venetia
- Subjects
REGULATOR of G-protein-signaling proteins ,OPIOID analgesics ,WNT signal transduction ,MORPHINE ,DRUG tolerance ,PERIAQUEDUCTAL gray matter ,RNA sequencing ,PAIN management - Abstract
Regulator of G protein signaling z1 (RGSz1), a member of the RGS family of proteins, is present in several networks expressing mu opioid receptors (MOPRs). By using genetic mousemodels for global or brain region-targeted manipulations of RGSz1 expression, we demonstrated that the suppression of RGSz1 function increases the analgesic efficacy of MOPR agonists in male and female mice and delays the development ofmorphine tolerancewhile decreasing the sensitivity to rewarding and locomotor activating effects. Using biochemical assays and next-generation RNA sequencing, we identified a key role of RGSz1 in the periaqueductal gray (PAG) in morphine tolerance. Chronic morphine administration promotes RGSz1 activity in the PAG, which in turn modulates transcription mediated by the Wnt/ß-catenin signaling pathway to promote analgesic tolerance to morphine. Conversely, the suppression of RGSz1 function stabilizes Axin2-Gaz complexes near the membrane and promotes ß-catenin activation, thereby delaying the development of analgesic tolerance. These data show that the regulation of RGS complexes, particularly those involving RGSz1-Gaz, represents a promising target for optimizing the analgesic actions of opioids without increasing the risk of dependence or addiction. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
10. Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia.
- Author
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Li Shen, Zhenyu Diao, Hai-Xiang Sun, Gui-Jun Yan, Zhiqun Wang, Ruo-Tian Li, Yimin Dai, Jie Li, Hailing Ding, Guangfeng Zhao, Mingming Zheng, Pingping Xue, Mo Liu, Yali Hu, Jingmei Wang, and Yan Zhou
- Subjects
- *
PREECLAMPSIA , *RISK factors of preeclampsia , *CD81 antigen , *ENDOTHELIAL cells , *PREGNANCY complications , *GENETICS - Abstract
Preeclampsia (PE) is initiated by abnormal placentation in the early stages of pregnancy, followed by systemic activation of endothelial cells of the maternal small arterioles in the late second or third trimester (TM) of pregnancy. During normal pregnancy, placental cytotrophoblasts (CTBs) invade the maternal uterine wall and spiral arteries, whereas this process is interrupted in PE. However, it is not known how the malformed placenta triggers maternal endothelial crisis and the associated manifestations. Here, we have focused on the association of CD81 with PE. CD81, a member of the tetraspanin superfamily, plays significant roles in cell growth, adhesion, and motility. The function of CD81 in human placentation and its association with pregnancy complications are currently unknown. In the present study, we have demonstrated that CD81 was preferentially expressed in normal first TM placentas and progressively downregulated with gestation advance. In patients with early-onset severe PE (sPE), CD81 expression was significantly up-regulated in syncytiotrophoblasts (STBs), CTBs and the cells in the villous core. In addition, high levels of CD81 were observed in the maternal sera of patients with sPE. Overexpressing CD81 in CTBs significantly decreased CTB invasion, and culturing primary human umbilical vein endothelial cells (HUVECs) in the presence of a high dose of exogenous CD81 resulted in interrupted angiogenesis and endothelial cell activation in vitro. Importantly, the phenotype of human PE was mimicked in the CD81-induced rat model. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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11. Aberrant H3.3 dynamics in NAc promote vulnerability to depressive-like behavior.
- Author
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Lepack, Ashley E., Bagot, Rosemary C., Peña, Catherine J., Loh, Yong-Hwee E., Farrelly, Lorna A., Yang Lu, Powell, Samuel K., Lorsch, Zachary S., Issler, Orna, Cates, Hannah M., Tamming, Carol A., Molina, Henrik, Nestler, Eric J., Allis, C. David, Maze, Ian, and Li Shen
- Subjects
MENTAL depression ,AFFECTIVE disorders ,PATHOLOGICAL physiology ,GENE expression ,TRANSCRIPTION factors - Abstract
Human major depressive disorder (MDD), along with related mood disorders, is among the world's greatest public health concerns; however, its pathophysiology remains poorly understood. Persistent changes in gene expression are known to promote physiological aberrations implicated in MDD. More recently, histone mechanisms affecting cell type- and regional-specific chromatin structures have also been shown to contribute to transcriptional programs related to depressive behaviors, as well as responses to antidepressants. Although much emphasis has been placed in recent years on roles for histone posttranslational modifications and chromatin-remodeling events in the etiology of MDD, it has become increasingly clear that replication-independent histone variants (e.g., H3.3), which differ in primary amino acid sequence from their canonical counterparts, similarly play critical roles in the regulation of activity-dependent neuronal transcription, synaptic connectivity, and behavioral plasticity. Here, we demonstrate a role for increased H3.3 dynamics in the nucleus accumbens (NAc)--a key limbic brain reward region--in the regulation of aberrant social stress-mediated gene expression and the precipitation of depressive-like behaviors in mice. We find that molecular blockade of these dynamics promotes resilience to chronic social stress and results in a partial renormalization of stress-associated transcriptional patterns in the NAc. In sum, our findings establish H3.3 dynamics as a critical, and previously undocumented, regulator of mood and suggest that future therapies aimed at modulating striatal histone dynamics may potentiate beneficial behavioral adaptations to negative emotional stimuli. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
12. ATRX tolerates activity-dependent histone H3 methyl/phos switching to maintain repetitive element silencing in neurons.
- Author
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Kyung-Min Noh, Maze, Ian, Dan Zhao, Bin Xiang, Wenderski, Wendy, Lewis, Peter W., Li Shen, Haitao Li, and David Allis, C.
