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Your search keyword '"Stöger E"' showing total 2 results
Start Over Author "Stöger E" Journal proceedings of the national academy of sciences of the united states of america
2 results on '"Stöger E"'

1. Pollen selection: a transgenic reconstruction approach.

Author

Touraev, A, Fink, C S, Stöger, E, and Heberle-Bors, E

Abstract

A transgenic reconstruction experiment has been performed to determine the feasibility of male gametophytic selection to enhance transmission of genes to the next sporophytic generation. For tobacco pollen from a transgenic plant containing a single hygromycin-resistance (hygromycin phosphotransferase, hpt-) gene under control of the dc3 promoter, which is active in both sporophytic and gametophytic tissues, 3 days of in vitro maturation in hygromycin-containing medium was sufficient to result in a 50% reduction of germinating pollen, as expected for meiotic segregation of a single locus insert. Pollination of wild-type plants with the selected pollen yielded 100% transgenic offspring, as determined by the activity of the linked kanamycin-resistance gene--present within the same transferred T-DNA borders--under control of the nos promoter. This is direct proof that selection acting on male gametophytes can be a means to alter the frequency of genes in the progeny.

Published
1995
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2. Cost-effective production of a vaginal protein microbicide to prevent HIV transmission.

Author

Ramessar K, Rademacher T, Sack M, Stadlmann J, Platis D, Stiegler G, Labrou N, Altmann F, Ma J, Stöger E, Capell T, and Christou P

Subjects
Administration, Intravaginal, Animals, Anti-Infective Agents immunology, Antibodies, Monoclonal isolation & purification, Chromatography, Affinity, Cost-Benefit Analysis, Cricetinae, Cricetulus, Drug Administration Routes, Electrophoresis, Polyacrylamide Gel, Glycopeptides chemistry, HIV Antibodies isolation & purification, HIV Antigens immunology, HIV Infections immunology, Humans, Mass Spectrometry, Molecular Weight, Neutralization Tests, Plants, Genetically Modified, Polysaccharides analysis, Seeds metabolism, Staphylococcal Protein A metabolism, Zea mays genetics, Anti-Infective Agents chemical synthesis, Anti-Infective Agents economics, Antibodies, Monoclonal biosynthesis, HIV Antibodies biosynthesis, HIV Infections prevention & control, HIV Infections transmission
Abstract

A series of small-molecule microbicides has been developed for vaginal delivery to prevent heterosexual HIV transmission, but results from human clinical trials have been disappointing. Protein-based microbicides, such as HIV-specific monoclonal antibodies, have been considered as an alternative approach. Despite their promising safety profile and efficacy, the major drawback of such molecules is the economy of large-scale production in mammalian cells, the current system of choice. Here, we show that an alternative biomanufacturing platform is now available for one of the most promising anti-HIV antibodies (2G12). Our data show that the HIV-neutralization capability of the antibody is equal to or superior to that of the same antibody produced in CHO cells. We conclude that this protein production system may provide a means to achieve microbicide ingredient manufacture at costs that would allow product introduction and manufacture in the developing world.

Published
2008
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