1. Pattern reorganization occurs independently of cell division during Drosophila wing disc regeneration in situ.
- Author
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Díaz-García S and Baonza A
- Subjects
- Animals, Animals, Genetically Modified, Cell Division physiology, Cell Proliferation, Drosophila Proteins genetics, Drosophila Proteins metabolism, Drosophila melanogaster genetics, Drosophila melanogaster metabolism, Drosophila melanogaster physiology, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Imaginal Discs cytology, Imaginal Discs metabolism, Immunohistochemistry, Microscopy, Fluorescence, Models, Biological, RNA Interference, Regeneration genetics, Time Factors, Wings, Animal injuries, Wings, Animal metabolism, Wnt1 Protein genetics, Wnt1 Protein metabolism, Wound Healing genetics, Imaginal Discs physiology, Regeneration physiology, Wings, Animal physiopathology, Wound Healing physiology
- Abstract
One of the most intriguing problems in developmental biology is how an organism can replace missing organs or portions of its body after injury. This capacity, known as regeneration, is conserved across different phyla. The imaginal discs of Drosophila melanogaster provide a particularly well-characterized model for analyzing regeneration. We have developed a unique method to study organ regeneration under physiological conditions using the imaginal discs of Drosophila. Using this method, we revisited different aspects of organ regeneration. The results presented in this report suggest that during the initial stages of regeneration, different processes occur, including wound healing, a temporary loss of markers of cell-fate commitment, and pattern reorganization. We present evidence indicating that all of these processes occur even when cell division has been arrested. Our data also suggested that Wingless is not required during the early stages of disc regeneration.
- Published
- 2013
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