- Subjects
X-linked intellectual disabilities ,LYSINE metabolism ,METHYLATION ,HETEROCHROMATIN ,CRYSTAL structure ,PHOSPHORYLATION ,HISTONES - Abstract
ATRX (the alpha thalassemia/mental retardation syndrome X-linked protein) is a member of the switch2/sucrose nonfermentable2 (SWI2/SNF2) family of chromatin-remodeling proteins and primarily functions at heterochromatic loci via its recognition of "repressive" histone modifications [e.g., histone H3 lysine 9 tri-methylation (H3K9me3)]. Despite significant roles for ATRX during normal neural development, as well as its relationship to human disease, ATRX function in the central nervous system is not well understood. Here, we describe ATRX's ability to recognize an activity-dependent combinatorial histone modification, histone H3 lysine 9 tri-methylation/ serine 10 phosphorylation (H3K9me3S10ph), in postmitotic neurons. In neurons, this "methyl/phos" switch occurs exclusively after periods of stimulation and is highly enriched at heterochromatic repeats associated with centromeres. Using a multifaceted approach, we reveal that H3K9me3S10ph-bound Atrx represses noncoding transcription of centromeric minor satellite sequences during instances of heightened activity. Our results indicate an essential interaction between ATRX and a previously uncharacterized histone modification in the central nervous system and suggest a potential role for abnormal repetitive element transcription in pathological states manifested by ATRX dysfunction. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
13. Cocaine dynamically regulates heterochromatin and repetitive element unsilencing in nucleus accumbens.
- Author
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Maze, Ian, Jian Feng, Wilkinson, Matthew B., HaoSheng Sun, Li Shen, and Nestler, Eric J.
- Subjects
LOCAL anesthetics ,DRUG abuse ,CHROMATIN ,NUCLEOPROTEINS ,BACTERIAL chromatin ,CHROMOSOMES - Abstract
Repeated cocaine exposure induces persistent alterations in genome-wide transcriptional regulatory networks, chromatin remodeling activity and, ultimately, gene expression profiles in the brain's reward circuitry. Virtually all previous investigations have centered on drug-mediated effects occurring throughout active euchromatic regions of the genome, with very little known concerning the impact of cocaine exposure on the regulation and maintenance of heterochromatin in adult brain. Here, we report that cocaine dramatically and dynamically alters heterochromatic histone H3 lysine 9 trimethylation (H3K9me3) in the nucleus accumbens (NAc), a key brain reward region. Furthermore, we demonstrate that repeated cocaine exposure causes persistent decreases in heterochromatization in this brain region, suggesting a potential role for heterochromatic regulation in the long-term actions of cocaine. To identify precise genomic loci affected by these alterations, chromatin immunoprecipitation followed by massively parallel DNA sequencing (ChIP-Seq) was performed on NAc. ChIP-Seq analyses confirmed the existence of the H3K9me3 mark mainly within intergenic regions of the genome and identified specific patterns of cocaine-induced H3K9me3 regulation at repetitive genomic sequences. Cocaine-mediated decreases in H3K9me3 enrichment at specific genomic repeats [e.g., long interspersed nuclear element (LINE)-1 repeats] were further confirmed by the increased expression of LINE-1 retrotransposon-associated repetitive elements in NAc. Such increases likely reflect global patterns of genomic destabilization in this brain region after repeated cocaine administration and open the door for future investigations into the epigenetic and genetic basis of drug addiction. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
14. A commonly carried allele of the obesity-related FTO gene is associated with reduced brain volume in the healthy elderly.
- Author
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Ho, April J., Stein, Jason L., Xue Hua, Suh Lee, Hibar, Derrek P., Leowa, Alex D., Dinov, Ivo D., Toga, Arthur W., Saykin, Andrew J., Li Shen, Foroud4, Tatiana, Pankratz, Nathan, Huentelman, Matthew J., Craig, David W., Gerber, Jill D., Allen, April N., Corneveaux, Jason J., Stephan, Dietrich A., deCarli, Charles S., and DeChairo, Bryan M.
- Subjects
GENE frequency ,OBESITY ,TRANS men ,GENETIC regulation ,POPULATION aging ,ISOPENTENOIDS ,LOW-cholesterol diet - Abstract
A recently identified variant within the fat mass and obesity- associated (FTO) gene is carried by 46% of Western Europeans and is associated with an ∼1.2 kg higher weight, on average, in adults and an ∼1 cm greater waist circumference. With >1 billion overweight and 300 million obese persons worldwide, it is crucial to understand the implications of carrying this very common allele for the health of our aging population. FTO is highly expressed in the brain and elevated body mass index (BMI) is associated with brain atrophy, but it is unknown how the obesity-associated risk allele affects human brain structure. We therefore generated 3D maps of regional brain volume differences in 206 healthy elderly subjects scanned with MRI and genotyped as part of the Alzheimer's Disease Neuroimaging Initiative. We found a pattern of systematic brain volume deficits in carriers of the obesity-associated risk allele versus noncarriers. Relative to structure volumes in the mean template, FTO risk allele carriers versus noncarriers had an average brain volume difference of ∼8% in the frontal lobes and 12% in the occipital lobes-these regions also showed significant volume deficits in subjects with higher BMI. These brain differences were not attributable to differences in cholesterol levels, hypertension, or the volume of white matter hyperintensities; which were not detectably higher in FTO risk allele carriers versus noncarriers. These brain maps reveal that a commonly carried susceptibility allele for obesity is associated with structural brain atrophy, with implications for the health of the elderly. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
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